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ZNF207 (NM_003457) Human Untagged Clone
CAT＃: SC117945
ZNF207 (untagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1
CNY 5,488.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BuGZ; hBuGZ |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117945 sequence for NM_003457 edited (data generated by NextGen Sequencing)
ATGGGTCGCAAGAAGAAGAAGCAGCTGAAGCCGTGGTGCTGGTATTGTAATAGAGATTTT GATGATGAGAAGATCCTTATTCAGCACCAAAAAGCAAAGCATTTTAAATGCCATATATGT CACAAGAAATTGTATACAGGACCTGGCTTAGCTATTCATTGCATGCAGGTACATAAAGAA ACAATAGATGCCGTACCAAATGCAATACCTGGAAGAACAGACATAGAGTTGGAAATATAT GGTATGGAAGGTATTCCAGAAAAAGACATGGATGAAAGACGACGACTTCTTGAACAGAAA ACACAAGAAAGTCAAAAAAAGAAGCAACAAGATGATTCTGATGAATATGATGATGACGAC TCTGCAGCCTCAACTTCATTTCAGCCACAGCCTGTTCAACCTCAGCAAGGTTATATTCCT CCAATGGCACAGCCAGGACTGCCACCAGTACCAGGAGCACCAGGAATGCCTCCAGGCATA CCTCCATTAATGCCAGGTGTTCCTCCTCTGATGCCAGGAATGCCACCAGTTATGCCAGGC ATGCCACCTGGAATGATGCCAATGGGTGGAATGATGCCACCTGGACCAGGAATACCACCT CTGATGCCTGGAATGCCACCAGGTATGCCCCCACCTGTTCCACGTCCTGGAATTCCTCCA ATGACTCAAGCACAGGCTGTTTCAGCGCCAGGTATTCTTAATAGACCACCTGCACCAACA GCAACTGTACCTGCCCCACAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAG ATGGGGACACCTGTCACAAGCTCAAGTACAGCTTCATCCAATTCAGAAAGTCTGTCTGCA TCTTCTAAAGCTCTGTTTCCTAGCACAGCACAAGCTCAGGCAGCTGTCCAAGGACCTGTT GGTACAGATTTCAAACCCTTAAATAGTACCCCTGCAACAACTACAGAACCCCCAAAGCCT ACATTCCCTGCTTATACACAGTCTACAGCTTCAACAACTAGTACAACAAATAGTACTGCA GCTAAACCAGCGGCTTCAATAACAAGTAAGCCTGCTACACTTACAACAACTAGTGCAACC AGTAAGTTGATCCATCCAGATGAGGATATATCCCTGGAAGAGAGAAGGGCACAGTTACCT AAGTATCAACGTAATCTTCCTCGGCCAGGACAGGCCCCCATCGGTAATCCACCAGTTGGA CCAATTGGAGGTATGATGCCACCACAGCCAGGCATCCCACAGCAACAAGGAATGAGACCC CCAATGCCACCTCATGGTCAGTATGGTGGTCATCATCAAGGCATGCCAGGATACCTTCCT GGTGCTATGCCCCCGTATGGGCAGGGACCGCCAATGGTGCCCCCTTACCAGGGTGGGCCT CCTCGACCTCCGATGGGAATGAGACCTCCTGTAATGTCGCAAGGTGGCCGTTACTGA Clone variation with respect to NM_003457.3 >OriGene 5' read for NM_003457 unedited
NGGGGGGTTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGG GGAACGAGGCCGTCGGCCATTTTGTGTCTGCTTCCTGTGGGACGTGGTGGTAGCCGTTGG GTTGGGAAAGTGAGGGATTTTTGGCCTCGTTTCTCCTGCTTCTTTTCTCCTCCCTTTTAC TTTGCCGGTAGAACACAGTTATGGGTCGCAAGAAGAAGAAGCAGCTGAAGCCGTGGTGCT GGTATTGTAATAGAGATTTTGATGATGAGAAGATCCTTATTCAGCACCAAAAAGCAAAGC ATTTTAAATGCCATATATGTCACAAGAAATTGTATACAGGACCTGGCTTAGCTATTCATT GCATGCAGGTACATAAAGAAACAATAGATGCCGTACCAAATGCAATACCTGGAAGAACAG ACATAGAGTTGGAAATATATGGTATGGAAGGTATTCCAGAAAAAGACATGGATGAAAGAC GACGACTTCTTGAACAGAAAACACAAGAAAGTCAAAAAAAGAAGCAACAAGATGATTCTG ATGAATATGATGATGACGACTCTGCAGCCTCAACTTCATTTCAGCCACAGCCTGTTCAAC