LASP1 (NM_006148) Human Untagged Clone
CAT#: SC116276
LASP1 (untagged)-Human LIM and SH3 protein 1 (LASP1)
CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Lasp-1; MLN50 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116276 sequence for NM_006148 edited (data generated by NextGen Sequencing)
ATGAACCCCAACTGCGCCCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGT CTGGATAAGTTCTGGCATAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAAC ATGAAGAACTACAAGGGCTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAG TCCTTCACCATGGTGGCGGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTC CAGAGTCAGGTGCGCTACAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTA GTGGCAGACACGCCCGAGCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATA AAATACCATGAGGAGTTTGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAG CCAGAGCGTCGGGATTCACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAG CCTCACCACATCCCGACCAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCC CAGTCCTATGGTGGCTACAAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCA GGTGGTGGCGGGAAGCGGTACCGCGTGGTGTATGACTACAGCGCCGCCGACGAGGACGAG GTCTCCTTCCAGGACGGGGACACCATCGTCAACGTGCAGCAGATCGACGACGGCTGGATG TACGGGACGGTGGAGCGCACCGGCGACACGGGGATGCTGCCGGCCAACTACGTGGAGGCC ATCTGA Clone variation with respect to NM_006148.2 626 c=>t >OriGene 5' read for NM_006148 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCAGCCGCCGTCGC TGCTGCCTGTGTAGTTGCAGCCGCGGCCGCCTCCCGCCAGCTCGCCTCGGGGAACAGGAC GCGCGTGAGCTCAGGCGTCCCCGCCCCAGCTTTTCTCGGAACCATGAACCCCAACTGCGC CCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGTCTGGATAAGTTCTGGCA TAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAACATGAAGAACTACAAGGG CTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAGTCCTTCACCATGGTGGC GGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTCCAGAGTCAGGTGCGCTA CAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTAGTGGCAGACACGCCCGA GCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATAAAATACCATGAGGAGTT TGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAGCCAGAGCGTCGGGATTC ACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAGCCTCACCACATCCCGAC CAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCCCAGTTCTATGGTGGGCT ACAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCAGGTGGGTGGCGGGAAAG CGTACCGGGTGGTGTATGACTACCAGCGCCGNCGACGAAGACGAGGTCTTCTTNCAGNAC GGGGACACCATCGNTCACGTGCANCAAATCNACNACGGTTGGATGTCCGGGACGGTGGAC N >OriGene 3' read for NM_006148 unedited
TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATATACTTTTATTTGAGTCACCAGGA GAAAGATTCACTTGTGGTTCACTTCAAACGTTCAGAATCATAACAGGCCAGAAAGGTTTG ATCCCGAGCACAAGCCCACGAGGGAGGGGACCAAAACAGACCAAAATGAGACAACAACCC CATATAAAAAGATGAACTGGCGGCTTCACACACTCACACACATACACATACACACGGATG AAATGTTTGGACAGAGGCAAATTTCACGTGGTCATTTCTGTTTCTTTTTAAATACATGTT TGTAGGGTGGTATTTTGTTTTTTCCAGCTATAAAAAAAGGCCCAAAAGTGCATGTGTGAG GGGGGAAAGGCANAAATTAAGCAATAAAGTCATTTTCCCTGGAGGGACATGAGAGGGAGA AAACAGGAGGCAGTGCTGGGAGAACGCACTCTCCTCACCACTGGGCTTCTTGTTATTCTT AGTATTGGTCCACAAAAGTTATATTCAACTTCTAGCTTTGATGCCTTCTTTCCTGAGATT AAATGAGCTGAAAGACCTCTGTGAACTGTAGAGAAGACCAGGGGCTTACCCCAACCCAGC CCAGCCCTCAAGCCTCTGTGCTCTCTAAGGATGAACGTTTTGGTAAAGACAGACGCCCAG CACCCCCCGGTACTAAGTACCATTGTGACAGGTCCTCCTCTCTCTGGGGTGGGAAAAAAA CGCCCCTATTTTAGACAGGACACGAAGGGAGGCCTCGCCCAGATCCCTGATGCTTTGCCT AATTGGAGGAAATTACCTGATGAAACACGCTGTTTTGTTGGCCTCCTTCACTCACATTTC CAACAAGGTCTTAAAGGGTGGGCCCGACCTGGCCCCAATCCACTGGCTTGAAACAAGGAA CAAAGCTACCGCCCCCAATCCAAAAACCTGCCCCCCACCCCCAAGCCATAAAGAATCCAT GCCCCATACTGCGCCAAGAAAGAAGCTACAAGCCCCCC |
Restriction Sites | NotI-NotI |
ACCN | NM_006148 |
Insert Size | 4310 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006148.1, NP_006139.1 |
RefSeq Size | 3846 bp |
RefSeq ORF | 786 bp |
Locus ID | 3927 |
UniProt ID | Q14847 |
Domains | NEBU, SH3, LIM |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A naturally monomeric infrared fluorescent protein for protein labeling in vivo
,Yu, D;Baird, MA;Allen, JR;Howe, ES;Klassen, MP;Reade, A;Makhijani, K;Song, Y;Liu, S;Murthy, Z;Zhang, SQ;Weiner, OD;Kornberg, TB;Jan, YN;Davidson, MW;Shu, X;,
Nat. Methods
,PubMed ID 26098020
[LASP1]
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Other Versions
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RC219975 | LASP1 (Myc-DDK-tagged)-Human LIM and SH3 protein 1 (LASP1) |
CNY 2,400.00 |
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RC219975L1 | Lenti ORF clone of Human LIM and SH3 protein 1 (LASP1), Myc-DDK-tagged |
CNY 4,800.00 |
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RC219975L2 | Lenti ORF clone of Human LIM and SH3 protein 1 (LASP1), mGFP tagged |
CNY 5,890.00 |
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RC219975L3 | Lenti ORF clone of Human LIM and SH3 protein 1 (LASP1), Myc-DDK-tagged |
CNY 4,800.00 |
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RC219975L4 | Lenti ORF clone of Human LIM and SH3 protein 1 (LASP1), mGFP tagged |
CNY 4,800.00 |
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RG219975 | LASP1 (tGFP-tagged) - Human LIM and SH3 protein 1 (LASP1) |
CNY 4,000.00 |