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SCF (KITLG) (NM_000899) Human Untagged Clone
CAT＃: SC119596
KITLG (untagged)-Human KIT ligand (KITLG), transcript variant b
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119596 sequence for NM_000899 edited (data generated by NextGen Sequencing)
ATGAAGAAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAAT CCTCTCGTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTC ACTAAATTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGG ATGGATGTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGC TTGACTGATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATC ATAGACAAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCT AAGGATCTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTC TTTAGAATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACT AGTGATTGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACA AAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGT AATAGGAAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGGGCAGCCATGGCATTG CCAGCATTGTTTTCTCTTATAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAGA CAGCCAAGTCTTACAAGGGCAGTTGAAAATATACAAATTAATGAAGAGGATAATGAGATA AGTATGTTGCAAGAGAAAGAGAGAGAGTTTCAAGAAGTGTAA Clone variation with respect to NM_000899.4 >OriGene 5' read for NM_000899 unedited
GGGTGCAAATTATGTATACGACTCACTTAGGCGGCCGCGAAATTCGCACGAGGGAACAGC TAAACGGAGTCGCCACACCACTGTTTGTGCTGGATCGCAGCGCTGCCTTTCCTTATGAAG AAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAATCCTCTC GTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTCACTAAA TTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGGATGGAT GTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGCTTGACT GATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATCATAGAC AAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCTAAGGAT CTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTCTTTAGA ATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACTAGTGAT TGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACAAAACCA TTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTANGAATGACAGCAGTAGCAGTAATAGG AAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGNGCAGCCATGGCATTGCCAGCA TTGTTTTCTCTTATAATTGGCTNTGCTTTTGGAGCCCTATACTTGGAGAAGAGACAGCCA AGTCTTACAAGGGCAGTTGAAATATACCAATAATGAAGAGGATATGAGATAAGTTGTTGC AAGAAAAGAGAGAGAGTTCAAGAGTGTTATGTG >OriGene 3' read for NM_000899 unedited
GGTTTAACTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTGAGAGTGCATAAAATTCTTTATTTAGAACAATAAAGTATATGTATATAT CATGTAATTTTAAATTCAAACCTGTATAAAAAAGGGTTGGGACATACACCTATTTTAAAA AGTACACATACCGTATATACACATACCCATCACATTTTACAACCTTTTTTATTTAATTAA ATTTCAGTCATAATAGAACTATTTAAAATAGTGTCGACATACATTTACATTATTATATGC AAACTCCAATCTATGACTGAACTGAGGGTATATCTGCGCATCCATCTAAATTAATACTTT TCAAGTCCATTGATTTAAAAAAATGGTGGCAAGTGGACACTATAATCACATATTAACCCT TAGAATATAGAATAGAATATTTTGATTGGAAAATTATATTTAGTGAGTATTGTAGCTCAA AGATCAAGTACTGGAAAACGGTGTGGTTTTTAGTTTAACAGTTGTTTTAATTTTATTGTT AAAAACATCATAAGGATGTAGTTGGAGATGGCAGTTATAAATAAATTTACAATGCTTAGT AAACATTTCAGGATCACAAATATCCATTTTAGCATGGATTATTTCTTGAAAAAAACACCT TGTTATAAAATGAAAGTACCATTATTTTATTTTTTACAACAAAAGCATTTCAAGACTAGT TAATGGGAAGCAGCACCTTATGAATTGGTGATCAAACTCACCAATGAACTATTAGAATTT CAATCCGAATGTTTTATTTTAAAGCCCATTTCAAATAATATTGTTTCAAAGAAAAACTGG TTACTTTAAATTCTTAAACCCTTTCCCATTTTAAAGGGAATTTAAGAAATCACCCTTTTA AATGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000899 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000899.3, NP_000890.1 |
| RefSeq Size | 5435 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 4254 |
| UniProt ID | P21583 |
| Domains | SCF |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer |
| Gene Summary | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (b) encodes the longer isoform (b) which contains the primary proteolytic-cleavage site; as a result, isoform b is largely a soluble product. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Comparison of cord blood CD34 + stem cell expansion in coculture with mesenchymal stem cells overexpressing SDF-1 and soluble /membrane isoforms of SCF.
,null,
Journal of cellular biochemistry
,PubMed ID 31099108
[KITLG]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216131 | KITLG (Myc-DDK-tagged)-Human KIT ligand (KITLG), transcript variant b |
CNY 2,400.00 |
|
| RC216131L1 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant b, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216131L2 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
| RC216131L3 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant b, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216131L4 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
| RG216131 | KITLG (tGFP-tagged) - Human KIT ligand (KITLG), transcript variant b |
CNY 4,000.00 |
