EIF3A (NM_003750) Human Untagged Clone

CAT#: SC117805

EIF3A (untagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)



  "NM_003750" in other vectors (6)

CNY 14,192.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
EIF3A Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EIF3; eIF3-p170; eIF3-theta; EIF3S10; P167; p180; p185; TIF32
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117805 sequence for NM_003750 edited (data generated by NextGen Sequencing)
ATGCCGGCCTATTTTCAGAGGCCGGAAAATGCCCTCAAACGCGCCAACGAATTTCTTGAG
GTTGGCAAAAAGCAGCCTGCTCTGGATGTTCTTTATGATGTTATGAAAAGTAAAAAACAT
AGAACATGGCAAAAGATACACGAACCAATTATGTTGAAATACTTGGAACTTTGCGTGGAT
CTTCGCAAGAGCCACTTGGCAAAGGAGGGGTTATACCAGTATAAGAACATTTGTCAACAG
GTGAACATAAAATCTCTGGAGGATGTTGTTAGGGCATATTTGAAAATGGCAGAGGAAAAA
ACTGAAGCTGCTAAAGAAGAATCTCAGCAGATGGTCTTAGATATAGAGGATCTAGATAAT
ATTCAAACTCCTGAGAGTGTTCTCCTAAGTGCTGTAAGTGGTGAAGACACTCAGGATCGT
ACTGACAGATTACTTTTAACTCCATGGGTTAAATTCCTGTGGGAGTCTTACAGGCAGTGT
TTGGACCTTCTTAGAAACAATTCTAGAGTAGAGCGCCTGTACCATGATATTGCCCAGCAA
GCTTTCAAATTCTGCCTCCAATACACGCGTAAGGCTGAATTCCGTAAACTGTGTGACAAT
TTGAGAATGCACTTATCGCAGATTCAGCGCCACCATAACCAAAGTACGGCAATCAATCTT
AATAATCCAGAGAGCCAGTCCATGCATTTGGAAACCAGACTTGTTCAGCTGGACAGTGCT
ATCAGCATGGAATTGTGGCAGGAAGCATTCAAAGCTGTGGAAGATATTCACGGGCTATTC
TCCTTGTCTAAAAAACCACCTAAACCTCAGTTGATGGCAAATTACTATAACAAAGTCTCA
ACTGTGTTTTGGAAATCTGGAAATGCTCTTTTTCATGCATCTACACTCCATCGTCTTTAC
CATCTCTCTAGAGAAATGAGAAAGAATCTCACACAAGACGAGATGCAAAGAATGTCTACT
AGAGTCCTTTTAGCCACTCTTTCCATCCCTATTACTCCTGAGCGTACGGATATTGCTCGA
CTTCTGGATATGGATGGCATTATAGTTGAAAAACAGCGTCGCCTTGCAACACTACTAGGT
CTTCAAGCCCCACCGACACGAATTGGCCTTATTAATGATATGGTCAGATTTAATGTACTA
CAATATGTTGTCCCAGAAGTGAAAGACCTTTACAATTGGCTTGAAGTAGAATTTAACCCA
TTAAAACTCTGTGAGCGAGTCACAAAGGTTCTAAATTGGGTTAGGGAACAACCTGAAAAG
GAACCGGAATTGCAGCAGTATGTGCCACAACTGCAAAACAACACCATCCTCCGCCTTCTG
CAGCAGGTGTCACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTTTGGTTCCT
TTTGTTGATGCTTTCCAACTGGAACGGGCCATAGTAGATGCAGCCAGGCATTGCGACTTG
CAGGTTCGTATTGATCACACTTCTCGGACCCTGAGTTTTGGATCTGATTTGAATTATGCT
ACTCGAGAAGATGCTCCGATTGGTCCTCATTTGCAAAGCATGCCTTCAGAGCAGATAAGA
AACCAGCTGACAGCCATGTCCTCAGTACTTGCAAAAGCACTTGAAGTCATTAAACCAGCT
CATATACTGCAAGAGAAAGAAGAACAGCATCAGTTGGCTGTCACTGCATACCTTAAAAAT
TCACGAAAAGAGCACCAGCGGATCCTGGCTCGCCGCCAGACAATTGAGGAGAGAAAAGAG
CGCCTTGAGAGTCTGAATATTCAGCGTGAGAAAGAAGAATTGGAACAGAGGGAAGCTGAA
CTCCAGAAAGTGCGGAAGGCTGAGGAAGAGAGGCTGCGCCAGGAAGCAAAGGAGAGAGAG
