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Beclin 1 (BECN1) (NM_003766) Human Untagged Clone
CAT＃: SC117750
BECN1 (untagged)-Human beclin 1, autophagy related (BECN1)
CNY 2,950.00
| Cited in 5 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATG6; beclin1; VPS30 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117750 sequence for NM_003766 edited (data generated by NextGen Sequencing)
ATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGC TGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATCCAG GAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAA GAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCTCGC AGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTGATT GGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACTGGG GACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAATGC ACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGTCAG AACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAGTTA CAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGAC GTGGAAAAGAACCGCAAGATAGTGGCAGAAAATCTCGAGAAGGTCCAGGCTGAGGCTGAG AGACTGGATCAGGAGGAAGCTCAGTATCAGAGAGAATACAGTGAATTTAAACGACAGCAG CTGGAGCTGGATGATGAGCTGAAGAGTGTTGAAAACCAGATGCGTTATGCCCAGACGCAG CTGGATAAGCTGAAGAAAACCAACGTCTTTAATGCAACCTTCCACATCTGGCACAGTGGA CAGTTTGGCACAATCAATAACTTCAGGCTGGGTCGCCTGCCCAGTGTTCCCGTGGAATGG AATGAGATTAATGCTGCTTGGGGCCAGACTGTGTTGCTGCTCCATGCTCTGGCCAATAAG ATGGGTCTGAAATTTCAGAGATACCGACTTGTTCCTTACGGAAACCATTCATATCTGGAG TCTCTGACAGACAAATCTAAGGAGCTGCCGTTATACTGTTCTGGGGGGTTGCGGTTTTTC TGGGACAACAAGTTTGACCATGCAATGGTGGCTTTCCTGGACTGTGTGCAGCAGTTCAAA GAAGAGGTTGAGAAAGGCGAGACACGTTTTTGTCTTCCCTACAGGATGGATGTGGAGAAA GGCAAGATTGAAGACACAGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAAC TCTGAGGAGCAGTGGACAAAAGCTCTCAAGTTCATGCTGACGAATCTTAAGTGGGGTCTT GCTTGGGTGTCCTCACAATTTTATAACAAATGA Clone variation with respect to NM_003766.3 >OriGene 5' read for NM_003766 unedited
AAACAAACTTTACTATAGGCGGCCGCGCAATTCGCACGAGGCGGGAAGTCGTTGAAGACA GAGCGATGGTAGTTCTGGAGGCCTCGCTCCGGGGCCGACCCGAGGCCACAGTGCCTCCGC GGTAGACCGGACTTGGGTGACGGGCTCCGGGCTCCCGAGGTGAAGAGCATCGGGGGCTGA GGGATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAGCTTCGTGTGCCAG CGCTGCAGCCAGCCCCTGAAACTGGACACGAGTTTCAAGATCCTGGACCGTGTCACCATC CAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGGAGAGACCCAGGAG GAAGAGACTAACTCAGGAGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCT CGCAGATTCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACTCTG ATTGGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAGACTGAAGGTCACT GGGGACCTTTTTGACATCATGTCGGGCCAGACAGATGTGGATCACCCACTCTGTGAGGAA TGCACAGATACTCTTTTAGACCAGCTGGACACTCAGCTCAACGTCACTGAAAATGAGTGT CAGAACTACAAACGCTGTTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAG TTACAGATGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTGGAA GACGTGGAAAAAGAACCGCAGATAGTGGCAGAAAATCTCGAGAAGGTCCANNGCTGAGCT GANAGACTGGATCANGNAGAAGCTCANTATCAGAGAGAATACAGTGAATTTAAACGACAG CAGCT >OriGene 3' read for NM_003766 unedited
CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAGTTTTCAG ACTGCAGCAAATCTTTTATTACAAATAATTAAATCTCTCCATAATGTCTCAAACAGTATC AAACACCATTTCATATCTCTAACACAGAGCAGAGTCGGCATTCAGTATAAGAACCAAGTG AAAAGTGTTAAATTTCAAGCATCTGATCACATCACATGGTGACCAGGTAAAGCTTAGATG TCATTTTCCCACATTATCCAACTGTGCATCTCAAACATATCCTCATCTCAGTAAAGACAA AAGTTTCTATTTCATATTGTTAAGTGCAGGAAGTTGAGAGAGATAAAAATCCAGTGAAAA CACATCAATCTCAATTCAACTCAGTTAAAAAAAAGAAAAGCAAATTTAAATTAGTTTTTT TCAGAGAAGAAAGGGAAAGGAGTCCATGGGGTTAAGAATCAAAACTGACCAGGGCTGGCA ACTATAGATGGCATGTTGTAGCTCTGGAAAGTATCTGTCACATGATATTTTAAAATAAAG TGGCTTTTGTGGATTTTTTCTTTTTTGGTATTGTAAACATGTACTGTTTAATATTACCCG AATTTAATTTAAAACATGTTTGCAAACAAAACAAAATTAAAAGCCTTTAAGGCAAACCCT CCCCCTAAGGAAAAAAGTCATTTGTTATAAAATTGTGAGGACACCNCAAGCAGACCCCAC TTAAGATTCGTCAGCATGAACTTGAGAGCTTTTGTCCACTGCTCCTCAGATTAAACTGGG TTTTGATGGAATAGACCCGCACTGCCTNCTGTGTCTCAATCTGGCTTTCTNCCATCCATC TGTAGGGAGACANAACGTGTCTCGCCTTCTCAACCTCTCTTTGACTGCTGCCCACAGTCA GAAAGCACATGCAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003766 |
| Insert Size | 2240 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003766.2, NP_003757.1 |
| RefSeq Size | 2144 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 8678 |
| UniProt ID | Q14457 |
| Domains | APG6 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of autophagy |
| Gene Summary | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 encode the same isoform. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Complex interplay between the length and composition of the huntingtin-derived peptides modulates the intracellular behavior of the N-terminal fragments of mutant huntingtin
,Milewski, M;Gawlinski, P;Bak, D;Matysiak, A;Bal, J;,
Eur. J. Cell Biol.
,PubMed ID 25773959
[BECN1]
|
|
Antagonism of tumoral prolactin receptor promotes autophagy-related cell death
,Wen, Y;Zand, B;Ozpolat, B;Szczepanski, MJ;Lu, C;Yuca, E;Carroll, AR;Alpay, N;Bartholomeusz, C;Tekedereli, I;Kang, Y;Rupaimoole, R;Pecot, CV;Dalton, HJ;Hernandez, A;Lokshin, A;Lutgendorf, SK;Liu, J;Hittelman, WN;Chen, WY;Lopez-Berestein, G;Szajnik, M;Ueno, NT;Coleman, RL;Sood, AK;,
Cell Rep 488-500 7 2. April 2014
,PubMed ID 24703838
[BECN1]
|
|
Inhibition of mTOR Kinase by AZD8055 Can Antagonize Chemotherapy-induced Cell Death through Autophagy Induction and Down-regulation of p62/Sequestosome 1
,Shengbing Huang, Zhineng J. Yang, Chunrong Yu, and Frank A. Sinicrope,
J. Biol. Chem., Nov 2011; 286: 40002 - 40012.
[BECN1]
|
|
Beclin 1 deficiency is associated with increased hypoxia-induced angiogenesis
,null,
Autophagy
,PubMed ID 21685724
[BECN1]
|
|
A natural BH3 mimetic induces autophagy in apoptosis-resistant prostate cancer via modulating Bcl-2–Beclin1 interaction at endoplasmic reticulum
,J Lian, X Wu, F He, D Karnak, W Tang, Y Meng, D Xiang, M Ji, T S Lawrence, L Xu,
Cell Death and Differentiation (25 June 2010) doi:10.1038/cdd.2010.74 Original Paper
[BECN1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201629 | BECN1 (Myc-DDK-tagged)-Human beclin 1, autophagy related (BECN1) |
CNY 5,488.00 |
|
| RC201629L1 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201629L2 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged |
CNY 7,888.00 |
|
| RC201629L3 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201629L4 | Lenti ORF clone of Human beclin 1, autophagy related (BECN1), mGFP tagged |
CNY 7,888.00 |
|
| RG201629 | BECN1 (tGFP-tagged) - Human beclin 1, autophagy related (BECN1) |
CNY 7,088.00 |
|
| SC324265 | BECN1 (untagged)-Human beclin 1, autophagy related (BECN1) |
CNY 5,488.00 |
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