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Aspartate Aminotransferase (GOT1) (NM_002079) Human Untagged Clone
CAT＃: SC320596
GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AST1; ASTQTL1; cAspAT; cCAT; GIG18 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_002079.1
GAAATCTCTTGATTCCTAGTCTCTCGATATGGCACCTCCGTCAGTCTTTGCCGAGGTTCC
GCAGGCCCAGCCTGTCCTGGTCTTCAAGCTCACTGCCGACTTCAGGGAGGATCCGGACCC CCGCAAGGTCAACCTGGGAGTGGGAGCATATCGCACGGATGACTGCCATCCCTGGGTTTT GCCAGTAGTGAAGAAAGTGGAGCAGAAGATTGCTAATGACAATAGCCTAAATCACGAGTA TCTGCCAATCCTGGGCCTGGCTGAGTTCCGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGA TGACAGCCCAGCACTCAAGGAGAAGCGGGTAGGAGGTGTGCAATCTTTGGGGGGAACAGG TGCACTTCGAATTGGAGCTGATTTCTTAGCGCGTTGGTACAATGGAACAAACAACAAGAA CACACCTGTCTATGTGTCCTCACCAACCTGGGAGAATCACAATGCTGTGTTTTCCGCTGC TGGTTTTAAAGACATTCGGTCCTATCGCTACTGGGATGCAGAGAAGAGAGGATTGGACCT CCAGGGCTTCCTGAATGATCTGGAGAATGCTCCTGAGTTCTCCATTGTTGTCCTCCACGC CTGTGCACACAACCCAACTGGGATTGACCCAACTCCGGAGCAGTGGAAGCAGATTGCTTC TGTCATGAAGCACCGGTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTCGCATC TGGAAACCTGGAGAGAGATGCCTGGGCCATTCGCTATTTTGTGTCTGAAGGCTTCGAGTT CTTCTGTGCCCAGTCCTTCTCCAAGAACTTCGGGCTCTACAATGAGAGAGTCGGGAATCT GACTGTGGTTGGAAAAGAACCTGAGAGCATCCTGCAAGTCCTTTCCCAGATGGAGAAGAT CGTGCGGATTACTTGGTCCAATCCCCCCGCCCAGGGAGCACGAATTGTGGCCAGCACCCT CTCTAACCCTGAGCTCTTTGAGGAATGGACAGGTAATGTGAAGACAATGGCTGACCGGAT TCTGACCATGAGATCTGAACTCAGGGCACGACTAGAAGCCCTCAAAACCCCTGGGACCTG GAACCACATCACTGATCAAATTGGCATGTTCAGCTTCACTGGGTTGAACCCCAAGCAGGT TGAGTATCTGGTCAATGAAAAGCACATCTACCTGCTGCCAAGTGGTCGAATCAACGTGAG TGGCTTAACCACCAAAAATCTAGATTACGTGGCCACCTCCATCCATGAAGCAGTCACCAA AATCCAGTGAAGAAACACCACCCGTCCAGTACCACCAAAGTAGTTCTCTGTCATGTGTGT TCCCTGCCTGCACAAACCTACATGTACATACCATGGATTAGAGACACTTGCAGGACTGAA AGGCTGCTCTGGTGAGGCAGCCTCTGTTTAAACCGGCCCCACATGAAGAGAACATCCCTT GAGACGAATTTGGAGACTGGGATTAGAGCCTTTGGAGGTCAAAGCAAATTAAGATTTTTA TTTAAGAATAAAAGAGTACTTTGATCATGAGAAAAAAAACAAAAAAAAAAAAAAAAAAAA AAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_002079 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002079.1, NP_002070.1 |
| RefSeq Size | 1941 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 2805 |
| UniProt ID | P17174 |
| Domains | aminotran_1_2 |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism |
| Gene Summary | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibition of pyruvate carboxylase by 1α,25-dihydroxyvitamin D promotes oxidative stress in early breast cancer progression
,Wilmanski, T;Zhou, X;Zheng, W;Shinde, A;Donkin, SS;Wendt, M;Burgess, JR;Teegarden, D;,
Cancer Lett.
,PubMed ID 29024812
[GOT1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201757 | GOT1 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 3,656.00 |
|
| RC201757L1 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201757L2 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), mGFP tagged |
CNY 5,890.00 |
|
| RC201757L3 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201757L4 | Lenti ORF clone of Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), mGFP tagged |
CNY 5,890.00 |
|
| RG201757 | GOT1 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 5,256.00 |
|
| SC118816 | GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) |
CNY 3,656.00 |
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