AAGGAGCGTATCTTACAGGAACATGAACAAATCAAAAAGAAAACTGTCCGAGAGCGTTTG
GAGCAGATCAAGAAAACAGAACTGGGTGCCAAAGCATTCAAAGATATTGATATTGAAGAC
CTTGAGGAATTGGATCCAGATTTTATCATGGCTAAACAGGTTGAACAACTGGAGAAAGAA
AAGAAAGAACTTCAAGAACGCCTAAAGAATCAAGAAAAGAAGATTGACTATTTTGAAAGA
GCCAAACGTTTGGAAGAAATTCCTTTGATAAAGAGCGCTTACGAGGAACAGAGAATTAAA
GACATGGATCTGTGGGAGCAACAAGAGGAAGAAAGAATTACTACAATGCAGCTAGAACGT
GAAAAGGCTCTTGAACATAAGAATCGAATGTCACGAATGCTTGAAGACAGAGATTTATTC
GTAATGCGACTCAAAGCTGCACGGCAGTCTGTTTATGAGGAAAAACTTAAACAGTTTGAA
GAGCGATTAGCAGAAGAAAGGCATAATCGATTGGAAGAACGGAAAAGGCAGCGTAAAGAA
GAACGCAGGATAACATACTATAGAGAAAAAGAAGAGGAGGAGCAGAGAAGGGCAGAAGAA
CAAATGCTAAAAGAGCGGGAAGAGAGAGAGCGCGCCGAACGAGCAAAACGCGAGGAAGAG
CTACGAGAGTATCAGGAGCGGGTGAAGAAATTAGAAGAAGTGGAAAGGAAAAAACGCCAA
AGGGAGTTGGAAATTGAAGAACGAGAACGGCGTAGAGAGGAAGAGAGAAGACTTGGCGAT
AGTTCCCTTTCTAGAAAGGACTCTCGTTGGGGAGATAGAGATTCAGAAGGCACCTGGAGA
AAAGGACCTGAAGCAGATTCTGAGTGGAGAAGAGGCCCGCCAGAGAAGGAGTGGAGACGT
GGAGAAGGGCGAGATGAGGACAGGTCTCATAGAAGAGATGAAGAGCGGCCCCGGCGTCTG
GGGGATGATGAAGATAGAGAGCCCTCTCTTAGACCAGACGATGATCGGGTTCCCCGGCGT
GGCATGGATGATGACAGAGGCCCTAGACGTGGTCCTGAGGAAGATAGGTTCTCTCGTCGT
GGGGCAGACGATGACCGGCCTTCCTGGCGTAACACAGATGATGACAGGCCTCCCAGACGA
ATTGCCGATGAAGACAGGGGAAACTGGCGTCATGCGGATGATGACAGACCACCTAGACGA
GGACTGGATGAGGACAGAGGAAGCTGGCGAACAGCTGATGAGGACAGAGGACCAAGACGT
GGGATGGATGATGACCGGGGGCCGAGGCGAGGAGGCGCTGATGATGAGCGATCATCCTGG
CGTAATGCTGATGATGACCGGGGTCCCAGGCGAGGGTTGGATGATGATCGGGGTCCCAGG
CGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGCATGGATGATGACCGGGGTCCCAGG
CGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGGTTGGATGATGATCGAGGACCTTGG
AGGAACGCCGATGATGACAGAATTCCCAGGCGTGGTGCAGAGGATGACAGGGGCCCTTGG
AGAAACATGGATGATGATCGCCTTTCAAGACGTGCTGATGATGATCGGTTTCCCAGACGG
GGTGATGACTCAAGACCTGGTCCTTGGAGACCATTAGTCAAGCCAGGTGGATGGAGAGAG
AAAGAAAAAGCCAGAGAGGAGAGCTGGGGTCCACCTCGAGAATCAAGGCCATCAGAAGAA
CGTGAATGGGACAGAGAAAAAGAAAGGGACAGAGATAATCAAGATCGGGAGGAGAATGAC
AAGGACCCTGAGAGAGAAAGGGACAGAGAGAGAGATGTGGATCGAGAGGATCGCTTCAGA
AGACCTAGGGATGAAGGTGGCTGGAGAAGAGGACCAGCTGAGGAATCTTCAAGCTGGAGA
GACTCAAGTCGCCGGGACGATAGGGATAGGGATGACCGTCGCCGTGAGAGGGATGACCGG
CGTGATCTAAGAGAAAGACGAGATCTAAGAGACGACAGGGACCGAAGAGGACCTCCACTC
AGATCAGAACGTGAAGAAGTAAGTTCTTGGAGACGTGCTGATGACAGGAAAGATGACCGG
GTGGAAGAGCGGGACCCTCCTCGTCGAGTTCCTCCCCCAGCTCTTTCAAGAGACCGAGAA
AGAGACCGAGACCGAGAAAGAGAAGGTGAAAAAGAGAAGGCCTCATGGAGAGCTGAGAAA
GATAGGGAATCTCTCCGTCGTACTAAAAATGAGACTGATGAAGATGGATGGACCACAGTA
CGACGTTAA

