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CD47 (NM_001777) Human Untagged Clone
CAT＃: SC119049
CD47 (untagged)-Human CD47 molecule (CD47), transcript variant 1
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IAP; MER6; OA3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001777 edited
GAATTCGGCACGAGGCGCGTGCGCGCGGCCGTGCAGCCTGGGCAGTGGGTCCTGCCTGTG ACGCGCGGCGGCGGTCGGTCCTGCCTGTAACGGCGGCGGCGGCTGCTGCTCCAGACACCT GCGGCGGCGGCGGCGACCCCGCGGCGGGCGCGGAGATGTGGCCCCTGGTAGCGGCGCTGT TGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATAAAACAAAATCTGTAG AATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATGGAGGCAC AAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTACACCTTTG ATGGAGCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATTGAAGTCT CACAATTACTAAAAGGAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTCTCACACA CAGGAAACTACACTTGTGAAGTAACAGAATTAACCAGAGAAGGTGAAACGATCATCGAGC TAAAATATCGTGTTGTTTCATGGTTTTCTCCAAATGAAAATATTCTTATTGTTATTTTCC CAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAACACTTAAATATAGAT CCGGTGGTATGGATGAGAAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCACTGTCA TTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGTGAATATTCATTAAAGAATGCTACTG GCCTTGGTTTAATTGTGACTTCTACAGGGATATTAATATTACTTCACTACTATGTGTTTA GTACAGCGATTGGATTAACCTCCTTCGTCATTGCCATATTGGTTATTCAGGTGATAGCCT ATATCCTCGCTGTGGTTGGACTGAGTCTCTGTATTGCGGCGTGTATACCAATGCATGGCC CTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTAGCACAATTACTTGGACTAGTTTATA TGAAATTTGTGGCTTCCAATCAGAAGACTATACAACCTCCTAGGAAAGCTGTAGAGGAAC CCCTTAATGCATTCAAAGAATCAAAAGGAATGATGAATGATGAATAACTGAAGTGAAGTG ATGGACTCCGATTTGGAGAGTAGTAAGACGTGAAAGGAATACACTTGTGTTTAAGCACCA TGGCCTTGATGATTCACTGTTGGGGAGAAGAAACAAGAAAAGTAACTGGTTGTCACCTAT GAGACCCTTACGTGATTGTTAGTTAAGTTTTTATTCAAAGCAGCTGTAATTTAGTTAATA AAATAATTATGATCTATGTTGTTTGCCCAATTGAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_001777 unedited
GGCAACACCATTCGGCACGAGGCGCGTGCGCTCGGCCGTGCAGCCTGGGCAGTGGGTCCT GCCTGTGACGCGCGGCGGCGGTCGGTCCTGCCTGTAACGGCGGCGGCGGCTGCTGCTCCA GACACCTGCGGCGGCGGCGGCGACCCCGCGGCGGGCGCGGAGATGTGGCCCCTGGTAGCT GCGTTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATAAAACAAAA TCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATG GAGGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTAC ACCTTTGATGGAGCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATT GAAGTCTCACAATTACTAAAAGGAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTC TCACACACAGGAAACTACACTTGTGAAGTAACAGAATTAACCAGAGAAGGTGAAACGATC ATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCANATGAAAATATTCTTATTGTT ATTTTCCCCAATTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAACACTTAAA TATAGATCCGGTGGTATGGATGAGAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCA CTGTCCATGGCATTGTTGGAAGCCATTCTTTTCGTCCCAGGTGAATATTCTTAAAGAATG CTACTGGCCCTGGTTTAATGTGACTTCTACAGGAATATTAATATTACCTTCCTACTTGTG TTTAGACAACGATTGAATTACCCCCCTTCGTCATGCCACATTGGGTATTCAGGGGAAAGC CT >OriGene 3' read for NM_001777 unedited
GGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCAATTGGGCAAACAACATAGA TCATAATTATTTTATTAACTAAATTACAGCTGCTTTGAATAAAAACTTAACTAACAATCA CGTAAGGGTCTCATAGGTGACAACCAGTTACTTTTCTTGTTTCTTCTCCCCAACAGTGAA TCATCAAGGCCATGGTGCTTAAACACAAGTGTATTCCTTTCACGTCTTACTACTCTCCAA ATCGGAGTCCATCACTTCACTTCAGTTATTCATCATTCATCATTCCTTTTGATTCTTTGA ATGCATTAAGGGGTTCCTCTACAGCTTTCCTAGGAGGTTGTATAGTCTTCTGATTGGAAG CCACAAATTTCATATAAACTAGTCCAAGTAATTGTGCTAGAGCTAAGATACTCAAACCTG AAATCAGAAGAGGGCCATGCATTGGTATACACGCCGCAATACAGAGACTCAGTCCAACCA CAGCGAGGATATAGGCTATCACCTGAATAACCAATATGGCAATGACGAAGGAGGTTAATC CAATCGCTGTACTAAACACATAGTAGTGAAGTAATATTAATATCCCTGTAGAAGTCACAA TTAAACCAAGGCCAGTAGCATTCTTTAATGAATATTCACCTGGGACGAAAAGAATGGCTC CAACAATGACAATGACAGTGATCACTAGTCCAGCAACAAGTAAAGCAATTGTTTTCTCAT CCATACCACCGGATCTATATTTAAGTGTTTTAATACCAAACTGTCCCCAAACAGGAGTAT AGCANAAATTGGGAAAATACACTAAGATATTTTCATTTGGAGAATACCATGAAACACACG ATATTTAGCTCGATGATCGTTTCACCTTCTCTGGNTAAATTCTGTACTTCACAAGTGTAG TTNCCCTGGTGTGTGAGACAGCATCACTCTTATNCCATCTCAAGAGGCATCTCCTTCTAG TAATNGNGAGACTTCATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001777 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001777.3, NP_001768.1 |
| RefSeq Size | 5346 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 961 |
| UniProt ID | Q08722 |
| Domains | CD47 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | ECM-receptor interaction |
| Gene Summary | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218813 | CD47 (Myc-DDK-tagged)-Human CD47 molecule (CD47), transcript variant 1 |
CNY 2,400.00 |
|
| RC218813L1 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218813L2 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RC218813L3 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC218813L4 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG218813 | CD47 (tGFP-tagged) - Human CD47 molecule (CD47), transcript variant 1 |
CNY 4,000.00 |
