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Glucosidase 2 subunit beta (PRKCSH) (NM_002743) Human Untagged Clone
CAT＃: SC118458
PRKCSH (untagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1
CNY 5,904.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGE-R2; G19P1; GIIB; PCLD; PCLD1; PKCSH; PLD1; VASAP-60 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118458 sequence for NM_002743 edited (data generated by NextGen Sequencing)
ATGCTGTTGCCGCTGCTGCTGCTGCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCC CGGGGCGTCTCCCTCACCAATCATCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTG GACGGTTCGGCCACCATCCCATTTGATCAGGTCAACGATGACTATTGCGACTGCAAAGAT GGCTCTGACGAGCCAGGCACGGCTGCCTGTCCTAATGGCAGCTTCCACTGCACCAACACT GGCTATAAGCCCCTGTATATCCCCTCCAACCGGGTCAACGATGGTGTTTGTGACTGCTGC GATGGAACAGACGAGTACAACAGCGGCGTCATCTGTGAGAACACCTGCAAAGAGAAGGGC CGTAAGGAGAGAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTCCGTCTG AAGAAGATCCTTATTGAGGACTGGAAGAAGGCACGGGAGGAGAAGCAGAAAAAGCTCATT GAGCTACAGGCTGGGAAGAAGTCTCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAG GAGGAAGCTGAGAAGCCAGAGAGAGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAG CAGCTGGCTGCTGCCAAGGCCCAACAGGAGCAGGAGCTGGCGGCTGATGCCTTCAAGGAG CTGGATGATGACATGGACGGGACGGTCTCGGTGACTGAGCTGCAGACTCACCCGGAGCTG GACACAGATGGGGATGGGGCGTTGTCAGAAGCGGAAGCTCAGGCCCTCCTCAGTGGGGAC ACACAGACAGACGCCACCTCTTTCTACGACCGCGTCTGGGCCGCCATCAGGGACAAGTAC CGGTCCGAGGCACTGCCCACCGACCTTCCAGCACCTTCTGCCCCTGACTTGACGGAGCCC AAGGAGGAGCAGCCGCCAGTGCCCTCGTCGCCCACAGAGGAGGAGGAGGAGGAGGAGGAG GAGGAGGAAGAAGAGGCTGAAGAAGAGGAGGAGGAGGAGGATTCCGAGGAGGCCCCACCG CCACTGTCACCCCCGCAGCCGGCCAGCCCTGCTGAGGAAGACAAAATGCCGCCCTACGAC GAGCAGACGCAGGCCTTCATCGATGCTGCCCAGGAGGCCCGCAACAAGTTCGAGGAGGCC GAGCGGTCGCTGAAGGACATGGAGGAGTCCATCAGGAACCTGGAGCAAGAGATTTCTTTT GACTTTGGCCCCAACGGGGAGTTTGCTTACCTGTACAGCCAGTGCTACGAGCTCACCACC AACGAATACGTCTACCGCCTCTGCCCCTTCAAGCTTGTCTCGCAGAAACCCAAACTCGGG GGCTCTCCCACCAGCCTTGGCACCTGGGGCTCATGGATTGGCCCCGACCACGACAAGTTC AGTGCCATGAAGTATGAGCAAGGCACGGGCTGCTGGCAGGGCCCCAACCGCTCCACCACC GTGCGCCTCCTGTGCGGGAAAGAGACCATGGTGACCAGCACCACAGAGCCCAGTCGCTGC GAGTACCTCATGGAGCTGATGACGCCAGCCGCCTGCCCGGAGCCACCGCCTGAAGCACCC ACCGAAGACGACCATGACGAGCTCTAG Clone variation with respect to NM_002743.2 >OriGene 5' read for NM_002743 unedited
GTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGGTGGATACT GACCTTTGCTCCGGCCTCGTCGTGAAGACACAGCGCATCTCCCCGCTGTAGGCTTCCTCC CACAGAACCCGTTTCGGGCCTCAGAGCGTCTGGTGAGATGCTGTTGCCGCTGCTGCTGCT GCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCCCGGGGCGTCTCCCTCACCAATCA TCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTGGACGGTTCGGCCACCATCCCATT TGATCAGGTCAACGATGACTATTGCGACTGCAAAGATGGCTCTGACGAGCCAGGCACGGC TGCCTGTCCTAATGGCAGCTTCCACTGCACCAACACTGGCTATAAGCCCCTGTATATCCC CTCCAACCGGGTCAACGATGGTGTTTGTGACTGCTGCGATGGAACAGACGAGTACAACAG CGGCGTCATCTGTGAGAACACCTGCAAAGAGAAGGGCCGTAAGGAGAGAGAGTCCCTGCA GCAGATGGCCGAGGTCACCCGCGAAGGGTTCCGTCTGAAGAAGATCCTTATTGAGGACTG GAAGAAGGCACGGGAGGAGAAGCAGANAAAGCTCATTGAGCTACAGGCTGGGAAGAAGTC TCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAGGAGGAAGCTGAGAAGCCAGAGAG AGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAGCAGCTGGCTGCTGCCAAGGCCCA CAGGAGCANGAGCTGGCGGCTGATGCCTTTCAGGAGCTGNATGATGACATGGACGGNACN NGTCTCGTGACTGAGCTGCAGACTCACCCGNAGCTGGAACAGATGGNGATGGGNNGCGTG TCANAAGCGAAGCTCAGCCCTNCTCAGTGGGACACC >OriGene 3' read for NM_002743 unedited
GGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGGTGGGGGATCACATTTATTG TATTGAGGTCACAGGTCAAGTCATTCACTATTCCCCACTAAGAGGGGTGGCAAGGACAGG GCTGGGGGTGGTGGGGCGAGTCACCAAGGTGGGGGGCCCAGGGAGGGCAAGCTCCTTTAC CCATCTTTGAAGGCTGTTGGGGCCTGGGAATGTGGACCAGCCCCGCCTGGGCCCCATAGC AGGGCACGAATCCCCACAAAGGTCCTGCCGCCACAGATGATGGCAACAGATCCACAGGCA GGCCCATAGGGGCGGGTGGACGGCACTGCAAGGGCTGGCTTCATGCCTTCTTGAAGTTCT CTGCGCCCATCCAGCTAGAGCTCGTCATGGTCGTCTTCGGTGGGTGCTTCAAGCGGTGGC TCCGGGCAAGCGGCTGGCGTCATCAGCTCCATGAAGTACTCGCAGCGACTGGGCTCTGTG GTGCTGGTCACCATGGTCTCTTTCCCGCACAGGATGCGCACGGTGGTGGATCGGTTGGGG CCCTGCCAGCAGCCCGTGCCTTGCTCATACTTCATGGCACTGAACTTGTCGTGGTCGGGG CCAATCCATGATCCCCAAGTGCCAAAGCTGGTGGGAGATGCCCCGAGTTGGGTTTCTGCG ATCAATCTTGAAAGGGCAGAAGCCGTANAACTATTCGTTGGGTGTGAGCTCGTANCACTG GCTGTACAAGTAAGCAAACTCCCCGTTGGGCCAAAGCAAAAAAAATCCTTGCTCCAAGCT CCTGATGGACTCTTCATGTNCTTAATCGACCGCTCGGCCTCCTCAAACTTGGTGGGGCCC TCCTGGCAGAATATATAAAAGCCTGCCCTGCTCCTCTAGGGAGCATTTTTTTTTCTCACA AGGCTGGCCGTTGCGGGGTAAAATGCCGGTGGGCCCCTGGAATCCCCTCCCCCCCTTTTT AAACTCTCTTCTCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002743 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002743.2, NP_002734.2 |
| RefSeq Size | 2262 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 5589 |
| UniProt ID | P14314 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) encodes isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213274 | PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 6,288.00 |
|
| RC213274L3 | Lenti-ORF clone of PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 6,460.00 |
|
| RC213274L4 | Lenti-ORF clone of PRKCSH (mGFP-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 6,460.00 |
|
| RG213274 | PRKCSH (tGFP-tagged) - Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 7,888.00 |
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