LASP1 (NM_006148) Human Untagged Clone

CAT#: SC116276

LASP1 (untagged)-Human LIM and SH3 protein 1 (LASP1)



  "NM_006148" in other vectors (6)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
LASP1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms Lasp-1; MLN50
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116276 sequence for NM_006148 edited (data generated by NextGen Sequencing)
ATGAACCCCAACTGCGCCCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGT
CTGGATAAGTTCTGGCATAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAAC
ATGAAGAACTACAAGGGCTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAG
TCCTTCACCATGGTGGCGGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTC
CAGAGTCAGGTGCGCTACAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTA
GTGGCAGACACGCCCGAGCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATA
AAATACCATGAGGAGTTTGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAG
CCAGAGCGTCGGGATTCACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAG
CCTCACCACATCCCGACCAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCC
CAGTCCTATGGTGGCTACAAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCA
GGTGGTGGCGGGAAGCGGTACCGCGTGGTGTATGACTACAGCGCCGCCGACGAGGACGAG
GTCTCCTTCCAGGACGGGGACACCATCGTCAACGTGCAGCAGATCGACGACGGCTGGATG
TACGGGACGGTGGAGCGCACCGGCGACACGGGGATGCTGCCGGCCAACTACGTGGAGGCC
ATCTGA

Clone variation with respect to NM_006148.2
626 c=>t
>OriGene 5' read for NM_006148 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCAGCCGCCGTCGC
TGCTGCCTGTGTAGTTGCAGCCGCGGCCGCCTCCCGCCAGCTCGCCTCGGGGAACAGGAC
GCGCGTGAGCTCAGGCGTCCCCGCCCCAGCTTTTCTCGGAACCATGAACCCCAACTGCGC
CCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGTCTGGATAAGTTCTGGCA
TAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAACATGAAGAACTACAAGGG
CTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAGTCCTTCACCATGGTGGC
GGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTCCAGAGTCAGGTGCGCTA
CAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTAGTGGCAGACACGCCCGA
GCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATAAAATACCATGAGGAGTT
TGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAGCCAGAGCGTCGGGATTC
ACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAGCCTCACCACATCCCGAC
CAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCCCAGTTCTATGGTGGGCT
ACAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCAGGTGGGTGGCGGGAAAG
CGTACCGGGTGGTGTATGACTACCAGCGCCGNCGACGAAGACGAGGTCTTCTTNCAGNAC
GGGGACACCATCGNTCACGTGCANCAAATCNACNACGGTTGGATGTCCGGGACGGTGGAC
N
>OriGene 3' read for NM_006148 unedited
TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATATACTTTTATTTGAGTCACCAGGA
GAAAGATTCACTTGTGGTTCACTTCAAACGTTCAGAATCATAACAGGCCAGAAAGGTTTG
ATCCCGAGCACAAGCCCACGAGGGAGGGGACCAAAACAGACCAAAATGAGACAACAACCC
CATATAAAAAGATGAACTGGCGGCTTCACACACTCACACACATACACATACACACGGATG
AAATGTTTGGACAGAGGCAAATTTCACGTGGTCATTTCTGTTTCTTTTTAAATACATGTT
TGTAGGGTGGTATTTTGTTTTTTCCAGCTATAAAAAAAGGCCCAAAAGTGCATGTGTGAG
GGGGGAAAGGCANAAATTAAGCAATAAAGTCATTTTCCCTGGAGGGACATGAGAGGGAGA
AAACAGGAGGCAGTGCTGGGAGAACGCACTCTCCTCACCACTGGGCTTCTTGTTATTCTT
AGTATTGGTCCACAAAAGTTATATTCAACTTCTAGCTTTGATGCCTTCTTTCCTGAGATT
AAATGAGCTGAAAGACCTCTGTGAACTGTAGAGAAGACCAGGGGCTTACCCCAACCCAGC
CCAGCCCTCAAGCCTCTGTGCTCTCTAAGGATGAACGTTTTGGTAAAGACAGACGCCCAG
CACCCCCCGGTACTAAGTACCATTGTGACAGGTCCTCCTCTCTCTGGGGTGGGAAAAAAA
CGCCCCTATTTTAGACAGGACACGAAGGGAGGCCTCGCCCAGATCCCTGATGCTTTGCCT
AATTGGAGGAAATTACCTGATGAAACACGCTGTTTTGTTGGCCTCCTTCACTCACATTTC
CAACAAGGTCTTAAAGGGTGGGCCCGACCTGGCCCCAATCCACTGGCTTGAAACAAGGAA
CAAAGCTACCGCCCCCAATCCAAAAACCTGCCCCCCACCCCCAAGCCATAAAGAATCCAT
GCCCCATACTGCGCCAAGAAAGAAGCTACAAGCCCCCC
Restriction Sites NotI-NotI     
ACCN NM_006148
Insert Size 4310 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006148.1, NP_006139.1
RefSeq Size 3846 bp
RefSeq ORF 786 bp
Locus ID 3927
UniProt ID Q14847
Domains NEBU, SH3, LIM
Protein Families Druggable Genome
Gene Summary This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]
Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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