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Aspartate Aminotransferase (GOT1) (NM_002079) Human Untagged Clone
CAT＃: SC118816
GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AST1; ASTQTL1; cAspAT; cCAT; GIG18 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118816 sequence for NM_002079 edited (data generated by NextGen Sequencing)
ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAG CTCACTGCCGACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCA TATCGCACGGATGACTGCCATCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAG ATTGCTAATGACAATAGCCTAAATCACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTC CGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGG GTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTA GCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGC TACTGGGATGCAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAAT GCTCCTGAGTTCTCCATTGTTGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGAC CCAACTCCGGAGCAGTGGAAGCAGATTGCTTCTGTCATGAAGCACCGGTTTCTGTTCCCC TTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACCTGGAGAGAGATGCCTGGGCC ATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTTCTCCAAGAAC TTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCC GCCCAGGGAGCACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGG ACAGGTAATGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCA CGACTAGAAGCCCTCAAAACCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATG TTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATCTGGTCAATGAAAAGCACATC TACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAATCTAGATTAC GTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAGTGA Clone variation with respect to NM_002079.2 >OriGene 5' read for NM_002079 unedited
ACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCCAGACCTGACCTGCCAGCTCCGGG CGTGGGGTGAAATCTCTTGATTCCTAGTCTCTCGATATGGCACCTCCGTCAGTCTTTGCC GAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAGCTCACTGCCGACTTCAGGGAGGAT CCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCATATCGCACGGATGACTGCCATCCC TGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAGATTGCTAATGACAATAGCCTAAAT CACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTCCGGAGCTGTGCTTCTCGTCTTGCC CTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGGGTAGGAGGTGTGCAATCTTTGGGG GGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTAGCGCGTTGGTACAATGGAACAAAC AACAAGAACACACCTGTCTATGTGTCCTCACCAACCTGNGAGAATCACAATGCTGTGTTT TCCGCTGCTGGTTNTAAAGACATTCGGTCCTATCGCTACTGNGATGCAGAGAGAGAGGAT TGGACCTCCAGGGCTTCCTGAATGATCTGGAGAATGCTCCTGAGTTCTCCATTGNTGTCC TCCACGCCTGTGCACACCACCCAACTGGNGATGACCNCACTCNGNAGCAGTGGAAGCAGA TTGCTTCTGTCATGAAGCACCGTTTNNCTGTNCCCTTCTTTGACTCANCTATCAGGNCTT CGCATCTGGAAACCCTGAGAGAGATGCCCTGGCCATTCGCTATTTTGTGTCTGAAGGCTT CAGTTCTTCTGTGCCCAGTCCTCTNCAGAACTNCNGGCTCTACATGANAGAGTTCGGAAT CTGACTGTGGGTGGAAAAGACCTGANAGCTNCTTGCAGGTCCTTCCCAATGGGAAAGATC GTGCGATTACTTGTTCCATNCCCCGCCAGGAGACAGATTGGGGCCGACCCTCCTAACCTG ACCTTTGGGAAGGG >OriGene 3' read for NM_002079 unedited
AAACCCCCGGCCCNCCCTAACTCCTATATCCCCTGCTTTTACTTGANCCGCGGCACGCTA TTCTANATCGAGTTTTTTTTTTTTTTTTTTGCACAACATTTTTATTTTATTTTTTTAGGC GAAGTACAACACAATACAATGGCTCAAAAATATCAGAATGCACTACGCACATCACGAGTA AATACTGTCTGGTAAAACTTGTTTCAGTTAAATATGTACGTGCCCGCGCATGTCATGATT AAATATCCTTCTTACCACAGTCACCCTAAAGAACCAAAGCTTAGGACTAGGGACACACCC ATGCAGAAAGAGCAGGGAGACCAGACACTCTGGGTTGAGATGATGAATTTAATGCCGCAG CCGACACCCACATTCACACTTTGGCTCCTTTACACAACTTGATCTTTGGGACTACCAATT TGTACAGAGTTAAACACCACATAGAAGCACGCGGCCGCCACACACAACACTCCTTTTTAG TGAGAGGGACACCACCCCTATGTCTCATGATCCAAGCACTCTTTTATTCTTAAATAAAAA TCTTAATTTGCCTTTGCACCTCAAAGGCTCTAATCCCAGTCTTCAAATTCGTCTCAAGGG ATGTTCTCTTCATGTGGGGCTCGGTCCAAACCGCACGCTGCCTTACCACACCCGCCTTTC ACCTCCTCGCAGCGTCCCTCTACCCCCATGGCCATGTCCATGCACGTTTCGTCCCGCCAG CGACCCCCCCTTAACAGAAACTTCCTTTGGGGCCACTGGACCGGGTGGAGTTCCCTTCCC CTGCATTCTCGGTCACCTGTCTTTATGGCCTGGAGAGTGCCACCCCCACACTCCAATCTT TCCGGGCCAAACCCACTTTACGCCCGATTTCCACACCTCGCCAACCCCTTCCAATTCCTC TTTTACTTGGCTCAAAACTTACAACCGCTTCGGGGTTCACACCAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002079 |
| Insert Size | 2080 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002079.1, NP_002070.1 |
| RefSeq Size | 1941 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 2805 |
| UniProt ID | P17174 |
| Domains | aminotran_1_2 |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism |
| Gene Summary | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201757 | GOT1 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 3,656.00 |
|
| RC201757L1 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201757L2 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), mGFP tagged |
CNY 5,890.00 |
|
| RC201757L3 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201757L4 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), mGFP tagged |
CNY 5,890.00 |
|
| RG201757 | GOT1 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 5,256.00 |
|
| SC320596 | GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 3,656.00 |
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