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Hamartin (TSC1) (NM_000368) Human Untagged Clone
CAT＃: SC119935
TSC1 (untagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 1
CNY 8,112.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LAM; TSC |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000368 edited
GAATTCGGCACGAGGGCGCCCTGTAGGCTGGAGGGACTGTGAGTGACCATGAAAGACACC AGGTTGACAGCACTGGAAACTGAAGTACCAGTTGTCGCTAGAACAGTTTGGTAGTGGCCC CAATGAAGAACCTTCAGAACCTGTAGCACACGTCCTGGAGCCAGCACAGCGCCTTCGAGC GAGAGAATGGCCCAACAAGCAAATGTCGGGGAGCTTCTTGCCATGCTGGACTCCCCCATG CTGGGTGTGCGGGACGACGTGACAGCTGTCTTTAAAGAGAACCTCAATTCTGACCGTGGC CCTATGCTTGTAAACACCTTGGTGGATTATTACCTGGAAACCAGCTCTCAGCCGGCATTG CACATCCTGACCACCTTGCAAGAGCCACATGACAAGCACCTCTTGGACAGGATTAACGAA TATGTGGGCAAAGCCGCCACTCGTTTATCCATCCTCTCGTTACTGGGTCATGTCATAAGA CTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTTTGCCTTCTTTACTAAAA TGTCTCAAGATGGACACTGACGTCGTTGTCCTCACAACAGGCGTCTTGGTGTTGATAACC ATGCTACCAATGATTCCACAGTCTGGGAAACAGCATCTTCTTGATTTCTTTGACATTTTT GGCCGTCTGTCATCATGGTGCCTGAAGAAACCAGGCCACGTGGCGGAAGTCTATCTCGTC CATCTCCATGCCAGTGTGTACGCACTCTTTCATCGCCTTTATGGAATGTACCCTTGCAAC TTCGTCTCCTTTTTGCGTTCTCATTACAGTATGAAAGAAAACCTGGAGACTTTTGAAGAA GTGGTCAAGCCAATGATGGAGCATGTGCGAATTCATCCGGAATTAGTGACTGGATCCAAG GACCATGAACTGGACCCTCGAAGGTGGAAGAGATTAGAAACTCATGATGTTGTGATCGAG TGTGCCAAAATCTCTCTGGATCCCACAGAAGCCTCATATGAAGATGGCTATTCTGTGTCT CACCAAATCTCAGCCCGCTTTCCTCATCGTTCAGCCGATGTCACCACCAGCCCTTATGCT GACACACAGAATAGCTATGGGTGTGCTACTTCTACCCCTTACTCCACGTCTCGGCTGATG TTGTTAAATATGCCAGGGCAGCTACCTCAGACTCTGAGTTCCCCATCGACACGGCTGATA ACTGAACCACCACAAGCTACTCTTTGGAGCCCATCTATGGTTTGTGGTATGACCACTCCT CCAACTTCTCCTGGAAATGTCCCACCTGATCTGTCACACCCTTACAGTAAAGTCTTTGGT ACAACTGCAGGTGGAAAAGGAACTCCTCTGGGAACCCCAGCAACCTCTCCTCCTCCAGCC CCACTCTGTCATTCGGATGACTACGTGCACATTTCACTCCCCCAGGCCACAGTCACACCC CCCAGGAAGGAAGAGAGAATGGATTCTGCAAGACCATGTCTACACAGACAACACCATCTT CTGAATGACAGAGGATCAGAAGAGCCACCTGGCAGCAAAGGTTCTGTCACTCTAAGTGAT CTTCCAGGGTTTTTAGGTGATCTGGCCTCTGAAGAAGATAGTATTGAAAAAGATAAAGAA GAAGCTGCAATATCTAGAGAACTTTCTGAGATCACCACAGCAGAGGCAGAGCCTGTGGTT CCTCGAGGAGGCTTTGACTCTCCCTTTTACCGAGACAGTCTCCCAGGTTCTCAGCGGAAG ACCCACTCGGCAGCCTCCAGTTCTCAGGGCGCCAGCGTGAACCCTGAGCCTTTACACTCC TCCCTGGACAAGCTTGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCTGCCC TGCGGCAGTGCTGATGAAAGCCCTGCGGGAGACAGGGAATGCCAGACTTCTTTGGAGACC AGTATCTTCACTCCCAGTCCTTGTAAAATTCCACCTCCGACGAGAGTGGGCTTTGGAAGC GGGCAGCCTCCCCCGTATGATCATCTTTTTGAGGTGGCATTGCCAAAGACAGCCCATCAT TTTGTCATCAGGAAGACTGAGGAGCTGTTAAAGAAAGCAAAAGGAAACACAGAGGAAGAT GGTGTGCCCTCTACCTCCCCAATGGAAGTGCTGGACAGACTGATACAGCAGGGAGCAGAC