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SnoN (SKIL) (NM_005414) Human Untagged Clone
CAT＃: SC116749
SKIL (untagged)-Human SKI-like oncogene (SKIL), transcript variant 1
CNY 5,016.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SNO; SnoA; SnoI; SnoN |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005414, the custom clone sequence may differ by one or more nucleotides
ATGGAAAACCTCCAGACAAATTTCTCCTTGGTTCAGGGCTCAACTAAAAAACTGAATGGGATGGGAGATG ATGGCAGCCCCCCAGCGAAAAAAATGATAACGGACATTCATGCAAATGGAAAAACGATAAACAAGGTGCC AACAGTTAAGAAGGAACACTTGGATGACTATGGAGAAGCACCAGTGGAAACTGATGGAGAGCATGTTAAG CGAACCTGTACTTCTGTTCCTGAAACTTTGCATTTAAATCCCAGTTTGAAACACACATTGGCACAATTCC ATTTAAGTAGTCAGAGCTCGCTGGGTGGACCAGCAGCATTTTCTGCTCGGCATTCCCAAGAAAGCATGTC GCCTACTGTATTTCTGCCTCTTCCATCACCTCAGGTTCTTCCTGGCCCATTGCTCATCCCTTCAGATAGC TCCACAGAACTCACTCAGACTGTGTTGGAAGGGGAATCTATTTCTTGTTTTCAAGTTGGAGGAGAAAAGA GACTCTGTTTGCCCCAAGTCTTAAATTCTGTTCTCCGAGAATTTACACTCCAGCAAATAAATACAGTGTG TGATGAACTGTACATATATTGTTCAAGGTGTACTTCAGACCAGCTTCATATCTTAAAGGTACTGGGCATA CTTCCATTCAATGCCCCATCCTGTGGGCTGATTACATTAACTGATGCACAAAGATTATGTAATGCTTTAT TGCGGCCACGAACTTTTCCTCAAAATGGTAGCGTACTTCCTGCTAAAAGCTCATTGGCCCAGTTAAAGGA AACTGGCAGTGCCTTTGAAGTGGAGCATGAATGCCTAGGCAAATGTCAGGGTTTATTTGCACCCCAGTTT TATGTTCAGCCTGATGCTCCGTGTATTCAATGTCTGGAGTGTTGTGGAATGTTTGCACCCCAGACGTTTG TGATGCATTCTCACAGATCACCTGACAAAAGAACTTGCCACTGGGGCTTTGAATCAGCTAAATGGCATTG CTATCTTCATGTGAACCAAAAATACTTAGGAACACCTGAAGAAAAGAAACTGAAGATAATTTTAGAAGAA ATGAAGGAGAAGTTTAGCATGAGAAGTGGAAAGAGAAATCAATCCAAGACAGATGCACCATCAGGAATGG AATTACAGTCATGGTATCCTGTTATAAAGCAGGAAGGTGACCATGTTTCTCAGACACATTCATTTTTACA CCCCAGCTACTACTTATACATGTGTGATAAAGTGGTTGCCCCAAATGTGTCACTTACTTCTGCTGTATCC CAGTCTAAAGAGCTCACAAAGACAGAGGCAAGTAAGTCCATATCAAGACAGTCAGAGAAGGCTCACAGTA GTGGTAAACTTCAAAAAACAGTGTCTTATCCAGATGTCTCACTTGAGGAACAGGAGAAAATGGATTTAAA AACAAGTAGAGAATTATGTAGCCGTTTAGATGCATCAATCTCAAATAATTCTACAAGTAAAAGGAAATCT GAGTCTGCCACTTGCAACTTAGTCAGAGACATAAACAAAGTGGGAATTGGCCTTGTTGCTGCCGCTTCAT CTCCGCTTCTTGTGAAAGATGTCATTTGTGAGGATGATAAGGGAAAAATCATGGAAGAAGTAATGAGAAC TTATTTAAAACAACAGGAAAAACTAAACTTGATTTTGCAAAAGAAGCAACAACTTCAGATGGAAGTAAAA ATGTTGAGTAGTTCAAAATCTATGAAGGAACTCACTGAAGAACAGCAGAATTTACAGAAAGAGCTTGAAT CTTTGCAGAATGAACATGCTCAAAGAATGGAAGAATTTTATGTTGAACAGAAAGACTTAGAGAAAAAATT GGAGCAGATAATGAAGCAAAAATGTACCTGTGACTCAAATTTAGAAAAAGACAAAGAGGCTGAATATGCA GGACAGTTGGCAGAACTGAGGCAGAGATTGGACCATGCTGAGGCCGATAGGCAAGAACTCCAAGATGAAC TCAGACAGGAACGGGAAGCAAGACAGAAGTTAGAGATGATGATAAAAGAGCTAAAGCTGCAAATTCTGAA ATCATCAAAGACTGCTAAAGAATAG >OriGene 5' read for NM_005414 unedited
NGTTCAAAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGTGAGACAGAA AATGCTTGAACCCGGGAGGCGGAGGTTGCAGTATGCCGAGATCGCTGCACTTCAGCCTGG GCGACAGAGCGAGACTCCGTAAAAAAAAGAAAAAAAAAAAAAATAAAAGAAACTGGTTTC AAATTGGCCCTTTGGCCTCTGGAGCAAATTCAAATGTAACTCTTCCCCAACCCCCCTTCT CTTCTTCCAGATTAATTAAAAGAAGAATGAACTATAATCCTTGAAGATAACTGGGCAATT TTTTAAGTCGGAGGCTGTTCTTACTGGTGTGAGGATTTACACACGTCTTCAGTTTTTCAG CACAGACCAGCAGACCATCATTTTTAGAGGAAATACTCCCTCTGCCCTCCTTTTTGGTTT CCTTGGTGGTAAAGATTAAATTTGGTTGCATCATTTTGACTTGTGTTTGAGTCTAGGTTT TATGGCACAAGGAATGGCATAAACTTTTCATGTGTTTTGGTTAAAACAAACCAGACCATT GCATTGACCCTGGACATCTTTAATTGAGAAATTGGTAACTTTATTTTAATATGTATATCT GAAGAATTCAAGAAAACAAAGGCATCCTCAGAGGTGTGCCTCTTTTCTTTATTATTAGAG GCAAAACGAACAATTTTATAGGATTTGTAGTGAAATTATACCAGATTATAAGGAGAACCA AAACTAAGTCGCAAAATTTATTAATTTAAGGGGCTCTCGCTTTGAAAGTTTGAGAGTAAG TTACGATAGGCATTTGTATCCATTCATTACTTTCCTCTTTTCAAATAGCACTAAATAGAA TGCTAATCTCAGACTTAAATATTACAGNAAGGTGTACATGGGAAAACCNTCAGACAATTC TNCTTGGNTCAGGNCTCACTC >OriGene 3' read for NM_005414 unedited
