HSD3B1 (NM_000862) Human Untagged Clone
CAT#: SC119611
HSD3B1 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1)
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC119611 sequence for NM_000862 edited (data generated by NextGen Sequencing)
ATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTTTCTGGGACAGAGGATCATC CGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGTCTTGGACAAGGCCTTCGGA CCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGACCAAGCTGACAGTGCTGGAA GGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATC CACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAGAGAGTCTATCATGAATGTC AATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCAAGCTAGTGTGCCAGTCTTC ATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTACAAGGAAATCATCCAGAAT GGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCCATACCCACACAGCAAAAAG CTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCTGAAAAACGGCGGCACCCTG TACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGGAAGCCGATTCCTTTCTGCT AGTATAAACGAGGCCCTGAACAACAATGGGATCCTGTCAAGTGTTGGAAAGTTCTCCACT GTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATTCTGGCCTTGAGGGCCCTG CAGGACCCCAAGAAGGCCCCAAGCATCCGAGGACAGTTCTACTATATCTCAGATGACACG CCTCACCAAAGCTATGATAACCTTAATTACACCCTGAGCAAAGAGTTCGGCCTCCGCCTT GATTCCAGATGGAGCTTTCCTTTATCCCTGATGTATTGGATTGGCTTCCTGCTGGAAATA GTGAGCTTCCTACTCAGGCCAATTTACACCTATCGACCGCCCTTCAACCGCCACATAGTC ACATTGTCAAATAGCGTATTCACCTTCTCTTATAAGAAGGCTCAGCGAGATTTGGCGTAT AAGCCACTCTACAGCTGGGAGGAAGCCAAGCAGAAAACGGTGGAGTGGGTTGGTTCCCTT GTGGACCGGCACAAGGAGAACCTGAAGTCCAAGACTCAGTGA Clone variation with respect to NM_000862.2 1012 c=>t;1100 c=>a >OriGene 5' read for NM_000862 unedited
GGGCGGTCATAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCCCTC TCCGGGTCACCCTAGNATCAGATCTGCTCCCCAGCATCTTCTGTTTCCTGGTGAGTGATT CCTGCTACTTTGGATGGCCATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTT TCTGGGACAGAGGATCATCCGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGT CTTGGACAAGGCCTTCGGACCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGAC CAAGCTGACAGTGCTGGAAGGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCA GGACGTCTCGGTCATCATCCACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAG AGAGTCTATCATGAATGTCAATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCA AGCTAGTGTGCCAGTCTTCATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTA CAAGGAAATCATCCAGAATGGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCC ATACCCACACAGCAAAAAGCTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCT GAAAAACGGCGGCACCCTGTACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGG AAGCCGATTCCTTTCTGCTAGTATAAACGAGGCCCTGAACACAATGGGATCCTGTCAAGT GTNGGAAAAGTCTCCACTGTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATT CTGGCCTTGAGGNCCCCCTGCAGACCCCAGAAGGGCCCAGCATNNCGAGACAGTTCTACT ATATCTCAGATGACACGCCTCACCAAAGCT |
Restriction Sites | NotI-NotI |
ACCN | NM_000862 |
Insert Size | 1750 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_000862.1, NP_000853.1 |
RefSeq Size | 1665 bp |
RefSeq ORF | 1122 bp |
Locus ID | 3283 |
UniProt ID | P14060 |
Domains | 3Beta_HSD |
Protein Families | Transmembrane |
Protein Pathways | Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways |
Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204497 | HSD3B1 (Myc-DDK-tagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1) |
CNY 3,656.00 |
|
RC204497L1 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC204497L2 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), mGFP tagged |
CNY 5,890.00 |
|
RC204497L3 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204497L4 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), mGFP tagged |
CNY 5,890.00 |
|
RG204497 | HSD3B1 (tGFP-tagged) - Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1) |
CNY 5,256.00 |