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ATP6V0B (NM_004047) Human Untagged Clone
CAT＃: SC117630
ATP6V0B (untagged)-Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATP6F; HATPL; VMA16 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117630 sequence for NM_004047 edited (data generated by NextGen Sequencing)
ATGACGGGGCTAGCACTGCTCTACTCCGGGGTCTTCGTGGCCTTCTGGGCCTGCGCGCTG GCCGTGGGAGTCTGCTACACCATTTTTGATTTGGGCTTCCGCTTTGATGTGGCATGGTTC CTGACGGAGACTTCGCCCTTCATGTGGTCCAACCTGGGCATTGGCCTAGCTATCTCCCTG TCTGTGGTTGGGGCAGCCTGGGGCATCTATATTACCGGCTCCTCCATCATTGGTGGAGGA GTGAAGGCCCCCAGGATCAAGACCAAGAACCTGGTCAGCATCATCTTCTGTGAGGCTGTG GCCATCTACGGCATCATCATGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAGTGCC ACAGACCCCAAGGCCATCGGCCATCGGAACTACCATGCAGGCTACTCCATGTTTGGGGCT GGCCTCACCGTAGGCCTGTCTAACCTCTTCTGTGGAGTCTGCGTGGGCATCGTGGGCAGT GGGGCTGCCCTGGCCGATGCTCAGAACCCCAGCCTCTTTGTAAAGATTCTCATCGTGGAG ATCTTTGGCAGCGCCATTGGCCTCTTTGGGGTCATCGTCGCAATTCTTCAGACCTCCAGA GTGAAGATGGGTGACTAG Clone variation with respect to NM_004047.3 >OriGene 5' read for NM_004047 unedited
GTTCAAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGGACAGACTG CGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGCTCCGGCCCCGCCGTTCCGACCCCC GCCGCCGTCGCCGCCATGACGGGGCTAGCACTGCTCTACTCCGGGGTCTTCGTGGCCTTC TGGGCCTGCGCGCTGGCCGTGGGAGTCTGCTACACCATTTTTGATTTGGGCTTCCGCTTT GATGTGGCATGGTTCCTGACGGAGACTTCGCCCTTCATGTGGTCCAACCTGGGCATTGGC CTAGCTATCTCCCTGTCTGTGGTTGGGGCAGCCTGGGGCATCTATATTACCGGCTCCTCC ATCATTGGTGGAGGAGTGAAGGCCCCCAGGATCAAGACCAAGAACCTGGTCAGCATCATC TTCTGTGAGGCTGTGGCCATCTACGGCATCATCATGGCAATTGTCATTAGCAACATGGCT GAGCCTTTCAGTGCCACAGACCCCAAGGCCATCGGCCATCGGAACTACCATGCAGGCTAC TCCATGTTTGGGGCTGGCCTCACCGTAGGCCTGTCTAACCTCTTCTGTGGAGTCTGCGTG GGCATCGTGGGCAGTGGGGCTGCCCTGGCCGATGCTCAGAACCCCAGCCTCTTTGTAAAG ATTCTCATCGTGGAGATCTTTGGCAGCGCCATTGGCCTCTTTGGGGTCATCGTCGCAATT CTTCAGACCTCCAGAGTGAAGATGGGTGACTAGATGATATGTGTGGGTGGGGCCGTGCCT CACTNTTATTTATTGCTGGNTTTCCTGGGACAGCTGGAGCTGTGTCCCTTAACCTTTCAG AGGCTTGGTGTTCAGGGCCCTCCCCTGCACTCCCTCTTGCTGCGTGTTGATTTGGAGCAC TGCAGTCCAGGCCGAG >OriGene 3' read for NM_004047 unedited
AACTTTCCAGGGCCAGGNATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTG TTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTGACCCAAACAAATACCAAGTTTATTTCACAAACAC TAAGAAAATGGGTTGAGGGTGGAGGGGGGGGACACAAGTGCGCAACTGCACAAAATCAGC AGCAGCAGCCTGCTCCCCGCACTGAGGACTCGGCCTGGACTGGAGTGCCTCCAAATCAAC ACCCAACAAGAGGGGAGTGCCAGGAGGGCCCTGAACACCAAGCCTTTGAAAGGGTAAGGG ACACAGCTCCAGCTGTCCCAGGAAAACCAGCAATAAATAAAAGTGGGGGCCGGCCCCACC CACNNNATAATCTAATCACCCATTTTTACTTTGGAGGGCTGAAAAATTGCGACGATGACC CCAAAGAGGGCAATGGCGCTGCCAAAGATCTCCACGATGAGAATCTTTACAAAGAGCTGG GGTTCTGAGCATTGGGCAGGGCAGCCCCACTGGCCACGATGCCCACGCAAACTCCAAAAA GAGGGTAGACAGGCCTACGGTGAGGCCAGCCCCAAACTGGAGTAACCTGGTTGGTAATTC CCATGGCCGATGGCCTTTGGGGGTCTGTGGCACTGAAAGGCTCAACCCTGTTGCTAATGA CAATTGCCATGATGATGCGGTAGATGGGNNNATCCCACAGAAAATAATGCTGACCAGTTN TTGGTTTTGATCCCGGGGGGCCTTACTCCTNCCCATGAGGGGGGGAACCGGGATTTTAAA ATGCCCAGGCTGCCCCACCCCAGAAGGGAAATAGCTAGGCCATGCCCCACGTTGACGAAT AAAGGGGGAAGTCTCCGCAGGGACCTGCCCATCAAGCGGAGACCATAAAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004047 |
| Insert Size | 1070 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004047.2, NP_004038.1 |
| RefSeq Size | 1007 bp |
| RefSeq ORF | 618 bp |
| Locus ID | 533 |
| UniProt ID | Q99437 |
| Domains | ATP-synt_C |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| Gene Summary | This gene encodes a portion of the V0 domain of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Activity of this enzyme is necessary for such varied processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (1) encodes isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221823 | ATP6V0B (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 1 |
CNY 3,990.00 |
|
| RC221823L3 | Lenti-ORF clone of ATP6V0B (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 1 |
CNY 5,890.00 |
|
| RC221823L4 | Lenti-ORF clone of ATP6V0B (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 1 |
CNY 5,890.00 |
|
| RG221823 | ATP6V0B (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 1 |
CNY 4,370.00 |
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