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NSF (NM_006178) Human Untagged Clone
CAT＃: SC116292
NSF (untagged)-Human N-ethylmaleimide-sensitive factor (NSF)
CNY 5,456.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SEC18; SKD2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116292 sequence for NM_006178 edited (data generated by NextGen Sequencing)
ATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGATGAATTATCTTTAACCAAT TGTGCAGTTGTGAATGAAAAGGATTTCCAGTCTGGCCAGCATGTGATTGTGAGGACCTCT CCCAATCACAGGTACACATTTACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATT GCATTCAGTTTACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAGAAGTC TCCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATCGAGATTGAT TTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACACCGACAAGATGGCAGCAGAA TTTATTCAGCAATTCAACAACCAGGCCTTCTCAGTGGGACAACAGCTTGTCTTTAGCTTC AATGAAAAGCTTTTTGGCTTACTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTG AAGGGAGAGCCTGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAAC AGTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGCAAAGCTAAA ACCAAGGAAAATCGCCAATCAATTATCAATCCTGACTGGAACTTTGAAAAAATGGGAATA GGAGGTCTAGACAAGGAATTTTCAGATATTTTCCGACGAGCATTTGCTTCCCGAGTATTT CCTCCAGAGATTGTGGAGCAGATGGGTTGTAAACATGTTAAAGGCATCCTGTTATATGGA CCCCCAGGTTGTGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGA GAGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAATCAGAGGCT AACATTCGCAAACTTTTTGCTGATGCTGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGT GGTTTGCACATCATCATCTTTGATGAAATTGATGCCATCTGCAAGCAGAGAGGGAGCATG GCTGGTAGCACGGGAGTTCATGACACTGTTGTCAACCAGTTGCTGTCCAAAATTGATGGC GTGGAGCAGCTAAACAACATCCTAGTCATTGGAATGACCAATAGACCAGATCTGATAGAT GAGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTTGCCAGATGAG AAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAATGAGAGGGCATCAGTTACTC TCTGCTGATGTAGACATTAAAGAACTGGCCGTGGAGACCAAGAATTTCAGTGGTGCTGAA TTGGAGGGTCTGGTGCGAGCAGCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGT ACTAAAGTGGAAGTGGACATGGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTC CTTGCTTCTTTGGAGAATGATATCAAACCAGCCTTTGGCACAAACCAAGAAGATTATGCA AGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAGTTACTCGAGTTCTAGATGAT GGGGAGCTGCTGGTGCAGCAGACTAAGAACAGTGACCGCACACCATTGGTCAGCGTGCTT CTGGAAGGCCCTCCTCACAGTGGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCC AACTTCCCGTTCATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCC AAATGTCAGGCCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTCAGTTGTGTG GTTGTGGATGACATTGAGAGATTGCTTGATTACGTCCCTATTGGCCCTCGATTTTCAAAT CTTGTATTACAGGCTCTTCTCGTTTTACTGAAAAAGGCACCTCCTCAGGGCCGCAAGCTT CTTATCATTGGGACCACTAGCCGCAAAGATGTCCTTCAGGAGATGGAAATGCTTAACGCT TTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTTGGAAGCTTTG GAGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAAGTCAAAGGG AAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGATCGAGATGTCCCTACAGATG GATCCTGAATACCGTGTGAGAAAATTCTTGGCCCTCTTAAGAGAAGAAGGAGCTAGCCCC CTTGATTTTGATTGA Clone variation with respect to NM_006178.3 >OriGene 5' read for NM_006178 unedited
