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EIF3A (NM_003750) Human Untagged Clone
CAT＃: SC117805
EIF3A (untagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)
CNY 14,192.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF3; eIF3-p170; eIF3-theta; EIF3S10; P167; p180; p185; TIF32 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117805 sequence for NM_003750 edited (data generated by NextGen Sequencing)
ATGCCGGCCTATTTTCAGAGGCCGGAAAATGCCCTCAAACGCGCCAACGAATTTCTTGAG GTTGGCAAAAAGCAGCCTGCTCTGGATGTTCTTTATGATGTTATGAAAAGTAAAAAACAT AGAACATGGCAAAAGATACACGAACCAATTATGTTGAAATACTTGGAACTTTGCGTGGAT CTTCGCAAGAGCCACTTGGCAAAGGAGGGGTTATACCAGTATAAGAACATTTGTCAACAG GTGAACATAAAATCTCTGGAGGATGTTGTTAGGGCATATTTGAAAATGGCAGAGGAAAAA ACTGAAGCTGCTAAAGAAGAATCTCAGCAGATGGTCTTAGATATAGAGGATCTAGATAAT ATTCAAACTCCTGAGAGTGTTCTCCTAAGTGCTGTAAGTGGTGAAGACACTCAGGATCGT ACTGACAGATTACTTTTAACTCCATGGGTTAAATTCCTGTGGGAGTCTTACAGGCAGTGT TTGGACCTTCTTAGAAACAATTCTAGAGTAGAGCGCCTGTACCATGATATTGCCCAGCAA GCTTTCAAATTCTGCCTCCAATACACGCGTAAGGCTGAATTCCGTAAACTGTGTGACAAT TTGAGAATGCACTTATCGCAGATTCAGCGCCACCATAACCAAAGTACGGCAATCAATCTT AATAATCCAGAGAGCCAGTCCATGCATTTGGAAACCAGACTTGTTCAGCTGGACAGTGCT ATCAGCATGGAATTGTGGCAGGAAGCATTCAAAGCTGTGGAAGATATTCACGGGCTATTC TCCTTGTCTAAAAAACCACCTAAACCTCAGTTGATGGCAAATTACTATAACAAAGTCTCA ACTGTGTTTTGGAAATCTGGAAATGCTCTTTTTCATGCATCTACACTCCATCGTCTTTAC CATCTCTCTAGAGAAATGAGAAAGAATCTCACACAAGACGAGATGCAAAGAATGTCTACT AGAGTCCTTTTAGCCACTCTTTCCATCCCTATTACTCCTGAGCGTACGGATATTGCTCGA CTTCTGGATATGGATGGCATTATAGTTGAAAAACAGCGTCGCCTTGCAACACTACTAGGT CTTCAAGCCCCACCGACACGAATTGGCCTTATTAATGATATGGTCAGATTTAATGTACTA CAATATGTTGTCCCAGAAGTGAAAGACCTTTACAATTGGCTTGAAGTAGAATTTAACCCA TTAAAACTCTGTGAGCGAGTCACAAAGGTTCTAAATTGGGTTAGGGAACAACCTGAAAAG GAACCGGAATTGCAGCAGTATGTGCCACAACTGCAAAACAACACCATCCTCCGCCTTCTG CAGCAGGTGTCACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTTTGGTTCCT TTTGTTGATGCTTTCCAACTGGAACGGGCCATAGTAGATGCAGCCAGGCATTGCGACTTG CAGGTTCGTATTGATCACACTTCTCGGACCCTGAGTTTTGGATCTGATTTGAATTATGCT ACTCGAGAAGATGCTCCGATTGGTCCTCATTTGCAAAGCATGCCTTCAGAGCAGATAAGA AACCAGCTGACAGCCATGTCCTCAGTACTTGCAAAAGCACTTGAAGTCATTAAACCAGCT CATATACTGCAAGAGAAAGAAGAACAGCATCAGTTGGCTGTCACTGCATACCTTAAAAAT TCACGAAAAGAGCACCAGCGGATCCTGGCTCGCCGCCAGACAATTGAGGAGAGAAAAGAG CGCCTTGAGAGTCTGAATATTCAGCGTGAGAAAGAAGAATTGGAACAGAGGGAAGCTGAA CTCCAGAAAGTGCGGAAGGCTGAGGAAGAGAGGCTGCGCCAGGAAGCAAAGGAGAGAGAG AAGGAGCGTATCTTACAGGAACATGAACAAATCAAAAAGAAAACTGTCCGAGAGCGTTTG GAGCAGATCAAGAAAACAGAACTGGGTGCCAAAGCATTCAAAGATATTGATATTGAAGAC CTTGAGGAATTGGATCCAGATTTTATCATGGCTAAACAGGTTGAACAACTGGAGAAAGAA AAGAAAGAACTTCAAGAACGCCTAAAGAATCAAGAAAAGAAGATTGACTATTTTGAAAGA