GCGCACAGCAAGGAGCTGAACAAGTTGCCTTTACCCAGCAAGTCTGTCGACTGGACCCAC TTTGGAGGCTCTCCTCCTTCAGATGAGATCCGCACCCTCCGAGACCAGTTGCTTTTACTG CACAACCAGTTACTCTATGAGCGTTTTAAGAGGCAGCAGCATGCCCTCCGGAACAGGCGG CTCCTCCGCAAGGTGATCAAAGCAGCAGCTCTGGAGGAACATAATGCTGCCATGAAAGAT CAGTTGAAGTTACAAGAGAAGGACATCCAGATGTGGAAGGTTAGTCTGCAGAAAGAACAA GCTAGATACAATCAGCTCCAGGAGCAGCGTGACACTATGGTAACCAAGCTCCACAGCCAG ATCAGACAGCTGCAGCATGACCGAGAGGAATTCTACAACCAGAGCCAGGAATTACAGACG AAGCTGGAGGACTGCAGGAACATGATTGCGGAGCTGCGGATAGAACTGAAGAAGGCCAAC AACAAGGTGTGTCACACTGAGCTGCTGCTCAGTCAGGTTTCCCAAAAGCTCTCAAACAGT GAGTCGGTCCAGCAGCAGATGGAGTTCTTGAACAGGCAGCTGTTGGTTCTTGGGGAGGTC AACGAGCTCTATTTGGAACAACTGCAGAACAAGCACTCAGATACCACAAAGGAAGTAGAA ATGATGAAAGCCGCCTATCGGAAAGAGCTAGAAAAAAACAGAAGCCATGTTCTCCAGCAG ACTCAGAGGCTTGATACCTCCCAAAAACGGATTTTGGAACTGGAATCTCACCTGGCCAAG AAAGACCACCTTCTTTTGGAACAGAAGAAATATCTAGAGGATGTCAAACTCCAGGCAAGA GGACAGCTGCAGGCCGCAGAGAGCAGGTATGAGGCTCAGAAAAGGATAACCCAGGTGTTT GAATTGGAGATCTTAGATTTATATGGCAGGTTGGAGAAAGATGGCCTCCTGAAAAAACTT GAAGAAGAAAAAGCAGAAGCAGCTGAAGCAGCAGAAGAAAGGCTTTCTTACAGGCTTGAC TGTTGTAATGACGGGTGCTCAGATTCCATGGTAGGGCACAATGAAGAGGCATCTGGCCAC AACGGTGAGACCAAGACCCCCAGGCCCAGCAGCGCCCGGGGCAGTAGTGGAAGCAGAGGT GGTGGAGGCAGCAGCAGCAGCAGCAGCGAGCTTTCTACCCCAGAGAAACCCCCACACCAG AGGGCAGGCCCATTCAGCAGTCGGTGGGAGACGACTATGGGAGAAGCGTCTGCCAGCATC CCCACCACTGTGGGCTCACTTCCCAGTTCAAAAAGCTTCCTGGGTATGAAGGCTCGAGAG TTATTTCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACGGCATGACCAGTAGCCTTTCT GAGAGCCTAAAGACAGAACTGGGCAAAGACTTGGGTGTGGAAGCCAAGATTCCCCTGAAC CTAGATGGCCCTCACCCGTCTCCCCCGACCCCGGACAGTGTTGGACAGCTACATATCATG GACTACAATGAGACTCATCATGAACACAGCTAAGGAATGATGGTCAATCAGTGTTAACTT GCATATTGTTGGCACAGAACAGGAGGTGTGAATGCACGTTTCAAAGCTTTCCTGTTTCCA GGGTCTGAGTGCAAGTTCATGTGTGGAAATGGGACGGAGGTCCTTTGGACAGCTGACTGA ATGCAGAACGGTTTTTGGATCTGGCATTGAAATGCCTCTTGACCTTCCCCTCCACCCGCC CTAACCCCCTCTCATTTACCTCGCAGTGTGTTCTAATCCAAGGGCCAGTTGGTGTTCCTC AGTAGCTTTACTTTCTTCCTTCCCCCCCAAATGGTTGCGTCCTTTGAACCTGTGCAATAT GAGGCCAAATTTAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_000368 unedited
AAGTTTAGATTTGTATACGACTTATATAGGCGGCCGACGAATTCGCACGAGGGCGCCCTG TAGGCTGGAGGGACTGTGAGTGACCATAGAAAGACACCAGGTTGACAGCACTGGAAACTG AAGTACCAGTTGTCGCTAGAACAGTTTGGTAGTGGCCCCAATGAAGAACCTTCAGAACCT GTAGCACACGTCCTGGAGCCAGCACAGCGCCTTCGAGCGAGAGAATGGCCCAACAAGCAA ATGTCGGGGAGCTTCTTGCCATGCTGGACTCCCCCATGCTGGGTGTGCGGGACGACGTGA CAGCTGTCTTTAAAGAGAACCTCAATTCTGACCGTGGCCCTATGCTTGTAAACACCTTGG TGGATTATTACCTGGAAACCAGCTCTCAGCCGGCATTGCACATCCTGACCACCTTGCAAG AGCCACATGACAAGCACCTCTTGGACAGGATTAACGAATATGTGGGCAAAGCCGCCACTC GTTTATCCATCCTCTCGTTACTGGGTCATGTCATAAGACTGCAGCCATCTTGGAAGCATA