MANBA (NM_005908) Human Untagged Clone

CAT#: SC116441

MANBA (untagged)-Human mannosidase, beta A, lysosomal (MANBA)



  "NM_005908" in other vectors (4)

CNY 6,448.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
MANBA Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MANB1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116441 sequence for NM_005908 edited (data generated by NextGen Sequencing)
ATGCGCCTCCACCTGCTCCTGCTGCTCGCGCTGTGCGGTGCAGGCACCACCGCCGCGGAG
CTCAGTTACAGCTTGCGTGGCAACTGGAGCATCTGCAATGGGAACGGCTCGCTGGAGCTG
CCCGGGGCGGTCCCTGGCTGCGTGCACAGCGCCTTGTTCCAGCAGGGCCTGATCCAGGAT
TCTTACTACAGATTTAATGACCTTAACTACAGATGGGTCTCTTTGGATAACTGGACCTAT
AGCAAAGAATTTAAAATCCCCTTTGAAATTAGCAAATGGCAAAAAGTAAATTTGATTCTT
GAGGGAGTGGATACGGTTTCAAAAATCCTGTTCAATGAAGTCACTATTGGGGAAACAGAC
AATATGTTCAATAGATATAGCTTTGATATTACCAACGTGGTCAGGGACGTGAACTCCATT
GAGCTGCGTTTCCAGTCAGCGGTGTTGTATGCAGCACAGCAGAGCAAAGCTCACACTCGC
TACCAGGTTCCCCCAGACTGCCCTCCACTTGTGCAGAAGGGTGAATGCCATGTCAACTTT
GTTCGGAAGGAGCAATGTTCCTTTAGTTGGGACTGGGGGCCTTCCTTTCCTACCCAGGGA
ATCTGGAAAGATGTTAGAATTGAAGCCTATAATATTTGTCACCTGAACTACTTCACATTT
TCCCCAATATATGATAAGAGTGCCCAGGAGTGGAATCTGGAAATAGAGTCTACATTTGAT
GTTGTCAGCTCAAAGCCAGTTGGTGGTCAAGTGATCATAGCCATCCCTAAGTTGCAAACA
CAACAGACATACAGCATTGAACTTCAACCTGGGAAAAGGATTGTTGAGCTATTTGTGAAC
ATTAGCAAGAATATTACTGTAGAAACTTGGTGGCCTCATGGACATGGAAACCAGACTGGG
TACAACATGACTGTTCTTTTTGAACTGGATGGAGGCTTAAATATTGAAAAATCAGCTAAG
GTTTATTTTAGGACAGTGGAACTTATAGAAGAGCCTATAAAAGGGTCTCCTGGTTTGAGT
TTCTATTTCAAAATTAATGGATTTCCCATATTTCTAAAAGGCTCAAACTGGATCCCAGCA
GATTCATTCCAGGACCGAGTAACCTCTGAGTTGTTACGGCTCCTTTTACAGTCTGTTGTG
GATGCTAATATGAATACTCTTCGGGTTTGGGGAGGAGGAATTTATGAGCAGGATGAATTC
TATGAACTCTGTGATGAACTAGGAATAATGGTATGGCAGGATTTTATGTTTGCCTGTGCC
CTTTATCCAACTGATCAGGGCTTCCTGGATTCAGTGACAGCAGAAGTTGCCTACCAGATC
AAGAGACTGAAATCTCATCCTTCTATCATCATATGGAGTGGCAATAATGAAAATGAGGAG
GCGCTGATGATGAATTGGTATCATATCAGTTTCACTGACCGGCCAATCTACATCAAGGAC
TATGTGACACTCTATGTGAAAAACATCAGAGAGCTCGTACTGGCAGGAGACAAGAGTCGT
CCTTTTATTACGTCCAGTCCTACAAATGGGGCTGAAACTGTTGCAGAAGCCTGGGTCTCT
CAAAACCCTAATAGCAATTATTTTGGTGATGTACATTTTTATGACTATATCAGTGATTGC
TGGAACTGGAAAGTTTTCCCAAAAGCTCGATTTGCATCTGAATATGGATATCAGTCCTGG
CCGTCCTTCAGTACATTAGAAAAGGTCTCGTCTACAGAGGACTGGTCTTTCAATAGCAAG
TTTTCACTTCATCGACAACATCACGAAGGTGGTAACAAACAAATGCTTTATCAGGCTGGA
CTTCATTTCAAACTCCCCCAAAGCACAGATCCATTACGCACATTTAAAGATACCATCTAC
CTTACTCAGGTGATGCAGGCCCAGTGTGTCAAAACAGAAACTGAATTCTACCGCCGTAGT
CGCAGCGAGATAGTGGATCAGCAAGGGCACACGATGGGGGCACTTTATTGGCAGTTGAAT
GACATCTGGCAAGCTCCTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTGGAAAATGCTT
CATTACTTTGCTCAGAATTTCTTTGCTCCACTGTTGCCAGTAGGCTTTGAGAATGAAAAC
ACGTTCTATATCTATGGTGTGTCAGATCTTCACTCGGATTATTCGATGATACTCAGTGTG
AGAGTCCATACATGGAGCTCCCTGGAGCCCGTGTGCTCTCGTGTGACTGAACGTTTTGTG
ATGAAAGGAGGAGAGGCTGTCTGCCTTTATGAGGAGCCAGTGTCTGAATTGCTGAGGAGA
TGTGGGAATTGCACACGGGAAAGCTGTGTGGTTTCCTTTTACCTTTCAGCTGACCATGAA
CTCCTGAGCCCGACCAACTACCACTTCTTGTCCTCACCGAAGGAGGCCGTGGGGCTCTGC
AAGGCGCAGATCACTGCCATCATCTCTCAGCAAGGTGACATATTTGTTTTTGACCTGGAG
ACCTCAGCTGTCGCTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCAGGGAGATTTAGT
GACAATGGTTTCCTCATGACTGAGAAGACACGAACTATATTATTTTACCCTTGGGAGCCC
ACCAGCAAGAATGAGTTGGAGCAATCTTTTCATGTGACCTCCTTAACAGATATTTACTGA

