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VCAM1 (NM_001078) Human Untagged Clone
CAT＃: SC119467
VCAM1 (untagged)-Human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1
CNY 8,128.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD106; INCAM-100 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119467 sequence for NM_001078 edited (data generated by NextGen Sequencing)
ATGCCTGGGAAGATGGTCGTGATCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCA GCTTCTCAAGCTTTTAAAATCGAGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGT GACTCCGTCTCATTGACTTGCAGCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGA ACCCAGATAGATAGTCCACTGAATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTG ACAATGAATCCTGTTAGTTTTGGGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAA TCTAGGAAATTGGAAAAAGGAATCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAG ATTCATTTGAGTGGCCCTCTGGAGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCT GATGTATACCCATTTGACAGGCTGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAG AGTCAGGAATTTCTGGAGGATGCAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTA ACCTTTACTCCTGTCATTGAGGATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACAC ATTGATGAAATGGATTCTGTGCCCACAGTAAGGCAGGCTGTAAAAGAATTGCAAGTCTAC ATATCACCCAAGAATACAGTTATTTCTGTGAATCCATCCACAAAGCTGCAAGAAGGTGGC TCTGTGACCATGACCTGTTCCAGCGAGGGTCTACCAGCTCCAGAGATTTTCTGGAGTAAG AAATTAGATAATGGGAATCTACAGCACCTTTCTGGAAATGCAACTCTCACCTTAATTGCT ATGAGGATGGAAGATTCTGGAATTTATGTGTGTGAAGGAGTTAATTTGATTGGGAAAAAC AGAAAAGAGGTGGAATTAATTGTTCAAGAGAAACCATTTACTGTTGAGATCTCCCCTGGA CCCCGGATTGCTGCTCAGATTGGAGACTCAGTCATGTTGACATGTAGTGTCATGGGCTGT GAATCCCCATCTTTCTCCTGGAGAACCCAGATAGACAGCCCTCTGAGCGGGAAGGTGAGG AGTGAGGGGACCAATTCCACGCTGACCCTGAGCCCTGTGAGTTTTGAGAACGAACACTCT TATCTGTGCACAGTGACTTGTGGACATAAGAAACTGGAAAAGGGAATCCAGGTGGAGCTC TACTCATTCCCTAGAGATCCAGAAATCGAGATGAGTGGTGGCCTCGTGAATGGGAGCTCT GTCACTGTAAGCTGCAAGGTTCCTAGCGTGTACCCCCTTGACCGGCTGGAGATTGAATTA CTTAAGGGGGAGACTATTCTGGAGAATATAGAGTTTTTGGAGGATACGGATATGAAATCT CTAGAGAACAAAAGTTTGGAAATGACCTTCATCCCTACCATTGAAGATACTGGAAAAGCT CTTGTTTGTCAGGCTAAGTTACATATTGATGACATGGAATTCGAACCCAAACAAAGGCAG AGTACGCAAACACTTTATGTCAATGTTGCCCCCAGAGATACAACCGTCTTGGTCAGCCCT TCCTCCATCCTGGAGGAAGGCAGTTCTGTGAATATGACATGCTTGAGCCAGGGCTTTCCT GCTCCGAAAATCCTGTGGAGCAGGCAGCTCCCTAACGGGGAGCTACAGCCTCTTTCTGAG AATGCAACTCTCACCTTAATTTCTACAAAAATGGAAGATTCTGGGGTTTATTTATGTGAA GGAATTAACCAGGCTGGAAGAAGCAGAAAGGAAGTGGAATTAATTATCCAAGTTACTCCA AAAGACATAAAACTTACAGCTTTTCCTTCTGAGAGTGTCAAAGAAGGAGACACTGTCATC ATCTCTTGTACATGTGGAAATGTTCCAGAAACATGGATAATCCTGAAGAAAAAAGCGGAG ACAGGAGACACAGTACTAAAATCTATAGATGGCGCCTATACCATCCGAAAGGCCCAGTTG AAGGATGCGGGAGTATATGAATGTGAATCTAAAAACAAAGTTGGCTCACAATTAAGAAGT TTAACACTTGATGTTCAAGGAAGAGAAAACAACAAAGACTATTTTTCTCCTGAGCTTCTC GTGCTCTATTTTGCATCCTCCTTAATAATACCTGCCATTGGAATGATAATTTACTTTGCA AGAAAAGCCAACATGAAGGGGTCATATAGTCTTGTAGAAGCACAGAAATCAAAAGTGTAG Clone variation with respect to NM_001078.3 2208 g=>a >OriGene 5' read for NM_001078 unedited
TCTTGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGATCGGGCCTC ACTGGCTTCAGGAGCTGAATACCCTCCCAGGCACACACAGGTGGGACACAAATAAGGGTT TTGGAACCACTATTTTCTCATCACGACAGCAACTTAAAATGCCTGGGAAGATGGTCGTGA TCCTTGGAGCCTCAAATATACTTTGGATAATGTTTGCAGCTTCTCAAGCTTTTAAAATCG AGACCACCCCAGAATCTAGATATCTTGCTCAGATTGGTGACTCCGTCTCATTGACTTGCA GCACCACAGGCTGTGAGTCCCCATTTTTCTCTTGGAGAACCCAGATAGATAGTCCACTGA ATGGGAAGGTGACGAATGAGGGGACCACATCTACGCTGACAATGAATCCTGTTAGTTTTG GGAACGAACACTCTTACCTGTGCACAGCAACTTGTGAATCTAGGAAATTGGAAAAAGGAA TCCAGGTGGAGATCTACTCTTTTCCTAAGGATCCAGAGATTCATTTGAGTGGCCCTCTGG AGGCTGGGAAGCCGATCACAGTCAAGTGTTCAGTTGCTGATGTATACCCATTTGACAGGC TGGAGATAGACTTACTGAAAGGAGATCATCTCATGAAGAGTCAGGAATTTCTGGAGGATG CAGACAGGAAGTCCCTGGAAACCAAGAGTTTGGAAGTAACCTTTACTCCTGTCATTGAGG ATATTGGAAAAGTTCTTGTTTGCCGAGCTAAATTACACATTGATGAAATGGATTCTGTGC CCACAGTAAGGCAGGCTGNTAAAGATTTGCAGTCTACATATCACCCAAGAATACAGTTAT TTCTGTGAATCCATCCCCACAAGCTGCAGAAGGTGGCTCTGTGACCCATGACCTGTNCAG CGAGGGTCTACCC >OriGene 3' read for NM_001078 unedited
CGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTAATTCCAGAATTATCACT TTACTATACAAAAAAACCTAAACATTTACAAAAGAAAATAAAAAGAAGTTGCATTTTTAG GCCACATTGGGAAAGTTGCACAGTAGTCTGATGAACAAACTTCGTGAAACATCTCCGTAC CATGCCAGTACTATACAGAGAAACCCAGTCAACCATTATACCATAGGTTGAGGTATTTAG TACCTGAAAGTCAACCCAGTGCTCCCTTTGCTTATTATTTGTCTATAAAAATATAAAACC CGGCATCTTTACAAAACCTGAAATTATTGTCAATAAACTATATAGGAATAAAATTTTGTG AAAGGGAATAAAATAGAAGAAAGATGACAAAGGTCAGCTGTCAGCAAATATTAATGTGAA ATTTTCCTAACATGCATTAAATTATAATAGTCCAAGCAACACTAGTTATTTTAACAATCT AAATATAGAGTGTTGCTATTTTAAGCAATCTTGCTATGGCATAATTTTATTTTACCTTTG TCCAAATTAGGGTATTGGATTTACAAAACAAGGGAATTCCCCCGGTTTTTTTTTTGGGGG GATCCGGTGTTTTGGGCGAAAAGTTTTTTTTTTTTTTTTCCCACAGGGGGGGAAAAGGGG CTTTTTTTTCCCGGGAAATTTTTGGGGGGGGCGCCCCCCACCCATTTGTTTCTTGCGGGG GGGGGGGGCCCCTCGGGGTGGCCCCACAAAAAGGTGGGCCCCGGGCACAAAAAAAAACGT TGCCCCCCTTCTTGTGTGGGGGCGGGCACAAAAAAAAAAACACCCCCTCCGGGGGGGGGC GCTTCCCCAACAAAACTAATTCTCCGCCGGGGGGGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001078 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001078.2, NP_001069.1 |
| RefSeq Size | 3119 bp |
| RefSeq ORF | 2220 bp |
| Locus ID | 7412 |
| UniProt ID | P19320 |
| Domains | ig, IGv, IGc2, IG |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration |
| Gene Summary | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) encodes the predominant, full-length isoform (a). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Empéripolèse des cellules de lymphomes humains Ramos par les fibroblastes
,Oualha, N;,
Thesis
[VCAM1]
|
|
Cytokine Induction of VCAM-1 but Not IL13Ra2 on Glioma Cells: A Tale of Two Antibodies
,Mahadev, V;Starr, R;Wright, SL;Martinez, C;Jensen, MC;Barish, ME;Forman, SJ;Brown, CE;,
PLoS ONE
,PubMed ID 24787244
[VCAM1]
|
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Resources
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| RC209761L4 | Lenti ORF clone of Human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1, mGFP tagged |
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| RG209761 | VCAM1 (tGFP-tagged) - Human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1 |
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