Signal peptide peptidase like 2B (SPPL2B) (NM_020172) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IMP4, PSL1, KIAA1532; RGD1308556; signal peptide peptidase-like 2B
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC120624 sequence for NM_020172 edited (data generated by NextGen Sequencing) ATGGTGCACGTGGTCTCCCAGGCCGGGGGCCCCGAAGGCAAAGACTACTGCATCCTCTAC AACCCGCAGTGGGCCCATCTTCCGCACGACCTCAGCAAGGCATCTTTCCTGCAGCTGCGC AACTGGACGGCCTCCCTGCTCTGCTCCGCAGCCGACCTCCCCGCCCGTGGCTTCAGCAAC CAGATCCCGCTGGTGGCGCGGGGGAACTGCACCTTCTATGAGAAAGTGAGGCTGGCCCAG GGCAGCGGAGCACGCGGGCTGCTCATCGTCAGCAGGGAGAGGCTGGTCCCCCCGGGGGGT AATAAGACGCAGTATGATGAGATTGGCATTCCCGTGGCCCTGCTCAGCTACAAAGACATG CTGGACATCTTCACGCGTTTCGGCCGCACGGTGAGGGCGGCGCTGTATGCGCCTAAGGAG CCGGTGCTGGACTACAACATGGTCATCATCTTCATCATGGCTGTGGGCACCGTCGCCATC GGCGGCTACTGGGCCGGGAGTCGGGACGTGAAGAAAAGGTACATGAAGCACAAGCGCGAC GATGGGCCCGAGAAGCAGGAGGACGAGGCGGTGGACGTGACGCCGGTGATGACCTGCGTG TTTGTGGTGATGTGCTGCTCCATGCTGGTGCTGCTCTACTATTTCTACGACCTCCTCGTG TACGTGGTCATCGGGATCTTCTGCCTGGCCTCCGCCACCGGCCTCTACAGCTGCCTGGCG CCCTGTGTGCGGCGGCTGCCCTTCGGCAAGTGCAGGATCCCCAACAACAGCCTGCCCTAC TTCCACAAGCGCCCGCAGGCCCGTATGCTGCTCCTGGCGCTCTTCTGCGTGGCCGTCAGC GTGGTGTGGGGCGTCTTCCGCAACGAGGACCAGTGGGCCTGGGTCCTCCAGGATGCCCTG GGCATCGCCTTCTGCCTCTACATGCTGAAGACCATCCGTCTGCCCACCTTCAAGGCCTGC ACGCTGCTGCTGCTGGTGCTGTTCCTCTACGACATCTTCTTCGTGTTCATCACGCCCTTC CTGACCAAGAGTGGGAGCAGCATCATGGTGGAGGTGGCCACTGGGCCCTCGGACTCAGCC ACCCGTGAGAAGCTGCCCATGGTCCTGAAGGTGCCCAGGCTGAACTCCTCACCTCTGGCC CTGTGTGACCGGCCCTTCTCCCTCCTGGGTTTCGGAGACATTTTGGTGCCAGGGCTGCTG GTGGCCTACTGCCACAGGTTTGACATCCAGGTACAGTCCTCCAGGGTATACTTCGTGGCC TGCACCATCGCCTATGGCGTTGGCCTCCTTGTGACATTCGTGGCACTGGCCCTGATGCAG CGTGGCCAGCCCGCTCTCCTCTACCTGGTGCCCTGCACGCTGGTGACGAGCTGCGCTGTG GCGCTCTGGCGCCGGGAGCTGGGCGTGTTCTGGACGGGCAGCGGCTTTGCGAAAGTCCTA CCTCCATCTCCGTGGGCCCCAGCACCAGCCGACGGCCCGCAGCCTCCCAAAGACTCTGCC ACGCCACTCTCCCCGCAGCCGCCCAGCGAAGAACCAGCCACATCCCCCTGGCCTGCTGAG CAGTCCCCAAAATCACGCACGTCCGAGGAGATGGGGGCTGGAGCCCCCATGCGGGAGCCT GGGAGCCCAGCTGAATCCGAGGGCCGGGACCAGGCCCAGCCGTCCCCGGTAACCCAGCCT GGCGCCTCGGCCTAG Clone variation with respect to NM_020172.1 651 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_020172 unedited TCAACATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAAACCGGCCGA