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hCG (CGA) (NM_000735) Human Untagged Clone
CAT＃: SC119734
CGA (untagged)-Human glycoprotein hormones, alpha polypeptide (CGA)
CNY 1,200.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CG-ALPHA; FSHA; GPA1; GPHa; GPHA1; HCG; LHA; TSHA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119734 sequence for NM_000735 edited (data generated by NextGen Sequencing)
ATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATTGTCGGTGTTTCTGCAT GTTCTCCATTCCGCTCCTGATGTGCAGGATTGCCCAGAATGCACGCTACAGGAAAACCCA TTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCTGCTGCTTCTCTAGAGCA TATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAAAGAACGTCACCTCAGAG TCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTG GAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAA Clone variation with respect to NM_000735.2 >OriGene 5' read for NM_000735 unedited
GCTGGATTTAGGNAATCCGNATTTCACTATAGGGCGAGCCGCGCAATTCGGCACCAGNAC GAAGCTAAAATCCCTCTTCGGATCCACAGTCAACCGCCCTGAACACTCCTGCAAAAAGCC CAGAGAAAGGAGCGCCATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATT GTCGGTGTTTCTGCATGTTCTCCATTCCGCTCCTGTATGTTCAGGATTGCCCAGAATGCA CGCTACAGGAAAACCCATTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCT GCTGCTTCTCTAGAGCATATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAA AGAACGTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAA TGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC TTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA ATCAGCAGTCTAGTTCTTTTCATTTGGGATGAATACAGCATTTAGCTTGGTCCACTGCAA ATAAAGCCTTTTTAATCATCAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCNGCCGCG GTCATAGCTGTTTCCCTGACAGATCCCGGGTGGCATCCCTGTGACCCTCCCCAATGCCTT TCTGGGCCTGGAAGTGCCCCTCCAGTGCCAACAAACTTGGGCCTATAAATAAAGTGCATC ATTTTGNCTGACAAGGTGTCCCTCTTAAATTATGGGTTGAGAGGGTTGTGTTGAAAACCA GGGCAAATTTGGAAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000735 |
| Insert Size | 700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000735.2, NP_000726.1 |
| RefSeq Size | 704 bp |
| RefSeq ORF | 351 bp |
| Locus ID | 1081 |
| UniProt ID | P01215 |
| Domains | GHA |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Autoimmune thyroid disease, GnRH signaling pathway, Neuroactive ligand-receptor interaction |
| Gene Summary | The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Comparative Assessment on the Expression Level of Recombinant Human Follicle-Stimulating Hormone (FSH) in Serum-Containing Versus Protein-Free Culture Media
,Jazayeri, SH;Amiri-Yekta, A;Gourabi, H;Abd Emami, B;Halfinezhad, Z;Abolghasemi, S;Fatemi, N;Daneshipour, A;Ghahremani, MH;Sanati, MH;Khorramizadeh, MR;,
Mol. Biotechnol.
,PubMed ID 28993982
[CGA]
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Chromogranin A Induces the Biogenesis of Granules with Calcium- and Actin-Dependent Dynamics and Exocytosis in Constitutively Secreting Cells
,Salah Elias, Charlène Delestre, Stéphane Ory, Sébastien Marais, Maïté Courel, Rafael Vazquez-Martinez, Sophie Bernard, Laurent Coquet, Maria M. Malagon, Azeddine Driouich, Philippe Chan, Stéphane Gasman, Youssef Anouar, and Maité Montero-Hadjadje,
Endocrinology, Sep 2012; 153: 4444 - 4456.
[CGA]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203146 | CGA (Myc-DDK-tagged)-Human glycoprotein hormones, alpha polypeptide (CGA) |
CNY 1,200.00 |
|
| RC203146L1 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC203146L2 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), mGFP tagged |
CNY 5,890.00 |
|
| RC203146L3 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203146L4 | Lenti ORF clone of Human glycoprotein hormones, alpha polypeptide (CGA), mGFP tagged |
CNY 5,890.00 |
|
| RG203146 | CGA (tGFP-tagged) - Human glycoprotein hormones, alpha polypeptide (CGA) |
CNY 2,800.00 |
