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NSMAF (NM_003580) Human Untagged Clone
CAT＃: SC117865
NSMAF (untagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1
CNY 6,720.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FAN; GRAMD5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117865 sequence for NM_003580 edited (data generated by NextGen Sequencing)
ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGA TTTTCCTTGCTGCTGCTTAACTTGGAGGAGTACTACTTTGAACAGCATAGAGCCAATCAC ATTTTGCACAAGGGCAGTCACCATGAAAGGAAAATCAGAGGCTCCTTAAAAATATGTTCA AAATCGGTGATTTTTGAACCAGATTCAATATCCCAGCCCATCATCAAGATTCCTTTGAGA GACTGTATAAAAATAGGAAAGCATGGAGAAAATGGAGCCAATAGACACTTCACAAAGGCA AAATCTGGGGGTATTTCACTCATTTTCAGTCAGGTATATTTCATTAAAGAACATAATGTT GTTGCACCATATAAAATAGAAAGGGGCAAAATGGAATATGTTTTTGAATTGGATGTTCCC GGGAAAGTGGAAGATGTTGTGGAGACGTTGCTTCAGCTTCACAGAGCATCCTGCCTTGAC AAATTGGGTGACCAAACCGCCATGATAACAGCTATTTTGCAGTCTCGTTTAGCTAGAACA TCATTTGACAAAAACAGGTTCCAAAACATTTCTGAAAAGCTGCACATGGAATGCAAAGCA GAAATGGTGACGCCTCTGGTGACTAATCCTGGACACGTGTGCATCACGGACACAAACCTG TATTTTCAGCCCCTCAACGGCTACCCGAAACCTGTGGTCCAGATAACACTCCAAGATGTC CGCCGCATCTACAAAAGGAGGCACGGCCTCATGCCTCTGGGCTTGGAAGTATTTTGCACA GAAGATGATCTGTGTTCCGACATCTACCTAAAGTTCTATGAACCTCAAGATAGAGATGAT CTCTATTTTTACATTGCCACATACCTAGAGCACCATGTGGCGGAGCACACTGCTGAGAGC TACATGCTGCAGTGGCAGCGTGGACACCTTTCCAACTATCAGTACCTCCTTCACCTCAAC AACCTGGCCGACCGCAGCTGCAACGACCTCTCCCAGTACCCTGTGTTTCCATGGATAATA CATGATTATTCCAGCTCAGAACTAGATTTGTCAAATCCAGGAACCTTCCGGGATCTCAGT AAGCCAGTAGGGGCCCTAAATAAGGAACGGCTGGAGAGACTACTGACACGCTACCAGGAA ATGCCTGAACCAAAGTTCATGTATGGGAGTCACTACTCTTCCCCGGGTTATGTACTTTTT TATCTTGTTAGGATTGCACCAGAGTATATGCTGTGCCTGCAGAATGGAAGATTTGATAAT GCAGATAGAATGTTCAACAGTATTGCAGAAACTTGGAAAAACTGTCTGGATGGTGCAACG GATTTTAAAGAGTTAATTCCAGAATTCTATGGTGATGATGTGAGCTTTCTAGTCAATAGC CTGAAGTTGGATTTGGGAAAGAGACAAGGAGGACAGATGGTTGACGACGTGGAGCTTCCC CCTTGGGCTTCCAGTCCCGAGGACTTTCTCCAGAAGAGCAAAGATGCATTGGAAAGCAAT TATGTGTCTGAACACCTTCACGAGTGGATTGATCTAATATTTGGCTACAAACAAAAAGGG AGTGATGCAGTTGGGGCCCATAATGTATTTCATCCCCTGACCTATGAAGGAGGTGTAGAC TTGAACAGCATCCAGGATCCTGATGAGAAGGTAGCCATGCTTACGCAAATCTTGGAATTT GGGCAGACACCAAAACAACTATTTGTGACACCACATCCTCGAAGGATCACCCCAAAGTTT AAAAGTTTGTCCCAGACCTCCAGTTATAATGCTTCTATGGCAGATTCCCCAGGTGAAGAG TCTTTTGAAGACCTGACCGAAGAAAGCAAAACACTGGCCTGGAATAACATCACCAAACTG CAGTTACACGAGCACTATAAAATCCACAAAGAAGCAGTTACTGGAATCACGGTCTCTCGC AATGGATCTTCAGTATTCACAACATCCCAAGATTCCACCTTGAAGATGTTTTCTAAAGAA TCAAAAATGCTACAAAGAAGTATATCATTTTCAAATATGGCTTTATCGTCTTGTTTACTT TTACCAGGAGATGCCACTGTCATAACTTCTTCATGGGATAATAATGTCTATTTTTATTCC ATAGCATTTGGAAGACGCCAGGACACGTTAATGGGACATGATGATGCTGTTAGTAAGATC TGTTGGCATGACAACAGGCTATATTCTGCATCGTGGGACTCTACAGTGAAGGTGTGGTCT GGTGTTCCTGCAGAGATGCCAGGCACCAAAAGACACCACTTTGACTTGCTGGCCGAGCTG GAACATGATGTCAGTGTAGATACAATCAGTTTAAATGCTGCAAGCACACTGTTAGTTTCC GGCACCAAAGAAGGCACAGTGAATATTTGGGACCTCACAACGGCCACCTTAATGCACCAG ATTCCATGCCATTCAGGGATTGTATGTGACACTGCTTTTAGCCCAGATAGTCGCCATGTC CTCAGCACAGGAACAGATGGCTGTCTTAATGTCATTGATGTGCAGACAGGAATGCTCATC TCCTCCATGACATCAGATGAGCCCCAGAGGTGCTTTGTCTGGGATGGAAATTCCGTTTTA TCTGGCAGTCAGTCTGGTGAACTGCTCGTTTGGGACCTCCTTGGAGCAAAAATCAGTGAG AGAATACAGGGCCACACAGGTGCTGTGACATGTATATGGATGAATGAACAGTGTAGCAGT ATCATCACAGGAGGGGAAGACAGACAAATTATATTCTGGAAATTGCAGTATTAA Clone variation with respect to NM_003580.3 >OriGene 5' read for NM_003580 unedited
