United States
Germany
Japan
United Kingdom
China
SUCLA2 (NM_003850) Human Untagged Clone
CAT＃: SC117714
SUCLA2 (untagged)-Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein
CNY 3,656.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A-BETA; A-SCS; LINC00444; MTDPS5; SCS-betaA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117714 sequence for NM_003850 edited (data generated by NextGen Sequencing)
ATGGCGGCCTCCATGTTCTACGGCAGGCTAGTGGCCGTGGCCACCCTTCGGAACCACCGG CCTCGGACGGCCCAGCGGGCTGCTGCTCAGGTTCTGGGAAGTTCTGGATTGTTTAATAAC CATGGACTCCAAGTACAGCAGCAACAGCAAAGGAATCTCTCACTACATGAATACATGAGT ATGGAATTATTGCAAGAAGCTGGTGTCTCCGTTCCCAAAGGATATGTGGCAAAGTCACCA GATGAAGCTTATGCAATTGCCAAAAAATTAGGTTCAAAAGATGTCGTGATAAAGGCACAG GTTTTAGCTGGTGGTAGAGGAAAAGGAACATTTGAAAGTGGCCTCAAAGGAGGAGTGAAG ATAGTTTTCTCTCCAGAAGAAGCAAAAGCTGTTTCTTCACAAATGATTGGGAAAAAATTG TTTACCAAGCAAACGGGAGAAAAGGGCAGAATATGCAATCAAGTATTGGTCTGTGAGCGA AAATATCCCAGGAGAGAATACTACTTTGCAATAACAATGGAAAGGTCATTTCAAGGTCCT GTATTAATAGGAAGTTCACATGGTGGTGTCAACATTGAAGATGTTGCTGCTGAGACTCCT GAAGCAATAATTAAAGAACCTATTGATATTGAAGAAGGCATCAAAAAGGAACAAGCTCTC CAGCTTGCACAGAAGATGGGATTTCCACCTAATATTGTGGAATCAGCAGCAGAAAACATG GTCAAGCTTTACAGCCTTTTTCTGAAATACGATGCAACCATGATAGAAATAAATCCAATG GTGGAAGATTCAGATGGAGCTGTATTGTGTATGGATGCAAAGATCAATTTTGACTCTAAT TCAGCCTATCGCCAAAAGAAAATCTTTGATCTACAGGACTGGACCCAGGAAGATGAAAGG GACAAAGATGCTGCTAAGGCAAATCTCAACTACATTGGCCTCGATGGAAATATAGGCTGC CTAGTAAATGGTGCTGGTTTGGCTATGGCCACAATGGATATAATAAAACTTCATGGAGGG ACTCCAGCCAACTTCCTTGATGTTGGTGGTGGTGCTACAGTCCATCAAGTAACAGAAGCA TTTAAGCTTATCACTTCAGATAAAAAGGTACTGGCTATTCTGGTCAACATTTTTGGAGGA ATCATGCGCTGTGATGTTATTGCACAGGGTATAGTCATGGCAGTAAAAGACTTGGAAATT AAAATACCTGTTGTGGTACGGTTACAAGGTACACGAGTCGATGATGCTAAGGCACTGATA GCGGACAGTGGACTTAAAATACTTGCTTGTGATGACTTGGATGAAGCTGCTAGAATGGTT GTAAAGCTCTCTGAAATAGTGACCTTAGCGAAGCAAGCACATGTGGATGTGAAATTTCAG TTGCCAATATGA Clone variation with respect to NM_003850.2 595 t=>a >OriGene 5' read for NM_003850 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGTCCGACTCAGAA ATGGCGGCCTCCATGTTCTACGGCAGGCTAGTGGCCGTGGCCACCCTTCGGAACCACCGG CCTCGGACGGCCCAGCGGGCTGCTGCTCAGGTTCTGGGAAGTTCTGGATTGTTTAATAAC CATGGACTCCAAGTACAGCAGCAACAGCAAAGGAATCTCTCACTACATGAATACATGAGT ATGGAATTATTGCAAGAAGCTGGTGTCTCCGTTCCCAAAGGATATGTGGCAAAGTCACCA GATGAAGCTTATGCAATTGCCAAAAAATTAGGTTCAAAAGATGTCGTGATAAAGGCACAG GTTTTAGCTGGTGGTAGAGGAAAAGGAACATTTGAAAGTGGCCTCAAAGGAGGAGTGAAG ATAGTTTTCTCTCCAGAAGAAGCAAAAGCTGTTTCTTCACAAATGATTGGGAAAAAATTG TTTACCAAGCAAACGGGAGAAAAGGGCAGAATATGCAATCAAGTATTGGTCTGTGAGCGA AAATATCCCAGGAGAGAATACTACTTTGCAATAACAATGGAAAGGTCATTTCAAGGTCCT GTATTAATAGGAAGTTCACATGNGTGGTGTCAACATTGAAGATGTTGCTGCTGAGACTCC TGAAGCATAATTAAAGAACCTATTGATATTGAAGAAGGCATCAAAAAGGAACAAGCTCTC CAGCTTGCACAGAAGATGGGATTTCCACCTAATATTGTGGAATCAGCAGCAGAAACATGG TCAAGCTTTACAGNCCTTTNNTCTGAAATACGATGCACCATGATAGAAATAAATCCATGG TGGNAAGATCAGATGGAGCTGTATTGTGTATGGGATGCAAGATCAATTTTGACTCTAATT CAGCCTATCGCCAAAA >OriGene 3' read for NM_003850 unedited
CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTATTGGGAAGATTTTTATTAACTTA AATTGACATTCTTAATTTTGTCTGTAAGTCCTTGGTATATATGCCTTTATTTGAAGCAAA CCTACAGGTGTTTCTTAATATGACAGAATCATGAAGACTTGCAGTTAATCAGTGTTTCCT AATGATTAAAACAATGTTCAAATAATTACAAAGTTACTTCATCAAAATACTTAGAAGAAT ATTCTGAGGAGTGTTTGAAAGCTCTGTTTATAAATAGTGATTGATACATTTATCATGTAT TTGGTGCTGAAGATAAACACTTTTTACATAAAACATTGTTTTAATATACTGCTCTACTAA TGAGGCTAGTTATTAGATATACTGTATTTTAACACTAAAGAATAAAGCTTTATCTTCGTA TTTATCTTATTTATAGGACTCTTATCAATGAAGAACTTTGTATCCAACAATAATAAACTG GCAAATTGCAAGTTACGTTTTTGTAGGAGAAGCAAAAAAGACTGGCTGCCACCAAAGAAA GAAATAACTGCCTATACATGCTTCGTCCAAACAGTCATTTCTGATGGACCATTAAATGCC ATCCAAATCCTTTTAAATGTTCGGGGCCTAAATGGCATTCCAATCCCCCCCCCTCAAAAG AAAACGAACACTCCCCCCAACACATTTTTTTTATGGATTTACCCATTTAACACCTTCACC CATCCACTGGGTTTTTAGATCTATTCGGAACTGTAATTCCCACTCCAATTTGCTTGTTTT CCTTAAGCACTTTTCACAACAGTGTACACCCTTCTACACTCTCTTCCCACCCTTCCAACC ATTTTGTAAGTACTCGGGCCCTTTAAGGCTTAACCTATAGACTCGGGTCCTGGTACCGCC CCAACAGTGTTTTATTTCACCTTTCCTGGCCTTAATTCCTGGGCACACCCCAACCGAGTT CTCTCAAGTGGACCACAACCCCCCTCTTTTCGAAGGAAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003850 |
| Insert Size | 2090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003850.1, NP_003841.1 |
| RefSeq Size | 2178 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 8803 |
| UniProt ID | Q9P2R7 |
| Domains | ATP-grasp, ligase-CoA |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism |
| Gene Summary | Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos
,Jesinkey, SR;Madiraju, AK;Alves, TC;Yarborough, OH;Cardone, RL;Zhao, X;Parsaei, Y;Nasiri, AR;Butrico, G;Liu, X;Molina, AJ;Rountree, AM;Neal, AS;Wolf, DM;Sterpka, J;Philbrick, WM;Sweet, IR;Shirihai, OH;Kibbey, RG;,
Cell Rep
,PubMed ID 31315053
[SUCLA2]
|
|
X-linked Sideroblastic Anemia Due to Carboxyl-terminal ALAS2 Mutations That Cause Loss of Binding to the ß-Subunit of Succinyl-CoA Synthetase (SUCLA2)
,David F. Bishop, Vassili Tchaikovskii, A. Victor Hoffbrand, Marie E. Fraser, and Steven Margolis,
J. Biol. Chem., Aug 2012; 287: 28943 - 28955.
[SUCLA2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205212 | SUCLA2 (Myc-DDK-tagged)-Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC205212L3 | Lenti ORF clone of Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205212L4 | Lenti ORF clone of Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,990.00 |
|
| RG205212 | SUCLA2 (tGFP-tagged) - Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