Clone variation with respect to NM_003750.2
939 t=>c
>OriGene 5' read for NM_003750 unedited
GTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGGGCGACT
GCTGGCGAGGCGCGTGGGACCTTACGCTGGTTCCCCTTCGTCTCCTCTCCCGGCCCGGGC
CACTAGAGAGTTCGCTGACGCCGGGTGAGCTGAGCCTGCCGCCAAGATGCCGGCCTATTT
TCAGAGGCCGGAAAATGCCCTCAAACGCGCCAACGAATTTCTTGAGGTTGGCAAAAAGCA
GCCTGCTCTGGATGTTCTTTATGATGTTATGAAAAGTAAAAAACATAGAACATGGCAAAA
GATACACGAACCAATTATGTTGAAATACTTGGAACTTTGCGTGGATCTTCGCAAGAGCCA
CTTGGCAAAGGAGGGGTTATACCAGTATAAGAACATTTGTCAACAGGTGAACATAAAATC
TCTGGAGGATGTTGTTAGGGCATATTTGAAAATGGCAGAGGAAAAAACTGAAGCTGCTAA
AGAAGAATCTCAGCAGATGGTCTTAGATATAGAGGATCTAGATAATATTCAAACTCCTGA
GAGTGTTCTCCTAAGTGCTGTAAGTGGTGAAGACACTCAGGATCGTACTGACAGATTACT
TTTAACTCCATGGGTTAAATTCCTGTGGGAGTCTTACAGGCAGTGTTTGGACCTTCTTAG
AAACAATTCTAGAGTAGAGCGCCTGTACCATGATATTGCCCAGCAAGCTTTCAAATTCTG
CCTCCCATACACGCGTAAGGCTGAATTCCGTAAACTGTGTGACAATTTGAGAATGCACTT
ATCGCAGATTCAGCGCCACCATAACCAAAGTACGGCAATCAATCTTTAATATTCCANAGA
GCCCAGTCATGCATTTGGAAACCAGACTTGNTCAGCTGGACAGTGCTATCGCATGTAATT
GTGGCAGGAGCATTCAAGCTGTGGAGGA
>OriGene 3' read for NM_003750 unedited
TATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTCAACAAATGAT
TGATGAAATAATGCAACACCCTTAAATTCCAAAAAATGGCATGGTTTTTCCAACCTCCTG
TGGATATGGTGCATGATCAATCTATTATATAGGATTAATACAAGTTCATGCTTTTTGTGT
TATGGGGAAACAACATTAAAGAATCCAATTTAGATTGGTTGAAGCACAAGCCATAATAAT
CCTTGAATGTGATCAAACCTATTTAAGACACCCGCTTAAATCCATTATCTTGAGACTTAA
CGCCGCACTGTGGTCCATCCATCTTTATCATTTTCACTTTTAGTACGACGCGAGAGATTC
CCTATCTTTCTCAGCCCTCCATGAGGCCTTCTTTTTTTCACCTTCTCTTTCTCGGTCTCG
GTCTCTTTCTCGGTTTTTTGAAAGACCTGGGGGCGGAACTCGACCAGGAGGGCTTTGCTC
TTTCACCCGGCCATCTTCCCTGTTATATCATCTCCCACCAACTTACTTCCCTACGTTCCG
ATCTGACCGGATGATCTCTCCGCCCCCTGTTGCCCCTTATATCTCTCCCTTTCCTTAGAT
CCAGTCCGTTATTTCTTTTACGGCGACGGCCATCCCTACCCCCCATCCCCCCGTGACTCG
TGCCCTTCCCTCTGTACCTTCCCTCTCGCTCTCCTATTCTCCACCTTCATCTTTGTCTCC
CCATTCACCCTCCTCTTTACCCCTTTTTTTTCCTCCCTTTATCCCAGTGCCTTTTCTTCT
TCCCCCACGCTCCCCATCTTTGTTCCTCCTTCTTCCCCGCTAATACATCCTCCCCCATGG
CACTATTTCTTTTCCGCCCCAATTTATCTGCCCCCCCCCCTCTCTCTCCCCCCCCCTTCT
TCCCCGTCCCTAGTCCCCCCCCCCCCCCGCCCTTCTCCCTTACCCCTCTCCCCCCCTCTC
TTTCCTCCN
Restriction Sites NotI-NotI     
ACCN NM_003750
Insert Size 4470 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003750.1, NP_003741.1
RefSeq Size 5256 bp
RefSeq ORF 4149 bp
Locus ID 8661
UniProt ID Q14152
Domains PCI
Gene Summary RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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