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HCM (RNMT) (NM_003799) Human Untagged Clone
CAT＃: SC117770
RNMT (untagged)-Human RNA (guanine-7-) methyltransferase (RNMT)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cm1p; CMT1; CMT1c; hCMT1; hMet; MET; Met; RG7MT1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117770 sequence for NM_003799 edited (data generated by NextGen Sequencing)
ATGGCAAATTCTGCAAAAGCAGAAGAATATGAAAAGATGTCTCTTGAACAGGCAAAAGCG TCAGTGAATTCTGAAACAGAGTCTTCATTCAATATTAATGAAAACACAACAGCTTCTGGG ACTGGGCTTTCTGAAAAGACTTCTGTCTGTAGGCAAGTAGACATAGCAAGAAAGAGAAAA GAGTTTGAAGATGATCTTGTAAAGGAAAGTTCTAGTTGTGGGAAAGACACTCCATCCAAG AAGAGAAAACTTGATCCTGAAATTGTCCCAGAGGAAAAAGATTGTGGTGATGCTGAAGGC AATTCAAAGAAAAGAAAAAGAGAAACTGAGGATGTTCCAAAAGATAAATCTTCTACTGGA GATGGCACTCAAAATAAGAGAAAAATAGCACTTGAGGATGTTCCTGAAAAGCAGAAAAAT CTGGAAGAAGGACACAGCTCAACAGTGGCTGCCCATTACAATGAACTTCAGGAAGTTGGT TTGGAGAAGCGTAGTCAAAGTCGTATTTTTTACCTAAGAAACTTTAATAATTGGATGAAA AGTGTTCTCATTGGAGAATTTTTGGAAAAGGTACGACAGAAGAAAAAACGTGATATCACT GTTTTGGACCTGGGATGTGGTAAAGGTGGAGATTTGCTGAAATGGAAAAAAGGAAGAATT AACAAGCTAGTTTGTACTGATATTGCCGATGTTTCTGTCAAACAGTGTCAGCAGCGGTAT GAGGACATGAAAAATCGTCGTGATAGTGAATATATTTTCAGTGCAGAATTTATAACTGCT GACAGCTCAAAGGAACTTCTGATTGACAAATTTCGTGACCCACAAATGTGTTTTGACATC TGCAGTTGTCAGTTTGTCTGTCATTACTCATTTGAGTCTTATGAGCAGGCTGACATGATG CTGAGAAATGCGTGTGAGAGACTTAGCCCTGGGGGCTATTTTATTGGTACTACTCCCAAT AGCTTTGAATTGATAAGACGCCTTGAAGCTTCAGAAACAGAATCATTTGGAAATGAAATA TATACTGTGAAATTTCAGAAGAAAGGAGATTATCCTTTATTTGGCTGCAAATATGACTTC AACTTGGAAGGTGTTGTGGATGTTCCTGAATTCTTGGTCTATTTTCCATTGCTAAATGAA ATGGCAAAGAAGTACAATATGAAACTAGTCTACAAAAAAACATTTCTGGAATTCTACGAA GAAAAGATTAAGAACAATGAAAATAAAATGCTCTTAAAACGAATGCAGGCCTTGGAGCCA TATCCTGCAAATGAGAGTTCTAAACTTGTCTCTGAGAAGGTGGATGACTATGAACATGCA GCAAAGTACATGAAGAACAGTCAAGTAAGGTTACCTTTGGGAACCTTAAGTAAATCAGAA TGGGAAGCTACAAGTATTTACTTGGTGTTTGCCTTTGAGAAACAGCAGTGA Clone variation with respect to NM_003799.1 >OriGene 5' read for NM_003799 unedited
TACAAATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGTGGATGTG TGAACCTATTGGGTACTGTACAACTTCAAGCCTCGAAATCAGATAGGCACCACCAACCTC ATTTCCTGTTTCACCTTGATTTTCTGTGATACCAAAATCTCAGCTTCACAAAGTCATTGA AAGTGTTGGTTCATGAAGTTTTACCATCAATTCAAGTAATCATAAATGGCAAATTCTGCA AAAGCAGAAGAATATGAAAAGATGTCTCTTGAACAGGCAAAAGCGTCAGTGAATTCTGAA ACAGAGTCTTCATTCAATATTAATGAAAACACAACAGCTTCTGGGACTGGGCTTTCTGAA AAGACTTCTGTCTGTAGGCAAGTAGACATAGCAAGAAAGAGAAAAGAGTTTGAAGATGAT CTTGTAAAGGAAAGTTCTAGTTGTGGGAAAGACACTCCATCCAAGAAGAGAAAACTTGAT CCTGAAATTGTCCCAGAGGAAAAAGATTGTGGTGATGCTGAAGGCAATTCAAAGAAAAGA AAAAGAGAAACTGAGGATGTTCCAAAAGATAAATCTTCTACTGGAGATGGCACTCANAAT AAGAGAAAAATAGCACTTGANGATGTTCCTGANAAGCAGAAAAATCTGGAAGAAGGACAC AGCTCAACAGTGGGCTGCCATTTACATGAACTTCANGAAGTTGGTTTGNAGAAGCGTAGT CAAAGTCGTATTTTTTACCTAAGAAACTTTNATAATTGGATGAAAAAGTGTCTCATTGGA GAATTTTTGGAAAAAGTACGACAGAAGAAAAAACGTGATATCACTGTTTTTGACCTGGGA TGTTGTAAAAGTTGGAGAATTGCTGAAATGGAAAAAAGGNAGAATTACCAGCTATTTTGT ACTGATATGCCGAAGTTTCTGTCAAACATGTCCACACGGTATTAAGACATAN >OriGene 3' read for NM_003799 unedited
