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5 HT 2A (HTR2A) (NM_000621) Human Untagged Clone
CAT＃: SC119793
HTR2A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1
CNY 5,488.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5-HT2A; HTR2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119793 sequence for NM_000621 edited (data generated by NextGen Sequencing)
ATGGATATTCTTTGTGAAGAAAATACTTCTTTGAGCTCAACTACGAACTCCCTAATGCAATTAAATGATG ACAACAGGCTCTACAGTAATGACTTTAACTCCGGAGAAGCTAACACTTCTGATGCATTTAACTGGACAGT CGACTCTGAAAATCGAACCAACCTTTCCTGTGAAGGGTGCCTCTCACCGTCGTGTCTCTCCTTACTTCAT CTCCAGGAAAAAAACTGGTCTGCTTTACTGACAGCCGTAGTGATTATTCTAACTATTGCTGGAAACATAC TCGTCATCATGGCAGTGTCCCTAGAGAAAAAGCTGCAGAATGCCACCAACTATTTCCTGATGTCACTTGC CATAGCTGATATGCTGCTGGGTTTCCTTGTCATGCCCGTGTCCATGTTAACCATCCTGTATGGGTACCGG TGGCCTCTGCCGAGCAAGCTTTGTGCAGTCTGGATTTACCTGGACGTGCTCTTCTCCACGGCCTCCATCA TGCACCTCTGCGCCATCTCGCTGGACCGCTACGTCGCCATCCAGAATCCCATCCACCACAGCCGCTTCAA CTCCAGAACTAAGGCATTTCTGAAAATCATTGCTGTTTGGACCATATCAGTAGGTATATCCATGCCAATA CCAGTCTTTGGGCTACAGGACGATTCGAAGGTCTTTAAGGAGGGGAGTTGCTTACTCGCCGATGATAACT TTGTCCTGATCGGCTCTTTTGTGTCATTTTTCATTCCCTTAACCATCATGGTGATCACCTACTTTCTAAC TATCAAGTCACTCCAGAAAGAAGCTACTTTGTGTGTAAGTGATCTTGGCACACGGGCCAAATTAGCTTCT TTCAGCTTCCTCCCTCAGAGTTCTTTGTCTTCAGAAAAGCTCTTCCAGCGGTCGATCCATAGGGAGCCAG GGTCCTACACAGGCAGGAGGACTATGCAGTCCATCAGCAATGAGCAAAAGGCATGCAAGGTGCTGGGCAT CGTCTTCTTCCTGTTTGTGGTGATGTGGTGCCCTTTCTTCATCACAAACATCATGGCCGTCATCTGCAAA GAGTCCTGCAATGAGGATGTCATTGGGGCCCTGCTCAATGTGTTTGTTTGGATCGGTTATCTCTCTTCAG CAGTCAACCCACTAGTCTACACACTGTTCAACAAGACCTATAGGTCAGCCTTTTCACGGTATATTCAGTG TCAGTACAAGGAAAACAAAAAACCATTGCAGTTAATTTTAGTGAACACAATACCGGCTTTGGCCTACAAG TCTAGCCAACTTCAAATGGGACAAAAAAAGAATTCAAAGCAAGATGCCAAGACAACAGATAATGACTGCT CAATGGTTGCTCTAGGAAAGCAGCATTCTGAAGAGGCTTCTAAAGACAATAGCGACGGAGTGAATGAAAA GGTGAGCTGTGTGTGA Clone variation with respect to NM_000621.4 74:a=>c >OriGene 5' read for NM_000621 unedited
GCANAANAGGGGACTCTACACCAGTTTAATTACTGTGAGAGATGCAGCGAGTCACAGAAT AACAAATGTATCTCATGTGTGGACCCTGAAGACAAATGACATTTATCTTCCCGAGCGCTC AAAAAAAACCCTGCAACCTCTATGCTAAAAGTTCATTCTGCTTTTTTGTCCTCGGTTTTT TTTATTAAAATAATAAAACCAAACAGTGGACTCTCCTAAAATTGTGAATGAAGAAAACTT ACAGCCACCACAGTTCAGTTCTTTAACTATCATTGTAATAATGGAAGACAAAAATCCAGC CCCGGGAGAACAGCATGTACACCAGCCTCAGTGTTACAGAGTGTGGGTACATCAAGGTGA ATGGTGAGCAGAAACTATAACCTGTTAGTCCTTCTACACCTCATCTGCTACAAGTTCTGG CTTAGACATGGATATTCTTTGTGAAGAAAATACTTCTTTGAGCTCAACTACGAACTCCCT AATGCAATTAAATGATGACAACAGGCTCTACAGTAATGACTTTAACTCCGGAGAAGCTAA CACTTCTGATGCATTTAACTGGACAGTCGACTCTGAAAATCGAACCAACCTTTCCTGTGA AGGGTGCCTCTCACCGTCGTGTCTCTCCTTACTTCATCTCCAGGAAAAAAACTGGTCTGC TTTACTGACAGCCGTAGTGATTATTCTAACTATTGCTGNGAACATACTCGTCATCATGGC AGTGTCCCTAGAGAAAAAGCTGCAGAATGCCACCAACTATTTCCTGATGTCACTTGCCAT AGCTGATATGCTGCTGGGTTTCCTTGTCATGCCCGTGTCCATGTTAACCATCCTGTATGG GTACCCGTGGCCTCTGCCGAGCAGCTTTGTGCAGTCTGGATT >OriGene 3' read for NM_000621 unedited
GCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTGATAGGGTCTCACTCTG TTGCCCAGGAGGAAATGGCAGTGGTGTGAACTTGGCTCACTGCAGCCTTGAGCTTCTGGG CTCAAGTGATCCTTGCGTCTCAGCCTCCCCTGTAGCTGGGACCACAGTTGTGTGCCACAA GGATCAGATAATTTTTTGATTTTTTTTGTAGAGACAGGGAGGTCTCACTTTGTTACCCAG GTTGGTCTCAAACTCTTGGCCTCAAGTGATCCTCCCATCTCAGCCTCCCAAAGTGCTAGG ATTACATGCACGAGCCTCCGTGCCTGGCCGAGCCATTTCGTTTGAAGGCAAGGATTTTGT TTTAATTATTTTTATATTTCTCATATCTAGAAATTTGCTTCACACATAAATGTACACTCA ATAGATGATTGTGGAACCAAGCAGAATGGTTTTAATAAATAGTATAAACCGATGGATCTG AAAGCATCAGTTTACCTGATGAGTGTCATAGGAAAGTCACCTGCTTTTTACAGAGATGTC CTAAGACTCTACCACTGTCAGTAGCCGGAAACCTGAGTTCATCCTCAATCCTGACACAGT TACATCAATATACATTTACTGCTCAAAAGAGTGCATGCATGATGCAGTCATTTCACAGCA AGTTACCAAATACCTCGATAGTGCTGTTTTAGAAGAGCAATTGCTAGAAAAGCATTTGGT AATGGTAAATCATCCTTTTGCTAGTTACCTAGAGGAACAATATTAAACNAGACACACTGG AATTCAGAGTAAGCCCAGAAGTGAAGAGAAGCAGCATAGCTATGGATACATTTGAGTCTT TTCACTCTGAGATTACTTGAATGCCCAATGCCACACCCTGGATATTTCAAATGTTCTATG AAAACAGATGAAAATGGTGCAGTTTTATTAATATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000621 |
| Insert Size | 3300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_000621.2. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000621.2, NP_000612.1 |
| RefSeq Size | 5442 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 3356 |
| UniProt ID | P28223 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction |
| Gene Summary | This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Antidepressant-Like Effects of Vaccinium bracteatum in Chronic Restraint Stress Mice: Functional Actions and Mechanism Explorations
,Oh, DR;Kim, Y;Choi, EJ;Jung, MA;Oh, KN;Hong, JA;Bae, D;Kim, K;Kang, H;Kim, J;Kim, YR;Cho, SS;Choi, CY;,
Am. J. Chin. Med.
,PubMed ID 29444592
[HTR2A]
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Molecular pharmacology and ligand docking studies reveal a single amino acid difference between mouse and human serotonin 5-HT2A receptors that impacts behavioral translation of novel 4-phenyl-2-dimethylaminotetralin ligands
,Canal, CE;Cordova-Sintjago, T;Liu, Y;Kim, MS;Morgan, D;Booth, RG;,
J Pharmacol Exp Ther. 2013 Dec;347(3):705-16
,PubMed ID 24080681
[HTR2A]
|
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c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta
,Rong Lu, Abderrahmane Alioua, Yogesh Kumar, Pallob Kundu, Mansoureh Eghbali, Noelia V. Weisstaub, Jay A. Gingrich, Enrico Stefani, and Ligia Toro,
J. Physiol. 2008; 586(16): p. 3855-3869
[HTR2A]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210182 | HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 5,488.00 |
|
| RC210182L1 | Lenti-ORF clone of HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RC210182L2 | Lenti-ORF clone of HTR2A (mGFP-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RC210182L3 | Lenti-ORF clone of HTR2A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 5,890.00 |
|
| RC210182L4 | Lenti-ORF clone of HTR2A (mGFP-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,888.00 |
|
| RG210182 | HTR2A (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1 |
CNY 7,088.00 |
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