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RPL27 (NM_000988) Human Untagged Clone
CAT＃: SC119494
RPL27 (untagged)-Human ribosomal protein L27 (RPL27)
CNY 1,200.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBA16; L27 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119494 sequence for NM_000988 edited (data generated by NextGen Sequencing)
ATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGACGCTACTCCGGA CGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCCCCTACAGCCAT GCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAG AAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACTACAATCACCTA ATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCAATAAGGATGTC TTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCAAGTTTGAAGAG AGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTTAG Clone variation with respect to NM_000988.3 >OriGene 5' read for NM_000988 unedited
GGTGCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGTGG TTGCTGCCGAAATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGAC GCTACTCCGGACGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCC CCTACAGCCATGCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCA TGGGCAAGAAGAAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACT ACAATCACCTAATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCA ATAAGGATGTCTTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCA AGTTTGAAGAGAGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTT AGATGCTTTGTTTTGATCATTAAAAATTATAAAGAAAAAAAAAAAAAAAAAACTCGACTC TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTC CTAATAAAATTAAGTTGCATCATTNTGTCTGACTAGGTGTCCTTCTATAATATTATGGGG TGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTTAGGCCTGCGGG GTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATTCTGGCTCACTGCAATCTCCCCC TCCTGGGTCCAAGCGATNCTTCTGCCTCAACCTNCCCGANTTGTTGGGATTCCAGGCTGG CTGACCAGGCCCAAGTAATTT >OriGene 3' read for NM_000988 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTATAATTTTTAATGATCAAA ACAAAGCATCTAAAACCGCAGTTTCTGGAAGAACCACTTGTTCTTGCCTGTCTTGTATCT CTCTTCAAACTTGACCTTGGCCTCCCGTCGGGCCTTGCGTTTAAGAGCAGGATCTCTGAA GACATCCTTATTGACGACAGTTTTGTCCAAGGGGATATCCACAGAGTACCTTGTGGGCAT TAGGTGATTGTAGTTATACACTTTCACAAAAGATTTTATCTTTGATCTCTTGGCGATCTT CTTCTTGCCCATGGCAGCTGTCACTTTGCGGGGGTAGCGGTCAATTCCAGCCACCAGAGC ATGGCTGTAGGGGCGATCTGAGGTGCCATCATCAATGTTCTTCACGATGACAGCTTTGCG TCCGGAGTAGCGTCCAGCCAGGACAAGCACCACCTTCCCAGGTTTCATGAACTTGCCCAT TTCGGCAGCAACCACCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACA CAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAAACCTCCCACCGTACACGCCTA CCGTCCATTTGCGTAACGGGGCCGGGTTATTACCACCTTTTGGAAAGTCCCGTTGTTTTG GTGCCAAACCAAACCTCCATTGGAGGTAATGGGGTGGAGACTCGGGAATCCCCGGAGTCA ACCGTTCCCCAGCCCCTGGGTGACTGGCACACCGGCCCCCATGGTATAGCATCACTTATA CGAAACGCCCCCCATTCGGCCAGCCCCCCAGGCGCTGATGGGTTTTATGTTTTTGGCGAT TCTACGGACACGCCGCCCAAGGGGCGCCGCGCGTACCACCCCTCATCCCCTCCACTCTTA TTCCCCGCACTTTCCCCCTACCGTCGTGCAGCCCTCTTGGTTGTTCGCGCCTTCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000988 |
| Insert Size | 510 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000988.2, NP_000979.1 |
| RefSeq Size | 513 bp |
| RefSeq ORF | 411 bp |
| Locus ID | 6155 |
| UniProt ID | P61353 |
| Domains | Ribosomal_L27e, KOW, KOW |
| Protein Families | Druggable Genome |
| Protein Pathways | Ribosome |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (1) encodes the protein. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210654 | RPL27 (Myc-DDK-tagged)-Human ribosomal protein L27 (RPL27) |
CNY 1,200.00 |
|
| RC210654L3 | Lenti ORF clone of Human ribosomal protein L27 (RPL27), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210654L4 | Lenti ORF clone of Human ribosomal protein L27 (RPL27), mGFP tagged |
CNY 5,890.00 |
|
| RG210654 | RPL27 (tGFP-tagged) - Human ribosomal protein L27 (RPL27) |
CNY 2,800.00 |