TCTTTTTGTATACGACTTATCTATAGGGCGGCCGCGAATTCGCACGAGGCGGACGTGTCC GCGAAGAGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGATGAATTATCTTTAA CCAATTGTGCAGTTGTGAATGAAAAGGATTTCCAGTCTGGCCAGCATGTGATTGTGAGGA CCTCTCCCAATCACAGGTACACATTTACACTGAAGACACATCCATCGGTGGTTCCAGGGA GCATTGCATTCAGTTTACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAG AAGTCTCCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATCGAGA TTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACACCGACAAGATGGCAG CAGAATTTATTCAGCAATTCAACAACCAGGCCTTCTCAGTGGGACAACAGCTTGTCTTTA GCTTCAATGAAAAGCTTTTTGGCTTACTGGTGAAGGACATTGAAGCCATGGATCCTAGCA TCCTGAAGGGAGAGCCTGCGACAGGGAAAAGGCAGAAGATTGAAGTACGACTGGTTGTTG GAAACAGTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGCAAAG CTAAACCCAGGAAAATCGCCCATCAATTATCAATCCTGACTGGAACTTTTGAAAAAGGGA ATAGGAGGTCTAGACCAGGAATTTTAGATATCTTCCGACAAGCATTTGCTTTCCGAGTAT TTCCTTCCAAAATTGTGGAGCAGAAGGGGTTGTACACATGGTAACAGGCATCCTGTTTTT TGGACCCCCCAGCTGGCGGCAAGACTCTCTTGCTTCCACCAATTGGCCAGAGTTGAATGC AAGAAAGCCCAAGTGGTCATGGGGCTCAAATTCTTAACCAAATT >OriGene 3' read for NM_006178 unedited
TTTTTTTCAAGTATATTTCNACTCNCTNTNTNNATNTGGGCATTNCCTTCATTTGCATTA AACAATATTTTTTCAATACAGTTTTGGACAAAACACAAAGACATTAAGCCTCATTTTAAC AAGAGACATAAGTTAACACAATGTGTGCTGCTTTTCATGAGGAGGAAAGAGGCAANNNNN NNATNNCCTTTAGAGGAATTCCAGGATACTGGCCACNCCCNNANNNGAATCACAGGATCN CTTTNNNNNNNAATACAATCNCCCCAATTTTTTTCNCCCCTTTTTAANNAAAGACCCCAA AATAAGCTAAGGAAGAAAACCCAAACAAAGAAGAATTGACCTCCAAGTCTCCCCAAAAGT ATACAAATGGCAAGATTTGGAGATGATCTGCTTTCTCACATGAGGACAAAATACAGAGGA GCCCCACCCAAGTGCCACTGTGGCCACAAGCCTCATGGGTGGCGTGTGAGGTAAGCACCT TNAGATGGCTTCTAATTGCTTGTCCTTGCATTTTAAGCAGCAGTTGGGAGAGAGAGCACA CTCCATAAGTCTCATCACCAAATATGACCTAATTAAATTCCTGCTAAGTCAGTCAGTTGT AAGACAAATCACCCTCAAAAGGACTCAACGGGTGCTTCCTTTTGGAAGAAGTGTGTGNAG GGTAAAGAAATGTGTTCAATGTATTGGAATGGACAATAAAGGACTGAAGGAGAAGCCTAC AAAATGATTGATTTTTTCTGGAAATTAATGTTTTAAACCCTAGATAGTCCAGATCATTTA AAATAAAGGATAATTCTCAATAATCTTTAACCTAGGAAAAAACCGTGGTTCTAGTTAGGC CTAATTTTTTCCTTAGGAATGCCCCCTTTGAATGGGCACCTATTAGGCTGGGGAGTTGGA AATACTTTAAAACCCTTTTTTTTGGGGGGGATGCCTTCCCTCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006178 |
| Insert Size | 4260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006178.1, NP_006169.1 |
| RefSeq Size | 3960 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 4905 |
| UniProt ID | P46459 |
| Domains | cdc48_N, AAA, AAA, cdc48_2 |
| Protein Families | Protease |
| Gene Summary | Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205983 | NSF (Myc-DDK-tagged)-Human N-ethylmaleimide-sensitive factor (NSF) |
CNY 5,448.00 |
|
| RC205983L1 | Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), Myc-DDK-tagged |
CNY 7,848.00 |
|
| RC205983L2 | Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), mGFP tagged |
CNY 5,890.00 |
|
| RC205983L3 | Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), Myc-DDK-tagged |
CNY 7,848.00 |
|
| RC205983L4 | Lenti ORF clone of Human N-ethylmaleimide-sensitive factor (NSF), mGFP tagged |
CNY 7,848.00 |
|
| RG205983 | NSF (tGFP-tagged) - Human N-ethylmaleimide-sensitive factor (NSF) |
CNY 7,048.00 |
