ATP6V1B1 (NM_001692) Human Untagged Clone
CAT#: SC119114
ATP6V1B1 (untagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1)
CNY 4,856.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP6B1; DRTA2; RTA1B; VATB; VMA2; VPP3 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001692, the custom clone sequence may differ by one or more nucleotides
ATGGCCATGGAGATAGACAGCAGGCCTGGGGGGCTCCCCGGCAGTAGCTGCAACCTAGGTGCAGCCCGAG AACACATGCAGGCGGTCACCCGAAACTACATCACCCACCCCCGTGTCACCTACAGGACTGTGTGCAGCGT GAACGGGCCCCTGGTGGTGCTGGACCGGGTCAAGTTTGCCCAGTATGCGGAGATCGTCCACTTCACCCTC CCAGATGGGACTCAGAGGAGCGGGCAGGTGCTTGAGGTGGCTGGCACCAAGGCGATTGTTCAGGTGTTTG AAGGGACATCAGGGATCGATGCCAGGAAGACCACTTGCGAATTTACAGGGGACATCCTACGAACTCCGGT GTCAGAGGACATGCTGGGTCGGGTTTTCAATGGCTCCGGCAAGCCCATTGACAAGGGGCCAGTGGTCATG GCGGAGGACTTTCTGGATATCAATGGCCAGCCCATCAACCCGCACTCCCGCATCTACCCCGAGGAGATGA TTCAGACGGGCATTTCTCCTATTGACGTCATGAACAGCATTGCCCGCGGCCAGAAGATCCCCATCTTCTC AGCAGCCGGGCTCCCCCACAATGAGATTGCCGCTCAGATCTGCCGCCAGGCGGGGCTGGTGAAGAAGTCC AAGGCTGTGCTGGATTACCATGACGACAACTTCGCCATCGTCTTTGCAGCCATGGGGGTGAACATGGAGA CAGCCAGATTCTTCAAGTCTGACTTTGAGCAGAATGGAACCATGGGGAACGTCTGCCTCTTCCTGAACTT GGCCAATGACCCCACGATCGAGCGGATCATCACCCCGCGCCTGGCGCTGACCACTGCTGAATTCCTTGCC TACCAGTGTGAGAAGCATGTGCTGGTCATACTGACGGACATGAGTTCCTATGCAGAGGCCTTGCGGGAGG TCTCTGCTGCTAGAGAGGAGGTGCCTGGGCGCCGAGGGTTTCCTGGATATATGTACACAGACCTGGCCAC CATCTACGAGCGGGCGGGCCGCGTGGAGGGTCGGGGAGGATCCATCACACAGATCCCCATCCTCACCATG CCCAACGACGATATCACCCACCCTATCCCAGACTTGACGGGCTTCATCACAGAGGGACAGATCTACGTGG ACAGACAGCTTCACAACAGACAGATCTACCCCCCCATCAACGTGCTCCCTTCCCTGTCGCGGCTGATGAA GTCAGCCATTGGGGAAGGCATGACAAGAAAGGACCATGGAGATGTCTCCAACCAGCTGTACGCCTGCTAT GCCATCGGGAAGGACGTGCAGGCCATGAAGGCAGTAGTTGGGGAGGAGGCGCTCACCTCTGAGGACCTGC TCTACCTGGAATTCCTGCAGAAGTTTGAGAAGAACTTCATCAATCAGGGCCCCTACGAGAACCGCTCGGT GTTCGAGTCGCTGGACCTGGGCTGGAAGCTGCTGCGCATCTTCCCCAAGGAGATGCTGAAGCGCATTCCG CAGGCCGTGATCGACGAGTTCTATTCCCGCGAGGGGGCGCTGCAGGACCTCGCGCCTGACACTGCGCTCT AG >OriGene 5' read for NM_001692 unedited
GGGTTCAAATTTTGTNATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGCTCAG ACACTGGNGCTCCCAGCTGGGGACTGCTCCACGGCCATGGAGATAGACAGCAGGCCTGGG GGGCTCCCCGGCAGTAGCTGCAACCTAGGTGCAGCCCGAGAACACATGCAGGCGGTCANC CCGAAACTACATCACCCACCCCCGTGTCACCTACAGGACTGTGTGCAGCGTGAACGGGCC CCTGGTGGTGCTGGACCGGGTCAAGTTTGCCCAGTATGCGGAGATCGTCCACTTCACCCT CCCAGATGGGACTCAGAGGAGCGGGCAGGTGCTTGAGGTGGCTGGCACCAAGGCGATTGT TCAGGTGTTTGAAGGGACATCAGGGATCGATGCCAGGAAGACCACTTGCGAATTTACAGG GGACATCCTACGAACTCCGGTGTCAGAGGACATGCTGGGTCGGGTTTTCAATGGCTCCGG CAAGCCCATTGACAAGGGGCCAGTGGTCATGGCGGAGGACTTTCTGGATATCAATGGCCA GCCCATCAACCCGCACTCCCGCATCTACCCCGAGGAGATGATTCAGACGGGCATTTCTCC TATTGACGTCATGAACAGCATTGCCCGCGGCCAGAAGATCCCCATCTTCTCAGCAGCCGG GCTTCCCCACAATGAGATTGCCGCTCAGATCTGCCGCCAGGCGGGGCTGGTGAAGAAGTC CAAGGCTGTGCTGGATTACCATGACGANCACTTCGCCATCGTCTTTGCAGCCATGGGGGT GAACATGGAGACAGCCAGATTCTTCAGTCTGACNTTGAGCAGAATGGAACCATGGGAACG TCTGCCTCTTCCTGAACTTGGCAATGACCCCCGATCGAGCGATCATCACCCGCGCCTGGG CTGACACTGCTGAATTNCTTGCTCN >OriGene 3' read for NM_001692 unedited
AGTTTTATATATATCTTTTTTTTTTTCAGTTACTTTTTATTTTTATTTTGTGGGCTTTTA ACATAAAATACAGATGCTCCCGTCAGAGTAAGGCATCGCAACCTTCAGTTCTTCCGCAGC ACCGGGAGTCGAGGGGGAGGCATGGAGCGCGGGAAAAGGAATCGAGCGAGGGAAAGGGAT GGAGCGTGCGGCGCCCCCACCACCTCGGAGCCAGCGGAGGGCGGAGGGCGGCGCATAAGC CGCTGGTTGGGGTGGCGAGGGAGGGGAGACCCGGGAGCCGAGGGTAGGTTCCCTGCCGGT GTTGGGGTGCCACGGCGCGCGGGGCTAGAGCGCATTGTCAGGCGCGAGGTCCTGCAGCGC CCCCTCGCGGGAATAGAACTCGTCGATCACGGCCTGCGGAATGCGCTTCAGCATCTCCTT GGGGAAGATGCGCAGCAGCTTCCAGCCCAGGTCCAGCGACTCGAACACCGAGCGGTTCTC GTAGGGGCCCTGATTGATGAAGTTCTTCTCAAACTTCTGCAGGAATTCCAGGTAGAGCAG GTCCTCAGAGGTGAGCGCCTCCTCCCCAACTACTGCCTTCATGGCCTGCACGTCCTTCCC GATGGCATAGCAGGCGTACAGCTGGTTGGAGACATCTCCATGGTCCTTTCTTGTCATGCC TTCCCCATGGCTGACTTCATCAGCCGCGACAGGGAAAGGAGCACGTTGATGGGGGGGTAG ATCTGTCTGTTGTGAAGCTGTCTGTCCACGTAGATCTGTCCCTCTGTGATGAAGCCCGTC CAGTCTGGGATAGGGTGGGTGATATCGTCCTTGGGCATGGTGAGGATGGGGGACCA |
Restriction Sites | NotI-NotI |
ACCN | NM_001692 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001692.2, NP_001683.2 |
RefSeq Size | 1956 bp |
RefSeq ORF | 1956 bp |
Locus ID | 525 |
UniProt ID | P15313 |
Domains | ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N |
Protein Families | Druggable Genome |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209462 | ATP6V1B1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1) |
CNY 3,824.00 |
|
RC209462L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209462L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1), mGFP tagged |
CNY 5,890.00 |
|
RG209462 | ATP6V1B1 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1) |
CNY 5,424.00 |
其它ATP6V1B1产品