NDST1 (NM_001543) Human Untagged Clone

CAT#: SC119198

NDST1 (untagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1)



  "NM_001543" in other vectors (4)

CNY 17,010.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
NDST1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HSST; MRT46; NST1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001543, the custom clone sequence may differ by one or more nucleotides


ATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGTCCCCGCAGGCTGTCCTTTTCCTGCTGT
TCATCTTCTGCCTGTTCAGCGTTTTCATCTCGGCCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCC
CTCGGCGGATGCCCCCGAGCCTGACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTC
AAGCCTGTGCAGGCAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACT
CGCAACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGATTGCGCC
GGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCCTCATCATCTATGAGAAC
ATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGCTGGACAAGTACTGTGTGGCCTACGGCG
TGGGCATCATTGGCTTCTTCAAGGCCAATGAGAACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCT
GTTCCTGCACTCAAACCTGGGCCTGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACG
CGACCTAGCGAGGTGGAGAAAGGTGTGCTCCCCGGCGAGGACTGGACGGTTTTCCAGTCAAATCACTCCA
CCTATGAGCCAGTGCTGCTGGCCAAGACGCGCTCGTCTGAGTCCATCCCACACCTGGGCGCAGACGCCGG
CCTGCATGCTGCACTGCACGCCACTGTGGTCCAGGACCTGGGCCTGCACGACGGCATCCAGCGCGTGCTG
TTTGGCAACAACCTGAACTTCTGGCTGCACAAGCTTGTCTTCGTGGATGCCGTGGCCTTCCTCACGGGGA
AGCGCCTCTCCCTGCCATTGGACCGCTACATCCTGGTGGACATTGATGACATCTTCGTGGGCAAGGAGGG
CACACGCATGAAGGTGGAGGACGTGAAGGCCCTGTTTGACACACAGAACGAACTACGCGCACACATCCCA
AACTTCACCTTCAACCTGGGCTACTCAGGGAAATTCTTCCACACAGGTACCAATGCTGAGGACGCTGGGG
ATGATCTGCTGCTGTCGTATGTGAAGGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCCCA
CCTTTTCCACAACCAGTCCGTGTTGGCCGAGCAGATGGCCTTGAACAAGAAGTTCGCTGTCGAGCATGGC
ATTCCCACAGACATGGGGTATGCAGTGGCGCCCCACCACTCGGGCGTGTACCCCGTGCACGTGCAGCTGT
ACGAGGCTTGGAAGCAGGTGTGGAGCATCCGCGTGACCAGCACGGAGGAGTACCCCCACCTGAAGCCAGC
CCGCTACCGCCGTGGCTTCATCCACAATGGCATCATGGTTCTCCCACGGCAGACCTGCGGCCTCTTCACA
CACACCATCTTCTACAACGAGTACCCTGGCGGCTCCAGTGAGCTGGACAAGATCATCAACGGGGGCGAGC
TCTTCCTCACCGTGCTCCTCAATCCTATCAGCATCTTCATGACGCACCTGTCCAACTATGGGAATGACCG
CCTGGGCCTGTACACCTTCAAGCACCTGGTGCGCTTCCTGCACTCCTGGACGAACCTCCGGCTGCAGACA
CTGCCCCCTGTGCAGTTGGCGCAGAAGTACTTCCAGATCTTCTCCGAGGAGAAGGACCCGCTCTGGCAGG
ACCCCTGCGAGGACAAACGTCACAAAGACATCTGGTCCAAGGAGAAGACGTGTGACCGCTTCCCAAAGCT
CCTCATCATCGGCCCCCAGAAAACAGGCACCACTGCCCTCTACCTGTTCCTGGGCATGCACCCTGACCTA
AGCAGCAACTACCCCAGCTCTGAGACCTTTGAGGAGATCCAGTTTTTTAATGGCCACAACTATCACAAAG
GCATCGACTGGTACATGGAGTTCTTCCCCATCCCTTCCAACACCACCTCCGACTTCTACTTTGAGAAAAG
CGCCAACTACTTTGATTCAGAAGTGGCGCCCCGGCGGGCAGCAGCCCTCTTGCCCAAAGCCAAGGTCCTG
ACCATCCTCATCAACCCCGCGGACCGGGCCTATTCCTGGTACCAGCACCAGCGAGCCCATGACGACCCAG
TGGCCCTAAAGTACACCTTCCATGAGGTGATTACCGCCGGCTCTGACGCATCCTCGAAGCTGCGTGCCCT
CCAGAACCGCTGCCTGGTCCCTGGCTGGTACGCCACCCACATCGAGCGCTGGCTCAGTGCCTATCACGCC
AACCAGATTCTGGTCTTGGATGGCAAACTGCTTCGCACAGAACCTGCCAAAGTGATGGACATGGTGCAGA
AGTTCCTTGGGGTGACCAACACCATTGACTACCACAAAACCTTGGCGTTTGATCCAAAGAAAGGATTTTG
GTGCCAACTGCTTGAAGGAGGAAAAACCAAGTGTCTGGGCAAAAGCAAGGGCCGGAAATATCCCGAGATG
GACTTGGATTCCCGAGCCTTCCTGAAGGACTATTACCGGGACCACAACATCGAGCTCTCCAAGCTGCTGT
ATAAGATGGGCCAGACACTTCCCACTTGGCTACGAGAGGACCTCCAGAACACCAGGTAG


