RPL27 (NM_000988) Human Tagged ORF Clone
CAT#: RG210654
- TrueORF®
RPL27 (tGFP-tagged) - Human ribosomal protein L27 (RPL27)
ORF Plasmid: DDK
"NM_000988" in other vectors (4)
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CNY 2,800.00
CNY 4,370.00
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DBA16; L27 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210654 representing NM_000988
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGACGCTACTCCGGACGCAAAGCTG TCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCCCCTACAGCCATGCTCTGGTGGCTGGAATTGA CCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAGAAGATCGCCAAGAGATCAAAGATAAAATCT TTTGTGAAAGTGTATAACTACAATCACCTAATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAA CTGTCGTCAATAAGGATGTCTTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCAA GTTTGAAGAGAGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210654 representing NM_000988
Red=Cloning site Green=Tags(s) MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKS FVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000988 |
ORF Size | 408 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000988.5 |
RefSeq Size | 514 bp |
RefSeq ORF | 411 bp |
Locus ID | 6155 |
UniProt ID | P61353 |
Domains | Ribosomal_L27e, KOW, KOW |
Protein Families | Druggable Genome |
Protein Pathways | Ribosome |
Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210654 | RPL27 (Myc-DDK-tagged)-Human ribosomal protein L27 (RPL27) |
CNY 1,200.00 |
|
RC210654L3 | Lenti ORF clone of Human ribosomal protein L27 (RPL27), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210654L4 | Lenti ORF clone of Human ribosomal protein L27 (RPL27), mGFP tagged |
CNY 5,890.00 |
|
SC119494 | RPL27 (untagged)-Human ribosomal protein L27 (RPL27) |
CNY 1,200.00 |