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MDM2 (NM_002392) Human Untagged Clone
CAT＃: SC118660
MDM2 (untagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2
CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACTFS; hdm2; HDMX; LSKB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002392 edited
AGGGCGTCGTGCTTCCGCGCGCCCCGTGAAGGAAACTGGGGAGTCTTGAGGGACCCCCGA CTCCAAGCGCGAAAACCCCGGATGGTGAGGAGCAGGCAAATGTGCAATACCAACATGTCT GTACCTACTGATGGTGCTGTAACCACCTCACAGATTCCAGCTTCGGAACAAGAGACCCTG GTTAGACCAAAGCCATTGCTTTTGAAGTTATTAAAGTCTGTTGGTGCACAAAAAGACACT TATACTATGAAAGAGGTTCTTTTTTATCTTGGCCAGTATATTATGACTAAACGATTATAT GATGAGAAGCAACAACATATTGTATATTGTTCAAATGATCTTCTAGGAGATTTGTTTGGC GTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAAATATATACCATGATCTACAGGAACTTG GTAGTAGTCAATCAGCAGGAATCATCGGACTCAGGTACATCTGTGAGTGAGAACAGGTGT CACCTTGAAGGTGGGAGTGATCAAAAGGACCTTGTACAAGAGCTTCAGGAAGAGAAACCT TCATCTTCACATTTGGTTTCTAGACCATCTACCTCATCTAGAAGGAGAGCAATTAGTGAG ACAGAAGAAAATTCAGATGAATTATCTGGTGAACGACAAAGAAAACGCCACAAATCTGAT AGTATTTCCCTTTCCTTTGATGAAAGCCTGGCTCTGTGTGTAATAAGGGAGATATGTTGT GAAAGAAGCAGTAGCAGTGAATCTACAGGGACGCCATCGAATCCGGATCTTGATGCTGGT GTAAGTGAACATTCAGGTGATTGGTTGGATCAGGATTCAGTTTCAGATCAGTTTAGTGTA GAATTTGAAGTTGAATCTCTCGACTCAGAAGATTATAGCCTTAGTGAAGAAGGACAAGAA CTCTCAGATGAAGATGATGAGGTATATCAAGTTACTGTGTATCAGGCAGGGGAGAGTGAT ACAGATTCATTTGAAGAAGATCCTGAAATTTCCTTAGCTGACTATTGGAAATGCACTTCA TGCAATGAAATGAATCCCCCCCTTCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAG AATTGGCTTCCTGAAGATAAAGGGAAAGATAAAGGGGAAATCTCTGAGAAAGCCAAACTG GAAAACTCAACACAAGCTGAAGAGGGCTTTGATGTTCCTGATTGTAAAAAAACTATAGTG AATGATTCCAGAGAGTCATGTGTTGAGGAAAATGATGATAAAATTACACAAGCTTCACAA TCACAAGAAAGTGAAGACTATTCTCAGCCATCAACTTCTAGTAGCATTATTTATAGCAGC CAAGAAGATGTGAAAGAGTTTGAAAGGGAAGAAACCCAAGACAAAGAAGAGAGTGTGGAA TCTAGTTTGCCCCTTAATGCCATTGAACCTTGTGTGATTTGTCAAGGTCGACCTAAAAAT GGTTGCATTGTCCATGGCAAAACAGGACATCTTATGGCCTGCTTTACATGTGCAAAGAAG CTAAAGAAAAGGAATAAGCCCTGCCCAGTATGTAGACAACCAATTCAAATGATTGTGCTA ACTTATTTCCCCTAGTTGACCTGTCTATAAGAGAATTATATATTTCTAACTATATAACCC TAGGAATTTAGACCACCTGAAATTTATTCACATATATCAAAGTGAGAAAATGCCT >OriGene 5' read for NM_002392 unedited
GCAACGCAATTCGCACGAGCCTCCCCGGATTAGTGCGTACGAGCGCCCAGTGCCCTGGCC CGGAGAGTGGAATGATCCCCGAGGCCCAGGGCGTCGTGCTTCCGCGCGCCCCGTGAAGGA AACTGGGGAGTCTTGAGGGACCCCCGACTCCAAGCGCGAAAACCCCGGATGGTGAGGATC AGGTAAATGTGCAATACCAACATGTCTGTACCTACTGATGGTGCTGTAACCACCTCACAG ATTCCAGCTTCGGAACAAGAGACCCTGGTTAGACCAAAGCCATTGCTTTTGAAGTTATTA AAGTCTGTTGGTGCACAAAAAGACACTTATACTATGAAAGAGGTTCTTTTTTATCTTGGC CAGTATATTATGACTAAACGATTATATGATGAGAAGCAACAACATATTGTATATTGTTCA AATGATCTTCTAGGAGATTTGTTTGGCGTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAA ATATATACCATGATCTACAGGAACTTGGTAGTAGTCAATCAGCAGGAATCATCGGACTCA GGTACATCTGTGAGTGAGAACAGGTGTCACCTTGAAAGTGGGAGTGATCAAAAGGACCTT GTACAAGAGCTTCANGAAGAGAAACCTTCATCTTCACATTTGGTTTCTAGACCATCTACC TCATCTAGAAAGGAGAGCCATTAGTGAGACAGAAGAAAATTCATATGAATTATCTGGTGA ACGACCAAANAAAGCGCCCCAAACCTGATAGTATTTCCCTTTCCTTTTGTGAAAAGCCTG CCTCTGTGTGGTAAAAAGGGAGATATGTTGGGAAAAGAAGCATTANCCGTGGAATCTACA GGGCCCCCATTCGAATCCGGATCTTGATGCCTGGGGTAAAGGAAA >OriGene 3' read for NM_002392 unedited
AAAANTTTGACTCTGGACCCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTAAAT AAGTTACATTTAAATGTCATATACACTATTTAAGAAAAAAAAAAACCAGTACTTCTCATT TAAGACAGAGTAGAAATTATTTAAAATTAGGAGTTGGTGTAAAGGATGAGCTACATATTC AAGTCAAATTATAGTAAGTATTCACTATTCCACTACCAAAGTAGGTCAATTATACTAAAG AGAAGAAATCTATGTGAATTGAGGCATTTTCTCACTTTGATATATGTGAATAAATTTCAG GTTGTCTAAATTCCTAGGGTTATATAGTTAGAAATATATAATTCTCTTATAGACAGGTCA ACTAGGGGAAATAAGTTAGCACAATCATTTGAATTGGTTGTCTACATACTGGGCAGGGCT TATTCCTTTTCTTTAGCTTCTTTGCACATGTAAAGCAGGCCATAAGATGTCCTGTTTTGC CATGGACAATGCAACCATTTTTAGGTCGACCTTGACAAATCACACAAGGTTCAATGGCAT TAAGGGGCAAACTAGATTCCACACTCTCTTCTTTGTCTTGGGTTTCTTCCCTTTCAAACT CTTTCACATCTTCTTGGCTGCTATAAATAATGCTACTAGAAGTTGATGGCTGAGAATAGT CTTCACTTTCTTGTGATTGTGAAGCTGNGTAATTTTATCATCATTTTCCTCACACATGAC TCTCTGGATCATCACTATAGGTTTTTTACATCACGAACTCAAAGCCTCTTCAGCTTGGGT GAGTTTCCAGTTGGCTTTTTAGAAATTCCCCTTATCTTTCCCTTTATCTCAGGAGCCCAT TTCACCAAGGCCCAACATTTTGCATTTGTGGAAAGGGGGGAATCTTCATTGCTGAAAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002392 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002392.2, NP_002383.2 |
| RefSeq Size | 2357 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 4193 |
| UniProt ID | Q00987 |
| Domains | zf-RanBP, MDM2 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Corosolic Acid Inhibits Cancer Progress Through Inactivating YAP in Hepatocellular Carcinoma
,null,
Oncology Research
,PubMed ID 32220262
[MDM2]
|
|
Nandrolone, an Anabolic Steroid, Stabilizes Numb Protein Through Inhibition of mdm2 in C2C12 Myoblasts
,Xin-Hua Liu, Shen Yao, Alice C. Levine, Alexander Kirschenbaum, Jiangping Pan, Yong Wu, Weiping Qin, Lauren Collier, William A. Bauman, and Christopher P. Cardozo,
J Androl, Nov 2012; 33: 1216 - 1223.
[MDM2]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219518 | MDM2 (Myc-DDK-tagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 |
CNY 5,488.00 |
|
| RC219518L1 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC219518L2 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged |
CNY 7,888.00 |
|
| RC219518L3 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC219518L4 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged |
CNY 7,888.00 |
|
| RG219518 | MDM2 (tGFP-tagged) - Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 |
CNY 7,088.00 |
