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Calprotectin (S100A8) (NM_002964) Human Untagged Clone
CAT＃: SC118302
S100A8 (untagged)-Human S100 calcium binding protein A8 (S100A8)
CNY 1,800.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118302 sequence for NM_002964 edited (data generated by NextGen Sequencing)
ATGTTGACCGAGCTGGAGAAAGCCTTGAACTCTATCATCGACGTCTACCACAAGTACTCC CTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGAAGAAATTGCTAGAGACC GAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGGTTCAAAGAGTTGGATATC AACACTGATGGTGCAGTTAACTTCCAGGAGTTCCTCATTCTGGTGATAAAGATGGGCGTG GCAGCCCACAAAAAAAGCCATGAAGAAAGCCACAAAGAGTAG Clone variation with respect to NM_002964.4 >OriGene 5' read for NM_002964 unedited
ACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAAGACCTGGTGGG GCAAGTCCGTGGGCATCATGTTGACCGAGCTGGAGAAAGCCTTGAACTCTATCATCGACG TCTACCACAAGTACTCCCTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGA AGAAATTGCTAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGGT TCAAAGAGTTGGATATCAACACTGATGGTGCAGTTAACTTCCAGGAGTTCCTCATTCTGG TGATAAAGATGGGCGTGGCAGCCCACAAAAAAAGCCATGAAGAAAGCCACAAAGAGTAGC TGAGTTACTGGGCCCAGAGGCTGGGCCCCTGGACATGTACCTGCAGAATAATAAAGTCAT CAATACCTCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTG TTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCC TGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTT TGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGTGGTATGGAGCAGGGG CAAGTTGGGAAGACAACCTGTAGGGCCTGCGGNGTCTATTGGGAACCAAGCTGGAGTGCA GTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCT CAGCCTCCCGAGTTGTTGGGAATTCCAGCATGCATGNACCAGCTCAGCTAATTTTTGGTT TTTTGGTAGAAACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCACTCCTAATCTCAGT GATCTACCCACCTTGCCTCCAANTGCTGGATTACAGCGTGACACTGCTCCTTCTGTCTCT GATTTAAAAC >OriGene 3' read for NM_002964 unedited
GGCCCCCGGCCCANACAANNNCCCCTCANNNNNNAAGGTTTTGACTTGNNACCGCGGCCG CATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTNGAGTATTGATGACTTTATTATTCTGC AGGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCTTT CTTCATGGCTTTTTTTGTGGGCTGCCACGCCCATCTTTATCACCAGAATGAGGAACTCCT GGAAGTTAACTGCACCATCAGTGTTGATATCCAACTCTTTGAACCAGACGTCTGCACCCT TTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGA CGGCATGGAAATTCCCCTTTATCAGGGAGTACTTGTGGTAGACGTCGATGATAGAGTTCA AGGCTTTCTCCAGCTCGGTCAACATGATGCCCACGGACTTGCCCCACCAGGTCTTCCTCG TGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAA CGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGN GCGGGGTTATTACGACATTNTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCA TTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCA TTGGGTGTACTGCCAAACCGCATCACCATGGTAATAGCGATGACTATACGTANATGTACT GCCAAGTANGAAAGTCCCCGTAAGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTAC CGTCATTGACGTTCATAGGGGGCCGGACTTGGCATATGATACACTCGATGAACTGGCAAG GGGGCAGTTTACCGGAAAACCTCCCCCATTGACGGCCATGGAAAAGCCCTTATTGGGTTA CTTTGGGAACTACGTACTTTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002964 |
| Insert Size | 450 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002964.3, NP_002955.2 |
| RefSeq Size | 428 bp |
| RefSeq ORF | 282 bp |
| Locus ID | 6279 |
| UniProt ID | P05109 |
| Domains | S_100, EFh |
| Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (4) uses an alternate splice junction compared to variant 1. The resulting isoform (d) is shorter at the N-terminus compared to isoform a. Variants 4 and 5 both encode the same isoform (d). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203255 | S100A8 (Myc-DDK-tagged)-Human S100 calcium binding protein A8 (S100A8) |
CNY 1,800.00 |
|
| RC203255L1 | Lenti ORF clone of Human S100 calcium binding protein A8 (S100A8), Myc-DDK-tagged |
CNY 4,200.00 |
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| RC203255L2 | Lenti ORF clone of Human S100 calcium binding protein A8 (S100A8), mGFP tagged |
CNY 4,200.00 |
|
| RC203255L3 | Lenti ORF clone of Human S100 calcium binding protein A8 (S100A8), Myc-DDK-tagged |
CNY 6,840.00 |
|
| RC203255L4 | Lenti ORF clone of Human S100 calcium binding protein A8 (S100A8), mGFP tagged |
CNY 4,200.00 |
|
| RG203255 | S100A8 (tGFP-tagged) - Human S100 calcium binding protein A8 (S100A8) |
CNY 3,400.00 |
