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PCNA (NM_002592) Human Untagged Clone
CAT＃: SC118528
PCNA (untagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATLD2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118528 sequence for NM_002592 edited (data generated by NextGen Sequencing)
ATGTTCGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAG GACCTCATCAACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATG GACTCGTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTAC CGCTGCGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAA TGCGCCGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCG CTAGTATTTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGAT TTAGATGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCT TCTGGTGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATT TCCTGTGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGAAATGGAAACATT AAATTGTCACAGACAAGTAATGTCGATAAAGAGGAGGAAGCTGTTACCATAGAGATGAAT GAACCAGTTCAACTAACTTTTGCACTGAGGTACCTGAACTTCTTTACAAAAGCCACTCCA CTCTCTTCAACGGTGACACTCAGTATGTCTGCAGATGTACCCCTTGTTGTAGAGTATAAA ATTGCGGATATGGGACACTTAAAATACTACTTGGCTCCCAAGATCGAGGATGAAGAAGGA TCTTAG Clone variation with respect to NM_002592.2 >OriGene 5' read for NM_002592 unedited
NGGGTCAGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGCT TGCGGACGCGGCGGCATTAAACGGTTGCAGGACCAGCAGAGTGGTCGTTGTCTTTCTAGG TCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCTCTGAGGCTCCTGAAGCCGAAACCAG CTAGACTTTCCTCCTTCCCGCCTGCCTGTAGCGGCGTTGTTGCCACTCCGCCACCATGTT CGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCT CATCAACGAGGCCTGCTGCGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTC GTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTG CGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAATGCGC CGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGT ATTTGAAGCACCCAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGATTTAGA TGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCTTCTGG TGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATTTCCTG CGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGGAATGGAAACATTAAATT GTCACAGACAAGTAATGTCGATAAAAGANAGGAACCTGTTACCATAGAGATGAATGAACC AGTNTCACTAACTTTTGCACTGGAGTACCTGAACTTCTTTACAAAGCCACTCCACTCTCT TCA >OriGene 3' read for NM_002592 unedited
CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGTTTGAAATTCAAGT AACTTTATTTAAATTCAAAAACAATTCTTAAAACTGCATTTAGAGTCAAGACCCTTTTGT ATTATAAAAATCACAAGTATTTCTAAGAGACAAAAATACTTCTAGGTTAACTAGACCAGA TCTGACTTTGGACTTTATTCTTTAAACAAATTGCAGAGAATAGAGAAAAAAATAGGTTAT TTACAGAAAACAATATCTACATATGTACTTAGAGGTACAAATTTGGTGACAGAAAAGACT TCAGTATATGCTGGCATCTTAGAAGCAGTTCTCAAAGAGCTTAGTTTTATTTTCTTGAAT TTTAAGAATGCCTAAGATCCTTCTTCATCCTCGATCTTGGGAGCCAAGTAGTATTTTAAG TGTCCCATATCCGCAATTTTATACTCTACAACAAGGGGTACATCTGCAGACATACTGAGT GTCACCGTTGAAGAGAGTGGAGTGGCTTTTGTAAAGAAGTTCAGGTACCTCAGTGCAAAA GTTAGTTGAACTGGTTCATTCATCTCTATGGTAACAGCTTCCTCCTCTTTATCGACATTA CTTGTCTGTGACAATTTAATGTTTCCATTTCCAAGNTCTCCACTTGCAGAAATTTCACTC CGTCTTTTGCACAGGAAATTACCACAGCATCTCCAATATGCCTGAGATCTCGGCATATAC GTGCAAATTCACCACGAGGCATCTTTACTACACAGCTGTACCTCTGGTCTGAAATTCCAA GTTGTTAACATCTAAATCCATCCACTTCATTCATAGCCTGAAAACTTCTCTGGTTTGGGG CCTTAAAAACTACCGCCAAGTATCCCGGTATCTCCGGCCCTTAGGGATGAAACCTCATTG CCGGCCTTTTAAATTTTGGCTAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002592 |
| Insert Size | 1530 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002592.2, NP_002583.1 |
| RefSeq Size | 1355 bp |
| RefSeq ORF | 786 bp |
| Locus ID | 5111 |
| UniProt ID | P12004 |
| Domains | PCNA |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair |
| Gene Summary | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells
,Nakagawa, C;Senda-Murata, K;Kawakita-Yamaguchi, A;,
Mol Biol
[PCNA]
|
|
A Novel Technology for Multiplex Gene ExpressionAnalysis Directly from Whole Blood SamplesStabilized at Ambient Temperature Using anRNA-Stabilizing Buffer
,Buffer, RNAS;,
The Journal of Molecular Diagnostics
,PubMed ID 25684272
[PCNA]
|
|
The ADP-ribosyltransferase PARP10/ARTD10 interacts with Proliferating Cell Nuclear Antigen (PCNA) and is required for DNA damage tolerance
,Nicolae, CM;Aho, ER;Vlahos, AH;Choe, KN;De, S;Karras, GI;Moldovan, GL;,
J. Biol. Chem. April 2014
,PubMed ID 24695737
[PCNA]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201741 | PCNA (Myc-DDK-tagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1 |
CNY 2,400.00 |
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| RC201741L1 | Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
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| RC201741L2 | Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
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| RC201741L3 | Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201741L4 | Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG201741 | PCNA (tGFP-tagged) - Human proliferating cell nuclear antigen (PCNA), transcript variant 1 |
CNY 4,000.00 |
