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Vinculin (VCL) (NM_003373) Human Untagged Clone
CAT＃: SC117985
VCL (untagged)-Human vinculin (VCL), transcript variant 2
CNY 11,144.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMD1W; CMH15; HEL114; MV; MVCL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117985 sequence for NM_003373 edited (data generated by NextGen Sequencing)
ATGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGCAGATC TCCCACCTGGTGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTC ACCGCGCCCGTGGCCGCCGTGCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAG ACTGTTCAAACCACTGAGGATCAGATTTTGAAGAGAGATATGCCACCAGCATTTATTAAG GTTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGATGCTTCAGTCAGACCCTTAC TCAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTCTGGAACATCA GACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCAAAGGA ATTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCACTTAC ACAAAGAATCTTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGACAGCAG GAGCTCACTCACCAGGAGCACCGAGTGATGTTGGTGAACTCGATGAACACCGTGAAAGAG TTGCTGCCAGTTCTCATTTCAGCTATGAAGATTTTTGTAACAACTAAAAACTCAAAAAAC CAAGGCATAGAGGAAGCTTTAAAAAATCGCAATTTTACTGTAGAAAAAATGAGTGCTGAA ATTAATGAGATAATTCGTGTGTTACAACTCACCTCTTGGGATGAAGATGCCTGGGCCAGC AAGGACACTGAAGCCATGAAGAGAGCATTGGCCTCCATAGACTCCAAACTGAACCAGGCC AAAGGTTGGCTCCGTGACCCTAGTGCCTCCCCAGGGGATGCTGGTGAGCAGGCCATCAGA CAGATCTTAGATGAAGCTGGAAAAGTTGGTGAACTCTGTGCAGGCAAAGAACGCAGGGAG ATTCTGGGAACTTGCAAAATGCTAGGGCAGATGACTGATCAAGTGGCTGACCTCCGTGCC AGAGGACAAGGATCCTCACCGGTGGCCATGCAGAAAGCTCAGCAGGTATCTCAGGGTCTG GATGTGCTCACAGCAAAAGTGGAAAATGCAGCTCGCAAGCTGGAAGCCATGACCAACTCA AAGCAGAGCATTGCAAAGAAGATCGATGCTGCTCAGAACTGGCTTGCAGATCCAAATGGT GGACCGGAAGGAGAAGAGCAGATTCGAGGTGCTTTGGCTGAAGCTCGGAAAATAGCAGAA TTATGTGATGATCCTAAAGAAAGAGATGACATTCTACGTTCCCTTGGGGAAATATCTGCT CTGACTTCTAAATTAGCAGATCTACGAAGACAGGGGAAAGGAGATTCTCCAGAGGCTCGA GCCTTGGCCAAACAGGTGGCCACGGCCCTGCAGAACCTGCAGACCAAAACCAACCGGGCT GTGGCCAACAGCAGACCGGCCAAAGCAGCTGTACACCTTGAGGGCAAGATTGAGCAAGCA CAGCGGTGGATTGATAATCCCACAGTGGATGACCGTGGAGTCGGTCAGGCTGCCATCCGG GGGCTTGTGGCCGAAGGGCATCGTCTGGCTAATGTTATGATGGGGCCTTATCGGCAAGAT CTTCTCGCCAAGTGTGACCGAGTGGACCAGCTGACAGCCCAGCTGGCTGACCTGGCTGCC AGAGGGGAAGGGGAGAGTCCTCAGGCACGAGCACTTGCATCTCAGCTCCAAGACTCCTTA AAGGATCTAAAAGCTCGGATGCAGGAGGCCATGACTCAGGAAGTGTCAGATGTTTTCAGC GATACCACAACTCCCATCAAGCTGTTGGCAGTGGCAGCCACGGCGCCTCCTGATGCGCCT AACAGGGAAGAGGTATTTGATGAGAGGGCAGCTAACTTTGAAAACCATTCAGGAAAGCTT GGTGCTACGGCCGAGAAGGCGGCTGCGGTTGGTACTGCTAATAAATCAACAGTGGAAGGC ATTCAGGCCTCAGTGAAGACGGCCCGAGAACTCACACCCCAGGTGGTCTCGGCTGCTCGT ATCTTACTTAGGAACCCTGGAAATCAAGCTGCTTATGAACATTTTGAGACCATGAAGAAC CAGTGGATCGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAAGCCATTGATACCAAA TCTCTGTTGGATGCTTCAGAAGAAGCAATTAAAAAAGACCTGGACAAGTGCAAGGTAGCT ATGGCCAACATTCAGCCTCAGATGCTGGTTGCTGGGGCAACCAGTATTGCTCGTCGGGCC AACCGGATCCTGCTGGTGGCTAAGAGGGAGGTGGAGAATTCCGAGGATCCCAAGTTCCGT GAGGCTGTGAAAGCTGCCTCTGATGAATTGAGCAAAACCATCTCCCCAATGGTGATGGAT GCAAAAGCTGTGGCTGGAAACATTTCCGACCCTGGACTGCAAAAGAGCTTCCTGGACTCA GGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT GACTTCCCGCCGCCTCCACCAGACCTTGAACAACTCCGACTAACAGATGAGCTTGCTCCT CCCAAACCACCTCTGCCTGAAGGTGAGGTCCCTCCACCTAGGCCTCCACCACCAGAGGAA AAGGATGAAGAGTTCCCTGAGCAGAAGGCCGGGGAGGTGATTAACCAGCCAATGATGATG GCTGCCAGACAGCTCCATGATGAAGCTCGCAAATGGTCCAGCAAGGGCAATGACATCATT GCAGCAGCCAAGCGCATGGCTCTGCTGATGGCTGAGATGTCTCGGCTGGTAAGAGGGGGC AGTGGTACCAAGCGGGCACTCATTCAGTGTGCCAAGGACATCGCCAAGGCCTCAGATGAG GTGACTCGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAACGGATTAGAACCAAC CTCTTACAGGTATGTGAGCGAATCCCAACCATAAGCACCCAGCTCAAAATCCTGTCCACA GTGAAGGCCACCATGCTGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACA GAGATGCTGGTTCACAATGCCCAGAACCTCATGCAGTCTGTGAAGGAGACTGTGCGGGAA GCTGAAGCTGCTTCAATCAAAATTCGAACAGATGCTGGATTTACACTGCGCTGGGTTAGA AAGACTCCCTGGTACCAGTAG Clone variation with respect to NM_003373.3 2388 g=>a >OriGene 5' read for NM_003373 unedited
AGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCTCTTCGCC GGTTCCCGGCCCCGTGGATCCTACTTCTCTGTCGCCCGCGGTTCGCCGCCCCGCTCGCCG CCGCGATGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGC AGATCTCCCACCTGGTGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTG ACCTCACCGCGCCCGTGGCCGCCGTGCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAA AAGAGACTGTTCAAACCACTGAGGATCAGATTTTGAAGAGAGATATGCCACCAGCATTTA TTAAGGTTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGATGCTTCAGTCAGACC CTTACTCAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTCTGGAA CATCAGACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCA AAGGAATTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCA CTTACACAAAGAATCTTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGAC AGCAGGAGCTCACTCACCAGGAGCACCGAGTGATGTTGGTGAACTCGATGAACACCGTGA AAGAGTTGCTGCCAGTTCTCATTTCAGCTATGAAGAATTTTTGTTACACTAAAAACTCAN AAACCAAGGCATAGAGGAAGCTTTANAAATCGCAATTTTACTGTAGAANAATGAGTGCTG AAATAATGAGATATTTCGTGTGTACAACTCACCTCTTGGGATGAAGATGCCTGGNCCAGC AAGACACTGTAGCCATGGAGAGAGCATTGGNCTNCATAGACTCCAAACTGGACCG >OriGene 3' read for NM_003373 unedited
CTATGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTAATTTGCTT CACTGTGTCTCGAGCACTGATATTGGGAGAAAAGCACATCCGGCATAAAGTGTAAACCAG TGTCTCAAACCACTGGAAGAACCGGGAGAGCAAACATGATTTTTCTTATTTCCTCTAAGT AATCTTTCTTTAGTAAAACAACAAGTGATCTTTGGCATAGATTCATACTTTAAAGGCATT AATATTGCATTTATATCAGGCAAGCAACTATACAAATATGCTGAGGGCCTTGAAAATAAT CATCCTCATTTTAAAAGAAATAGTGAAAGCCTGAGTGTAAAGGACCAACTTAAGTTGTAC ACATTCGATGTTGGGAACTAACACACAGCGATGGGTGGGAAGGAAGGATGTTCAGGCAAG GTTCTTACTCCTTTACTCATCTGGTTCTGGCTTTGGGAAAAAATAAGGTTTCATGTGCTG GGAAATACTTAGCAGTAATAAGTACCAAAAAGGAAACACTGCCCTCTCATTTTGCCTAGT AGGAACTTACTGTGGTGATAAGAAATATGAAACCCATTACTCTCTTGAACCCCATACTTG GGAGTAGATGCAGAGAGCTTGGTTGTAGTTGGTTGCAGGGAGGTAGGAATTACAGGAGTA GTATTTCCCATTGATTCATCTTCAGCAGCACTAGAATTCTAGTCTGCAAATACCACTCTC CCTTAGGCAGGAAGTGTCCTTCAGACAGGGCTGCCTTGCCATTAAACACTCTTNAGGGAC AAGCACTCCTCTTTCTTTAGACTGAATTAAAAAGGATCTTTTAGAGTTACAGATGAACAN ACACCTACATGTCTTTTAAAATCTTGTTANAAAGGGATGTGGAGGAGGGCTACAAAACCA GNATATAGGAGACTGGTNTGGTTAAAATAAAATGCAGCATTTCAGCTCANATGGACCATT AGAGCCTAGATGAATGATAGGCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003373 |
| Insert Size | 4840 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003373.3, NP_003364.1 |
| RefSeq Size | 5443 bp |
| RefSeq ORF | 3201 bp |
| Locus ID | 7414 |
| UniProt ID | P18206 |
| Domains | Vinculin |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| Gene Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
,Yu, D;Baird, MA;Allen, JR;Howe, ES;Klassen, MP;Reade, A;Makhijani, K;Song, Y;Liu, S;Murthy, Z;Zhang, SQ;Weiner, OD;Kornberg, TB;Jan, YN;Davidson, MW;Shu, X;,
Nat. Methods
,PubMed ID 26098020
[VCL]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204576 | VCL (Myc-DDK-tagged)-Human vinculin (VCL), transcript variant 2 |
CNY 11,136.00 |
|
| RC204576L1 | Lenti ORF clone of Human vinculin (VCL), transcript variant 2, Myc-DDK-tagged |
CNY 13,536.00 |
|
| RC204576L2 | Lenti ORF clone of Human vinculin (VCL), transcript variant 2, mGFP tagged |
CNY 13,536.00 |
|
| RC204576L3 | Lenti ORF clone of Human vinculin (VCL), transcript variant 2, Myc-DDK-tagged |
CNY 11,020.00 |
|
| RC204576L4 | Lenti ORF clone of Human vinculin (VCL), transcript variant 2, mGFP tagged |
CNY 11,020.00 |
|
| RG204576 | VCL (tGFP-tagged) - Human vinculin (VCL), transcript variant 2 |
CNY 12,736.00 |
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