HSD3B1 (NM_000862) Human Untagged Clone

CAT#: SC119611

HSD3B1 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1)



  "NM_000862" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-HSD3B1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119611 sequence for NM_000862 edited (data generated by NextGen Sequencing)
ATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTTTCTGGGACAGAGGATCATC
CGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGTCTTGGACAAGGCCTTCGGA
CCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGACCAAGCTGACAGTGCTGGAA
GGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATC
CACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAGAGAGTCTATCATGAATGTC
AATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCAAGCTAGTGTGCCAGTCTTC
ATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTACAAGGAAATCATCCAGAAT
GGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCCATACCCACACAGCAAAAAG
CTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCTGAAAAACGGCGGCACCCTG
TACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGGAAGCCGATTCCTTTCTGCT
AGTATAAACGAGGCCCTGAACAACAATGGGATCCTGTCAAGTGTTGGAAAGTTCTCCACT
GTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATTCTGGCCTTGAGGGCCCTG
CAGGACCCCAAGAAGGCCCCAAGCATCCGAGGACAGTTCTACTATATCTCAGATGACACG
CCTCACCAAAGCTATGATAACCTTAATTACACCCTGAGCAAAGAGTTCGGCCTCCGCCTT
GATTCCAGATGGAGCTTTCCTTTATCCCTGATGTATTGGATTGGCTTCCTGCTGGAAATA
GTGAGCTTCCTACTCAGGCCAATTTACACCTATCGACCGCCCTTCAACCGCCACATAGTC
ACATTGTCAAATAGCGTATTCACCTTCTCTTATAAGAAGGCTCAGCGAGATTTGGCGTAT
AAGCCACTCTACAGCTGGGAGGAAGCCAAGCAGAAAACGGTGGAGTGGGTTGGTTCCCTT
GTGGACCGGCACAAGGAGAACCTGAAGTCCAAGACTCAGTGA

Clone variation with respect to NM_000862.2
1012 c=>t;1100 c=>a
>OriGene 5' read for NM_000862 unedited
GGGCGGTCATAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCCCTC
TCCGGGTCACCCTAGNATCAGATCTGCTCCCCAGCATCTTCTGTTTCCTGGTGAGTGATT
CCTGCTACTTTGGATGGCCATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTT
TCTGGGACAGAGGATCATCCGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGT
CTTGGACAAGGCCTTCGGACCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGAC
CAAGCTGACAGTGCTGGAAGGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCA
GGACGTCTCGGTCATCATCCACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAG
AGAGTCTATCATGAATGTCAATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCA
AGCTAGTGTGCCAGTCTTCATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTA
CAAGGAAATCATCCAGAATGGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCC
ATACCCACACAGCAAAAAGCTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCT
GAAAAACGGCGGCACCCTGTACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGG
AAGCCGATTCCTTTCTGCTAGTATAAACGAGGCCCTGAACACAATGGGATCCTGTCAAGT
GTNGGAAAAGTCTCCACTGTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATT
CTGGCCTTGAGGNCCCCCTGCAGACCCCAGAAGGGCCCAGCATNNCGAGACAGTTCTACT
ATATCTCAGATGACACGCCTCACCAAAGCT
Restriction Sites NotI-NotI     
ACCN NM_000862
Insert Size 1750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000862.1, NP_000853.1
RefSeq Size 1665 bp
RefSeq ORF 1122 bp
Locus ID 3283
UniProt ID P14060
Domains 3Beta_HSD
Protein Families Transmembrane
Protein Pathways Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
Gene Summary The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...