USP5 (NM_003481) Human Untagged Clone

CAT#: SC117962

USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2



  "NM_003481" in other vectors (6)

CNY 6,120.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-USP5 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ISOT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_003481 edited
GGGCGGCCGCGAATTCGGCACGAGGGAGAAGCTGCTGCCGGTGTCATGGCGGAGCTGAGT
GAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACCGGGTC
CACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATC
TGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACC
GGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCT
ACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGC
GGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGAGGATGTGAAGATTGTCATTTTG
CCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCTGACATTGTCAGAGAT
CGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGCAGGAG
GTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAG
TTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGA
GAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGAT
GGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCT
GTCAAGCTGGGCACCATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGAC
ATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAG
ATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGATTGGT
GAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTAC
ACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC
AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCC
CCGACGGACCCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTC
TCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAG
AAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTCATCGGCAAGGGCCAC
CCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTATCAAC
ATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTG
GAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTAC
ATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTAC
GAGGAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCC
CAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTC
TGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTC
CCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGCCCAAG
AAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACA
GGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGAT
GAGCCCAAAGCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTC
CCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCC
ATGAACTGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCT
GGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTG
ACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACG
AACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCT
GAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTG
CCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGT
CACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA
TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGC
TACATCTACTTCTACCAGAGAGTGGCCAGCTAAGAGCCTGCCTCACCCCTTACCAATGAG
GGCAGGGGAAGACCACCTGGCATGAGGGAGAGGGGCTGAGGGATGGACTTCAGCCCCTCT
GCTCTGTACCCTTTTTCCTTTTGTCCCCGGCAGCAGGGAAGAAGCTGGAGGCCGTGGGAG
AATGGCTGGGCAGAGCAGAGGGGCAGCGATAGACTCTGGGGATGGAGCAGGACGGGGACG
GGAGGGGCCGGCCACCTGTCTGTAAGGAGACTTTGTTGCTTCCCCTGCCCCCGGAATCCA
CAGTGCTCTGCTTCTCTGTGTCGCCCCGCCCAGCCCCCTGGTGTGGAGGGAGGGGTCTCG
TTTGTGCGCGTGGGTGTAGCTTTGTGCATCCTCTCCCAGTGGAGCGATCACCTGTGCCTC
CCCTCCCCCTTTGTTTGCCCCTGTGTGGTTGGTCAAGGAGGGATGTGAGGGAAATAGGGA
CCCCCCGACTTGCCCTCCTGCCTCAGTCTTTCCCCCACCCTGTCTCTTCCTTGTCCTTCT
CTGGAAAATGCCAAAATACACGATGTGAATAAAAGTACAACGGCTAAAAAAAAAAAAAAA
AAAC
>OriGene 5' read for NM_003481 unedited
ATATTTGTATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGNAGAAGCTGCTGC
CGGTGTCATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGT
CCCTAAGGCTGGAGACCGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGA
GTCTGAGGGGGGCCTCTACATCTGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGT
GGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCG
CCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCG
GCTGGCTATTGGTGTTGAAGGCGGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGA
GGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGG
ACTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGA
CTCAGCCTCCCGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAA
GCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGNGCTGGA
AGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCC
TCTGTGGGCGACGCTACTTCGATGGCAGTGGNGGCAACACCACGCTGTGGAGCACTACCG
AGAGACGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGGAGCTGACGT
GTACTNCATATGATGAGGATTGACATGGGTCCCTGGACNCCCAGCCTGGNCTGAGCACCC
TGTCCACTTNNCGCATCGACATGGCTGAAGAATGCNNAAGACAGACAAGAACGATGACTG
AGNTGGNAGAAGACATGACCAGCNGNATNNNGTGATGGNGNAGCTGACN
>OriGene 3' read for NM_003481 unedited
CTATGGACCGCGCGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTAGCCGTTGTA
CTTTTATTCACATCGTGTATTTTGGCATTTTCCAGAGAAGGACAAGGAAGAGACAGGGTG
GGGGAAAGACTGAGGCAGGAGGGCAAGTCGGGGGGTCCCTATTTCCCTCACATCCCTCCT
TGACCAACCACACAGGGGCAAACAAAGGGGGAGGGGAGGCACAGGTGATCGCTCCACTGG
GAGAGGATGCACAAAGCTACACCCACGCGCACAAACGAGACCCCTCCCTCCACACCAGGG
GGCTGGGCGGGGCGACACAGAGAAGCAGAGCACTGTGGATTCCGGGGGCAGGGGAAGCAA
CAAAGTCTCCTTACAGACAGGTGGCCGGCCCCTCCCGTCCCCGTCCTGCTCCATCCCCAG
AGTCTATCGCTGCCCCTCTGCTCTGCCCAGCCATTCTCCCACGGCCTCCAGCTTCTTCCC
TGCTGCCGGGGACAAAAGGAAAAAGGGTACAGAGCAGAGGGGCTGAAGTCCATCCCTCAG
CCCCTCTCCCTCATGCCAGGTGGTCTTCCCCTGCCCTCATTGGTAAGGGGTGAGGCAGGC
TCTTAGCTGGCCACTCTCTGGTAGAAGTAGATGTAGCCCAGGTCCTTGGGCGGCTTCTCG
GAGGCACACACTTTCTGGTCATTGTAGATCACCCATCTGCCTTCTTTCTTGATGTGGCAG
ACGTAGTGACCACACATGGTAGAGGTGCCCATGTGACTAATGAAGGCAAAGAGCTGATAC
TTTNCAGGACCATCCCGGACTTTANGTCCCACTGGCACAGACTCANAGATGGAGTCGGCA
GCTGAGCGGCCTCTGAGATGTCATGCAGCTTCACATCCAGTCGTCATGTGACTGAAGATC
ATCCCAGC
Restriction Sites NotI-NotI     
ACCN NM_003481
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003481.1, NP_003472.1
RefSeq Size 3102 bp
RefSeq ORF 2508 bp
Locus ID 8078
UniProt ID P45974
Domains UBA, UCH, zf-UBP
Protein Families Druggable Genome, Protease
Gene Summary Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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