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Non Neuronal Enolase (ENO1) (NM_001428) Human Untagged Clone
CAT＃: SC119247
ENO1 (untagged)-Human enolase 1, (alpha) (ENO1), transcript variant 1
CNY 3,656.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENO1L1; HEL-S-17; MPB1; NNE; PPH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001428, the custom clone sequence may differ by one or more nucleotides
ATGTCTATTCTCAAGATCCATGCCAGGGAGATCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATC TCTTCACCTCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCCCT AGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT AAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAACAAGAGAAGATTGACAAACTGA TGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTTGGTGCGAACGCCATTCTGGGGGTGTCCCTTGC CGTCTGCAAAGCTGGTGCCGTTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAAC TCTGAAGTCATCCTGCCAGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGG CCATGCAGGAGTTCATGATCCTCCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGCAGA GGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAATATGGGAAAGATGCCACCAATGTGGGGGATGAA GGCGGGTTTGCTCCCAACATCCTGGAGAATAAAGAAGGCCTGGAGCTGCTGAAGACTGCTATTGGGAAAG CTGGCTACACTGATAAGGTGGTCATCGGCATGGACGTAGCGGCCTCCGAGTTCTTCAGGTCTGGGAAGTA TGACCTGGACTTCAAGTCTCCCGATGACCCCAGCAGGTACATCTCGCCTGACCAGCTGGCTGACCTGTAC AAGTCCTTCATCAAGGACTACCCAGTGGTGTCTATCGAAGATCCCTTTGACCAGGATGACTGGGGAGCTT GGCAGAAGTTCACAGCCAGTGCAGGAATCCAGGTAGTGGGGGATGATCTCACAGTGACCAACCCAAAGAG GATCGCCAAGGCCGTGAACGAGAAGTCCTGCAACTGCCTCCTGCTCAAAGTCAACCAGATTGGCTCCGTG ACCGAGTCTCTTCAGGCGTGCAAGCTGGCCCAGGCCAATGGTTGGGGCGTCATGGTGTCTCATCGTTCGG GGGAGACTGAAGATACCTTCATCGCTGACCTGGTTGTGGGGCTGTGCACTGGGCAGATCAAGACTGGTGC CCCTTGCCGATCTGAGCGCTTGGCCAAGTACAACCAGCTCCTCAGAATTGAAGAGGAGCTGGGCAGCAAG GCTAAGTTTGCCGGCAGGAACTTCAGAAACCCCTTGGCCAAGTAA >OriGene 5' read for NM_001428 unedited
ATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCGCGGAAAGTATCTGTGGGTA CCCGGAGCCGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTCCGCT TTCCTCTCCTAGGCGACGAGACCCAGTGGCTAGAAGTTCACCATGTCTCTTCTCAAGCTC CATGCCAGGGAGCTCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATCTCTTCACC TCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCC CTAGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTT GAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAA CAAGAGAAGATTGACAAACTGATGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTT GGTGCGAACGCCATTCTGGGGNGTGTCCCTTGCCGTCTGCAAAGCTGGTGCCGTTGAGAA GGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAACTCTGAAGTCATCCTGCC AGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGGCCATGCA GGAGTTCATGATCCTNCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGC AGAGGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAAATGGGAANAGAGCCACCAAT GTGGGGGATGAAGGGCGGGTTTGCTCCCAACATNNCTGAGATAAAGAANGNCTGNNACTG CTGAAGACTGCTATTGGGAAAACTGGCTAACTGAATAAGGTGGCAATCGCATGGACGTAC CGCCTTCGATTTTTTTAGGTTGGGGAATTAGACCTTGATTTAAACTA >OriGene 3' read for NM_001428 unedited
NNTTTTTACTCTGGCCCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTCTCAGGGGCCACTGAGGCTTTTTATTTTGAGCACA AAACCACCGGGGATCTACCCTGGGGCCACCCCGGAAATGACACGAGGCTCACATGACTCT AAACACTTGGGGGAAAGGGAGGCGAAAAAAACAATGACTTGGGCCAATTACACAACTGCA AAGCTAGAGCTGCCAACAGGGCTCCAGGGAGCTTGGCTTCTGTAAAAGTTCTAAGGAAGC GGTACAAACTCCACGGGGGGGGGGCGCTAACTAGCAGGGACCCCTGCAAGTGTTGGTCGG GGGCCTCAAGCTGCCTGAGCTGACACGAGGGGAGGGGTCTGTGTACCCAACAGGTGACCG AAGGGCTTGCCTGCCCACAGTTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAA CTTACCCTTGCTGCCCAGCTCCTTTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCG CTCAGATCGGCAAGGGGCACCAGTCTTGATCTGCCCAGTGCACAGCCCCACAACCAGGTC AGCGATGAAGGTATCTTCAGTCTCCCCCGAACGATGAGACACCATGACGCCCCAACCATT GGCCTGGGCCAGCTTGCACGCCTGAAGAGACTCGGCCACGAAGCCAATCTGTTTGACTTT GACCAGGAAGCCATTGCAGGACTTTTCGTTCACGGCCTTGAAGATCCTTTTTGGTTTGGC ACTGGGAGATCATCCCCCATACCTGAATTCCGCCCTGGCTGGAACCTTTGCCAAGCTCCC AATATCCTGGAAAAGGAATCCTTAATAACCCCACTGGTAACCCTGGAAGAGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001428 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001428.2, NP_001419.1 |
| RefSeq Size | 1812 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 2023 |
| UniProt ID | P06733 |
| Domains | enolase |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation |
| Gene Summary | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA
,Baleva, M;Gowher, A;Kamenski, P;Tarassov, I;Entelis, N;Masquida, B;,
Int J Mol Sci
,PubMed ID 25918939
[ENO1]
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The 1p-encoded protein stathmin and resistance of malignant gliomas to nitrosoureas
,TT Ngo, T Peng, XJ Liang, O Akeju, S Pastorino, W Zhang, Y Kotliarov, JC Zenklusen, HA Fine, D Maric, PY Wen, U De Girolami, PM Black, WW Wu, RF Shen, NO Jeffries, DW Kang, and JK Park,
J Natl Cancer Inst. 2007 Apr 18;99(8):639-52.
[ENO1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205494 | ENO1 (Myc-DDK-tagged)-Human enolase 1, (alpha) (ENO1), transcript variant 1 |
CNY 3,656.00 |
|
| RC205494L1 | Lenti ORF clone of Human enolase 1, (alpha) (ENO1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205494L2 | Lenti ORF clone of Human enolase 1, (alpha) (ENO1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205494L3 | Lenti ORF clone of Human enolase 1, (alpha) (ENO1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205494L4 | Lenti ORF clone of Human enolase 1, (alpha) (ENO1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG205494 | ENO1 (tGFP-tagged) - Human enolase 1, (alpha) (ENO1), transcript variant 1 |
CNY 5,256.00 |
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