NFYB (NM_006166) Human Untagged Clone

Name: NFYB (NM_006166) Human Untagged Clone
SKU: SC116285
Price: 2400.00

CAT＃: SC116285

NFYB (untagged)-Human nuclear transcription factor Y, beta (NFYB)

Datasheet

SDS

"NM_006166" in other vectors (6)

CNY 2,400.00

CNY 2,950.00

货期*

现货

规格

Cited in 2 publications.

Product images

经常一起买 (4)

TA324547

Rabbit polyclonal NFYB Antibody (N-term)

CNY 6,160.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CBF-A; CBF-B; HAP3; NF-YB
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_006166 edited GAATTCGGCACGAGGCCGCGGCGGAGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCG CGCTGGGACTGGTTCCTTCGCAGCCATTTTCTGTCCAACCAAACAGCCGATTGGAGACGG GAGCCAACCAGGGCTGCATTGGAGGTTGAAATCACAAAGATTAGACACCTTTTTAGATAG GTGTTCTTCAGCACCACTGACAACACGGTTCTGACAGTATTTCATGACAATGGATGGTGA CAGTTCTACAACAGATGCTTCTCAACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCA TTATGTTATACAGCCTCATGATGATACTGAGGACAGCATGAATGATCATGAAGACACAAA TGGTTCAAAAGAAAGTTTCAGAGAACAAGATATATATCTTCCAATAGCAAACGTGGCTAG GATAATGAAAAATGCCATACCTCAAACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGT TCAAGAATGTGTAAGTGAGTTCATCAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCA TCAAGAGAAACGGAAAACAATCAATGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGG CTTTGACAGTTATGTGGAACCTCTGAAATTATACCTTCAGAAATTCAGAGAGGCTATGAA AGGAGAAAAGGGAATTGGTGGAGCAGTCACAGCTACAGATGGACTAAGTGAAGAGCTTAC AGAGGAGGCATTTACTAACCAGTTACCAGCTGGCTTAATAACCACAGACGGTCAACAACA AAATGTTATGGTTTACACAACATCATATCAACAGATTTCTGGTGTTCAGCAAATTCAGTT TTCATGATCTGAAGAAATGATGGAATGGGGAGTGTAGAGAAATGAGAGTCTGTATGATTC TGGAACAGAGACATCAGAAGGAAAGACTGGTGAAAAGATGTATCTTTGTATATTAATAGC TGTAATGTAGCTTCCTGATGCTTGACTAATTGAGGTGTTAATTCTGACTTGAGAATCTTT TTCATGAATGATTTTAAAGAAAAATTTGGATTTTAAAGGTATTAAAATATTTTTGTTTTG TACGAGAGTTTGTTGCTCTGTATGACTCCTGTATGCATTGTATATTGCAATTTATTACTG TCAGAGATTTGTAGACAGTTTCTTATTTTCATATTGAATCATGTTACTTTTGTAATTCAA GTAAGCGGCTGGGTTAATTCATGATGTTTGCCCTTTTAATAAAATATAAGGGTAGAGTTC ATTTTGAATGCAAGTTGCCTTTATTATAAATTTGAGTTTGTCTTGGTTATACCTTGCATG ATAACCTAGCTAGATTTCTAGCATTTGCTGTATTTATTAAAATTATTATTTTTTTGGTAA AACATTAATAGTTTAAGCAGCATCATTTTTTTAAAAAATGTAATTGAATAAGTGTGAATG CAGAAGCAAATATTGTCTGCCCTGTTAAACTTGGTGCCCATTAACAGTGTTTACACTGTT CATCGTGCCTGTTAATGTAGTTTTAGTTACTGGAGCTTTTTTAAGACTAGATTTGGTTTT GAGTTACATTTTTAAGAATGTGGGAATATATTTAAGTTTAATGTAGTCCTAGTGCTCTTG AAATGGTGCCCCTTTCATTTGGTACATGATTTTTTTTCAAATCATATCTTCAAGTACTAT AGTATTCTCTTACAGAAGAGGAGTTTTATAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XGTCTTCATTTTACCTTTTTAATTGAAATGTCAAGTTTCCTGTTACACTATGGAAACCAA GAAACATCAGACATCATTGCGTGTACAGACCTTTTGCATGGGTGAGTGGATGAAATGGAG AACAGAGTGAGTGCTGTGAACGGTGTGAAATAGAAGCCAACTTCTAGTATGCTGTCTTCA TCTCTGCAATAAACTAAACGTAAATAATGTAACTTTGTATAATTTAGTAGTATGTACTTG GATTGTTTCTTTTTGAAAAGTGAGGATTTGTATTTTCTCATCTATTCTTTTTGGTGTTAA TTAACTTGAGGGATTTCTTTTTTAAGTTTTTAATATCAAAAATTGCTATTGTATAGTTTG CCTAAAAGGAAGTTGGAGTGAATATCATCTTTGAAGGACACTCAAATTTATAAGGTAGAA GCAATTTCCCAGATATAGGCTGAACAGAAACATTTACTTTGCCAGAAGGAGCCTTTAGGA GTATATGTGGGCTAATCAGAGGTTAATAATGTCCTCATCTCCTACTCTCCATTTAAAAAA CAAACTTCATTGTAGTAAATTCAAATAGGAAAAAATGGGGGAGTAGTTAAGCAAAATCAA CAGTCATCCTACTATGTTGAAAGAACCAATGTTAATATTTTGATAAATATCATTTGTCCT TTTACTTTGCTAAATATGTATATTTTTATAAAAATGGGCTCATATTGTGCATACCATTTT GTAACCTGCTTTTTTCACTTAACAATATATTGGGAACATCTTTAAAAAAAAAAAAAAAAA AAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006166 unedited AGGTCGAATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCCGCGGCGG AGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCGCGCTGGGACTGGTTCCTTCGCAGC CATTTTCTGTCCAACCAAACAGCCGATTGGAGACGGGAGCCAACCAGGGCTGCATTGGAG GTTGAAATCACAAAGATTAGACACCTTTTTAGATAGGTGTTCTTCAGCACCACTGACAAC ACGGTTCTGACAGTATTTCATGACAATGGATGGTGACAGTTCTACAACAGATGCTTCTCA ACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCATTATGTTATACAGCCTCATGATGA TACTGAGGACAGCATGAATGATCATGAAGACACAAATGGTTCAAAAGAAAGTTTCAGAGA ACAAGATATATATCTTCCAATAGCAAACGTGGCTAGGATAATGAAAAATGCCATACCTCA AACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGTTCAAGAATGTGTAAGTGAGTTCAT CAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCATCAAGAGAAACGGAAAACAATCAA TGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGGCTTTGACAGTTATGTGGAACCTCT GAAATTATACCTTCAGAAATTCAGAGAGGCTATGAAAGGAGAAAAGGGAATTGGGGGAGC AGTCACAGCTACAGATGGACTAAGTGAAGAGCTTACAGAGGAGGCATTTACTAACCAGTT ACCAGCTGGCTTAATAACCACAGACGGTCACAACAAATGTTAATGGTTTACACAACATCA TATAACAGATTTTTTNTGTTCACCANATTCAGTTTCATGATCTGAACAATGATGAAAGGG GGGAGTGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_006166 unedited ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAAATGTTCCCAA TATATTGTTAAGTGAAAAAAGCGGTTACAAAATGGTATGCACAATATGAGCCCATTTTTA TAAAAATATACATATTTAGCAAAGTAAAAGGACAAATGATATTTATCAAAATATTAACAT TGGTTCTTTCAACATAGTAGGATGACTGTTGATTTTGCTTAACTACTCCCCCATTTTTTC CTATTTGAATTTACTACAATGAAGTTTGTTTTTTAAATGGAGAGTAGGAGATGAGGACAT TATTAACCTCTGATTAGCCCACATATACTCCTAAAGGCTCCTTCTGGCAAAGTAAATGTT TCTGTTCAGCCTATATCTGGGAAATTGCTTCTACCTTATAAATTTGAGTGTCCTTCAAAG ATGATATTCACTCCAACTTCCTTTTAGGCAAACTATACAATAGCAATTTTTGATATTAAA AACTTAAAAAAGAAATCCCTCAAGTTAATTAACACCAAAAAGAATAGATGAGAAAATACA AATCCTCACTTTTCAAAAAGAAACAATCCAAGTACATACTACTAAATTATACAAAGTTAC ATTATTTACGTTTAGTTTATTGCAGAGATGAAGACAGCATACTAGAAGTTGGCTTCTATT TCACACCGTTCACAGCACTCACTCTGTTCTCCATTTCATCCACTCACCCATGCAAAAGGT CTGTACACCCAATGATGCCTGATGGTTCTTGCGTTCCATAGCGCAACAAGAAAACTCGAC ATTTCAATTATAAAGGGTAAATGAAGACCTTAACCATCANACTATTAAACTGCTCTTNTG TAAGAGGATCCTATATTACTTGAAAATTTAATTGAAAAAAAATCATGTCCCAAATGAAGG GGCCCCTTTCAGAGCCTAGGACTACCTTAACTTAATTATT
Restriction Sites	NotI-NotI
ACCN	NM_006166
Insert Size	2850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006166.3, NP_006157.1
RefSeq Size	3482 bp
RefSeq ORF	624 bp
Locus ID	4801
UniProt ID	P25208
Domains	CBFD_NFYB_HMF
Protein Families	Transcription Factors
Protein Pathways	Antigen processing and presentation
Gene Summary	The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
Regulation of the p53 transcriptional response by structurally diverse core promoters ,null, Genes & Development ,PubMed ID 20040571 [NFYB]
Regulation of the p53 transcriptional response by structurally diverse core promoters ,José M. Morachis, Christopher M. Murawsky, and Beverly M. Emerson, Genes & Dev., Jan 2010; 24: 135 - 147 [NFYB]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202749	NFYB (Myc-DDK-tagged)-Human nuclear transcription factor Y, beta (NFYB)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,705.00
RC202749L1	Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC202749L2	Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202749L3	Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202749L4	Lenti ORF clone of Human nuclear transcription factor Y, beta (NFYB), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG202749	NFYB (tGFP-tagged) - Human nuclear transcription factor Y, beta (NFYB)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00

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Proteins

Antibodies