AGCTCTCTCAAGCACCTCTTTTGCCTTCTTTACTAAAATGTCTCAAGATGGACACTGACG TCGTTGTCCTCACAACAGGCGTCTTGGTGTTGATAACCATGCTACCAATGATTCCACAGT CTGGGAAACAGCATCTTCTTGATTTCTTTGACATTTTTAGCCGTCTGTCATCATGGTGCC TGAAGAAACCAGGCCACGTGGCANAAGTCTATCTCGTCCATCTCCATGCCAGTGTGTACG CACTCTTTCATCGCCTTTATGGAATGTACCCTTAGCACTTCGTCTCCTTTTTGCGTTCTC ATTACAGTATGAAAGAAAACCTGNAGACTTTTGNAGAAGTGGGTCAGCCCATGATGGAGC ATGTGCGAATTNCTCCCGGATTAGTGACT >OriGene 3' read for NM_000368 unedited
ATTAGCTNTGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTAAATT TGGCCTCATATTGCACAGGTTCAAAGGACGCAACCATTTGGGGGGGAAGGAAGAAAGTAA AGCTACTGAGGAACACCAACTGGCCCTTGGATTAGAACACACTGCGAGGTAAATGAGAGG GGGTTAGGGCGGGTGGAGGGGAAGGTCAAGAGGCATTTCAATGCCAGATCCAAAAACCGT TCTGCATTCAGTCAGCTGTCCAAAGGACCTCCGTCCCATTTCCACACATGAACTTGCACT CAGACCCTGGAAACAGGAAAGCTTTGAAACGTGCATTCACACCTCCTGTTCTGTGCCAAC AATATGCAAGTTAACACTGATTGACCATCATTCCTTAGCTGTGTTCATGATGAGTCTCAT TGTAGTCCATGATATGTAGCTGTCCAACACTGTCCGGGGTCGGGGGAGACGGGTGAGGGC CATCTAGGTTCAGGGGAATCTTGGCTTCCACACCCAAGTCTTTGCCCAGTTCTGTCTTTA GGCTCTCAGAAAGGCTACTGGTCATGCCGTCCTCATCACACTGGCTCTCGCTCTTATTAC GAAATAACTCTCGAGCCTTCATACCCAGGAAGCTTTTTGAACTGTGAAGTGAGCCCACAG TGGTGGCGATGCTGTCAGACGCTTCTCCCATAGTCCGTTCCCACCGACTGCTGAATGGGC CTGCCCTCTTGTGTGGGGGGTTTCTCTGGGTACCAAGCTCGCTGCTGTCGTTGCTGATGC CTCCACACCTCTGTTTCCCTATTGCCCCGGAGCTGTTGGACCCGGGGGTCTGGCCTTACC GTTGTGGCCAGATGCTTCTCATGNGCCCTACATGGAATCTCGCCCCCTGTATTCCAACCT CAAGCTGCTCAACCCCCTCTCCCGTGCTTCATTCGCCTTGCCCTCCCTTTCCAATTCTTC CG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000368 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000368.3, NP_000359.1 |
| RefSeq Size | 8617 bp |
| RefSeq ORF | 3495 bp |
| Locus ID | 7248 |
| UniProt ID | Q92574 |
| Domains | Hamartin |
| Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
| Gene Summary | This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213332 | TSC1 (Myc-DDK-tagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 1 |
CNY 12,200.00 |
|
| RC213332L1 | Lenti ORF clone of Human tuberous sclerosis 1 (TSC1), transcript variant 1, Myc-DDK-tagged |
CNY 14,600.00 |
|
| RC213332L2 | Lenti ORF clone of Human tuberous sclerosis 1 (TSC1), transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
| RC213332L3 | Lenti ORF clone of Human tuberous sclerosis 1 (TSC1), transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC213332L4 | Lenti ORF clone of Human tuberous sclerosis 1 (TSC1), transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
| RG213332 | TSC1 (tGFP-tagged) - Human tuberous sclerosis 1 (TSC1), transcript variant 1 |
CNY 13,800.00 |
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