CTCAGCAAGGTTATATTCCTCCAATGGCACAGCCAGGACTGCCACCAGTACCAGGAGCAC CAGGAATGCCTCCAGGCATACCTCCATTAATGCCAGGTGTTCCTCCTCTGATGCCAGGAA TGCCACCAGTTATGCCAGGCATGCCACCTGGATTGCATCATCAGAGAAAATACACCCAGT CATTNTTGCGGTGAAAACATAATGATGCCAATGGGNTGGAATGATGCCACCTGGACCAGG AATACCACCTCTGATGCCCTGGATGCCACCAGGTATGCCCCCACCTGNTCCACGTNCTGG AATTNCTNCATGACTCAGCCC >OriGene 3' read for NM_003457 unedited
NGGGTTTACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAA ATTTTTCTCTCATTTTATTACAATGAAGTTTAACAGTACAGAAAAGTCACATGACCTTGG TGGGTCAGATTTCTTAAACCCTGCAACATGAGGAACTCTAAATACATTAAATATATGTTA CACATTCAGACTTCCAATGTACAAGTACTTGGAAACAGTTACAGCCCTCACCAAGCTAGG TTGGGGACATGAAGTCCAACAGCATCTGAAATGCTGTGAAGGCAAACTTTACAAATAGCA ATGTAAGCTTACAGAACTTGCCTTTACTAGGGTGGTATGTTCATGTAATTCCAGCGCCTT CACAATTTAACGAACCAAATTTACTATACTTCACTTCTAAAAACCTAGATGAAACATGAA AAAGAAGCCATACAGTATAAATTGCAACTTCAGGAAAAGTTCCTGCTTTATTATTCCAAA TAATTTTTCCCATGTAAAATAAATGTCCAATAGTTCCTGGTATGTGGGAAGATAATGTTT GTTAAAGTCACAATGAAGCCTTATTGACGGAAGCTTGGCTATATAAACAGCACAAGTTGA GAAAAGGTTTAATCTCCAAACCTATTAGACTGGATGAAGTAAGATCAGTAACGGCCACCT TGCGACATTACAGGAGGTCTCATTCCCATCCGGAGGTCGAGGAGGCCCACCCTGGTAAGA TGGCACCATTGGGCGGTCCCTGCCCCATACGGGGCATAGCACCCAGAAAGGATCCTGGCA TGCCTTGATGATGACCACCTACTGACCATGAGGTGGCATTGGGGGTCTCATTCCTTGTTG CTGGGGGAGCCCTGGCGTGGGGGCATCATAACTCCAATTGTGCCATGGTGGGATAACCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003457 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003457.1, NP_003448.1 |
| RefSeq Size | 2347 bp |
| RefSeq ORF | 1437 bp |
| Locus ID | 7756 |
| UniProt ID | O43670 |
| Domains | zf-C2H2 |
| Protein Families | Transcription Factors |
| Gene Summary | Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) lacks an in-frame exon in the coding region, compared to variant 3. It encodes isoform a, which is shorter than isoform c. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223933 | ZNF207 (Myc-DDK-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 5,856.00 |
|
| RC223933L1 | Lenti-ORF clone of ZNF207 (Myc-DDK-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 8,256.00 |
|
| RC223933L2 | Lenti-ORF clone of ZNF207 (mGFP-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 6,180.00 |
|
| RC223933L3 | Lenti-ORF clone of ZNF207 (Myc-DDK-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 6,180.00 |
|
| RC223933L4 | Lenti-ORF clone of ZNF207 (mGFP-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 6,180.00 |
|
| RG223933 | ZNF207 (tGFP-tagged) - Human zinc finger protein 207 (ZNF207), transcript variant 1 |
CNY 4,750.00 |
