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DKC1 (NM_001363) Human Untagged Clone
CAT＃: SC119282
DKC1 (untagged)-Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1
CNY 3,832.00
| Cited in 2 publications. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CBF5; DKC; DKCX; NAP57; NOLA4; XAP101 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119282 sequence for NM_001363 edited (data generated by NextGen Sequencing)
ATGGCGGATGCGGAAGTAATTATTTTGCCAAAGAAACATAAGAAGAAAAAGGAGCGGAAG TCATTGCCAGAAGAAGATGTAGCCGAAATACAACACGCTGAAGAATTTCTTATCAAACCT GAATCCAAAGTTGCTAAGTTGGACACGTCTCAGTGGCCCCTTTTGCTAAAGAATTTTGAT AAGCTGAATGTAAGGACAACACACTATACACCTCTTGCATGTGGTTCAAATCCTCTGAAG AGAGAGATTGGGGACTATATCAGGACAGGTTTCATTAATCTTGACAAGCCCTCTAACCCC TCTTCCCATGAGGTGGTAGCCTGGATTCGACGGATACTTCGGGTGGAGAAGACAGGGCAC AGTGGTACTCTGGATCCCAAGGTGACTGGTTGTTTAATCGTGTGCATAGAACGAGCCACT CGCTTGGTGAAGTCACAACAGAGTGCAGGCAAAGAGTATGTGGGGATTGTCCGGCTGCAC AATGCTATTGAAGGGGGGACCCAGCTTTCTAGGGCCCTAGAAACTCTGACAGGTGCCTTA TTCCAGCGACCCCCACTTATTGCTGCAGTAAAGAGGCAGCTCCGAGTGAGGACCATCTAC GAGAGCAAAATGATTGAATACGATCCTGAAAGAAGATTAGGAATCTTTTGGGTGAGTTGT GAGGCTGGCACCTACATTCGGACATTATGTGTGCACCTTGGTTTGTTATTGGGAGTTGGT GGTCAGATGCAGGAGCTTCGGAGGGTTCGTTCTGGAGTCATGAGTGAAAAGGACCACATG GTGACAATGCATGATGTGCTTGATGCTCAGTGGCTGTATGATAACCACAAGGATGAGAGT TACCTGCGGCGAGTTGTTTACCCTTTGGAAAAGCTGTTGACATCTCATAAACGGCTGGTT ATGAAAGACAGTGCAGTAAATGCCATCTGCTATGGGGCCAAGATTATGCTTCCAGGTGTT CTTCGATATGAGGACGGCATTGAGGTCAATCAGGAGATTGTGGTTATCACCACCAAAGGA GAAGCAATCTGCATGGCTATTGCATTAATGACCACAGCGGTCATCTCTACCTGCGACCAT GGTATAGTAGCCAAGATCAAGAGAGTGATCATGGAGAGAGACACTTACCCTCGGAAGTGG GGTTTAGGTCCAAAGGCAAGTCAGAAGAAGCTGATGATCAAGCAGGGCCTTCTGGACAAG CATGGGAAGCCCACAGACAGCACACCTGCCACCTGGAAGCAGGAGTATGTTGACTACAGT GAGTCTGCCAAAAAAGAGGTGGTTGCTGAAGTGGTAAAAGCCCCGCAGGTAGTTGCCGAA GCAGCAAAAACTGCGAAGCGGAAGCGAGAGAGTGAGAGTGAAAGTGACGAGACTCCTCCA GCAGCTCCTCAGTTGATCAAGAAGGAAAAGAAGAAGAGTAAGAAGGACAAGAAGGCCAAA GCTGGTCTGGAGAGCGGGGCCGAGCCTGGAGATGGGGACAGTGATACCACCAAGAAGAAG AAGAAGAAGAAGAAAGCAAAAGAGGTAGAATTGGTTTCTGAGTAG Clone variation with respect to NM_001363.3 >OriGene 5' read for NM_001363 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTGTGGACCGGGCGGC ACGCACGCGGTGCAGGGTAACATGGCGGATGCGGAAGTAATTATTTTGCCAAAGAAACAT AAGAAGAAAAAGGAGCGGAAGTCATTGCCAGAAGAAGATGTAGCCGAAATACAACACGCT GAAGAATTTCTTATCAAACCTGAATCCAAAGTTGCTAAGTTGGACACGTCTCAGTGGCCC CTTTTGCTAAAGAATTTTGATAAGCTGAATGTAAGGACAACACACTATACACCTCTTGCA TGTGGTTCAAATCCTCTGAAGAGAGAGATTGGGGACTATATCAGGACAGGTTTCATTAAT CTTGACAAGCCCTCTAACCCCTCTTCCCATGAGGTGGTAGCCTGGATTCGACGGATACTT CGGGTGGAGAAGACAGGGCACAGTGGTACTCTGGATCCCAAGGTGACTGGTTGTTTAATC GTGTGCATAGAACGAGCCACTCGCTTGGTGAAGTCACAACAGAGTGCAGCAAAGAGTATG TGGGGATTGTCCGGCTGCACAATGCTATTGAAGGGGGGACCCAGCTTTCTAGGGCCCTAG AAACTCTGACAGGTGCCTTATTCCAGCGACCCCCACTTATTGCTGCAGTAAAGAGGCAGC TCCGAGTGAGGACCATCTACGAGAGCAAATGATTGAATACGATCCTGAAAGAAATTANGA ATCTTTTGGNGGTGAGTGTGAGGCTGGCACCTCATTCGACATTATGTGTGCACCTTGGTT