Cullin 3 (CUL3) (NM_003590) Human Untagged Clone

CAT#: SC117870

CUL3 (untagged)-Human cullin 3 (CUL3)



  "NM_003590" in other vectors (7)

CNY 8,448.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-CUL3 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CUL-3; NEDAUS; PHA2E
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117870 sequence for NM_003590 edited (data generated by NextGen Sequencing)
ATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCC
TTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCA
ATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAAT
GCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTT
GTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAAC
TTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGA
GACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTAC
AATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCAT
CTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGA
GGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTC
TATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAA
AGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGA
ATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATT
GTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAG
AATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTAC
AAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTAT
TTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGAC
TATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTC
AACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTC
AACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTC
AAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGG
TTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTT
CTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATTTAAGTTAAAGACT
GAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCA
AACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGT
GTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCA
AAGTGCAACATCCCACCAGCTCCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTA
GCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAAT
GCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCA
CAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACC
ATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACA
GATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAACCAACA
CAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACA
GTTAATGATCAGTTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAA
CAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAA
CATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAAT
GTTCTAGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTT
ATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGAT
CGCAAAGTATACACATATGTAGCATAA

Clone variation with respect to NM_003590.3
1367 c=>t;1581 a=>t;1992 a=>g
>OriGene 5' read for NM_003590 unedited
GCACGAGGCGGAGGAGGAGAATGAGGAGGAGGAGGACGACGTTCGGCCTGCGCAGTGAGA
TGTTTGTCCGTCGCCGCCGCCGCCGCCATCGCGGAGGAGCGCGATAAAGGGAGCCGAGCC
GGACGTGAGGGGGACCCCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAG
TCGCCAGAGTCCGAGCCGTCGTCGCCGCCGCCCCCGCCGCCGTCGCGGCTGCCCATGGGC
CGGCCAGCTCCCCAGCCCTGCCCGGGGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTA
GCCTGCCTGCCCGCCTTAAATGTGACACCGGCGTTGCGGAGCGCGACCTGTAGCCGCCGC
CGGGGAGGGGACTAGCACCATGTCGAATCTGAGCATAGGCACGGGCATCCGGAAGGACAC
CAAGATGCAGATCCGGGCCTTTTCGATGACCATGGATGAAAAATATGTAAACAGCATTTG
GGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAATAATAACAGTGGTCTTAGTTC
TGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTA
CACTGGA
>OriGene 3' read for NM_003590 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAATCCACAATTTTAT
TGTAATGAGCTGTATTTTCTAAATTTCTCTTTTATTTTCCTGTTTTCATACAGTAAAGAA
AACAAAACGCAGCACATTCACATTTTCCCGAGGAACTGTAAAGATGATCTCTACAGGGAT
AAACGTTGTAAAGCCTGAAGCTCAATCAACTGATGTTGGAAACTCTCAAAGGGAGTAAAG
GCTTGATCTCAATGGTCTAGAACATGTACTGTAATTTAATAGAAGAGATGGTCGTCTTAA
TATTTAATGATTTAAAAGAACTTCCCAGTCCATGCGAAGAGTACAGTCCAAGAATAAATC
AAATTTCTGAACGCATTTTATGCTACATATGTGTATACTTTGCGATCCTCAGGTGTTCGT
GCCAAATATTCTCTCTCAATAAGTCCTTCAATACGTTTCTTAATAACAACTGGACTTGGT
AAGAATCGCGCCTTCAACTGCTGAGTTACCTCCGCTACTAGAACATTGTGCTGCATCTTC
TTTCTAGATTTCATTATCCGCACTATAGCAGCTTCTATCTCATGTTTTCTGTCGGCGTCT
ACTTTCTGCCTTGTTTCTTTCCTCTCTGGGTCGGATTCACCTTGTTTGGCAGCAACTGTT
TGAATCTTGACTCTGTGTAGTTTGGATGTGAACTGATCATTACCTGTAAATATATGACCA
TTTTCCTATTTCCTTTGATTTGGGGTCTTTTGTAAGAACCCGCTGTGTTGTTTTACCACC
AGCGAGGGACTGTAGGNCTCTAACAGCTCCTCTTTCAGGATATCTGGCCCTTGCTGAATT
TCCTCAAAGTGGAATTTNCTCTTTTATAAAAGAGCATAAATGGTCATCTGGAAAGGGGAA
CTTGA
Restriction Sites NotI-NotI     
ACCN NM_003590
Insert Size 5580 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003590.2, NP_003581.1
RefSeq Size 6787 bp
RefSeq ORF 2307 bp
Locus ID 8452
UniProt ID Q13618
Domains CULLIN
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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