Clone variation with respect to NM_005908.3
757 g=>a;2150 c=>t
>OriGene 5' read for NM_005908 unedited
GTTAGGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCACATCT
CGGTGGCGCGGGATCTCAAGAGCGCCTCCACCTGCTCCTGCTGCTCGCGCTGTGCGGTGC
AGGCACCACCGCCGCGGAGCTCAGTTACAGCTTGCGTGGCAACTGGAGCATCTGCAATGG
GAACGGCTCGCTGGAGCTGCCCGGGGCGGTCCCTGGCTGCGTGCACAGCGCCTTGTTCCA
GCAGGGCCTGATCCAGGATTCTTACTACAGATTTAATGACCTTAACTACAGATGGGTCTC
TTTGGATAACTGGACCTATAGCAAAGAATTTAAAATCCCCTTTGAAATTAGCAAATGGCA
AAAAGTAAATTTGATTCTTGAGGGAGTGGATACGGTTTCAAAAATCCTGTTCAATGAAGT
CACTATTGGGGAAACAGACAATATGTTCAATAGATATAGCTTTGATATTACCAACGTGGT
CAGGGACGTGAACTCCATTGAGCTGCGTTTCCAGTCAGCGGTGTTGTATGCAGCACAGCA
GAGCAAAGCTCACACTCGCTACCAGGTTCCCCCAGACTGCCCTCCACTTGTGCAGAAGGG
TGAATGCCATGTCAACTTTGTTCGGAAGGAGCAATGTTCCTTTAGTTGGGACTGGGGGCC
TTCCTTTCCTACCCAGGGAATCTGGAAAGATGTTAGAATTGAAGCCTATAATATTTGTCA
CCTGAACTACTTCACATTNTCCCCATATATGATAAGAGTGCCCAGGAGTGGAATCTGGAA
ATAGAGTCTACATTCTGATGTGTCAGCTCAAGCCAGTTGGTGGTCAAGTGATCATAGCCA
TNCCCTAGTTGCAAACACACAGACATACAGCATTGAACTTCAACCTGGAAAAAGGATGTT
GAGCCTATTGTGAACACTAGCAGAATATT
>OriGene 3' read for NM_005908 unedited
ATGGACCGAGGCTCCCCTTCTACAACGAGTTTTTCTTTTTTTTTTTTTTTTTTTACAAGG
TATTTTATATAACTTTTTTATTTTTATATACACTTTCACAGTGGACAGAAAAAATACCCA
TATACCTTTACCCAAATAGCTACCACCAAAAAATCTCTGTGGAAACATTTCAATCGCCAT
TCCCTCTGAAATAATAACAAAGCACTTTATTTGAGTATTCCGTATTCCCCAAAGCTAGCT
GGGAAAGACCTACATCACCTCAAAACCTTCCATTGGGTTCCATAGATCCTGCCATGTCAC
ATTCTTGTAACCAGCTGCAGGGCCATCTTGTTATAACTGGTTCATGTCCTGCTAAAGCTG
AAAGGGGAAGGGCTAAAAACACAGTCCTGGCACAGATTCCAGGACACCCCGGCACAGGCT
TGTTTGAGGACATTGCCTGGGTAAACAGCCTCCCCTGAAAGTGTTCAGGGTACAACTCTT
CACAGAGCAATCGTTCAAATGCGGGGCAGCACGCAGACATGTCTCTCGGCTTCTCTCCTT
GACTCTGTGGCCTTCCTCTCCAGTCGGTGCTTTAAAAATGCTTTATTCCCATGGTCCACT
GAAAATACAACCTAAATTCCTTCAGTAAATATCTGTCAAGGAGGCCACATGAAAAGATTG
CTCCAACTCATTTCTGCTGGATGGCCTCCAAGGGTAAAAAATATAATTCGTGTCTTCTCA
GCCATGAAGGAACCCTTGCCCCTACATCTCCCTGGGATGCTTCCTACTTCAACCAAACAA
AGTAGCGACCGTTGAAGTCTCCAGGTCAAAAAAAATAGTCACCTTGCTGAAATAGATGCA
CTGATCGGCGCTTGAAAACCCCAGGTCTCCTCCGGGAGGACACAAGGGAATTGGCGGCCT
CAGATTTATGTCACCTGAAGGAAAGGAACCCCCATTTTCCGGGGAATCCAACTTCTAAAA
TAAAACATCCTCCTAAAGGGAAAGCTTC
Restriction Sites NotI-NotI     
ACCN NM_005908
Insert Size 3160 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005908.2, NP_005899.2
RefSeq Size 3308 bp
RefSeq ORF 2640 bp
Locus ID 4126
UniProt ID O00462
Domains Glyco_hydro_2_C
Protein Pathways Lysosome, Other glycan degradation
Gene Summary This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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