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RASA1 (NM_002890) Human Untagged Clone
CAT＃: SC118332
RASA1 (untagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1
CNY 7,296.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118332 sequence for NM_002890 edited (data generated by NextGen Sequencing)
ATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCGGCCCGGTAACAGCCGGAGCTGGAGGA GGCGGCGCGGCAGCGGGCTCCAGTGCCTATCCCGCAGTGTGTCGGGTGAAGATACCCGCG GCCCTGCCTGTGGCAGCCGCCCCCTATCCTGGGCTGGTGGAGACCGGAGTGGCTGGAACT CTGGGTGGCGGAGCCGCTTTGGGGTCAGAGTTCCTAGGAGCCGGGTCTGTGGCAGGGGCA CTGGGGGGAGCTGGACTGACAGGGGGAGGTACTGCTGCTGGCGTAGCTGGTGCTGCTGCT GGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTGGAGACATGGCTCTCACCAAACTGCCC ACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCGGCGGTTTTCCCCCTCTGCCCCCTCCC CCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGACAGTGGACGAAGGTGACTCTCTGGAT GGACCAGAATACGAGGAGGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAG TGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAG TCTGGCAGTTATCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCATTT CTTAGCCAGATGAATGTTGTCAACCATTTTAGGATTATTGCTATGTGTGGAGATTACTAC ATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAGTCATGTTTCT TGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCAGTAGAAGAT AGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGATGAAATAAGT TTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGGATGTGGGTT ACAAATTTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGC CGGGAAGAAGATCCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAA GCTTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGGCCCTCAGAT AATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAAAATATTCAGCGATTTAAA ATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGGTATTATAACAGCATTGGG GACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTTAAGGAACCT GTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTAT AATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTAT CTTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGTAGT GATGCCCAACTTATTTATTTTGAAAGCGAAAAACGAGCTACCAAACCAAAAGGATTAATA GATCTCAGTGTATGTTCTGTCTATGTCGTTCATGATAGTCTCTTTGGCAGGCCAAACTGT TTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAA ACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAATTTACGGAAA AGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTTTTACATATT GAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAACATCTACCTG AATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAA GAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAAT AAAACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTTGAGCCGATTA CAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCCCATATACCATTAAAAGGT ATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTATGGAAAAAATCATGCCAGAA GAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTAGTCTATGCT TTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTT CTTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAATAAGCATG GAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTGATGGAGCAG TATATGAAAGCCACTGCTACACAGTTTGTTCATCATGCTTTGAAAGACTCTATTTTAAAG ATAATGGAAAGCAAGCAGTCTTGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGAT GTGAACACTAATTTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAGAAAATATTC ATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTACAGAAATCT GTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGTGGTTTTGTT TTTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATCATCTCAGAT TCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTA GCAAATCTTGTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATC AAAAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTT CCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGATTTAGCAGCATTGCATGAG ATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGTGCACAGCAG CACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAAAAACAAAACCAGTAT ACAAAAACCAATGATGTCAGGTAG Clone variation with respect to NM_002890.2 >OriGene 5' read for NM_002890 unedited
CAGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCGCGGC GGCGGGCTCTCTCCTTTTGTTGTTGTTTCCTCAGCCTGGGGAGCTGAAGGGGAGACGCGT CTGGGTGGGGCTGCTCGGAGCCCGGGCCTGGTGGCCCCTGGGGCTCCCGGGCGGGCAGGG TAGGGCAGAGTAGAGCGGGCTTCAACATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCG GCCCGGTAACAGCCGGAGCTGGAGGAGGCGGCGCGGCAGCGGGCTCCAGTGCCTATCCCG CAGTGTGTCGGGTGAAGATACCCGCGGCCCTGCCTGTGGCAGCCGCCCCCTATCCTGGGC TGGTGGAGACCGGAGTGGCTGGAACTCTGGGTGGCGGAGCCGCTTTGGGGTCAGAGTTCC TAGGAGCCGGGTCTGTGGCAGGGGCACTGGGGGGAGCTGGACTGACAGGGGGAGGTACTG CTGCTGGCGTAGCTGGTGCTGCTGCTGGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTG GAGACATGGCTCTCACCAAACTGCCCACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCG GCGGTTTTCCCCCTCTGCCCCCTCCCCCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGA CAGTGGACGAAGGTGACTCTCTGGATGGACCAGAATACGAGGAGGAAGAGGTGGCCATAC CGTTGACCGCTCCTCCAACTAACCAGTGGTATCACGGAAAACTTGACAGAACGATAGCAG AAGAACGCCTCANGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGATCGGG AGCCAGGGTCCTTTGTACTTTCATTTCTAGCCAGATGAATGGTGTCAACCATTTTAGGAT ATTGCTATGTGTGGAGATACTACATGGGTGGAAGACGNTNTC >OriGene 3' read for NM_002890 unedited
GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAGATAT CCCTTGGTTTTATTTTAACTTAACCTTTGTGAATACTTCACAAAAATACACATACAGAAG TAAATAAAACTATACAGTTGAACTAAATATCCAGCAGATACAACAAGTAGTCAGAAGTTC AAAGATGGATAAGTTAGGCAGCTGATGAGGTTGTCTGCATAATCAAAGGGGTAGCACAAA TAATGAACAAATAACTTTTAACAAAAGCATTCTAGTTAACATAAAAGAAATTGGTAGTCT ATAAAATGGTTGAATGGGTAACATGCATTGATCAATACAACAACCAATAATATTTACTGA ATTCAAACTATTTACTTGTAAACATTTAACAAAGCATTATGAGATCTAAATACAGTCTTT GACAATTTCAACAAAATAAGTCGTATGTTTAAAAAATAAAGCTGATGAGCAATAGATCAT TCTATATATTTTCTTTCAACAGTTCCAGCAAGAGAAATAGCAGAGTATTTTTTAAAAGTA TACAGCAAAGATTGTTCCTATTATAATGACAAACTCTGGAAACTGTAAATACAAATAATC ATTGAACAGTCAAATGGTCATATTCCTAATCAGGAAGTAGTTACAGTAAGAACAGTCTGC AATCCAGTTATACACAATTGTAATAAGAATGTGTTTCTTGTCAGNTGATTTCATAGTTCT TTCTCTAAGAGACAGCAAACTTGGCACTGGGTTGACTGAAATATATTTTGAAGGTCGAGA ATCCAGAACAGTCTAGCATTTCGTTTGGGAAAGGGGCCTGACACCAGCTAAAAGGTGTTA AGATGAGTCCTGGGGAATAGGAAATTTCTGCCAGAAGCTACAAGGTGCTCCAAGTTATAC CACCAATTTGGGATTTGTTGCAAGTCACTCCTGATGGGGGAAAGGGCTTTTTGGGAGACA GGGAATAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002890 |
| Insert Size | 4230 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002890.1, NP_002881.1 |
| RefSeq Size | 4307 bp |
| RefSeq ORF | 3144 bp |
| Locus ID | 5921 |
| UniProt ID | P20936 |
| Domains | C2, SH2, SH3, PH, RasGAP |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012] Transcript Variant: This variant (1) lacks the alternatively spliced insert found in variant 2, resulting in translation of the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inactivation of RASA1 promotes melanoma tumorigenesis via R-Ras activation
,Sung, H;Kanchi, KL;Wang, X;Hill, KS;Messina, JL;Lee, JH;Kim, Y;Dees, ND;Ding, L;Teer, JK;Yang, S;Sarnaik, AA;Sondak, VK;Mulé, JJ;Wilson, RK;Weber, JS;Kim, M;,
Oncotarget
,PubMed ID 26993606
[RASA1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205473 | RASA1 (Myc-DDK-tagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1 |
CNY 7,288.00 |
|
| RC205473L1 | Lenti ORF clone of Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, Myc-DDK-tagged |
CNY 9,688.00 |
|
| RC205473L2 | Lenti ORF clone of Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, mGFP tagged |
CNY 10,830.00 |
|
| RC205473L3 | Lenti ORF clone of Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, Myc-DDK-tagged |
CNY 9,688.00 |
|
| RC205473L4 | Lenti ORF clone of Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, mGFP tagged |
CNY 9,688.00 |
|
| RG205473 | RASA1 (tGFP-tagged) - Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1 |
CNY 8,888.00 |
