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GFAP (NM_002055) Human Untagged Clone
CAT＃: SC118873
GFAP (untagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 1
CNY 3,656.00
| Cited in 7 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALXDRD |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118873 sequence for NM_002055 edited (data generated by NextGen Sequencing)
ATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCCTCAGGGGAGATG ATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGCCTCTCCCTGGCT CGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGGGCACTCAATGCT GGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTCAATGACCGCTTT GCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCGCTGGCTGCTGAG CTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTACCAGGCTGAGCTG CGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGGCTGGAGGTTGAG AGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAGGATGAAACCAAC CTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCAGATGAAGCCACC CTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAGATCCGGTTCTTG AGGAAGATCCACGAGGAGGAGGTTCGGGAACTCCAGGAGCAGCTGGCCCGACAGCAGGTC CATGTGGAGCTTGACGTGGCCAAGCCAGACCTCACCGCAGCCCTGAAAGAGATCCGCACG CAGTATGAGGCAATGGCGTCCAGCAACATGCATGAAGCCGAAGAGTGGTACCGCTCCAAG TTTGCAGACCTGACAGACGCTGCTGCCCGCAACGCGGAGCTGCTCCGCCAGGCCAAGCAC GAAGCCAACGACTACCGGCGCCAGTTGCAGTCCTTGACCTGCGACCTGGAGTCTCTGCGC GGCACGAACGAGTCCCTGGAGAGGCAGATGCGCGAGCAGGAGGAGCGGCACGTGCGGGAG GCGGCCAGTTATCAGGAGGCGCTGGCGCGGCTGGAGGAAGAGGGGCAGAGCCTCAAGGAC GAGATGGCCCGCCACTTGCAGGAGTACCAGGACCTGCTCAATGTCAAGCTGGCCCTGGAC ATCGAGATCGCCACCTACAGGAAGCTGCTAGAGGGCGAGGAGAACCGGATCACCATTCCC GTGCAGACCTTCTCCAACCTGCAGATTCGAGAAACCAGCCTGGACACCAAGTCTGTGTCA GAAGGCCACCTCAAGAGGAACATCGTGGTGAAGACCGTGGAGATGCGGGATGGAGAGGTC ATTAAGGAGTCCAAGCAGGAGCACAAGGATGTGATGTGA Clone variation with respect to NM_002055.4 141 g=>a >OriGene 5' read for NM_002055 unedited
GCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTC ATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGG CACGAGGGCAGGATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCC TCAGGGGAGATGATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGC CTCTCCCTGGCTCGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGG GCACTCAATGCTGGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTC AATGACCGCTTTGCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCG CTGGCTGCTGAGCTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTAC CAGGCTGAGCTGCGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGG CTGGAGGTTGAGAGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAG GATGAAACCAACCTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCA GATGAAGCCACCCTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAG ATCCGGTTCTTGAGGAAGATCCACGANGAGGGAGGTCGGNAACTNCAGNAGCAGCTGGCC CGACAGCNAGTCCATGTGGAGCTTGACGTGGCCAGCCAGACCTCACGCAGCCCTGAAAGA GATCGCACGCAGTATGAGGGCATGGCGTCCAGCACATGCATGAAGCCCGAGAGTGTACCG CTCCCAGTTGCAGACCTGACGACGCTGCTGNCCGCACGCGNAGCTGNTCCGCCAGGCCAG CCACGAGCCACGACTACCGNGCCAGNTGCAGTCCTTGACTGCGACCTGNNATCTCTGCNC GGNACAN >OriGene 3' read for NM_002055 unedited