NGTATTGGAAANTTGTGGGCAATGCAGACTTTNNNAGGGGGTCGTTCTGAGTCATAGTGA AAGGACACATGTGACAGCCTGAGTGCTGAGGCTATGGCTGATGAACACAGGAGAAAGTCT GGGGCGATTGTTACTTGGAAAGCGTGATTCTAACGCGGTTT >OriGene 3' read for NM_001363 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTCAAATAGGACATA AAGGTCCCTAAATTTTTAATCAAAATATTTTACAATTTTTGCTCTGCTAGCATGAGATCA ACAAGAAAACCCAACAGGAATACATAAAAAACTTAACAAGTTGACTTTAATAAGTAAAAA TTACAACATTCTTCTCCCAGCAATGAACAATTTCATTTTTTAAAAGTAAACGAGGCCTAA AACAAGAGGGCACAGGAACAAGTAGTCAGATGGATTTAGGTGAGCACTGTACACAAGCTT TGAGGAAGTGCAAAGGACTGACCTCTAGGCCAGAACAAGATGGAAAACTACCAGGCCCAT CGGGCCTATAACCCAGACACCAGCATGGACAAAACTCAGTTATACTGAATTCAGAGACAA AATTCAGTGACACTCTTCTACCACTTATTTAGGGTTCTACAGCATTTCACTGAGCAGACT TAGTTTTTTGTTTTTGTTTTACAAACCTTTTAAACAGAGGGGATTTAAAAAATGCAAACT AGGACTACGATGTTAAGACAACCACTAGCAGACAGCTGCGGACAGTTACTGGGTCTGAAG GTGAGGCTTCCCAACTCAAACAGAGAAGTCATTGGGATAAACTCTGCCTCTTTCATCTTT CACCACCCATATCCTTAAAACAGGACAAGATGGGATGACCACACCAGGTAACATCACCGA GGTCTCAAAGTAGCAGCTAAAATGTGCCAGAACTCTTCACCCTGTCTAGGACCTATGTTC CACTCTCTCAGCAACAAAAGGATCTATAACATGGGTTTCTCAATAAGGGCTTTAGTTTCT CCTCCAGCTTCAAGTGGCCTTCACTACTCAGAACCCNANTNCTACCTCTTNGGGCTTCCT CTCTTCTTCTTCTCTTGGGGGTATCACTGGNCCCATTTCCAGGCTCGGGCCCGCTCTCAA AACAGATTTGCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001363 |
| Insert Size | 2450 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001363.2, NP_001354.1 |
| RefSeq Size | 2465 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 1736 |
| UniProt ID | O60832 |
| Domains | PUA, TruB_N |
| Protein Families | Druggable Genome |
| Gene Summary | This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Telomeric G-quadruplexes are a substrate and site of localization for human telomerase
,Moye, AL;Porter, KC;Cohen, SB;Phan, T;Zyner, KG;Sasaki, N;Lovrecz, GO;Beck, JL;Bryan, TM;,
Nat Commun
,PubMed ID 26158869
[DKC1]
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Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients
,Suneet Agarwal, Yuin-Han Loh, Erin M. McLoughlin, Junjiu Huang, In-Hyun Park, Justine D. Miller, Hongguang Huo, Maja Okuka, Rosana Maria dos Reis, Sabine Loewer, et al.,
Nature (17 February 2010) doi:10.1038/nature08792 Letter
[DKC1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201455 | DKC1 (Myc-DDK-tagged)-Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1 |
CNY 3,832.00 |
|
| RC201455L1 | Lenti ORF clone of Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1, Myc-DDK-tagged |
CNY 6,232.00 |
|
| RC201455L2 | Lenti ORF clone of Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201455L3 | Lenti ORF clone of Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201455L4 | Lenti ORF clone of Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201455 | DKC1 (tGFP-tagged) - Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1 |
CNY 5,432.00 |
|
| SC320317 | DKC1 (untagged)-Human dyskeratosis congenita 1, dyskerin (DKC1), transcript variant 1 |
CNY 3,832.00 |
