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VPS4A (NM_013245) Human Untagged Clone
CAT＃: SC115346
VPS4A (untagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CIMDAG; SKD1; SKD1A; SKD2; VPS4; VPS4-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115346 sequence for NM_013245 edited (data generated by NextGen Sequencing)
ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC ATCAGGAGGAGGTTTGAAAAGCGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGTTAA Clone variation with respect to NM_013245.2 861 a=>g >OriGene 5' read for NM_013245 unedited
GTTCGGATTTTGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGAC CGAGGCCGCAAGCAGCGCCGCGGGGTGTGGGGCGGACCCAGGAGATGAAATGACAACGTC AACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGACAAAGCCAAGAA CTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTCCACGCTATCAA GTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGCGTGCAGTACCT AGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACACGGCAAGAAGCC AGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAAGGGGATAATCC GGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAGAAGCCCAACAT ACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAAGAAGCTGTCAT TTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGGCGGGGGATTCT GCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTGGCAACAGAGGC CAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCACATGGCTGGGGGA GAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGGCCAGCAGCACAAGCCCTCCATCAT CTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAATGAGAGTGAGGCCGNC CGGAGGATCAAAACGGAGTTCTTGGTCCAATGCAGGGGGGTGGGGGAAATACAATGATGG NACTCTGGTTCCTTGGAGCCACAACTAACTCGGGGGGG >OriGene 3' read for NM_013245 unedited
ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTAGCTCAAAAAGGGAAAAGGATTTATTAAACCATTTCCAGGACAGGGACCCAGG GGCAGTGGCTGGGTTCAGTGTTCTCACCCTCAAAGGAAAAAAAGGCAGTGGTGAACACCC GCCTGACTGCCTTACCCTGATCTAAACGACACCATTTCCTCCCCCAAGAAAAACACAGCC CTGGATGTGAACCTAGGAGGGTGGGAATGGCTTATGTCACTCGGTCTTTGAGGCAAAACT TGGGCTGGGGTTGCAGGGGCATCAACCAATCTGCTCTCAAGGAGCAAAACTTGGGCGTAA AAGGAACGGGCAGTGGGGCTAGCCGGCTGTCTGGAGGTCTGGGTGACCTGGCAACACGGG TGTTTTTCCCCCGCCACCCCGCCCCACTTCTTGACACTTCCAGGTGATTTTATCTTTATA AATAAGATGAAAATCCAAGCCGCATTAATTTAGGGGAACAAAAGATGGAAAAAAAAAGGG GGGGAGGCAGAAAAAGCTGATGAGCCTCCAGGAGAGGAGGAAGTAGGAAGAGCAGTGTCC CCTGTGTGCCCCAGGGCAGTGGAGTGTCTCTGTGGCTGAAGGCCCTTCCTTCTGGCTCCC TGGCAGACGGCCAGACAGCAGAGGGAACTCTGTATTGACTGGGACAAGCCCTGNAGCCCT GTCTGGCTGTTGACATGGGGAAGCCTGGATTTGCCCAATTACCTTCCACTTCCCATTGCC CAGTGAGCAGCTTTTACTCTCTGGCCAAAGTCTTGAGAATTCTTCACTTTCAGAGGCGCT GCATCACGTGGGCGGNTGGGGNCAAAACGCANTTGTCCACTGCAACCCAGTTTAAATTTG CCCAAGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013245 |
| Insert Size | 2090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013245.2, NP_037377.1 |
| RefSeq Size | 2211 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 27183 |
| UniProt ID | Q9UN37 |
| Domains | AAA, AAA, MIT |
| Protein Pathways | Endocytosis |
| Gene Summary | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208099 | VPS4A (Myc-DDK-tagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A) |
CNY 3,656.00 |
|
| RC208099L1 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208099L2 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), mGFP tagged |
CNY 5,890.00 |
|
| RC208099L3 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208099L4 | Lenti ORF clone of Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A), mGFP tagged |
CNY 6,056.00 |
|
| RG208099 | VPS4A (tGFP-tagged) - Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A) |
CNY 5,256.00 |
