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CD73 (NT5E) (NM_002526) Human Untagged Clone
CAT＃: SC118577
NT5E (untagged)-Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1
CNY 6,424.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118577 sequence for NM_002526 edited (data generated by NextGen Sequencing)
ATGTGTCCCCGAGCCGCGCGGGCGCCCGCGACGCTACTCCTCGCCCTGGGCGCGGTGCTG TGGCCTGCGGCTGGCGCCTGGGAGCTTACGATTTTGCACACCAACGACGTGCACAGCCGG CTGGAGCAGACCAGCGAGGACTCCAGCAAGTGCGTCAACGCCAGCCGCTGCATGGGTGGC GTGGCTCGGCTCTTCACCAAGGTTCAGCAGATCCGCCGCGCCGAACCCAACGTGCTGCTG CTGGACGCCGGCGACCAGTACCAGGGCACTATCTGGTTCACCGTGTACAAGGGCGCCGAG GTGGCGCACTTCATGAACGCCCTGCGCTACGATGCCATGGCACTGGGAAATCATGAATTT GATAATGGTGTGGAAGGACTGATCGAGCCACTCCTCAAAGAGGCCAAATTTCCAATTCTG AGTGCAAACATTAAAGCAAAGGGGCCACTAGCATCTCAAATATCAGGACTTTATTTGCCA TATAAAGTTCTTCCTGTTGGTGATGAAGTTGTGGGAATCGTTGGATACACTTCCAAAGAA ACCCCTTTTCTCTCAAATCCAGGGACAAATTTAGTGTTTGAAGATGAAATCACTGCATTA CAACCTGAAGTAGATAAGTTAAAAACTCTAAATGTGAACAAAATTATTGCACTGGGACAT TCGGGTTTTGAAATGGATAAACTCATCGCTCAGAAAGTGAGGGGTGTGGACGTCGTGGTG GGAGGACACTCCAACACATTTCTTTACACAGGCAATCCACCTTCCAAAGAGGTGCCTGCT GGGAAGTACCCATTCATAGTCACTTCTGATGATGGGCGGAAGGTTCCTGTAGTCCAGGCC TATGCTTTTGGCAAATACCTAGGCTATCTGAAGATCGAGTTTGATGAAAGAGGAAACGTC ATCTCTTCCCATGGAAATCCCATTCTTCTAAACAGCAGCATTCCTGAAGATCCAAGCATA AAAGCAGACATTAACAAATGGAGGATAAAATTGGATAATTATTCTACCCAGGAATTAGGG AAAACAATTGTCTATCTGGATGGCTCCTCTCAATCATGCCGCTTTAGAGAATGCAACATG GGCAACCTGATTTGTGATGCAATGATTAACAACAACCTGAGACACGCGGATGAAATGTTC TGGAACCACGTATCCATGTGCATTTTAAATGGAGGTGGTATCCGGTCGCCCATTGATGAA CGCAACAATGGCACAATTACCTGGGAGAACCTGGCTGCTGTATTGCCCTTTGGAGGCACA TTTGACCTAGTCCAGTTAAAAGGTTCCACCCTGAAGAAGGCCTTTGAGCATAGCGTGCAC CGCTACGGCCAGTCCACTGGAGAGTTCCTGCAGGTGGGCGGAATCCATGTGGTGTATGAT CTTTCCCGAAAACCTGGAGACAGAGTAGTCAAATTAGATGTTCTTTGCACCAAGTGTCGA GTGCCCAGTTATGACCCTCTCAAAATGGACGAGGTATATAAGGTGATCCTCCCAAACTTC CTGGCCAATGGTGGAGATGGGTTCCAGATGATAAAAGATGAATTATTAAGACATGACTCT GGTGACCAAGATATCAACGTGGTTTCTACATATATCTCCAAAATGAAAGTAATTTATCCA GCAGTTGAAGGTCGGATCAAGTTTTCCACAGGAAGTCACTGCCATGGAAGCTTTTCTTTA ATATTTCTTTCACTTTGGGCAGTGATCTTTGTTTTATACCAATAG Clone variation with respect to NM_002526.3 1126 a=>g >OriGene 5' read for NM_002526 unedited
NGGTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCGGCTCG CCCGCTTTCGCACCCAGTTCACGCGCCACAGCTATGTGTCCCCGAGCCGCGCGGGCGCCC GCGACGCTACTCCTCGCCCTGGGCGCGGTGCTGTGGCCTGCGGCTGGCGCCTGGGAGCTT ACGATTTTGCACACCAACGACGTGCACAGCCGGCTGGAGCAGACCAGCGAGGACTCCAGC AAGTGCGTCAACGCCAGCCGCTGCATGGGTGGCGTGGCTCGGCTCTTCACCAAGGTTCAG CAGATCCGCCGCGCCGAACCCAACGTGCTGCTGCTGGACGCCGGCGACCAGTACCAGGGC ACCTATCTGGTTCACCGTGTACAAGGGCGCCGAGGTGGCGCACTTCATGAACGCCCTGCG CTACGATGCCATGGCACTGGGAAATCATGAATTTGATAATGGTGTGGAAGGACTGATCGA GCCACTCCTCAAAGAGGCCAAATTTCCAATTCTGAGTGCAAACATTAAAGCAAAGGGGCC ACTAGCATCTCAAATATCAGGACTTTATTTGCCATATAAAGTTCTTCCTGTTGGTGATGA AGTTGTGGGAATCGTTGGATACACTTCCAAAGAAACCCCTTTTCTCTCAAATCCAGGGAC ANATTTAGTGTTTTGAAGATGAAATCACTGCATTACAACCTGAAGTAGATAAGTTAAAAA CTCTAAATGTGAACAAANATATTGCACTGGGACATTCCGGGTTTTTGAAATGGATAAACT CATCGCTCAAAAGTGAAGGGTGTNGGACCGTCCTGTTGGGAGGGACCTTCAACACATTTT CTTACACAGGCAATCCCACCTCCCAAGAAGTGCCTGCTGGGTAAGACCCCATTATTAGTC CTTTTG >OriGene 3' read for NM_002526 unedited
GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGCTTATCTTCAATTTTATTG TTGGGAATCTACAATTGCAAAGTGGTTCAAAGTCATTTCTTATACCACTTCACAATACAC ACACAGATTTAGGAAACTTAAAAAAAGTTCTTTAAAAAATTCTAATAGGATTTACCCTAT TTTACTGGCCAAGTGTAAAAAATAAACCCTAAATGTGCCTTTCTCTAATTTTCCTTGTAC AATCTTATTGACATTTTTTATAGCTAATCCATATATGCATAGAACCGAGGCTATTATTTT GCTGCAACAACTTTTATAAAAAGGTTATGAAGTTTAAAAGTATATTTTGTATCAAGAGTA CTTAATATTTGTAAACACTAAGAAACAAACATACTTCCTATCAAAACCTCTAGCTGCCAT TTGCACACTGTTTTTAAGGTGAAGCCTCCCATTTCTTGAATTACTTATGCAGAGCTGAGC AGGCTCATGTGGTTCCACTCCCACGTTAAGTATTTGTTCTTTGGGCATAGGTCATTTCAT CAGCATGGGTATTTTCTGACCACCCACTCAGAGGGTCCCACTTTCCAGCAGAAGTTCTCA TATCTGGGCTTAAGAATGATTTACAGTGACTGTTCAGATTTGGAGGCAGAGCGACTTAAA AAATGTATACCTCTTCCTAAAGTCATTTATTTTCTTCGAGAACTCTGGACATTCCATAAC TGGGTGTGTAGTATGAGTAGAATGAATTCAGTGCTAGCCTCTTGCTGGAGAGGACAAGTG CANNGTTAAATTACAGCTTATGTTAAAAGGNTCTCTTCTCATGATACCTTCATGTANAAN AAAGAGACAGAGGCANAGCTGATGGAATCTCATAAATAACAGCTAATGCCGTGTGTCACG CACTATAGCTAACAGTATCTGTTAAACAGTGTAATGCCCTTNACTCTTGCTTTCTATATT AAAAAGNCCTGGAGCCTGGTCTTCCCTTCTTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002526 |
| Insert Size | 3270 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002526.1, NP_002517.1 |
| RefSeq Size | 3547 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 4907 |
| UniProt ID | P21589 |
| Domains | Metallophos, 5_nucleotidaseC |
| Protein Families | ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ecto-5'-Nucleotidase and Thiopurine Cellular Circulation: Association with Cytotoxicity
,Fang Li, Brooke L. Fridley, Alice Matimba, Krishna R. Kalari, Linda Pelleymounter, Irene Moon, Yuan Ji, Gregory D. Jenkins, Anthony Batzler, Liewei Wang, and Richard M. Weinshilboum,
Drug Metab. Dispos., Dec 2010; 38: 2329 - 2338
[NT5E]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209568 | NT5E (Myc-DDK-tagged)-Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1 |
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|
| RC209568L1 | Lenti ORF clone of Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1, Myc-DDK-tagged |
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|
| RC209568L2 | Lenti ORF clone of Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1, mGFP tagged |
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|
| RC209568L3 | Lenti ORF clone of Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1, Myc-DDK-tagged |
CNY 8,808.00 |
|
| RC209568L4 | Lenti ORF clone of Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1, mGFP tagged |
CNY 8,808.00 |
|
| RG209568 | NT5E (tGFP-tagged) - Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1 |
CNY 8,008.00 |