GTACCGCGGCACGCAATCTAGTGTCGAGNTNTTTTTTTTTTTTTTTTTTTTTTTCAAAAA TTATCACATTTTATTTTTGAAAATTGCTGCTTAAAAAAAAATCTAACTATAACCACATTT AAACTGAAAAACAAATTACAACTGCTGCTTCTGTTTAGCAGCAGCACATAACAACACATT CTTCTTCCCCACTGGGCTCATGGGGTATGCTATAACCTCAGTAAAATTCCTCCTAGGGTA CACATTAAAAAGAAAAGAACTGGGGGAAATTCATTCCACAATCTGAACAAAGTAAAAGTA AATTTGGGGTATGTTAATGAGTACACCATATCAACTTAAAAAAATTGGGTTAATGAGAAA AGGGGAGATTCAACTCCTTGAGTAGCTCTATATACTTAAAATATAAAGCCACCATTGTAT CTAAAATTGAGTCTGAAATATGGATAACAAAATTGACCACAGCTATTTTCCATCACATAT TGCAGCTTTTAAAAAGTACACAATTGTTAAGTTTGCATTAAAAATATAAGCAGATTACCT ACTAGTTGAAACCATATAGTATTGTTACTAAGCTGATTTTTAAAAAAGTACAAAGAAACA TACATTTTCATTCATTTGGAAAATGTAAAAATTTAAGCACTAATATATAATCTCTTCACT TTCTCTGATCTACAAATGGATAGAATGCCTAGTTATCGTCATGCAGATAAATTCTTCAGA ATTCAATTACCAAAGCAAGCAACAAACAAAAAAAACCTTGTCAGTAATACACAGATGAAT CTCTTTAACAGTTTCTATTCTTTAGCAGTCTTTGATGATTTCAGAAATTGCAGCTTTAGC TCTTTAATATCATCTCTAACTTCTGGCTGGCTTCCCGTCCTGGCTGAGTTATCTGGGAGT TCTGGCTATCGGCCTCACATGGTCCATCTTGGCCAAGTCTGCCACTGCCTGCTATTAGCC CCTTGTCTTTTTTAATTGAGCCAGGNACATTTTGTTATTATCGCCCCATTTTTCCTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005414 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005414.2, NP_005405.1 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 2055 bp |
| Locus ID | 6498 |
| UniProt ID | P12757 |
| Domains | Ski_Sno |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) encodes the longest isoform (1, also known as snoN). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional effects of SKIL overexpression in prostate cancer cell lines
,Tuominen, J;,
Thesis
[SKIL]
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Transforming Growth Factor-ß/SMAD Target Gene SKIL Is Negatively Regulated by the Transcriptional Cofactor Complex SNON-SMAD4
,Angeles C. Tecalco-Cruz, Marcela Sosa-Garrocho, Genaro Vázquez-Victorio, Layla Ortiz-García, Elisa Domínguez-Hüttinger, and Marina Macías-Silva,
J. Biol. Chem., Aug 2012; 287: 26764 - 26776.
[SKIL]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209251 | SKIL (Myc-DDK-tagged)-Human SKI-like oncogene (SKIL), transcript variant 1 |
CNY 5,008.00 |
|
| RC209251L1 | Lenti ORF clone of Human SKI-like oncogene (SKIL), transcript variant 1, Myc-DDK-tagged |
CNY 7,408.00 |
|
| RC209251L2 | Lenti ORF clone of Human SKI-like oncogene (SKIL), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC209251L3 | Lenti ORF clone of Human SKI-like oncogene (SKIL), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209251L4 | Lenti ORF clone of Human SKI-like oncogene (SKIL), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG209251 | SKIL (tGFP-tagged) - Human SKI-like oncogene (SKIL), transcript variant 1 |
CNY 6,608.00 |