CATGGCGGCAGCGGTGGCGGCTGCGCTGGCGCGGCTTTTGGCGGCCTTTCTGCTCCTCGC GGCCCAGGTGGCCTGTGAGTACGGCATGGTGCACGTGGTCTCCCAGGCCGGGGGCCCCGA AGGCAAAGACTACTGCATCCTCTACAACCCGCAGTGGGCCCATCTTCCGCACGACCTCAG CAAGGCATCTTTCCTGCAGCTGCGCAACTGGACAGCCTCCCTGCTCTGCTCCGCAGCCGA CCTCCCCGCCCGTGGCTTCAGCAACCAGATCCCGCTGGTGGCGCGGGGGAACTGCACCTT CTATGAGAAAGTGAGGCTGGCCCAGGGCAGCGGAGCACGCGGGCTGCTCATCGTCAGCAG GGAGAGGCTGGTCCCCCCGGGGGGTAATAAGACGCAGTATGATGAGATTGGCATTCCCGT GGCCCTGCTCAGCTACAAAGACATGCTGGACATCTTCACGCGTTTCGGCCGCACGGTGAG GGCGGCGCTGTATGCGCCTAAGGAGCCGGTGCTGGACTACAACATGGTCATCATCTTCAT CATGGCTGTGGGCACCGTCGCCATCGGCGGCTACTGGGCCGGGAGTCGGGACGTGAAGAA AAGGTACATGAAGCACAAGCGCGACGATGGGCCCGAGAAGCANGANGACGAGGCGGTGGA CGTGACGCCGGTGATGACCTGCGTGTTTGTGGTGATGTGCTGCTCCATGCTGGTGCTGCT CTACTATTTCTACGACCTCCTCGTGTACGTGGTCATCGGGATCTTCTGCCTGGCCTCCGC CACCGGCCTCTACAGCTGCCTGGCGCCCTGTGTGCGGCGGCTGCCCTTCGGCAGTGCAAG ATCCCCAACACAGCCTGGCCTACTTCACAAGCGCCCGCAGCCCGTTGCTGCTCTGGCGCT N 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_020172 unedited NCGGCCTCTATGTACCGCGCCGCATTCTAGACTCGGTTTTTTTTTTTTTTTTTTTCTAGA ATTGCGACCATTTTTAAAAGGGGAGGAGTGTGCAGGCATGGCCAGGCACCTCTGGCTGAG GGCAGGGCAGGGCCTTAGCCAGGGGAGCAGCTTGTCCACTTACTGCCAGCCTTGTTCACG CCCCAAAACCCCGCCCCACGGGCCTGTCTCAGCCAGCGTCAGGGGCCAGAGGCCGCCCCG GCCACCGGTCCGTGTCCTAGGCTTGGAGGCAGCTTCCAAACCTCCCAGGGGCTCCGATCT GCTCAAGAGGGAAAAGCGATGCCTTCTGGGAGACAGAAAGCTGCTCTTCCACCCGGAGAG CAGGATGCCGTGGGGAGGGAGGTCACCTGAAGGCCGAAGCAGCTGGTGAGCACCAAGTGT GGCCGCGGCCAGAGTCCACCCACGCCTGCTCACTCATGCAGACGACGCTTGAGGGTCTGC AGAGAGGCCGGGCAGGGCCTGCGAGGACGGACCCCGGTGGGGAGGATGGACCCGCAGAGA GCTTGTGGCAGCCGGGCGAGCTGGGAGCAGCAGGCGTGCAGCCGGGGCAGCTGGGCTGGG CGCGGGCACAGACCGCAGGGGTCTCGGCAAGGATGATCACCATGTTGGGGCGGGTGGGGA CGGCACAGGCCTCGGTCCCGGGGGACAGGCGTCTGTCTGTCTGTCTCCAGTGGGCGCCAG GCAGCCCCAACATCTTGGTGTGGCGGGCACGGCAGCGAGCGTCTCACCCCTCCCCTAGGC CGATGCGCCAGGCTGGGTTACCGGGGACGGCTGGGCCTGGTCCCGGCCCTCTGATTCAGC TGGGCTCCAAGGCTCCCGAATGGG
Restriction Sites	NotI-NotI
ACCN	NM_020172
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020172.1, NP_064557.1
RefSeq Size	1815 bp
RefSeq ORF	1815 bp
Locus ID	56928
Domains	PA, PSN, DUF435
Protein Families	Protease, Transmembrane
Gene Summary	This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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