TTGGTCGCTGCCGCAATTTANATCGAGTTTTTTTTTTTTTTTTTTGAGGGCGCAGCATTT GTCTTTATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAGCAGCAGCA GCAGCAGCAGGTGGCAGGAAGGAGTGGAGGGAAAGGACACCAAGTCTTGGGGAGAGGGAA GGACCCTTCTTCGGCCTTAGAGGGGAGAGGAGAACCCTGAAGTGGGCCCTCCCAGTCCCA TCTCTGGGCACAGATCCCACCAGTCTGCTCACCAGTCTGCTCAGTCAAAGCAGAGTGGGT GCACTGGGGTGGACGTGTCAGCCCTGAGCACCCGGCCTCCAGGCTGCAGGAATATGAGCC AGTGTCTTCACTTTGCTCGTGCCTCAGTTTTACAATTGTAAAATAGGGCACTACCTAGAA TACTGGGTACATTTTGTGTGTGAGTAAGAAGGGACCGCAAGAGGCCCTTGGCTTAGGGAA AAGCAGCCGGTCACTATGGGGTGGAGAGGGTCAGACGTGTGGCAGTGGCTTACTGGGGTT AAGAAGCAGCAGTCCCAGGGGCTCAGAGGCTGGGGCGGCCTGGTATGACACAGCAAGGAA GAGGCCTTTAGAAATGGGACAAAGTCATGCCCTGCCCCCATGGATACATCCCCTTTCTCT CCTGTTTCAGCATCTTCAAGAGGATGAGTCACTTCCTTAATTCCCACAATCCAGAGGCCA AGTGCAACTGGTCACCCACAACCCCTACTTGTATGCCTAGCGCCATCCCAATTGCCTCCT CCTCCATCTCTACCAGCGTCCCCTCCAGTCTGCACGGGGCAGTCCTCCTGGGCTTGACCT CTCTGTACCCACAGCTGGGGGCCAGGCAGCCCCCCTCTATCCCTCCCAGCACTNATCCCT CTNCACGTACACCACAGATCCCCCCAGTGCTGAGAAATCAAGCTCCACCTGCCCACAGCG TGCCCACAGATGGCATC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002055 |
| Insert Size | 3075 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002055.2, NP_002046.1 |
| RefSeq Size | 3035 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 2670 |
| UniProt ID | P14136 |
| Domains | filament, filament_head |
| Protein Families | ES Cell Differentiation/IPS |
| Gene Summary | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes a predominant isoform (1, also known as alpha). |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Adult-Onset Alexander Disease: New Causal Sequence Variant in the GFAP Gene
,null,
Neurology: Genetics
,PubMed ID 35620133
[GFAP]
|
|
Site-specific phosphorylation and caspase cleavage of GFAP are new markers of Alexander Disease severity
,Battaglia, RA;Beltran, AS;Delic, S;Dumitru, R;Robinson, JA;Kabiraj, P;Herring, LE;Madden, VJ;Ravinder, N;Willems, E;Newman, RA;Quinlan, RA;Goldman, JE;Perng, MD;Inagaki, M;Snider, NT;,
Elife
,PubMed ID 31682229
[GFAP]
|
|
A Novel Mutation of GFAP Causing Adult-Onset Alexander Disease
,Ciammola, A;Sangalli, D;Sassone, J;Poletti, B;Carelli, L;Banfi, P;Pappacoda, G;Ceccherini, I;Grossi, A;Maderna, L;Pingue, M;Girotti, F;Silani, V;,
Front Neurol
,PubMed ID 31781017
[GFAP]
|
|
Clinical and immunological characteristics of the spectrum of GFAP autoimmunity: a case series of 22 patients.
,null,
Journal of neurology, neurosurgery, and psychiatry
,PubMed ID 28951498
[GFAP]
|
|
The cysteine residue of glial fibrillary acidic protein is a critical target for lipoxidation and required for efficient network organization
,Viedma-Poyatos, Á;de Pablo, Y;Pekny, M;Pérez-Sala, D;,
Free Radic. Biol. Med.
,PubMed ID 29635011
[GFAP]
|
|
Human Traumatic Brain Injury Induces Autoantibody Response against Glial Fibrillary Acidic Protein and Its Breakdown Products
,Zhang, Z;Zoltewicz, JS;Mondello, S;Newsom, KJ;Yang, Z;Yang, B;Kobeissy, F;Guingab, J;Glushakova, O;Robicsek, S;Heaton, S;Buki, A;Hannay, J;Gold, MS;Rubenstein, R;Lu, XC;Dave, JR;Schmid, K;Tortella, F;Robertson, CS;Wang, KK;,
PLoS ONE
,PubMed ID 24667434
[GFAP]
|
|
Characterization of Antibodies that Detect Human GFAP after Traumatic Brain Injury
,null,
Biomarker Insights
,PubMed ID 22798722
[GFAP]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204548 | GFAP (Myc-DDK-tagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 1 |
CNY 3,656.00 |
|
| RC204548L1 | Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204548L2 | Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204548L3 | Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204548L4 | Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG204548 | GFAP (tGFP-tagged) - Human glial fibrillary acidic protein (GFAP), transcript variant 1 |
CNY 5,256.00 |
