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p35 (CDK5R1) (NM_003885) Human Untagged Clone
CAT＃: SC117693
CDK5R1 (untagged)-Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117693 sequence for NM_003885 edited (data generated by NextGen Sequencing)
ATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGC GCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAAC CTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCC AAGAAGAAGAACTCCAAGAAGGTGCAGCCCAACAGCAGCTACCAGAACAACATCACGCAC CTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAG CCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGG GGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAA CGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGC CGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGC GTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAAC GTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCAC GAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAG ATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGT TGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACAC TACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGG CTCCTCCTAGGCCTGGATCGGTGA Clone variation with respect to NM_003885.2 >OriGene 5' read for NM_003885 unedited
GGATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGGCGGCGGGGCC GCGGCGCGCATTGCGGAGGGCGCGGAGCGCAGGAGCTGCCGCCTGCCGGGCAGATCCAAG GGGGCAGCACGCTTCCCGGGAGCGCCCCCGCCTCCTCTCCGGGGCCGCCGCAGGCTCGGT GAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCGCAGGGGCGCGAGCCCCCGCCCGG CGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGC TGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACG CCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCG TGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCCAACAGCAGCTACCAGA ACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGT CCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGG GTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCG CAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGG GGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTG CTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGGCAGACCAGGGCTTT ATCACGCCGCCCAAACGTGGTCTTTCTCTCAGCCTGGGAGGGGGATGNTATCTCTCCCCC AGTGGGGCTCGGATCACAGCCTCNCAGNCCGTNCTGCTGACATGCCTGTACCTCTCTATN CACTGGNNNNCACGAGATCTCTACCGCTAG >OriGene 3' read for NM_003885 unedited
ACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTATGGATGGTCAGTGC ATTTTATTGAATCAGCACAGTACAAAAATAAATAAAAATAAGGGAAGGGGAATTAAATTA CAGCCAAACTGAGCTTCATGACTTTGTCAGATTATAAACCACACATACTCACAAACACAC TACACATACATACAAAATGAGACAAATATAAATTAATATTAACAATACCCACAGTTTGGT CAAAGAATAGCTACAGAAGAAATTGCACTAAAAAACCAACATACATCACACGTGTGTAAT TAGCAGTTTCAAATATACAGCTATGAATAATTCTGAGTGAAAAAAATGGCACATTTTCTT TTCATTGCAAGTTTAACTGTTGGAACAAAACTAAGTTCTAATAACTGCATGGAAAGAATA TATCAACCCTCCAGGTTTACAAGAAAAAGAGAAAAACCAAATCCTGAACTATTATTCCTG TGGCTTGTTCTGTGCATGGGTGTAATTTTCACAGCCATCTGTTCTAAGATTATTTTTCTT AAGACACAAAATGCTCCTGGTTTGCATGCACAATATCACCGTAAAAGCAACAATTAAAGA ATAAAGGCTCTTTTGTAAGTTTTTTTTTATTGTTGATCTAAAAGTAGTCTTCATAGCAGC ATGGCATATGGGTTCTTTGCTTGCACACTCAAGTATATCAGTTGTTCCTTTACACAATAC TGATGACCAGTGCAAGGGGAGATGACACCGACAGCTTTACACTGAGGGAACTAGAGAGTT CTTCCCTTATTCCCCACCACTTCCGATATGTCCACCTTCTCCGAAGATAAAAAAATAATA CGGAGAAAGAAAGGAATCAGACACGCACTGGGATCCTGTGGATGCAACGTTACTGCACAG TTCGCATCCAGGTTTCCGCAGCTGAAGTTGACCACACATGCGCCCNCTTCCGAGACTAGG ACTGAACCTCCTCTTNCTGCCACCAGGGCCTNCCACTCTGTCGCACGCGNAACAGATCTG ATCAAGCTGGGTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003885 |
| Insert Size | 4130 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003885.2, NP_003876.1 |
| RefSeq Size | 3870 bp |
| RefSeq ORF | 924 bp |
| Locus ID | 8851 |
| UniProt ID | Q15078 |
| Domains | CDK5_activator |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease |
| Gene Summary | The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phosphoregulation of the RNA-binding Protein Hu Antigen R (HuR) by Cdk5 Affects Centrosome Function
,Natalia Filippova, Xiuhua Yang, Peter King, and L. Burt Nabors,
J. Biol. Chem., Sep 2012; 287: 32277 - 32287.
[CDK5R1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206302 | CDK5R1 (Myc-DDK-tagged)-Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1) |
CNY 3,600.00 |
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| RC206302L1 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC206302L2 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mGFP tagged |
CNY 6,000.00 |
|
| RC206302L3 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206302L4 | Lenti ORF clone of Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mGFP tagged |
CNY 6,000.00 |
|
| RG206302 | CDK5R1 (tGFP-tagged) - Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1) |
CNY 5,200.00 |