NTTGTTCGAAATTTGGTATACGACTCACTATAGGGCGGGCCGCGAATCGGCACCAGCCGG AGTCCCCACGCGAGGATGCTGCGGTGAGCGCGGCCGGGACGCCGCGTCGGGCTCTCGGCC GCCCAGCGGCGCCGCAGGGGAAGCCAGGCCGGCAGCCGCGCGCTCCCCAGCCGGCCACCA ATCCCGCCCTCCCCGGCTCCTCCGGACCTCCTCGGCAGGCGGCGGCGGGGCCTGCCTGTG CGCTCTGCGCCCGCCCGCGCCTACCCTCCATGGCGTTTATCCGGAAGAAGCAGCAGGAGC AGCAGCTGCAGCTCTACTCCAAGGAGAGATTTTCCTTGCTGCTGCTTAACTTGGAGGAGT ACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAAAGGA AAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCAATAT CCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGAGAAA ATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTCAGTC AGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGCAAAA TGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACGTTGC TTCAGCTTCACAGAGCATCCTGCCTTGGACAATGGGTGACCAAACCGNCATGATAACAGC TANTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGTTCCAAAACATTTCT GAAAAGCTGCACATGGAATGCANAGCAGNAATGGGTGACGCCTCTGGTGACTAATCCTGG ACACGTGTGCATCACGGACACAAACCTGTATTTTCAGNCCCTCAC >OriGene 3' read for NM_003580 unedited
TACGGATTCCCGGCTCCAGTGTTTTTAAGATACCAAACAGAATAGCTGTAATGAAATACA AACCGTTCCTATACACAGTAAACTTACTAAGAACTGCTAATCCTTCTCCAGGTTCTTAGA ATCTGGGTACAGCATTATAGAAAGTAACCACGCTATGAAAACACACACCCAAAACCCGAT GGGTGGCAATTGTGATAGATTAGGTCGCCTCCCTCATATTCAGAAGTAGCAGAGAATACT GCACATTTTCTTGGAAAATTTCTTAATTGTTCAGTGCTTTCTGACAAGTTTCCCCAGCCC AATTTATCTCTTAGCTATTCTCTGCTACTTAGTCCTGTCTTCTGACAATCATCATACGGG GACGATCATCTGCCTTACAGTGTAACATGTTTTTCCTAACACAGCCGCATTTTATCTGCC CTAAGAGAATAGCAACTAATGGCTTTCAATGAAGTCACCATGATTTAGAAAGTTTAAAAC CACAAATTTTCCATGTGGCAAAACTTCTATTACTAACATTGCACATTCACCAGTCCGTTT AAAAGTTCGGCTTAAAAATGCGTTAAATAGAGGNCAATTTAATATTCAGGAGAGGAAAAG GCCCTTAATACTGCAATTCCCAGAATTTAATCCGCCTGGCTTGCCCTCCTGCGATGATCC TGCTACCCTGTTGATTCCTTCCTATTCCTGTTACATGCCCTGGGGGGCCCTGTGTTGCCT CGCTGGTTGTTGCTGGTGGGGGGGGCCTTCGGGGCGGGCGCCCAGACTGCCTGCGGTAGA AACGGGTTTTTCTCGCCGACGAGGCCCCTCGGGGCTCCCCCGTGGTTTGGGGGCATAACC TACCTGTGGCGCGTGGNCGCCTGTGGCCGGGAGTGGGCCTTGGCGTGCCTGGGGCTTTTG GGGGCGGTCCGGCGGTGCCCCGCCCGGGGGGCGTCCCGATCCCCGTTGTCCCACGTCTAA TTATTATAAAAAAAAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003580 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003580.2, NP_003571.2 |
| RefSeq Size | 3564 bp |
| RefSeq ORF | 2754 bp |
| Locus ID | 8439 |
| UniProt ID | Q92636 |
| Domains | Beach, WD40, GRAM |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207925 | NSMAF (Myc-DDK-tagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1 |
CNY 6,720.00 |
|
| RC207925L3 | Lenti ORF clone of Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC207925L4 | Lenti ORF clone of Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RG207925 | NSMAF (tGFP-tagged) - Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1 |
CNY 8,320.00 |
