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PIP5K3 (PIKFYVE) (NM_015040) Human Untagged Clone
CAT＃: SC121037
PIKFYVE (untagged)-Human phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant 2
CNY 13,856.00
货期*
现货
规格
Product images
经常一起买 (4)
PIKFYVE mouse monoclonal antibody,clone OTI1D2
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC121037 sequence for NM_015040 edited (data generated by NextGen Sequencing)
ATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAATGATTTGCCTCGATCT CCTACTAGTCCTTCTCATCTCACACACTTTAAACCTTTGACTCCTGATCAAGATGAGCCC CCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCTCTTTCGTTTTAACAAAGAGAGAGCA GAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGGAAGTTGGACCAGCCCTCAGCTCCCT TCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAAAGCAGCTTAATGAGGAACTC CAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCTACCTTTGGAGGTCAT GACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTAAAACGCCTCAAGGAA ATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAATACTGGATGCCAGATAGCCAA TGTAAAGAGTGCTATGACTGTAGTGAGAAATTTACAACCTTTAGGCGCAGACACCATTGC CGACTATGTGGGCAGATTTTCTGCAGTCGTTGCTGTAATCAAGAAATCCCTGGAAAATTT ATGGGCTATACAGGAGACCTCCGAGCTTGCACATATTGTAGAAAAATAGCCTTAAGTTAT GCTCATTCCACAGACAGTAATTCTATTGGGGAAGACTTGAATGCTCTTTCAGATTCTGCT TGCTCTGTGTCTGTGCTTGATCCAAGTGAACCCCGAACACCTGTTGGGAGTAGGAAAGCC AGCCGTAACATATTTTTAGAGGATGATTTGGCCTGGCAAAGTTTGATTCATCCAGATTCC TCAAATACTCCTCTTTCAACAAGACTTGTATCTGTGCAAGAGGATGCTGGGAAATCTCCT GCTCGAAATAGATCAGCCAGCATTACTAACCTGTCACTGGATAGATCTGGTTCTCCTATG GTACCTTCATATGAGACATCTGTCAGTCCCCAGGCTAACCGAACATATGTTAGGACAGAG ACCACTGAGGATGAACGCAAAATTCTTCTGGACAGTGTGCAGTTAAAAGACCTGTGGAAA AAAATCTGCCATCACAGCAGTGGAATGGAGTTTCAGGATCACCGCTACTGGTTGAGAACG CATCCCAACTGCATTGTAGGAAAGGAATTAGTCAACTGGCTAATCCGAAATGGGCATATT GCCACAAGGGCACAAGCTATAGCAATTGGACAAGCAATGGTTGATGGACGTTGGCTGGAT TGTGTTAGTCATCACGACCAGCTTTTCAGAGATGAGTATGCGCTGTATAGACCACTGCAG AGTACAGAATTTTCTGAGACGCCTTCTCCCGACAGTGACTCAGTGAACTCCGTGGAAGGA CACTCTGAGCCATCCTGGTTTAAAGACATAAAGTTTGATGACAGTGACACAGAACAGATA GCTGAAGAAGGTGACGATAATTTGGCTAATTCTGCCAGTCCTAGCAAGCGCACATCAGTC AGCAGTTTCCAGTCCACAGTGGACAGTGACTCAGCCGCTTCTATCAGCCTGAACGTGGAG CTGGACAACGTGAACTTCCATATCAAGAAGCCCTCCAAGTACCCACATGTGCCCCCTCAC