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PPT1 (NM_000310) Human Untagged Clone
CAT＃: SC119961
PPT1 (untagged)-Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLN1; INCL; PPT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119961 sequence for NM_000310 edited (data generated by NextGen Sequencing)
ATGGCGTCGCCCGGCTGCCTGTGGCTCTTGGCTGTGGCTCTCCTGCCATGGACCTGCGCT TCTCGGGCGCTGCAGCATCTGGACCCGCCGGCGCCGCTGCCGTTGGTGATCTGGCATGGG ATGGGAGACAGCTGTTGCAATCCCTTAAGCATGGGTGCTATTAAAAAAATGGTGGAGAAG AAAATACCTGGAATTTACGTCTTATCTTTAGAGATTGGGAAGACCCTGATGGAGGACGTG GAGAACAGCTTCTTCTTGAATGTCAATTCCCAAGTAACAACAGTGTGTCAGGCACTTGCT AAGGATCCTAAATTGCAGCAAGGCTACAATGCTATGGGATTCTCCCAGGGAGGCCAATTT CTGAGGGCAGTGGCTCAGAGATGCCCTTCACCTCCCATGATCAATCTGATCTCGGTTGGG GGACAACATCAAGGTGTTTTTGGACTCCCTCGATGCCCAGGAGAGAGCTCTCACATCTGT GACTTCATCCGAAAAACACTGAATGCTGGGGCGTACTCCAAAGTTGTTCAGGAACGCCTC GTGCAAGCCGAATACTGGCATGACCCCATAAAGGAGGATGTGTATCGCAACCACAGCATC TTCTTGGCAGATATAAATCAGGAGCGGGGTATCAATGAGTCCTACAAGAAAAACCTGATG GCCCTGAAGAAGTTTGTGATGGTGAAATTCCTCAATGATTCCATTGTGGACCCTGTAGAT TCGGAGTGGTTTGGATTTTACAGAAGTGGCCAAGCCAAGGAAACCATTCCCTTACAGGAG ACCTCCCTGTACACACAGGACCGCCTGGGGCTAAAGGAAATGGACAATGCAGGACAGCTA GTGTTTCTGGCTACAGAAGGGGACCATCTTCAGTTGTCTGAAGAATGGTTTTATGCCCAC ATCATACCATTCCTTGGATGA Clone variation with respect to NM_000310.3 >OriGene 5' read for NM_000310 unedited
GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACACAGCGAAGATGGCG TCGCCCGGCTGCCTGTGGCTCTTGGCTGTGGCTCTCCTGCCATGGACCTGCGCTTCTCGG GCGCTGCAGCATCTGGACCCGCCGGCGCCGCTGCCGTTGGTGATCTGGCATGGGATGGGA GACAGCTGTTGCAATCCCTTAAGCATGGGTGCTATTAAAAAAATGGTGGAGAAGAAAATA CCTGGAATTTACGTCTTATCTTTAGAGATTGGGAAGACCCTGATGGAGGACGTGGAGAAC AGCTTCTTCTTGAATGTCAATTCCCAAGTAACAACAGTGTGTCAGGCACTTGCTAAGGAT CCTAAATTGCAGCAAGGCTACAATGCTATGGGATTCTCCCAGGGAGGCCAATTTCTGAGG GCAGTGGCTCAGAGATGCCCTTCACCTCCCATGATCAATCTGATCTCGGTTGGGGGACAA CATCAAGGTGTTTTTGGACTCCCTCGATGCCCAGGAGAGAGCTCTCACATCTGTGACTTC ATCCGAAAAACACTGAATGCTGGGGCGTACTCCAAAGTTGTTCAGGAACGCCTCGTGCAA GCCGAATACTGGCATGACCCCATAAAGGAGGATGTGTATCGCAACCACAGCATCTTCTTG GCAGATATAAATCAGGAGCGGNGTATCAATGAGTCCTACAGNAAAACCTGATGGCCCTGA AGAAGTTTGTGATGGTGAAATTCCTCAATGATTCCATTGTGGNACCCTGTAGATTCNGNA GTGGTTGGNNATTTACAGAAAGTGGCCAAGCCAAGGAAACCATTCCCTTACAGAGAACCT CCTGTCACACAGNACCGNNCTGGGCTAAAGGAATGGACATGCAGACAGCTAGTGTTTCTG CTCAGAGGNGACCATCTCAGTGCTGAAA >OriGene 3' read for NM_000310 unedited
ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAAACTACTCTTTA TTTTAGACAACCTCATAAAATTATTTTCACATCCCCCCCAACTTCTTGCTCTTAATCCTC ATCTTTTAGTTGAAGAATAAGGCTTAAGAGAGAGAAAGGAAAAACCATAATGGCTAAACT TAGCAGCACCAACACGGTTCTTTCATCAAGGCGTAGCTCATCATTTCTCCAAACTGACAT GCTACAGAAATGTCTTCCAAAAAGCGTTAAGTTTTCACAGAGTGGGGACTATGATTTCCA TGCTCAAATAGTGTAGGAAATGGTAGATTACGTTAGTTTTGTTTACTGTAACAGGAATTC TCAGGAGTCCTTAGACACTCTAATGTGGGTTGATCTCCAGAAGGAGAAAGGGAGTGTGTA GTATTTTCCAAGCTTTTTGAGCAAACAATCCTTTTCTCATGGAACACATCAGAAACAGTT TGGAGTATGCTGGTAAATGTGTTTTGGGGATGGAAGTCAGAAAGCCTGATCTTTTTCATA TGGTTCCTCCTATTCTCTGCTAAAGCCAGCAACAGAGTAAGTTGACTCTAGCATCAAGAC CACTAGTCATATTAAGAAAGGGCAGGTTGGTCGAGAATGGAATAGGAAGAACTAGCTGCC TCACTTTTCACTTTCGCGCCTACATTTTCTTCGCTCCCTAAGTTATACAAGATGTACATT TCCTCTCTTAACAATTTATTTGGGTGCTACTTCCCCTCCTTTGTGACGCAACTATTTATC ACTACCTGAGGAGACAAAGTGGGTGCTGCATAAGCAAGACTTGTAGCCAGGGGGATTGCC TATAATTGAGATAAGAGATTGATGCCCCNAGGGCACGTCTCAAAAGGAAGCGCGCTTAAA AAGATGCGAATACCAGCAGATTTTCAGCACTGTTAATACACCAACAAGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000310 |
| Insert Size | 2610 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000310.2, NP_000301.1 |
| RefSeq Size | 2358 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 5538 |
| UniProt ID | P50897 |
| Domains | Palm_thioest |
| Protein Families | Druggable Genome |
| Protein Pathways | Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Role of Ceroid Lipofuscinosis Neuronal Protein 5 (CLN5) in Endosomal Sorting
,Aline Mamo, Felix Jules, Karine Dumaresq-Doiron, Santiago Costantino, and Stephane Lefrancois,
Mol. Cell. Biol., May 2012; 32: 1855 - 1866.
[PPT1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203278 | PPT1 (Myc-DDK-tagged)-Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1 |
CNY 2,400.00 |
|
| RC203278L1 | Lenti ORF clone of Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203278L2 | Lenti ORF clone of Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203278L3 | Lenti ORF clone of Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203278L4 | Lenti ORF clone of Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203278 | PPT1 (tGFP-tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1 |
CNY 4,000.00 |