>OriGene 5' read for NM_001543 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCTCCGGTGGCCA
AGGTCTCGGAGGCCAGGATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGT
CCCCGCAGGCTGTCCTTTTCCTGCTGTTCATCTTCTGCCTGTTCAGCGTTTTCATCTCGG
CCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCCCTCGGCGGATGCCCCCGAGCCTG
ACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTCAAGCCTGTGCAGG
CAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACTCGC
AACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGA
TTGCGCCGGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCC
TCATCATCTATGAGAACATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGC
TGGACAAGTACTGTGTGGCCTACGGCGTGGGCATCATTGGCTTCTTCAAGGCCAATGAGA
ACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCTGTTCCTGCACTCAAACCTGNGCC
TGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACGCGACCTANCGAGG
TGGAGAAAAGGTGTGCTCCCCGGCCGAGACTGGNACGGNTTTTNCAGTCAAATCACTCCA
CCCTATGAGCAGNTGCTGCTGGCCAAGACGCGCCTCGTCTGAGTCCATCCACACCTGGGC
GCANACGCCNGCCTGCATGCTGACTGACGCCACTGTGGTCANGACCCTGGCTGNACGACG
CTTCCAAGGNGTGTGGTTGTTGGCACAACCTGACTCTGCTGACN
>OriGene 3' read for NM_001543 unedited
NNGTACTTGNACCCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTATTTTAGC
TTCTTAGTATTTGGGAGGTTAAGGGGGTCTCTGAGAGCCCTCGGCCACAGGTGGCTAAGC
AAAGCCGTGGCTGGCAGTCTCACCAGCTCGCCCATGTCTGCCTGGGCCTGAGGCTGGGAG
GGTGTGGGGACCGTGTCTGTACATGGGAACATGTTACTGGCAGACCCCTGGCCTGCCTCA
GTCTTTTACCACAGGAAACACCACACATCATATCCCAGGGGGAAGAAAGGGGTGCAGGTG
GGGCCCGGAGAACTGGGCCCGATGGCAAGGCAGTGCCCCAAGGCCCTGCGGGAAGCCTTG
AGGATGGAGTGGATACTGTAGGAACCAGAAATTCTTCCCCAGAACGGGAGGGTGAGATGT
GAAGGTAGGAACCAGATGTGGAGGCTGGGAGTCCTCTTGAGGGGGCCACCCTCCCATCCA
GCCTAGGACTGACTCCGGGGACCCAGCTGGCCCCTTGGAAGAGCCTGGCTCGAAACAGGG
AAATGTCCAGTCTGTCTCCCCCGGCCAGGCTCTCCTCCCCTACAGCCTGGAGGACAGGGA
CATATGGCAGCGAATACTGAGGGGCGCCCTGCGNAAGCAGGGAGTGATGGGTGACAANAG
GGGACAGTCGGCNGAACACAGTGAGGGACAGAAAAGAGTCTCGTGGACTCCCTCCTACCA
GAAAGCGGCCTCCCCAGGAGCACCTGGGAACGAATGAACCCCTGCTGGCGAGGGAGGTCC
CTTAGGCGCAAACCAGCGTGGGTGCTCCGAGGGGCTGCAAATCGCCTGGGTGCTCCCCAC
CCCACCAAACCTTCCAAAATATGGTCATAATGCGCCATTGCCCTGTCCAGTTTCCATTTT
GAGGTTTGGTAACGGTGGGGGGATTGC
Restriction Sites NotI-NotI     
ACCN NM_001543
Insert Size 3670 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001543.3, NP_001534.1
RefSeq Size 7913 bp
RefSeq ORF 2649 bp
Locus ID 3340
UniProt ID P52848
Domains Sulfotransfer
Protein Families Transmembrane
Protein Pathways Heparan sulfate biosynthesis, Metabolic pathways
Gene Summary This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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