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WEE1 (NM_003390) Human Untagged Clone
CAT＃: SC117999
WEE1 (untagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1
CNY 4,816.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | WEE1A; WEE1hu |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003390 edited
ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCACCCCGCCGCGCCGGGGCGGCCTGCACC TTGCGGCAGAAGCTGATCTTCTCGCCCTGCAGCGACTGTGAGGAGGAGGAAGAAGAGGAG GAGGAGGAGGGCAGCGGCCACAGCACCGGGGAGGACTCGGCCTTTCAAGAGCCCGACTCG CCGCTGCCGCCCGCGCGGAGCCCCACGGAGCCCGGGCCCGAGCGCCGCCGCTCGCCCGGG CCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGGACCTGTTGCTGCCCGGCGCCTGCCCG GGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCGACTCGTGGGAGGAGGAGGGCTTCGGC TCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCTACTTCCTGGGTAGCTCTTTCTCGCCG GTGCGCTGCGGCGGCCCAGGAGATGCGTCGCCGCGGGGTTGCGGGGCGCGCCGGGCGGGC GAAGGCCGCCGCTCGCCGCGGCCGGACCACCCGGGCACCCCGCCACACAAGACCTTCCGC AAGCTGCGACTCTTCGACACCCCGCACACGCCCAAGAGTTTGCTCTCCAAAGCTCGGGGA ATTGATTCCAGCTCTGTTAAACTCCGGGGTAGTTCTCTCTTCATGGATACAGAAAAATCA GGAAAAAGGGAATTTGATGTGCGACAGACTCCTCAAGTGAATATTAATCCTTTTACTCCG GATTCTTTGTTGCTTCATTCCTCAGGACAGTGTCGTCGTAGAAAGAGAACGTATTGGAAT GATTCCTGTGGTGAAGACATGGAAGCCAGTGATTATGAGCTTGAAGATGAAACAAGACCT GCTAAGAGAATTACAATTACTGAAAGCAATATGAAGTCCCGGTATACAACAGAATTTCAT GAGCTAGAGAAAATCGGCTCTGGAGAATTTGGTTCTGTATTTAAGTGTGTGAAGAGGCTG GATGGATGCATTTATGCCATTAAGCGATCAAAAAAGCCATTGGCGGGCTCTGTTGATGAG CAGAACGCTTTGAGAGAAGTATATGCTCATGCAGTGCTTGGACAGCATTCTCATGTAGTT CGATATTTCTCTGCGTGGGCAGAAGATGATCATATGCTTATACAGAATGAATATTGTAAT GGTGGAAGTTTAGCTGATGCTATAAGTGAAAACTACAGAATCATGAGTTACTTTAAAGAA GCAGAGTTGAAGGATCTCCTTTTGCAAGTTGGCCGAGGCTTGAGGTATATTCATTCAATG TCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTCATATCTCGAACCTCAATCCCA AATGCTGCCTCTGAAGAAGGAGACGAAGATGATTGGGCATCCAACAAAGTTATGTTTAAA ATAGGTGATCTTGGGCATGTAACAAGGATCTCCAGTCCACAAGTTGAAGAGGGCGATAGT CGTTTTCTTGCAAATGAAGTTTTACAGGAGAATTATACCCATCTACCAAAAGCAGATATT TTTGCGCTTGCCCTCACAGTGGTATGTGCTGCTGGTGCTGAACCTCTTCCGAGAAATGGA GATCAATGGCATGAAATCAGACAGGGTAGATTACCTCGGATACCACAAGTGCTTTCCCAA GAATTTACAGAGTTGCTAAAAGTTATGATTCATCCAGATCCAGAGAGAAGACCTTCAGCA ATGGCACTGGTAAAGCATTCAGTATTGCTGTCCGCTTCTAGAAAGAGTGCAGAACAATTA CGAATAGAATTGAATGCCGAAAAGTTCAAAAATTCACTTTTACAAAAAGAACTCAAGAAA GCACAGATGGCAAAAGCTGCAGCTGAGGAAAGAGCACTCTTCACTGACCGGATGGCCACT AGGTCCACCACCCAGAGTAATAGAACATCTCGACTTATTGGAAAGAAAATGAACCGCTCT GTCAGCCTTACTATATACTGA >OriGene 5' read for NM_003390 unedited
GATAGCGCTGCGGTNGAAAACGAATCCGGGGGGCGGAAAAACCTTCGCACGGGGCCGCCC CGTTTCTGCCGGAAGTCCGCGCCGCCGCTGCCGCCACCGTCCGCAGCCCGAGCGCCCCGG AGCCGCAGGCCGCCGCCGCGCAGAGACGCCGCGGCTGCGACTAGGCGCGCCCAGCCGCAC GTGGCGGACCCGCCCCCAGGCCCGCAGTGTTTTTTTATTCCGCAGGCCTCCGCTCTCCTG TCCTCGGCCCCGTCCCCAGGGCCGCGATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCAC CCCGCCGCGCCGGGGCGGCCTGCACCTTGCGGCAGAAGCTGATCTTCTCGCCCTGCAGCG ACTGTGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGGGCAGCGGCCACAGCACCGGGGAGG ACTCGGCCTTTCAAGAGCCCGACTCGCCGCTGCCGCCCGCGCGGAGCCCCACGGAGCCCG GGCCCGAGCGCCGCCGCTCGCCCGGGCCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGG ACCTGTTGCTGCCCGGCGCCTGCCCGGGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCG ACTCGTGGGAGGAGGAGGGCTTCGGCTCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCT ACTTCCTGGGGAGCTCTTTCTCGCCGGTGCGCTGCGGCGGCCCAGGAGATGCCTCCCCGC GGGGTTGCGGGAGCGCACGGAGGAGCATGATCCTCTCGCGACGCTGGGAGTGATACGGCG GGGGGAGGGAGGGGGAGGAGCAGGCATGAGGGAGAGGTCGGGCGGGCGAGGCCCGGGGGC AGGAGGGAGTGGAGGGGGGGGGGGAGGGAGGGAGGGTGGGGTGGTGGGG >OriGene 3' read for NM_003390 unedited
TGGACCGCGGCACGCAATCTAGTAGTCAGNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTAAAACCCAAAGAGACTTTTAATATGCCAATCAATGTTAATAAAACACAAG TCAAAAACAAGGGCAAACATGTTTTAAACCAAATTTAATGGAAAAAACAAACAGACATTT TTTTTCAACATCTGTTAGCCAGTATTATTAGTCAAATGGCTAATCACAAATAAAATATAT TTTGGGAAAAACTTGGAATGGCAAAAGTCATTCTGGCATTTCAAACAGCTATGTACAGTA TCACAAAAATCGGTTTATATACACAAATATTGAAAAAAAAAACCGGGCAAAACATTTAAA AACAGACTAATAATACAATCAAGTTTAAAACTTAGGGGGAAACATTTAAAAAGAGTCCGG GAAGGACATTACCAAAACAAGTTACAAAACCAGGAATTATTATACTACATTAATACAGCA TTCCAAAGGAGGGAAAGGGGGCATTTTTCCAATGCACTAGCTTCAAAGTTCAAAGGAAAA AAAAAAAAAGGCAAATAGCTGCTTTTCATCAAAAGCAGCTATACATTTCACATAATTGTT TCAAAAAGAAGACCTATTAACAACAATGATTGTTTAATGCTACAAGTTTACAGCAAAGAC AAAACTAAAATAGCAGCAAATTCACAAGGCATTACCTCCACAGAATCATCATCCCATGGT TCATGCAATGCCTACAAAGGGCTCCCAGTGGGATATACAAGTCTAATTCCCATTTAAAAA AAAAGGATATATAGATGCACACCCAGGTCCATTGAGGCTGAGTAGTAATGAGTCTTTAGC ATGTCCCTTTTAAAGAAGGGTATGTCTGAATAAAGCCCTGATACTTTTTCAGGAACTACA GGAAAGAACCTTTTTTACATGCACAGCAACTAAGCGCCCCCCCCCGGGGTAACCTGAAAG CTGGGTACTCCACAGGGCACTCTAAAAGAAATGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003390 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003390.2, NP_003381.1 |
| RefSeq Size | 4232 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 7465 |
| UniProt ID | P30291 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle |
| Gene Summary | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Bifunctional Regulatory Element in Human Somatic Wee1 Mediates Cyclin A/Cdk2 Binding and Crm1-Dependent Nuclear Export
,Changqing Li, Mark Andrake, Roland Dunbrack, and Greg H. Enders,
Mol. Cell. Biol., Jan 2010; 30: 116 - 130
[WEE1]
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A bi-functional regulatory element in human somatic Wee1 mediates cyclin A/Cdk2 binding and Crm1-dependent nuclear export
,Changqing Li, Mark Andrake, Roland Dunbrack, and Greg H. Enders,
Mol. Cell. Biol., Oct 2009; 10.1128/MCB.01876-08
[WEE1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209760 | WEE1 (Myc-DDK-tagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 |
CNY 4,808.00 |
|
| RC209760L1 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
| RC209760L2 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209760L3 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
| RC209760L4 | Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged |
CNY 7,208.00 |
|
| RG209760 | WEE1 (tGFP-tagged) - Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 |
CNY 6,408.00 |
|
| SC323510 | WEE1 (untagged)-Kinase deficient mutant (K328M) of Human WEE1 homolog (S. pombe) (WEE1) |
CNY 4,816.00 |
