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ADA (NM_000022) Human Untagged Clone
CAT＃: SC116886
ADA (untagged)-Human adenosine deaminase (ADA)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADA1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116886 sequence for NM_000022 edited (data generated by NextGen Sequencing)
ATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCACCTAGACGGA TCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGCCCTCCCAGCT AACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCACCCTTCCAGAC TTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGAGGCTATCAAA AGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGTATGTGGAGGTG CGGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCCTGGAACCAGGCT GAAGGGGACCTCACCCCAGACGAGGTGGTGGCCCTAGTGGGCCAGGGCCTGCAGGAGGGG GAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCGCCACCAGCCCAAC TGGTCCCCCAAGGTGGTGGAGCTGTGTAAGAAGTACCAGCAGCAGACCGTGGTGGCCATT GACCTGGCTGGAGATGAGACCATCCCAGGAAGCAGCCTCTTGCCTGGACATGTCCAGGCC TACCAGGAGGCTGTGAAGAGCGGCATTCACCGTACTGTCCACGCCGGGGAGGTGGGCTCG GCCGAAGTAGTAAAAGAGGCTGTGGACATACTCAAGACAGAGCGGCTGGGACACGGCTAC CACACCCTGGAAGACCAGGCCCTTTATAACAGGCTGCGGCAGGAAAACATGCACTTCGAG ATCTGCCCCTGGTCCAGCTACCTCACTGGTGCCTGGAAGCCGGACACGGAGCATGCAGTC ATTCGGCTCAAAAATGACCAGGCTAACTACTCGCTCAACACAGATGACCCGCTCATCTTC AAGTCCACCCTGGACACTGATTACCAGATGACCAAACGGGACATGGGCTTTACTGAAGAG GAGTTTAAAAGGCTGAACATCAATGCGGCCAAATCTAGTTTCCTCCCAGAAGATGAAAAG AGGGAGCTTCTCGACCTGCTCTATAAAGCCTATGGGATGCCACCTTCAGCCTCTGCAGGG CAGAACCTCTGA Clone variation with respect to NM_000022.2 534 a=>g >OriGene 5' read for NM_000022 unedited
GCAAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAAGCCGAG CGGCCACCGAGCCGGCAGAGACCCACCGAGCGGCGGCGGAGGGAGCAGCGCCGGGGCGCA CGAGGGCACCATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCA CCTAGACGGATCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGC CCTCCCAGCTAACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCAC CCTTCCAGACTTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGA GGCTATCAAAAGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGAT TTGNGAAGTGGGCCGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCC TGGAACCAGGCTGAAGGGGACCTCACCCCAGACAGGNTGGGTGGCCCTAGTGGGCCAGGG GCTGCAGGAGGNGGAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCG CCACCAGCCCAACTGGTCCCCAAGGNTGGGTGGAGCTGTGTAAAGAAGTACCAGCAGCAG ACCGGTGGTGGCCATTGACCTGGCTGGGAGATGAGACCATCCCCAGAAGCAGCCTCTTGC CTGGACATGTCCAGCCTACCAGNAGGCTTGTGAAGAAGCGCATTCACCGTACTGCCACCG GGGGAAGGGGGGGCTCGGGCCGAGTGTAAAAGAAGCTGGGGACATACTCAAGACGAGCGG TTGGGACACGCTACACACCCTGGAAGACAGGCCCTTATACAGCTGCGGCAGGAAACTGCC TTTCAGATTTGCCCTGGCCACTACTACTTGGGCCTGAAACCGACCCGGACAGGATATTTG GGTA >OriGene 3' read for NM_000022 unedited
GGGGGTGGGCCCCACNNCACCTTTTCCNNNNNGNNTTACTGTGNACCCGGCCGCTTTTNA NATTTTTTTTTTTTGCATGCCACCAGCCTGGGCTTCTTTATTGAGCACCAGNATTTCAGT ACAAGTTGCCACAGAAGGAGGGTTTCAGATTCAACCATGCCCATGTGCAAGGGCGCTGGT CCCTGGCCAGGGCACATAATCAGAGAAGTGACGCGGCCATGCCGAGGTATACGTGTGTGC AGAAATGGACACATAGGGTTCAGGAGCATCAGTAACTGACTATTGAGATCATGGTCTTCT TGGAAGGAATAAATGTAAAAATGTTGCTCAGCCCCACAGAGTTGGGGTGACTCCACAGGG TGAAGGCTTGGAGGAGTGGCGTCTTCAGAGGTTCTGCCCTGCAGAGGCTGAAGGTGGCAT CCCATAGGCTTTATAGAGCAGGTCGAGAAGCTCCCTCTTTTCATCTTCTGGGAGGAAACT AGATTTGGCCGCATTGATGTTCAGCCTTTTAAACTCCTCTTCAGTAAAGCCCATGTCCCG TTTGGTCATCTGGTAATCAGTGTCCAGGGTGGACTTGAAGATGAGCGGGTCATCTGTGTT GAGCGAGTAGTTAGCCTGGTCATTTTTGAGCCGAATGACTGCATGCTCCGTGTCCGGCTT CCANGCACCAGTGAGGTAGCTGGACCAGGGGCAGATCTCGAAGTGCATGTTTTCCTGCCG CAGCCTGTTATAAAAGGCCTGGTCTTCCAGGGTGTGGTAGCCGTGTCCCAGCCGCTCTGT CTTGAGTATGTCCACAGCCTTTTTTACTACTTTCGNCGAGCCCACCTCCCCGCCGTGGAC AGTACCGGGAATGCCGCTCTTACAGGCTCCTGGTAGGCCTGACATGTTCAGGCAAGAGCT GCTTCCTGGGAGGCCTCATTTCCAGCCAGTCATGGCCACACGGCTGCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000022 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000022.2, NP_000013.2 |
| RefSeq Size | 1566 bp |
| RefSeq ORF | 1092 bp |
| Locus ID | 100 |
| UniProt ID | P00813 |
| Domains | A_deaminase |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Primary immunodeficiency, Purine metabolism |
| Gene Summary | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia. [provided by RefSeq, Sep 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206679 | ADA (Myc-DDK-tagged)-Human adenosine deaminase (ADA) |
CNY 5,488.00 |
|
| RC206679L1 | Lenti-ORF clone of ADA (Myc-DDK-tagged)-Human adenosine deaminase (ADA) |
CNY 7,888.00 |
|
| RC206679L2 | Lenti-ORF clone of ADA (mGFP-tagged)-Human adenosine deaminase (ADA) |
CNY 5,890.00 |
|
| RC206679L3 | Lenti-ORF clone of ADA (Myc-DDK-tagged)-Human adenosine deaminase (ADA) |
CNY 7,888.00 |
|
| RC206679L4 | Lenti-ORF clone of ADA (mGFP-tagged)-Human adenosine deaminase (ADA) |
CNY 5,890.00 |
|
| RG206679 | ADA (tGFP-tagged) - Human adenosine deaminase (ADA) |
CNY 4,370.00 |