NTACTTTGGACGCGGCCGCTTCTANATTTTTTTTCAGAAAACAGTATGTGGTTTATTCAC ACAATGAAATATTATACAACCATGAGAATGAAAATCCATGTACAAATACATGCAACAAAA ATCTCACAAACATAATATTGGTGAAAAGAAACCAGATACGAAGAATGCATAATTCCATTT ATATATCTCCCCAAACCAGACAGAACTAATCTATGCGCTTAGAACTCAGGATAGTGGTTT CCCTTGGAGAGGTAGTTAACTGGAAGGGAATCTGACGGGGGGCTTCTGGGGTGCCGATTG TATGCACGTGCTCCACTTGCGAAAAATCATCGATGTGTTTCTATAGCTACACTTTAATAA AAACCTAAACAAACCGATTTGCCTCTGTCCGGATACTCGACTCAACACTGACAAAAATGT TTCCTTTGCTCTCAGCCTCTCACCTTTGGCTACACTGTTCCACCCGGCATGCTCAGTTCC TGCCACTCGCCAATCGCGAGGCTCTTCACCAGACCCCCCCCCCCCTGCCACGCCCGCTCT ATTATACTCTCCCCCTCCCCCCCCCCTCAACCACCATGTCCCCCCCTTCCATTTCCCGCC CTCTCTCCCCTCCCTCCCCCCCCCCCCCCCTCGCCTCCTCGCCTCCGCCCCCCTCATTGT CTACCCCCGGCCTTCGCGCGCCCCCGTCGCTCCTCCTCCATTTTTTGCCTTTTTCCCCGC CCGTTCCCCGCCCCGCCCCCCTTTCCCCCCGCGCCTCCTTTTCCTTCGCCCCCTCTTTCG CCCCCCCGGTCCGTCGTTCGGCCCGCGCCCCCCCGTTTCCCCCGCCCCCCGTTTAGCCCG CCGCCCGCGCCGCGGCACGCCCGCGCTTCCCGCGCCCGCCCGCCGCCCATTGCGCGCCGA GGCTCCGTCGCCCGCCCCCGCTCTTCCTCGTTGACCGCGCCGTTCCCTGCCCGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003799 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003799.1, NP_003790.1 |
| RefSeq Size | 6203 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 8731 |
| UniProt ID | O43148 |
| Domains | Pox_MCEL |
| Gene Summary | Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed:9790902, PubMed:9705270, PubMed:10347220, PubMed:11114884, PubMed:22099306, PubMed:27422871). Binds RNA containing 5'-terminal GpppC (PubMed:11114884).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 3' terminal exon, and differs in the 3' UTR and 3' coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207787 | RNMT (Myc-DDK-tagged)-Human RNA (guanine-7-) methyltransferase (RNMT) |
CNY 3,656.00 |
|
| RC207787L1 | Lenti ORF clone of Human RNA (guanine-7-) methyltransferase (RNMT), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207787L2 | Lenti ORF clone of Human RNA (guanine-7-) methyltransferase (RNMT), mGFP tagged |
CNY 5,890.00 |
|
| RC207787L3 | Lenti ORF clone of Human RNA (guanine-7-) methyltransferase (RNMT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207787L4 | Lenti ORF clone of Human RNA (guanine-7-) methyltransferase (RNMT), mGFP tagged |
CNY 5,890.00 |
|
| RG207787 | RNMT (tGFP-tagged) - Human RNA (guanine-7-) methyltransferase (RNMT) |
CNY 5,256.00 |
