POLR3F (NM_006466) Human Untagged Clone
CAT#: SC116048
POLR3F (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | C34; RPC6; RPC39 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_006466, the custom clone sequence may differ by one or more nucleotides
ATGGCGGAGGTGAAGGTGAAGGTGCAGCCGCCTGACGCGGATCCGGTCGAAATAGAAAACAGGATTATAG AATTATGTCACCAGTTCCCTCATGGAATCACAGACCAAGTAATTCAGAATGAAATGCCTCATATAGAAGC CCAGCAGCGGGCAGTAGCCATCAATAGGTTGTTGTCTATGGGTCAGTTGGATCTCTTAAGGAGCAATACG GGCCTTTTATATAGAATAAAGGACTCTCAGAATGCTGGTAAAATGAAGGGATCCGATAACCAAGAAAAAC TAGTATATCAAATCATAGAGGATGCAGGAAATAAAGGAATATGGAGCAGAGATATCCGCTATAAAAGTAA TTTGCCATTAACAGAAATCAACAAAATTCTGAAGAATCTGGAAAGTAAAAAGCTTATCAAAGCTGTTAAG TCTGTAGCAGCCTCAAAAAAGAAGGTGTATATGCTCTATAACCTGCAGCCAGACCGGTCTGTGACTGGTG GAGCCTGGTACAGTGACCAGGATTTTGAATCTGAATTTGTAGAGGTGCTTAACCAACAGTGTTTTAAATT CCTACAGTCCAAGGCAGAAACAGCACGAGAAAGCAAACAGAACCCAATGATACAAAGAAATAGTTCATTT GCCTCATCACATGAAGTGTGGAAATATATCTGCGAATTGGGAATCAGTAAGGTAGAGTTATCCATGGAAG ACATTGAAACCATCCTGAATACACTCATTTATGATGGAAAAGTGGAGATGACGATTATTGCTGCAAAAGA AGGCACAGTTGGCAGTGTAGATGGACACATGAAACTGTACAGGGCAGTCAATCCAATCATCCCTCCCACA GGTTTGGTCCGGGCACCCTGTGGACTCTGCCCGGTTTTTGATGACTGCCACGAAGGTGGTGAGATTTCAC CATCTAACTGTATTTACATGACAGAGTGGCTCGAATTTTAA >OriGene 5' read for NM_006466 unedited
TTCGCATTTGAATACGTTTCTATAGGCGGCCGCGNAATTCGCACGAGGTCGGCCTCAGCA GAGCGCTATCCTCCACTGGTTACCCGGGTTCCCCGGCTTGCTACCGGGCTGCTCCGTGCA TCTTTCCCCCCAGGCGTCAGGAACTGCGCCCTCATGGCGGAGGTGAAGGTGAAGGTGCAG CCGCCTGACGCGGATCCGGTCGAAATAGAAAACAGGATTATAGAATTATGTCACCAGTTC CCTCATGGAATCACAGACCAAGTAATTCAGAATGAAATGCCTCATATAGAAGCCCAGCAG CGGGCAGTAGCCATCAATAGGTTGTTGTCTATGGGTCAGTTGGATCTCTTAAGGAGCAAT ACGGGCCTTTTATATAGAATAAAGGACTCTCAGAATGCTGGGTAAGTACTTGTTTTTTTA ATTTTAAGAAAATGTTTATTATTTGCAAACGTATGTGTTGGGAATAAAGCTAATACAAAA TTACATAAACCTGTCCTGTGTATCATCCCTGCCTCCTATGATATATTCCATTTCCCAGAG GTAACTGCTCTTAATAATTTAGTGTATATCCTTTCTTCTAGCTATATATAAATACACATA GATGATTATATATTTATATATAATCTTTCTCTTTTTTTTTAACANAAATGGAATCATGCT ATCCATTCTGTTTTGCAATTTGTTTTTTTCACTTACTGTATCTTGGACATCTTTTCATGT TGGTACACACAGCTCTGTCTCATTCTATTTAAGTAAATGAAGGGATCCGAAACCCAGAAA ACCAGTATATCAATCATAGAGATGCAGAAATAAAGCATATGCAGCAGAGATTCCGCTATA AAGTAATTTGCCTTAACAGAATCAACAAATTCTGAGGATCTGGAAAGTAAAGCTATCAAA CTGTAG >OriGene 3' read for NM_006466 unedited
GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG GGGTTATCAACAACAGAAATTTATTTCTCACAATTCTGGAGGCTGGGAAGTCCAAAATCA AAGGGCTTACAGATTTGTTTTCTGGGGAAGACCCACTTCCTGGTTCGTGGATGTTTGTCT TCTCCCTTTCACATGGCAAAAAGGGTGATGGAGCTCTCTGGGTCTCTTTATATAAAGGCA CTAATCCCATTCATGAACGCTGTTACTCTCCTGACCTATCACTTACCAAAAGCCCCACCT CCAAATACCATCACATTGGAAATGAGATTTCAACATTAAAATTCTCGGTGCACACAAACA TTCAGTCTACTGCAGACATTAAATAAAAATCTAGGGTAAATTCTAACAAAGAATACATTT GAGTTAAAGAGTGAAACATAAGACTTTAAGAAAGAAGGAACAGTGAAAATATTCTTTAAA ATGAAAATGATAGTATTTATGTACTGAAAATATGTCCTTGACAGTAGTCATTGGGTTTGA AGTAAGTATGGTAGCATAATCTCCTAAAAGAACATTTCTGATACAAAAAGGAATTAATAT TGTGCGGAAAAGGCATGTTCCACTCTCCCCCAGTCATACCCCAAGTTTGCACTCTTTGCT ATTCCTGCCTTTAACCACTTCTAATTTCTTTTCAATGAAACCACTGAGAAAAGTGGAAAC CAGAAATGGTGGTAGTGAACCAGATTTAAAAAAAACCTCCATGGAGAACTTATAAAAAAC TTTAAAAGACATTTTNTACCTCTACCATTTNATAAACCTGGGAAATACGGTATGTTGCAT CTTTTTTCTGACCATCAAAGTCTGAAGAAGTGCCTCCCGAGTTCAAGGGGTTATTATTGA AAAGAATTCCTACTACCTATTCTTATATTACAGGCTTTACCAATAAATACCTCTA |
Restriction Sites | NotI-NotI |
ACCN | NM_006466 |
Insert Size | 2870 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006466.2, NP_006457.2 |
RefSeq Size | 2177 bp |
RefSeq ORF | 951 bp |
Locus ID | 10621 |
UniProt ID | Q9H1D9 |
Domains | RNA_pol_Rpc34 |
Protein Families | Transcription Factors |
Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
Gene Summary | The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203100 | POLR3F (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F) |
CNY 2,400.00 |
|
RC203100L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203100L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), mGFP tagged |
CNY 5,890.00 |
|
RG203100 | POLR3F (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F) |
CNY 4,000.00 |