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ATP citrate lyase (ACLY) (NM_001096) Human Untagged Clone
CAT＃: SC119485
ACLY (untagged)-Human ATP citrate lyase (ACLY), transcript variant 1
CNY 7,672.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACL; ATPCL; CLATP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001096, the custom clone sequence may differ by one or more nucleotides
ATGTCGGCCAAGGCAATTTCAGAGCAGACGGGCAAAGAACTCCTTTACAAGTTCATCTGTACCACCTCAG CCATCCAGAATCGGTTCAAGTATGCTCGGGTCACTCCTGACACAGACTGGGCCCGCTTGCTGCAGGACCA CCCCTGGCTGCTCAGCCAGAACTTGGTAGTCAAGCCAGACCAGCTGATCAAACGTCGTGGAAAACTTGGT CTCGTTGGGGTCAACCTCACTCTGGATGGGGTCAAGTCCTGGCTGAAGCCACGGCTGGGACAGGAAGCCA CAGTTGGCAAGGCCACAGGCTTCCTCAAGAACTTTCTGATCGAGCCCTTCGTCCCCCACAGTCAGGCTGA GGAGTTCTATGTCTGCATCTATGCCACCCGAGAAGGGGACTACGTCCTGTTCCACCACGAGGGGGGTGTG GACGTGGGTGATGTGGACGCCAAGGCCCAGAAGCTGCTTGTTGGCGTGGATGAGAAACTGAATCCTGAGG ACATCAAAAAACACCTGTTGGTCCACGCCCCTGAAGACAAGAAAGAAATTCTGGCCAGTTTTATCTCCGG CCTCTTCAATTTCTACGAGGACTTGTACTTCACCTACCTCGAGATCAATCCCCTTGTAGTGACCAAAGAT GGAGTCTATGTCCTTGACTTGGCGGCCAAGGTGGACGCCACTGCCGACTACATCTGCAAAGTGAAGTGGG GTGACATCGAGTTCCCTCCCCCCTTCGGGCGGGAGGCATATCCAGAGGAAGCCTACATTGCAGACCTCGA TGCCAAAAGTGGGGCAAGCCTGAAGCTGACCTTGCTGAACCCCAAAGGGAGGATCTGGACCATGGTGGCC GGGGGTGGCGCCTCTGTCGTGTACAGCGATACCATCTGTGATCTAGGGGGTGTCAACGAGCTGGCAAACT ATGGGGAGTACTCAGGCGCCCCCAGCGAGCAGCAGACCTATGACTATGCCAAGACTATCCTCTCCCTCAT GACCCGAGAGAAGCACCCAGATGGCAAGATCCTCATCATTGGAGGCAGCATCGCAAACTTCACCAACGTG GCTGCCACGTTCAAGGGCATCGTGAGAGCAATTCGAGATTACCAGGGCCCCCTGAAGGAGCACGAAGTCA CAATCTTTGTCCGAAGAGGTGGCCCCAACTATCAGGAGGGCTTACGGGTGATGGGAGAAGTCGGGAAGAC CACTGGGATCCCCATCCATGTCTTTGGCACAGAGACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC CACCGGCCCATCCCCAACCAGCCACCCACAGCGGCCCACACTGCAAACTTCCTCCTCAACGCCAGCGGGA GCACATCGACGCCAGCCCCCAGCAGGACAGCATCTTTTTCTGAGTCCAGGGCCGATGAGGTGGCGCCTGC AAAGAAGGCCAAGCCTGCCATGCCACAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGCACC ACCCTCTTCAGCCGCCACACCAAGGCCATTGTGTGGGGCATGCAGACCCGGGCCGTGCAAGGCATGCTGG ACTTTGACTATGTCTGCTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGACCA CAAGCAGAAGTTTTACTGGGGGCACAAAGAGATCCTGATCCCTGTCTTCAAGAACATGGCTGATGCCATG AGGAAGCATCCGGAGGTAGATGTGCTCATCAACTTTGCCTCTCTCCGCTCTGCCTATGACAGCACCATGG AGACCATGAACTATGCCCAGATCCGGACCATCGCCATCATAGCTGAAGGCATCCCTGAGGCCCTCACGAG AAAGCTGATCAAGAAGGCGGACCAGAAGGGAGTGACCATCATCGGACCTGCCACTGTTGGAGGCATCAAG CCTGGGTGCTTTAAGATTGGCAACACAGGTGGGATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCC CAGGCAGCGTGGCCTATGTCTCACGTTCCGGAGGCATGTCCAACGAGCTCAACAATATCATCTCTCGGAC CACGGATGGCGTCTATGAGGGCGTGGCCATTGGTGGGGACAGGTACCCGGGCTCCACATTCATGGATCAT