TXNRD2 (NM_006440) Human Untagged Clone

CAT#: SC116122

TXNRD2 (untagged)-Human thioredoxin reductase 2 (TXNRD2), nuclear gene encoding mitochondrial protein (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)



  "NM_006440" in other vectors (2)

CNY 7,832.00

CNY 8,460.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Rabbit polyclonal anti-TRXR2 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Synonyms GCCD5; SELZ; TR; TR-BETA; TR3; TRXR2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116122 sequence for NM_006440 edited (data generated by NextGen Sequencing)
ATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGGACGCAG
GCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGGGACTAT
GATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCCGCTCAG
CTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACCCGGTGG
GGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCACCAGGCG
GCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCCCAGCCC
GTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCCTTGAAC
TGGGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAAGCCAGC
TTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTGCTGTCA
GCCGATCACATCATCATTGCTACTGGAGGGCGGCCGAGATACCCCACGCACATCGAAGGT
GCCTTGGAATATGGAATCACAAGTGATGACATCTTCTGGCTGAAGGAATCCCCTGGAAAA
ACGTTGGTGGTCGGGGCCAGCTATGTGGCCCTGGAGTGTGCTGGCTTCCTCACCGGGATT
GGGCTGGACACCACCATCATGATGCGCAGCATCCCCCTCCGCGGCTTCGACCAGCAAATG
TCCTCCATGGTCATAGAGCACATGGCATCTCATGGCACCCGGTTCCTGAGGGGCTGTGCC
CCCTCGCGGGTCAGGAGGCTCCCTGATGGCCAGCTGCAGGTCACCTGGGAGGACAGCACC
ACCGGCAAGGAGGACACGGGCACCTTTGACACCGTCCTGTGGGCCATAGGTCGAGTCCCA
GACACCAGAAGTCTGAATTTGGAGAAGGCTGGGGTAGATACTAGCCCCGACACTCAGAAG
ATCCTGGTGGACTCCCGGGAAGCCACCTCTGTGCCCCACATCTACGCCATTGGTGACGTG
GTGGAGGGGCGGCCTGAGCTGACACCCACAGCGATCATGGCCGGGAGGCTCCTGGTGCAG
CGGCTCTTCGGCGGGTCCTCAGATCTGATGGACTACGACAATGTTCCCACGACCGTCTTC
ACCCCGCTGGAGTATGGCTGTGTGGGGCTGTCCGAGGAGGAGGCAGTGGCTCGCCACGGG
CAGGAGCATGTTGAGGTCTATCACGCCCATTATAAACCACTGGAGTTCACGGTGGCTGGA
CGAGATGCATCCCAGTGTTATGTAAAGATGGTGTGCCTGAGGGAGCCCCCACAGCTGGTG
CTGGGCCTGCATTTCCTTGGCCCCAACGCAGGCGAAGTTACTCAAGGATTTGCTCTGGGG
ATCAAGTGTGGGGCTTCCTATGCGCAGGTGATGCGGACCGTGGGTATCCATCCCACATGC
TCTGAGGAGGTAGTCAAGCTGCGCATCTCCAAGCGCTCAGGCCTGGACCCCACGGTGACA
GGCTGCTGAGGGTAA

Clone variation with respect to NM_006440.3
177 c=>t;196 g=>t;1109 t=>c
>OriGene 5' read for NM_006440 unedited
CCGGGCCCCCCCNNCCCNCCCCCCCCTCCCCCCCCCCCNCCCCCGTTCAAATTTGTNATA
CGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCAGCCCTAGCTGCCCCAGAAGCCCC
ACGACGATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGG
ACGCAGGCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGG
GACTATGATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCC
GCTCAGCTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACC
CGGTGGGGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCAC
CAGGCGGCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCC
CAGCCCGTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCC
TTGAACTGNGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAA
GCCAGCTTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTG
CTGTCAGCCGATCACATCATCATTGCTACTGGNAGGCGGCCGAGATACCCCACGCACATC
GAAGGTGCCTTGGAATATGGAAATCACAGTGATGACATCTTCTGGCTGAAGGAAATCCCT
GGNAANACGTNGNTGGGTCGGGCCAGCTATGTGGNCCTGGAGTGTGCTGGCTTTCTCACC
GGGATTGGGGCTGGACACACATCATGATGCGCAGCATCCCCCTTCGCGGNTTTCGACAGC
AAAGGTNCTCCATGGTCATAGAACACATGGCATCTCATGGCACCCGGNTCCTGAGGGGNC
TGGGGCCCCCTCCCGGGTCAGGAAGCCTTCCTGATGGC
>OriGene 3' read for NM_006440 unedited
TTTAAAACGGTCCTACTATGNACCGCGCCGCAATCTAAATCGAGTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTCACACTTACAAAAAAAGTACCCTCTTTATTTGCATTGCAAAAATGC
CAGGGGCGCTCGGACATGGGTGCCCACAGGGTAGGTTTCCACCCAGGTCGTCATCATCTG
GCACCAGGAGCCTGGGGGGGGGTGCCCGGCGGCAAAGGCTGAAAGGTGCAGGGGTGAGGT
GGACAGTCCCCTGAGCCATCCCTGTGGGGCACACGGGCCACCTGTCCAGCACTTGCGCTC
CACGCTGACCATCTCACAGGCGCTCCAGGAGAGGGTTGAGGCCCCCCAAACCTGGCCTGC
AGCCATCCTGGGTCTGGCCTCCGAGGAGCTGGCGGCGGGCGCACCGTGTGCCCTGGCCTG
CAGGGATGGCGCTTACCCTCAGCAGCCTGTCACCGTGGGGTCCAGGCCTGAGCGCTTGGA
GATGCGCAGCTTGACTACCTCCTCAGAGCATGTGGGATGGATACCCACGGTCCGCATCAC
CTGCGCATAGGAAGCCCCACACTTGATCCCCAGAGCAAATCCTTGAGTAACTTCGCCTGC
GTTGGGGCCAAGGAAATGCAGGCCCAGCACCAGCTGTGGGGGCTCCCTCAGGCACACCAT
CTTTACATAACACTGGGATGCATCTCGTCCAGCCACCGTGAACTCCAGTGGTTTATAATG
GGCGTGATAGACCTCAACATGCTCCTGCCCGTGGCGAGCCACTGCCCTCTTCTTCGACAG
CCCCACACAGCCTACTCCACGGGGTAGAAAACGGTCGGGGGAACATTGTCGTAGTCC
Restriction Sites NotI-NotI     
ACCN NM_006440
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006440.2, NP_006431.2
RefSeq Size 2180 bp
Locus ID 10587
UniProt ID Q9NNW7
Domains pyr_redox, pyr_redox_dim
Protein Families Druggable Genome
Protein Pathways Pyrimidine metabolism
Gene Summary The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017]
Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1, also known as hTR3a). This isoform is localized to the mitochondria (PMID:16774913). CCDS Note: This CCDS ID represents the protein described in PMIDs: 9923614 and 10215850. This transcript is supported by AF106697.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 9923614. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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