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JAK1 (NM_002227) Human Untagged Clone
CAT＃: SC118761
JAK1 (untagged)-Human Janus kinase 1 (JAK1)
CNY 11,936.00
| Cited in 2 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AIIDE; JAK1A; JAK1B; JTK3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002227 edited
ATGCAGTATCTAAATATAAAAGAGGACTGCAATGCCATGGCTTTCTGTGCTAAAATGAGG AGCTCCAAGAAGACTGAGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTC TATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGAGGAACTG TGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTCACAACCTCTTTGCCCTG TATGACGAGAACACCAAGCTCTGGTATGCTCCAAATCGCACCATCACCGTTGATGACAAG ATGTCCCTCCGGCTCCACTACCGGATGAGGTTCTATTTCACCAATTGGCATGGAACCAAC GACAATGAGCAGTCAGTGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAA AAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCTGTTTGCT CAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCCAAGACCGAGCAG GATGGACATGATATTGAGAACGAGTGTCTAGGGATGGCTGTCCTGGCCATCTCACACTAT GCCATGATGAAGAAGATGCAGTTGCCAGAACTGCCCAAGGACATCAGCTACAAGCGATAT ATTCCAGAAACATTGAATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATA AATAATGTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGACAGCAGC GTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGGAAACTTTGACAAAACAT TACGGTGCTGAAATATTTGAGACTTCCATGTTACTGATTTCATCAGAAAATGAGATGAAT TGGTTTCATTCGAATGACGGTGGAAACGTTCTCTACTACGAAGTGATGGTGACTGGGAAT CTTGGAATCCAGTGGAGGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAA CTGAAGCGGAAAAAACTGGAAAATAAACACAAGAAGGATGAGGAGAAAAACAAGATCCGG GAAGAGTGGAACAATTTTTCTTACTTCCCTGAAATCACTCACATTGTAATAAAGGAGTCT GTGGTCAGCATTAACAAGCAGGACAACAAGAAAATGGAACTGAAGCTCTCTTCCCACGAG GAGGCCTTGTCCTTTGTGTCCCTGGTAGATGGCTACTTCCGGCTCACAGCAGATGCCCAT CATTACCTCTGCACCGACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGT CATGGTCCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAGCGAGGAG GGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACATCCTCATGACCGTCACC TGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAGAAGCAGTTCAAGAACTTTCAGATC GAGGTGCAGAAGGGCCGCTACAGTCTGCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGA GACCTCATGAGCCACCTCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTA AAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTACTAAGAAA GCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTTTCGATCGGATCCTCAAG AAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGCACGAGAACACACATCTATTCTGGG ACCCTGATGGATTACAAGGATGACGAAGGAACTTCTGAAGAGAAGAAGATAAAAGTGATC CTCAAAGTCTTAGACCCCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGC ATGATGAGACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGTCCGCGAC GTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTCTGGATCTCTTCATGCAC CGGAAAAGCGATGTCCTTACCACACCATGGAAATTCAAAGTTGCCAAACAGCTGGCCAGT GCCCTGAGCTACTTGGAGGATAAAGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTC CTCCTGGCCCGTGAGGGCATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCC GGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATGGATTGCT CCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTGACAAGTGGAGCTTTGGA ACCACGCTCTGGGAAATCTGCTACAATGGCGAGATCCCCTTGAAAGACAAGACGCTGATT GAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCAGTGACACCATCATGTAAGGAGCTG GCTGACCTCATGACCCGCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCC ATCATGAGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCAGAAAAAAAA CCAGCAACTGAAGTGGACCCCACACATTTTGAAAAGCGCTTCCTAAAGAGGATCCGTGAC TTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTGCAGGTATGACCCCGAAGGGGACAAT ACAGGGGAGCAGGTGGCTGTTAAATCTCTGAAGCCTGAGAGTGGAGGTAACCACATAGCT GATCTGAAAAAGGAAATCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTAC AAAGGAATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATTTCTGCCT TCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAATAAACCTCAAACAGCAG CTAAAATATGCCGTTCAGATTTGTAAGGGGATGGACTATTTGGGTTCTCGGCAATACGTT CACCGGGACTTGGCAGCAAGAAATGTCCTTGTTGAGAGTGAACACCAAGTGAAAATTGGA GACTTCGGTTTAACCAAAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGAC CGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATTTTATATT GCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGCTGACTTACTGTGATTCA GATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATAGGCCCAACCCATGGCCAGATGACA GTCACAAGACTTGTGAATACGTTAAAAGAAGGAAAACGCCTGCCGTGCCCACCTAACTGT CCAGATGAGGTTTATCAACTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGACA AGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAA >OriGene 5' read for NM_002227 unedited
NAAGGGTTCAGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTGG CCCAGGCGCACACGGNAGTGCGCTTCTCTGAAGTAGCTTTGGAAAGTAGAGAAGAAAATC CAGTTTGCTTCTTGGAGAACACTGGACAGCTGAATAAATGCAGTATCTAAATATAAAAGA GGACTGCAATGCCATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAGGTGAA CCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTATCTGTCGGACAGGGAGCCCCT CCGGCTGGGCAGTGGAGAGTACACAGCAGAGGAACTGTGCATCAGGGCTGCACAGGCATG CCGTATCTCTCCTCTTTGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTG GTATGCTCCAAATCGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCG GATGAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTGTGGCG TCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAAAAGATTCCAGATGCAACCCC TCTCCTTGATGCCAGCTCACTGGAGTATCTGTTTGCTCAGGGACAGTATGATTTGGTGAA ATGCCTGGCTCCTATTCGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGA GTGTCTAGGGATGGCTGTCCTGGCCATCTCACACTATGCCATGATGAAAAAGATGCAGTT GCCAGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAACATTGAATAAGTCC ATCAGACAGAGGAACCTTCTCACCNAGATGCGGATAAATAATGGTTTTCAAGGATTTTCC TAAGGAATTTAACACC >OriGene 3' read for NM_002227 unedited
NNTTTTTTTTGGGTATTATCTATGNNACCGCGGCCGCATNCTANNGATCGATTTTTTTTT TTTTTTTTTTTTTTTTTTTAAAATCTTTAGTATATTTATTTGTATAAAGAGTAAACAAAG TGCATATAGAGTGGCCACAGGTTTGACACAGAGACCTTGGTGATGTAGGCTATGAACAAA TTTAAATGGCAACTTCATTGCTGCCACTGAACCAATCCTGAATTTGGGCTCAACAGGTGA AAAGTAACAATATCAAACGAATACTAAACAGCATAACAAAAAGATTTTCAGACTCTTGGT CATAAAGACCGTAATCGTTCACATTGAATCAATGACTAAACATTTTTGATTACCCAGCTA CCTCCAAGCAAACTGAAAACTGTCTAGTGGATCCTGAAGTCCATAGTGCCTCTAGCCGGG TCTTTCAAGTGTTGCACCACAGGGTGATGATTGATGGTAAAAACAAGGATCAACCCTTGT AGATCGGTGGTTAAGTATGAAACCCTCTAAGAACGTGCAGCGTATGTGGTATTCAAACTG GGTGCATACAGCATTCAAAACCAGTGCTGGAATAACTTGGCCAAAAATGGGTGAAGTTAT AAAAAGATATACCTTGCCCTTTCTTAAAAGCATGTGGTATAACCTTCAAGGTTCTGGGAA GGAATTCTTGATTAAAAGGATTACTGCTTAAGAAAAACACTGGGCCAAAGCTTTAGTTTT TTGGGAATTTAAATGCCCCAAAATTATCTATTTCCCAAACTTTTCCCCCCTGGGTAAAAC AAGCTTTTCTTAAAAAAGGGTGGCTAGTGGATCCCCCAAGGNACAGGAGCTAAACCACTG ANAGAACAAAGTTTAAGTCTACTGGTGAGAGATACAGTCATTTTTGTACAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002227 |
| Insert Size | 5100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002227.1, NP_002218.1 |
| RefSeq Size | 3541 bp |
| RefSeq ORF | 3429 bp |
| Locus ID | 3716 |
| UniProt ID | P23458 |
| Domains | pkinase, SH2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer |
| Gene Summary | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Dissociating STAT4 and STAT5 Signaling Inhibitory Functions of SOCS3: Effects on CD8 T Cell Responses
,Hwang, JY;Holland, JE;Valenteros, KB;Sun, Y;Usherwood, YK;Verissimo, AF;McLellan, JS;Grigoryan, G;Usherwood, EJ;,
Immunohorizons
,PubMed ID 31748225
[JAK1]
|
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Identification of somatic JAK1 mutations in patients with acute myeloid leukemia
,Zhifu Xiang, Yu Zhao, Vesselin Mitaksov, Daved H Fremont, Yumi Kasai, AnnaLynn Molitoris, Rhonda E Ries, Tracie L Miner, Michael D McLellan, John F DiPersio, Daniel C Link, Jacqueline E Payton, Timothy A Graubert, Mark Watson, William Shannon, Sharon E Heath, Rakesh Naggarajan, Elaine R Mardis, Richard K Wilson, Timothy J Ley, and Michael H Tomasson,
Blood, Dec 2007; 10.1182/blood-2007-05-090308.
[JAK1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213878 | JAK1 (Myc-DDK-tagged)-Human Janus kinase 1 (JAK1) |
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| RC213878L1 | Lenti ORF clone of Human Janus kinase 1 (JAK1), Myc-DDK-tagged |
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| RC213878L2 | Lenti ORF clone of Human Janus kinase 1 (JAK1), mGFP tagged |
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| RC213878L3 | Lenti ORF clone of Human Janus kinase 1 (JAK1), Myc-DDK-tagged |
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| RC213878L4 | Lenti ORF clone of Human Janus kinase 1 (JAK1), mGFP tagged |
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| RG213878 | JAK1 (tGFP-tagged) - Human Janus kinase 1 (JAK1) |
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| SC318528 | JAK1 (untagged)-Human Janus kinase 1 (JAK1) |
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