GTGTTACGCTATCAGGACACTCCAGGAGTCAAAATGATTGTGGTTCTTGGAGAGATTGGGGGCACTGAGG AATATAAGATTTGCCGGGGCATCAAGGAGGGCCGCCTCACTAAGCCCATCGTCTGCTGGTGCATCGGGAC GTGTGCCACCATGTTCTCCTCTGAGGTCCAGTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAA ACTGCAGTAGCCAAGAACCAGGCTTTGAAGGAAGCAGGAGTGTTTGTGCCCCGGAGCTTTGATGAGCTTG GAGAGATCATCCAGTCTGTATACGAAGATCTCGTGGCCAATGGAGTCATTGTACCTGCCCAGGAGGTGCC GCCCCCAACCGTGCCCATGGACTACTCCTGGGCCAGGGAGCTTGGTTTGATCCGCAAACCTGCCTCGTTC ATGACCAGCATCTGCGATGAGCGAGGACAGGAGCTCATCTACGCGGGCATGCCCATCACTGAGGTCTTCA AGGAAGAGATGGGCATTGGCGGGGTCCTCGGCCTCCTCTGGTTCCAGAAAAGGTTGCCTAAGTACTCTTG CCAGTTCATTGAGATGTGTCTGATGGTGACAGCTGATCACGGGCCAGCCGTCTCTGGAGCCCACAACACC ATCATTTGTGCGCGAGCTGGGAAAGACCTGGTCTCCAGCCTCACCTCGGGGCTGCTCACCATCGGGGATC GGTTTGGGGGTGCCTTGGATGCAGCAGCCAAGATGTTCAGTAAAGCCTTTGACAGTGGCATTATCCCCAT GGAGTTTGTGAACAAGATGAAGAAGGAAGGGAAGCTGATCATGGGCATTGGTCACCGAGTGAAGTCGATA AACAACCCAGACATGCGAGTGCAGATCCTCAAAGATTACGTCAGGCAGCACTTCCCTGCCACTCCTCTGC TCGATTATGCACTGGAAGTAGAGAAGATTACCACCTCGAAGAAGCCAAATCTTATCCTGAATGTAGATGG TCTCATCGGAGTCGCATTTGTAGACATGCTTAGAAACTGTGGGTCCTTTACTCGGGAGGAAGCTGATGAA TATATTGACATTGGAGCCCTCAATGGCATCTTTGTGCTGGGAAGGAGTATGGGGTTCATTGGACACTATC TTGATCAGAAGAGGCTGAAGCAGGGGCTGTATCGTCATCCGTGGGATGATATTTCATATGTTCTTCCGGA ACACATGAGCATGTAA >OriGene 5' read for NM_001096 unedited
AGTATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACCAGCCGGATTTTG CGGGGTTCGTCGGGCCTGTGGAAGAAGCGCCGCGCACGGACTTCGGCAGAGGTAGAGCAG GTCTCTCTGCAGCCATGTCGGCCAAGGCAATTTCAGAGCAGACGGGCAAAGAACTCCTTT ACAAGTTCATCTGTACCACCTCAGCCATCCAGAATCGGTTCAAGTATGCTCGGGTCACTC CTGACACAGACTGGGCCCGCTTGCTGCAGGACCACCCCTGGCTGCTCAGCCAGAACTTGG TAGTCAAGCCAGACCAGCTGATCAAACGTCGTGGAAAACTTGGTCTCGTTGGGGTCAACC TCACTCTGGATGGGGTCAAGTCCTGGCTGAAGCCACGGCTGGGACAGGAAGCCACAGTTG GCAAGGCCACAGGCTTCCTCAAGAACTTTCTGATCGAGCCCTTCGTCCCCCACAGTCAGG CTGAGGAGTTCTATGTCTGCATCTATGCCACCCGAGAAGGGGACTACGTCCTGTTCCACC ACGAGGGGGGTGTGGACGTGGGTGATGTGGACGCCAAGGCCCAGAAGCTGCTTGTTGGCG TGGATGAGAAACTGAATCCTGAGGACATCAAAAAACACCTGTTGGTCCACGCCCCTGAAG ACAAGAAAGAAATTCTGGCCAGTTTTATCTCCGGCCTCTTCAATTTCTACGAGGACTTGT ACTTCACCTACCTCGAGATCAATCCCCTTGTAGTGACCAAAGATGAAGTCTATGTCCTTG ACTTGGCGGCCAAAGTGGACGCCACTGCCGACTACATCTGCANAGTGAAAGTGGGGTGAC ATCGAGTTCCCTTCCCCCTCTCGGCGGGAGGCATATCCAGAGGAAGCCTACCATGCAGAC CCGATGCCAAAAGTGGGACAGCCCTGAGCTGACCTGCTTGAACCCAAGGNAGATCTGNAC CATGTGGCCG >OriGene 3' read for NM_001096 unedited
NNCATGCTGCCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTGCCTTCATTGGTACTATATAGTTTATTTAAACCCGACTATGATAATAC CGAAAAAAAACTGGGGGGGCAGCTGCCCAAAATCTTTGGGGATTACAGATGCAAAGTAGT TAGGAGTCCTTGGACCCACACTTTAGTTACAGACAGACAAATCAGCTGCCTAAATACCGA GAACATAAGACACAAGACATTTCACAGCTTTCTGCATTTCCATTTTAAATGGATGGATGT TACAACTTTACATATTAAGTTACTACTCCACATATTTTGGGACAATGGCTAAGGATGCAA TGGCCCCATCCCCCTGACATAAACAAACTTTGGTCTGCAACACAAAACATTTCAGTGGGA TACCAAACAAGCCCTTAACACGTAACATACCAAAAGATCTTTAAAAATCAAATTAATACA ATGTTTTTCATGGTATATAAGGAAAAAAAAAGGGAAGTAAAAAAAAAAAAAGAGAGACAT TGGGGGGCTTTAAATGGATAGGATCTTGAGGGCATAAATGGTTCATTCCCTTCCTCCAAG GGGAAAAAATGTTTAACTAATGGAATAAAAAAGCAACATCCTAACCCCTTCAATGAGGAA AACCCCCTCCTTGCAGTCCTTAAAAGCCTTCCACATTAAAAAATCCCCACGGCGACCACA GCAAAAGCCCAAAATATTGGGCTTGGGGTTTTTATTCTCTTGCCTTATATACAAAAATAA TGTAACTTTCTTTTTGNGGGCCTCGAAGGGCGCTCACCCCTGAGATAGTCGGTCTTCTGG GCCCTGACCCCCATAGCGTTGGTACACATTTCAGGCCCCTTGCATAACAAACACAGCACT CCCCCGCACCATTTATCTCATATCCTTTTTCTTTCGGTGAAACACAGCCTCTCTTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001096 |
| Insert Size | 3306 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001096.2, NP_001087.2 |
| RefSeq Size | 4450 bp |
| RefSeq ORF | 3306 bp |
| Locus ID | 47 |
| UniProt ID | P53396 |
| Domains | CoA_binding, ligase-CoA |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways |
| Gene Summary | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) has an alternate splice site, which results in translation initiation at a downstream AUG start codon, compared to variant 3. The resulting isoform (1) is shorter at the N-terminus, compared to isoform 3. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inactivation of ATP citrate lyase by Cucurbitacin B: A bioactive compound from cucumber, inhibits prostate cancer growth
,Gao, Y;Islam, MS;Tian, J;Lui, VW;Xiao, D;,
Cancer Lett. March 2014
,PubMed ID 24690568
[ACLY]
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Inhibition of ATP citrate lyase induces triglyceride accumulation with altered fatty acid composition in cancer cells
,Migita, T;Okabe, S;Ikeda, K;Igarashi, S;Sugawara, S;Tomida, A;Soga, T;Taguchi, R;Seimiya, H;,
Int. J. Cancer Dec 2013.
,PubMed ID 24310723
[ACLY]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200508 | ACLY (Myc-DDK-tagged)-Human ATP citrate lyase (ACLY), transcript variant 1 |
CNY 7,664.00 |
|
| RC200508L1 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, Myc-DDK-tagged |
CNY 10,064.00 |
|
| RC200508L2 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, mGFP tagged |
CNY 11,310.00 |
|
| RC200508L3 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, Myc-DDK-tagged |
CNY 10,064.00 |
|
| RC200508L4 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, mGFP tagged |
CNY 10,064.00 |
|
| RG200508 | ACLY (tGFP-tagged) - Human ATP citrate lyase (ACLY), transcript variant 1 |
CNY 9,264.00 |
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