GCCAAACGTTTGGAAGAAATTCCTTTGATAAAGAGCGCTTACGAGGAACAGAGAATTAAA GACATGGATCTGTGGGAGCAACAAGAGGAAGAAAGAATTACTACAATGCAGCTAGAACGT GAAAAGGCTCTTGAACATAAGAATCGAATGTCACGAATGCTTGAAGACAGAGATTTATTC GTAATGCGACTCAAAGCTGCACGGCAGTCTGTTTATGAGGAAAAACTTAAACAGTTTGAA GAGCGATTAGCAGAAGAAAGGCATAATCGATTGGAAGAACGGAAAAGGCAGCGTAAAGAA GAACGCAGGATAACATACTATAGAGAAAAAGAAGAGGAGGAGCAGAGAAGGGCAGAAGAA CAAATGCTAAAAGAGCGGGAAGAGAGAGAGCGCGCCGAACGAGCAAAACGCGAGGAAGAG CTACGAGAGTATCAGGAGCGGGTGAAGAAATTAGAAGAAGTGGAAAGGAAAAAACGCCAA AGGGAGTTGGAAATTGAAGAACGAGAACGGCGTAGAGAGGAAGAGAGAAGACTTGGCGAT AGTTCCCTTTCTAGAAAGGACTCTCGTTGGGGAGATAGAGATTCAGAAGGCACCTGGAGA AAAGGACCTGAAGCAGATTCTGAGTGGAGAAGAGGCCCGCCAGAGAAGGAGTGGAGACGT GGAGAAGGGCGAGATGAGGACAGGTCTCATAGAAGAGATGAAGAGCGGCCCCGGCGTCTG GGGGATGATGAAGATAGAGAGCCCTCTCTTAGACCAGACGATGATCGGGTTCCCCGGCGT GGCATGGATGATGACAGAGGCCCTAGACGTGGTCCTGAGGAAGATAGGTTCTCTCGTCGT GGGGCAGACGATGACCGGCCTTCCTGGCGTAACACAGATGATGACAGGCCTCCCAGACGA ATTGCCGATGAAGACAGGGGAAACTGGCGTCATGCGGATGATGACAGACCACCTAGACGA GGACTGGATGAGGACAGAGGAAGCTGGCGAACAGCTGATGAGGACAGAGGACCAAGACGT GGGATGGATGATGACCGGGGGCCGAGGCGAGGAGGCGCTGATGATGAGCGATCATCCTGG CGTAATGCTGATGATGACCGGGGTCCCAGGCGAGGGTTGGATGATGATCGGGGTCCCAGG CGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGCATGGATGATGACCGGGGTCCCAGG CGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGGTTGGATGATGATCGAGGACCTTGG AGGAACGCCGATGATGACAGAATTCCCAGGCGTGGTGCAGAGGATGACAGGGGCCCTTGG AGAAACATGGATGATGATCGCCTTTCAAGACGTGCTGATGATGATCGGTTTCCCAGACGG GGTGATGACTCAAGACCTGGTCCTTGGAGACCATTAGTCAAGCCAGGTGGATGGAGAGAG AAAGAAAAAGCCAGAGAGGAGAGCTGGGGTCCACCTCGAGAATCAAGGCCATCAGAAGAA CGTGAATGGGACAGAGAAAAAGAAAGGGACAGAGATAATCAAGATCGGGAGGAGAATGAC AAGGACCCTGAGAGAGAAAGGGACAGAGAGAGAGATGTGGATCGAGAGGATCGCTTCAGA AGACCTAGGGATGAAGGTGGCTGGAGAAGAGGACCAGCTGAGGAATCTTCAAGCTGGAGA GACTCAAGTCGCCGGGACGATAGGGATAGGGATGACCGTCGCCGTGAGAGGGATGACCGG CGTGATCTAAGAGAAAGACGAGATCTAAGAGACGACAGGGACCGAAGAGGACCTCCACTC AGATCAGAACGTGAAGAAGTAAGTTCTTGGAGACGTGCTGATGACAGGAAAGATGACCGG GTGGAAGAGCGGGACCCTCCTCGTCGAGTTCCTCCCCCAGCTCTTTCAAGAGACCGAGAA AGAGACCGAGACCGAGAAAGAGAAGGTGAAAAAGAGAAGGCCTCATGGAGAGCTGAGAAA GATAGGGAATCTCTCCGTCGTACTAAAAATGAGACTGATGAAGATGGATGGACCACAGTA CGACGTTAA Clone variation with respect to NM_003750.2 939 t=>c >OriGene 5' read for NM_003750 unedited
GTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGGGCGACT GCTGGCGAGGCGCGTGGGACCTTACGCTGGTTCCCCTTCGTCTCCTCTCCCGGCCCGGGC CACTAGAGAGTTCGCTGACGCCGGGTGAGCTGAGCCTGCCGCCAAGATGCCGGCCTATTT TCAGAGGCCGGAAAATGCCCTCAAACGCGCCAACGAATTTCTTGAGGTTGGCAAAAAGCA GCCTGCTCTGGATGTTCTTTATGATGTTATGAAAAGTAAAAAACATAGAACATGGCAAAA GATACACGAACCAATTATGTTGAAATACTTGGAACTTTGCGTGGATCTTCGCAAGAGCCA CTTGGCAAAGGAGGGGTTATACCAGTATAAGAACATTTGTCAACAGGTGAACATAAAATC TCTGGAGGATGTTGTTAGGGCATATTTGAAAATGGCAGAGGAAAAAACTGAAGCTGCTAA AGAAGAATCTCAGCAGATGGTCTTAGATATAGAGGATCTAGATAATATTCAAACTCCTGA GAGTGTTCTCCTAAGTGCTGTAAGTGGTGAAGACACTCAGGATCGTACTGACAGATTACT TTTAACTCCATGGGTTAAATTCCTGTGGGAGTCTTACAGGCAGTGTTTGGACCTTCTTAG AAACAATTCTAGAGTAGAGCGCCTGTACCATGATATTGCCCAGCAAGCTTTCAAATTCTG CCTCCCATACACGCGTAAGGCTGAATTCCGTAAACTGTGTGACAATTTGAGAATGCACTT ATCGCAGATTCAGCGCCACCATAACCAAAGTACGGCAATCAATCTTTAATATTCCANAGA GCCCAGTCATGCATTTGGAAACCAGACTTGNTCAGCTGGACAGTGCTATCGCATGTAATT GTGGCAGGAGCATTCAAGCTGTGGAGGA >OriGene 3' read for NM_003750 unedited
TATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTCAACAAATGAT TGATGAAATAATGCAACACCCTTAAATTCCAAAAAATGGCATGGTTTTTCCAACCTCCTG TGGATATGGTGCATGATCAATCTATTATATAGGATTAATACAAGTTCATGCTTTTTGTGT TATGGGGAAACAACATTAAAGAATCCAATTTAGATTGGTTGAAGCACAAGCCATAATAAT CCTTGAATGTGATCAAACCTATTTAAGACACCCGCTTAAATCCATTATCTTGAGACTTAA CGCCGCACTGTGGTCCATCCATCTTTATCATTTTCACTTTTAGTACGACGCGAGAGATTC CCTATCTTTCTCAGCCCTCCATGAGGCCTTCTTTTTTTCACCTTCTCTTTCTCGGTCTCG GTCTCTTTCTCGGTTTTTTGAAAGACCTGGGGGCGGAACTCGACCAGGAGGGCTTTGCTC TTTCACCCGGCCATCTTCCCTGTTATATCATCTCCCACCAACTTACTTCCCTACGTTCCG ATCTGACCGGATGATCTCTCCGCCCCCTGTTGCCCCTTATATCTCTCCCTTTCCTTAGAT CCAGTCCGTTATTTCTTTTACGGCGACGGCCATCCCTACCCCCCATCCCCCCGTGACTCG TGCCCTTCCCTCTGTACCTTCCCTCTCGCTCTCCTATTCTCCACCTTCATCTTTGTCTCC CCATTCACCCTCCTCTTTACCCCTTTTTTTTCCTCCCTTTATCCCAGTGCCTTTTCTTCT TCCCCCACGCTCCCCATCTTTGTTCCTCCTTCTTCCCCGCTAATACATCCTCCCCCATGG CACTATTTCTTTTCCGCCCCAATTTATCTGCCCCCCCCCCTCTCTCTCCCCCCCCCTTCT TCCCCGTCCCTAGTCCCCCCCCCCCCCCGCCCTTCTCCCTTACCCCTCTCCCCCCCTCTC TTTCCTCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003750 |
| Insert Size | 4470 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003750.1, NP_003741.1 |
| RefSeq Size | 5256 bp |
| RefSeq ORF | 4149 bp |
| Locus ID | 8661 |
| UniProt ID | Q14152 |
| Domains | PCI |
| Gene Summary | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221766 | EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 14,184.00 |
|
| RC221766L1 | Lenti-ORF clone of EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 16,584.00 |
|
| RC221766L2 | Lenti-ORF clone of EIF3A (mGFP-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 15,680.00 |
|
| RC221766L3 | Lenti-ORF clone of EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 15,680.00 |
|
| RC221766L4 | Lenti-ORF clone of EIF3A (mGFP-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 16,584.00 |
|
| RG221766 | EIF3A (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit A (EIF3A) |
CNY 15,784.00 |
