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BACE1 (NM_012104) Human Untagged Clone
CAT＃: SC115547
BACE1 (untagged)-Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a
CNY 3,736.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASP2; BACE; HSPC104 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC115547 representing NM_012104.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCAAGCCCTGCCCTGGCTCCTGCTGTGGATGGGCGCGGGAGTGCTGCCTGCCCACGGCACCCAG CACGGCATCCGGCTGCCCCTGCGCAGCGGCCTGGGGGGCGCCCCCCTGGGGCTGCGGCTGCCCCGGGAG ACCGACGAAGAGCCCGAGGAGCCCGGCCGGAGGGGCAGCTTTGTGGAGATGGTGGACAACCTGAGGGGC AAGTCGGGGCAGGGCTACTACGTGGAGATGACCGTGGGCAGCCCCCCGCAGACGCTCAACATCCTGGTG GATACAGGCAGCAGTAACTTTGCAGTGGGTGCTGCCCCCCACCCCTTCCTGCATCGCTACTACCAGAGG CAGCTGTCCAGCACATACCGGGACCTCCGGAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAA GGGGAGCTGGGCACCGACCTGGTAAGCATCCCCCATGGCCCCAACGTCACTGTGCGTGCCAACATTGCT GCCATCACTGAATCAGACAAGTTCTTCATCAACGGCTCCAACTGGGAAGGCATCCTGGGGCTGGCCTAT GCTGAGATTGCCAGGCCTGACGACTCCCTGGAGCCTTTCTTTGACTCTCTGGTAAAGCAGACCCACGTT CCCAACCTCTTCTCCCTGCAGCTTTGTGGTGCTGGCTTCCCCCTCAACCAGTCTGAAGTGCTGGCCTCT GTCGGAGGGAGCATGATCATTGGAGGTATCGACCACTCGCTGTACACAGGCAGTCTCTGGTATACACCC ATCCGGCGGGAGTGGTATTATGAGGTGATCATTGTGCGGGTGGAGATCAATGGACAGGATCTGAAAATG GACTGCAAGGAGTACAACTATGACAAGAGCATTGTGGACAGTGGCACCACCAACCTTCGTTTGCCCAAG AAAGTGTTTGAAGCTGCAGTCAAATCCATCAAGGCAGCCTCCTCCACGGAGAAGTTCCCTGATGGTTTC TGGCTAGGAGAGCAGCTGGTGTGCTGGCAAGCAGGCACCACCCCTTGGAACATTTTCCCAGTCATCTCA CTCTACCTAATGGGTGAGGTTACCAACCAGTCCTTCCGCATCACCATCCTTCCGCAGCAATACCTGCGG CCAGTGGAAGATGTGGCCACGTCCCAAGACGACTGTTACAAGTTTGCCATCTCACAGTCATCCACGGGC ACTGTTATGGGAGCTGTTATCATGGAGGGCTTCTACGTTGTCTTTGATCGGGCCCGAAAACGAATTGGC TTTGCTGTCAGCGCTTGCCATGTGCACGATGAGTTCAGGACGGCAGCGGTGGAAGGCCCTTTTGTCACC TTGGACATGGAAGACTGTGGCTACAACATTCCACAGACAGATGAGTCAACCCTCATGACCATAGCCTAT GTCATGGCTGCCATCTGCGCCCTCTTCATGCTGCCACTCTGCCTCATGGTGTGTCAGTGGCGCTGCCTC CGCTGCCTGCGCCAGCAGCATGATGACTTTGCTGATGACATCTCCCTGCTGAAGTGA >OriGene 5' read for NM_012104 unedited
GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCACCCAGACTTGGGGG CAGGCGCCAGGGACGGACGTGGGCCAGTGCGAGCCCAGAGGGCCCGAAGGCCGGGGCCCA CCATGGCCCAAGCCCTGCCCTGGCTCCTGCTGTGGATGGGCGCGGGAGTGCTGCCTGCCC ACGGCACCCAGCACGGCATCCGGCTGCCCCTGCGCAGCGGCCTGGGGGGCGCCCCCCTGG GGCTGCGGCTGCCCCGGGAGACCGACGAAGAGCCCGAGGAGCCCGGCCGGAGGGGCAGCT TTGTGGAGATGGTGGACAACCTGAGGGGCAAGTCGGGGCAGGGCTACTACGTGGAGATGA CCGTGGGCAGCCCCCCGCAGACGCTCAACATCCTGGTGGATACAGGCAGCAGTAACTTTG CAGTGGGTGCTGCCCCCCACCCCTTCCTGCATCGCTACTACCAGAGGCAGCTGTCCAGCA CATACCGGGACCTCCGGAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAAGGGG AGCTGGGCACCGACCTGGTAAGCATCCCCCATGGCCCCAACGTCACTGTGCGTGCCAACA TTGCTGCCATCACTGAATCAGACAAGTTCTTCATCAACGGCTCCAACTGGGAAGGCATCC TGGGGCTGGCCTATGCTGAGATTGCCAGGCCTGACGACTCCCTGGAGCCTTTCTTTGACT CTCTGGTAAAGCAGACCCACGTTCCCAACCTCTTCTCCCTGCAGCTTTGTGGGTGCCTGG CCTTCCCCTCCACCAGTCTGAAGTGCTGTCCTCTGTCGGAGGGAGCATGATCATTGGAGG TATCGACCACTCCCTGTACACAAGCAGTCTCTGGTATACACCCATCCGCGGGAGTGTATT ATGAAGGGATCATTTGGCGGTGGAACATCATGGACACGACTG >OriGene 3' read for NM_012104 unedited
CTTACTTGNNACCGCGGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAAACT GACTCAGTATTCTTGTTTTATTGGTTTATGGCTTGTGGGCAGCTGGCTGAGGCCAGGAAC ACCAGAGGCCCTCCTTGTATTTCCCTCTCCCTTATCAGGTAATTCCACACTTAGCAGGTC CCATACCTGGAAGCAGCGGGTTGACCAGGTAGGAGTAGGATGCAGTGGGTATACTAGGAA AACCCAGCTCTTCCCACTAACACTGGTACCGTAGTTTACTATGCTAGAACTGATAGGGAG GTAATGAACTTGGTCCCCACTACCTAGCCCAAGAACCAGCCTGGGTACCCACGTACCATT AGGAGCGGGGAGGGGAAGAACAGGATAGGGGAAAAAGATGACCTTCATCAAGGAGCTCTT GTGGTGGAGGACATAAGGAAGCCCTGAGGCTGCCATCCTTTCTCCAGGCAGCCTTGATAC TGAAGGCAGTAATGTTAGAGCCATAAAGCACTGTATTTCTCATACTCTTGGTATAATGCT AGTGCCACTGTGTGATGCTTTAGTCAAATAAATGGAGGGGCCATTANAGAGGGTAGGAAG GCAATGAAACCACTCTGAATTATGTAAAGATCTTGCTACTTCTTATAGCCTAATAGGAAG AAATCCAGGCAGGGGTTATAGGGCATCAGCTGTTGAATGAATGTTCAACATTATGCTNTT AAGGATTAGATAGAAGAAGTCTNCTACCTATTCCTTTTAGCACCTGGNCTGCAAAGCTCC AGAGGAAGAAATTGGGGAAAGAGTGAGCCTATGACCCAGAAGACAGAGCCCAGCCCTGCT ATAGTCCAGTTGCTCTCCCACAGGCACCTGGAGCTTAGGGGGNNTAGAATTTCAGTGCTG CCTTCATATGTAGATAAAGGGTGGTCAGAAGAGACATGAAATTATATGCNGAAATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012104 |
| Insert Size | 1506 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012104.3 |
| RefSeq Size | 5850 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 23621 |
| UniProt ID | P56817 |
| Domains | asp |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Protein Pathways | Alzheimer's disease |
| MW | 55.8 kDa |
| Gene Summary | This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (a) is the longest transcript and it encodes the longest isoform (A). |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AßPP into Amyloidogenic Processing Pathway
,Fisher, CL;Resnick, RJ;De, S;Acevedo, LA;Lu, KP;Schroeder, FC;Nicholson, LK;,
J. Alzheimers Dis.
,PubMed ID 27662285
[BACE1]
|
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F-box only protein 2 (Fbxo2) Regulates Amyloid Precursor Levels and Processing
,Atkin, G;Hunt, J;Minakawa, E;Sharkey, L;Tipper, N;Tennant, W;Paulson, HL;,
J. Biol. Chem., Jan 2014.
,PubMed ID 24469452
[BACE1]
|
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The Role of F-box Only Protein 2 (Fbxo2) in Amyloid Precursor Protein Processing and Synaptic Dynamics
,Atkin, GM;,
Thesis
[BACE1]
|
|
miR-107 Regulates Granulin/Progranulin with Implications for Traumatic Brain Injury and Neurodegenerative Disease
,Wang-Xia Wang, Bernard R. Wilfred, Sindhu K. Madathil, Guiliang Tang, Yanling Hu, James Dimayuga, Arnold J. Stromberg, Qingwei Huang, Kathryn E. Saatman, and Peter T. Nelson,
Am. J. Pathol., Jul 2010; 177: 334 - 345
[BACE1]
|
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Receptor- and Non-Receptor Tyrosine Kinases Induce Processing of the Amyloid Precursor Protein: Role of the Low-Density Lipoprotein Receptor-Related Protein
,Giuseppina Minopolia, Fabiana Passaroa, Luigi Aloiaa, Francesca Carlomagnob, Rosa Marina Melillob, Massimo Santorob, Floriana Forzatia, Nicola Zambranoa, Tommaso Russo,
Neurodegenerative Dis 2007;4:94-100 (DOI: 10.1159/000101833)
[BACE1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209115 | BACE1 (Myc-DDK-tagged)-Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a |
CNY 3,728.00 |
|
| RC209115L1 | Lenti ORF clone of Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a, Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC209115L2 | Lenti ORF clone of Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a, mGFP tagged |
CNY 6,460.00 |
|
| RC209115L3 | Lenti ORF clone of Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a, Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC209115L4 | Lenti ORF clone of Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a, mGFP tagged |
CNY 6,128.00 |
|
| RG209115 | BACE1 (tGFP-tagged) - Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a |
CNY 5,320.00 |
