United States
Germany
Japan
United Kingdom
China
DUSP4 (NM_001394) Human Untagged Clone
CAT＃: SC119258
DUSP4 (untagged)-Human dual specificity phosphatase 4 (DUSP4), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HVH2; MKP-2; MKP2; TYP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001394 edited
GAATTCGGCACGAGGAGGAAGGGGACAGGGAAGAAGAGGCTCTCCCGCGGGAGCCCTTGA GGACCAAGTTTGCGGCCACTTCTGCAGGCGTCCCTTCTTAGCTCTCGCCCGCCCCTTTCT GCAGCCTAGGCGGCCCGGGTTCTCTTCTCTTCCTCGCGCGCCCAGCCGCCTCGGTTCCCG GCGACCATGGTGACGATGGAGGAGCTGCGGGAGATGGACTGCAGTGTGCTCAAAAGGCTG ATGAACCGGGACGAGAATGGCGGCGGCGCGGGCGGCAGCGGCAGCCACGGCACCCTGGGG CTGCCGAGCGGCGGCAAGTGCCTGCTGCTGGACTGCAGACCGTTCCTGGCGCACAGCGCG GGCTACATCCTAGGTTCGGTCAACGTGCGCTGTAACACCATCGTGCGGCGGCGGGCTAAG GGCTCCGTGAGCCTGGAGCAGATCCTGCCCGCCGAGGAGGAGGTACGCGCCCGCTTGCGC TCCGGCCTCTACTCGGCGGTCATCGTCTACGACGAGCGCAGCCCGCGCGCCGAGAGCCTC CGCGAGGACAGCACCGTGTCGCTGGTGGTGCAGGCGCTGCGCCGCAACGCCGAGCGCACC GACATCTGCCTGCTCAAAGGCGGCTATGAGAGGTTTTCCTCCGAGTACCCAGAATTCTGT TCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTTCCCCCCAGTGCCACAGAGCCC TTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACACGACCAGGGGGGTCCTGTGGAG ATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCTGCCCGGAGAGACATGCTGGAC GCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGACTGCCCAAACCACTTTGAAGGA CACTATCAGTACAAGTGCATCCCAGTGGAAGATAACCACAAGGCCGACATCAGCTCCTGG TTCATGGAAGCCATAGAGTACATCGATGCCGTGAAGGACTGCCGTGGGCGCGTGCTGGTG CACTGCCAGGCGGGCATCTCGCGGTCGGCCACCATCTGCCTGGCCTACCTGATGATGAAG AAACGGGTGAGGCTGGAGGAGGCCTTCGAGTTCGTTAAGCAGCGCCGCAGCATCATCTCG CCCAACTTCAGCTTCATGGGGCAGCTGCTGCAGTTCGAGTCCCAGGTGCTGGCCACGTCC TGTGCTGCGGAGGCTGCTAGCCCCTCGGGACCCCTGCGGGAGCGGGGCAAGACCCCCGCC ACCCCCACCTCGCAGTTCGTCTTCAGCTTTCCGGTCTCCGTGGGCGTGCACTCGGCCCCC AGCAGCCTGCCCTACCTGCACAGCCCCATCACCACCTCTCCCAGCTGTTAGAGCCGCCCT GGGGGCCCCAGAACCAGAGCTGGCTCCCAGCAAGGGTAGGACGGGCCGCATGCGGGCAGA AAGTTGGGACTGAGCAGCTGGGAGCAGGCGACCGAGCTCCTTCCCCATCATTTCTCCTTG GCCAACGACGAGGCCAGCCAGAATGGCAATAAGGACTCCGAATACATAATAAAAGCAAAC AGAACACTCCAACTTAGAGCAATAACGGCTGCCGCAGCAGCCAGGGAAGACCTTGGTTTG GTTTATGTGTCAGTTTCACTTTTCCGATAGAAATTTCTTACCTCATTTTTTTAAGCAGTA AGGCTTGAAGTGATGAAACCCACAGATCCTAGCAAATGTGCCCAACCAGCTTTACTAAAG GGGGAGGAAGGGAGGGCAAAGGGATGAGAAGACAAGTTTCCCAGAAGTGCCTGGTTCTGT GTACTTGTCCCTTTGTTGTCGTTGTCGTAGTTAAAGGAATTTCATTTTTTAAAAGAAAAA AAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_001394 unedited
CGGCAAAAACCTTCCGCACGAGGAGGAAGGGGACAGGNGAAGAAGAGGCTCTCCCGCGGG AGCCCTTGAGGACCAAGTTTGCGGCCACTTCTGCAGGCGTCCCTTCTTAGCTCTCGCCCG CCCCTTTCTGCAGCCTATGCGGCCCGGGTTCTCTTCTCTTCCTCGCGCGCCCAGCCGCCT CGGTGCCTGGCGACCATGGTGACGATGGATGAGCTGCGGGAGATGGACTGCAGTGTGCTC AAAAGGCTGATGAACCGGGACGAGAATGGCGGCGGCGCGGGCGGCAGCGGCAGCCACGGC ACCCTGGGGCTGCCGAGCGGCGGCAAGTGCCTGCTGCTGGACTGCAGACCGTTCCTGGCG CACAGCGCGGGCTACATCCTAGGTTCGGTCAACGTGCGCTGTAACACCATCGTGCGGCGG CGGGCTAAGGGCTCCGTGAGCCTGGAGCAGATCCTGCCCGCCGAGGAGGAGGTACGCGCC CGCTTGCGCTCCGGCCTCTACTCGGCGGTCATCGTCTACGACGAGCGCAGCCCGCGCGCC GAGAGCCTCCGCGAGGACAGCACCGTGTCGCTGGTGGTGCAAGCGCTGCGCCGCATCGCC GAGCGCACCGACATCTGCCTGCTCAAAGGCGGCTATGAGAGGTTTTCCTCCGAGTACCCA GAATTCTGTGTCTAAACCAAGGCCCTGGCAGCCATCCCACCCCNCGGGTTCCCCCAGTGC CACAGAGCCCTTGGACCTTGGCTGCAGCTCCTGTGGGACCCCACTACACGACCAGGGGAG GTCCCTGTGGAGAATCCTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCCTGCCGGAGA GACTGCTGGACCCCTGGGCATACAGCTCTGTGGATGTCTCCTCGCCTGCCCA >OriGene 3' read for NM_001394 unedited
CGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCTTTTAAAAAATGAAATTC CTTTAACTACGACAACGACAACAAAGGGACAAGTACACAGAACCAGGCACTTCTGGGAAA CTTGTCTTCTCATCCCTTTGCCCTCCCTTCCTCCCCCTTTAGTAAAGCTGGTTGGGCACA TTTGCTAGGATCTGTGGGTTTCATCACTTCAAGCCTTACTGCTTAAAAAAATGAGGTAAG AAATTTCTATCGGAAAAGTGAAACTGACACATAAACCAAACCAAGGTCTTCCCTGGCTGC TGCGGCAGCCGTTATTGCTCTAAGTTGGAGTGTTCTGTTTGCTTTTATTATGTATTCGGA GTCCTTATTGCCATTCTGGCTGGCCTCGTCGTTGGCCAAGGAGAAATGATGGGGAAGGAG CTCGGTCGCCTGCTCCCAGCTGCTCAGTCCCAACTTTCTGCCCGCATGCGGCCCGTCCTA CCCTTGCTGGGAGCCAGCTCTGGTTCTGGGGCCCCCAGGGCGGCTCTAACAGCTGGGAGA GGCGGTGATGGGGCTGCGCAGGTAGGGCAGGCTGCTGGGGGCCGAGTGCCCGCCCACGGA GACCGGATAGCTGAAGACCAACTGCGAGGTGGGGGTGGCGGGGGCCTTGCCCCGCTCCCC AGGGGTCCCGAGGGGCTAGCAGACCTCGCAGCACAGGACGTGGCCAGCACCTGGGACTCG AACTGCAGCAGTTGCCCCAGAAGCTGAAGTTGGGCGAAATGATGCTGCGGCGCTGTTAAC CAACTCGAAGCCACCTCAACCTCACCCGTTTCTCATTATCAGTAGCCAAGCAAATGGGGC CGACGCGATAGCCCGGCTGGCATGCACCACCACGCCCCCCGGGAGACTTCCGGCACGAGG ACCCTATGGTCCAAGAACAAGAACGAAGNCGGCCTGAGGTATTCTCCCTGGGAGGCCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001394 |
| Insert Size | 1850 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001394.5, NP_001385.1 |
| RefSeq Size | 2498 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 1846 |
| UniProt ID | Q13115 |
| Domains | DSPc, RHOD, PTPc_motif |
| Protein Families | Phosphatase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibition of gluconeogenesis through transcriptional activation of EGR1 and DUSP4 by AMPK
,Stephen P. Berasi, Christine Huard, Dongmei Li, Heather H. Shih, Ying Sun, Wenyan Zhong, Janet E. Paulsen, Eugene L. Brown, Ruth E. Gimeno, and Robert V. Martinez,
J Biol Chem. 2006;281:27167-27177
[DUSP4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200640 | DUSP4 (Myc-DDK-tagged)-Human dual specificity phosphatase 4 (DUSP4), transcript variant 1 |
CNY 5,488.00 |
|
| RC200640L1 | Lenti ORF clone of Human dual specificity phosphatase 4 (DUSP4), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200640L2 | Lenti ORF clone of Human dual specificity phosphatase 4 (DUSP4), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC200640L3 | Lenti ORF clone of Human dual specificity phosphatase 4 (DUSP4), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200640L4 | Lenti ORF clone of Human dual specificity phosphatase 4 (DUSP4), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG200640 | DUSP4 (tGFP-tagged) - Human dual specificity phosphatase 4 (DUSP4), transcript variant 1 |
CNY 7,088.00 |