CCTGCTGACCAAAAAGAGTATTTGATTTCTGACACTGGAGGACAACAGCTCTCAATAAGT GACGCTTTCATCAAAGAATCCTTATTTAATCGCCGAGTAGAGGAAAAATCCAAAGAGCTG CCTTTCACACCTTTGGGCTGGCATCATAACAACCTGGAGCTCCTGAGGGAGGAGAATGGG GAGAAACAAGCCATGGAGAGGTTGCTTTCAGCTAATCATAACCACATGATGGCACTACTC CAGCAGTTGCTCCATAGTGACTCACTGTCATCATCTTGGAGGGACATCATCGTGTCATTG GTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGTCAAGAACCAGGATGATGACATGGAT ATCCGTCAGTTTGTCCACATCAAAAAAATCCCAGGTGGAAAGAAGTTTGATTCTGTGGTT GTCAATGGCTTTGTTTGTACCAAGAACATTGCACATAAAAAGATGAATTCTTGTATTAAA AACCCTAAAATTCTTCTGTTGAAGTGTTCCATTGAGTATCTCTACAGAGAAGAAACTAAG TTTACTTGCATTGATCCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTCCAG CGAATAGTTGATGTTCGACCCACCTTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCC CAGGACATGTTATTGGAACATGGCATTACTTTGGTCATTAATGTAAAGTCACAAGTTTTG GAACGAATCAGTCGAATGACCCAAGGTGATTTAGTGATGTCAATGGACCAGCTGCTTACG AAACCACACCTGGGCACTTGTCACAAATTTTATATGCAGATATTTCAGTTGCCTAATGAA CAAACCAAGACACTGATGTTTTTTGAAGGTTGTCCACAGCACCTAGGCTGTACAATCAAG CTAAGAGGAGGCTCTGATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATC TGTGTTGCTTATCATTCTCAACTAGAAATATCCTTTCTCATGGATGAATTTGCTATGCCT CCCACATTAATGCAAAACCCTTCATTCCATTCCCTGATTGAGGGACGAGGGCATGAGGGG GCTGTCCAAGAGCAGTACGGTGGAGGTTCCATCCCCTGGGATCCTGACATCCCTCCTGAG TCTCTGCCCTGTGATGATAGCAGTTTGCTGGAATCGAGGATTGTGTTTGAGAAGGGTGAG CAGGAAAATAAAAATCTTCCGCAGGCTGTTGCCTCTGTGAAGCATCAAGAACATAGCACA ACAGCTTGCCCGGCGGGTCTCCCTTGTGCTTTCTTTGCACCTGTACCGGAATCATTGTTG CCACTCCCTGTGGATGACCAACAAGATGCTTTAGGCAGCGAGCTGCCAGAGAGTTTGCAG CAAACAGTTGTGCTGCAGGATCCCAAAAGCCAGATAAGAGCCTTTAGAGACCCTCTACAG GATGACACTGGATTATATGTTACTGAGGAAGTCACCTCCTCTGAAGATAAACGAAAGACT TATTCTTTGGCCTTTAAGCAGGAATTAAAAGATGTGATCCTCTGTATCTCCCCAGTAATC ACATTCCGAGAACCCTTTCTTTTAACTGAAAAGGGGATGAGATGCTCTACCCGAGATTAT TTTGCAGAGCAGGTTTACTGGTCTCCTCTCCTCAATAAAGAATTCAAAGAAATGGAGAAC AGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGAAGTATT CAGGCCAAGTCTATTCAAGTCTTACCCTCACATGAGCTAGTGAGCACTAGAATTGCTGAG CATCTGGGCGATAGCCAGAGCTTGGGTAGAATGCTGGCCGATTATCGAGCCAGAGGAGGA AGAATTCAGCCCAAAAATTCAGACCCTTTTGCTCATTCAAAGGATGCATCAAGTACTTCA AGTGGCAAATCAGGAAGCAAAAACGAGGGTGATGAAGAGAGAGGGCTTATTCTGAGTGAT GCTGTGTGGTCAACAAAGGTGGACTGTCTGAATCCCATTAATCACCAGAGACTTTGTGTG CTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGCTCCTAGTGCCTGTGTCAGTCCTTGG ATTGTAACAATGGAATTTTATGGAAAGAATGATCTTACATTAGGAATATTTTTAGAGAGA TACTGTTTCAGGCCTTCTTATCAGTGTCCAAGCATGTTCTGTGATACCCCCATGGTACAT CATATTCGGCGCTTTGTTCATGGCCAAGGCTGTGTGCAGATAATCCTGAAGGAGTTGGAT TCTCCAGTACCTGGATATCAGCATACAATTCTTACATATTCCTGGTGTAGAATCTGCAAA CAGGTAACACCAGTTGTTGCTCTTTCCAATGAGTCCTGGTCTATGTCATTTGCAAAATAC CTTGAACTTAGGTTTTATGGGCACCAGTATACTCGCAGAGCCAACGCTGAGCCCTGTGGT CACTCCATCCATCATGATTATCACCAGTATTTCTCCTATAACCAGATGGTGGCGTCTTTC AGTTATTCTCCCATTCGGCTTCTTGAAGTATGTGTTCCACTCCCCAAAATATTCATTAAG CGTCAGGCCCCATTAAAAGTGTCCCTTCTTCAGGATCTGAAGGACTTCTTTCAAAAAGTT TCACAGGTATATGTTGCCATTGATGAAAGACTTGCATCTTTGAAAACTGATACATTTAGT AAAACAAGAGAGGAAAAAATGGAAGATATTTTTGCACAGAAAGAGATGGAAGAAGGTGAG TTCAAGAACTGGATTGAGAAGATGCAAGCAAGGCTCATGTCTTCCTCTGTAGATACCCCT CAGCAACTGCAGTCGGTCTTTGAGTCACTCATTGCCAAGAAACAAAGTCTCTGTGAAGTG CTGCAAGCTTGGAATAACAGGTTGCAGGACCTTTTCCAACAGGAAAAGGGTAGAAAGAGA CCTTCAGTTCCTCCAAGTCCTGGAAGACTGAGACAAGGGGAAGAAAGCAAGATAAGTGCG ATGGATGCATCTCCACGGAATATTTCTCCAGGACTTCAGAATGGAGAAAAAGAGGATCGC TTCTTAACAACTTTGTCCAGCCAGAGCTCCACCAGTTCTACTCATCTCCAATTGCCTACG CCACCTGAAGTCATGTCTGAACAGTCAGTGGGAGGGCCCCCTGAGCTAGATACAGCCAGC AGTTCCGAAGATGTGTTTGATGGGCATTTGCTGGGATCCACAGACAGCCAAGTGAAGGAA AAGTCAACCATGAAAGCCATCTTTGCAAATTTGCTTCCAGGAAATAGCTATAATCCTATT CCATTTCCTTTTGATCCAGATAAACACTACTTAATGTATGAACATGAACGAGTGCCCATT GCAGTCTGCGAGAAGGAACCCAGCTCCATCATTGCTTTTGCTCTCAGTTGTAAAGAATAC CGAAATGCCTTAGAGGAATTGTCTAAAGCGACTCAGTGGAACAGTGCCGAAGAAGGGCTT CCAACAAATAGTACTTCAGATAGCAGACCAAAGAGTAGCAGCCCTATCAGATTACCTGAA ATGAGTGGAGGACAGACAAATCGTACAACAGAAACAGAACCACAACCAACCAAAAAGGCT TCTGGAATGTTGTCCTTCTTCAGAGGGACAGCAGGGAAAAGCCCCGATCTCTCTTCCCAG AAGAGAGAGACCTTACGTGGAGCAGATAGTGCTTACTACCAGGTTGGGCAGACAGGCAAG GAGGGGACCGAGAATCAAGGCGTTGAGCCTCAAGATGAAGTAGATGGAGGAGATACGCAA AAGAAGCAACTCATAAATCCTCATGTGGAACTTCAATTTTCAGATGCTAATGCCAAGTTT TACTGTCGGCTCTACTATGCGGGAGAGTTTCATAAGATGCGTGAAGTGATTCTGGACAGC AGTGAGGAAGATTTCATTCGTTCCCTCTCCCACTCATCACCCTGGCAGGCCCGGGGAGGC AAATCAGGAGCTGCCTTCTATGCAACTGAGGATGATAGATTTATTTTGAAGCAAATGCCT CGTCTGGAAGTCCAGTCCTTCCTCGACTTTGCACCACATTACTTCAATTATATTACAAAT GCTGTTCAACAAAAGAGGCCCACGGCGTTGGCCAAAATTCTTGGAGTTTACAGAATTGGT TATAAGAACTCTCAGAACAACACTGAGAAGAAGTTAGATCTCCTTGTCATGGAAAATCTT TTCTACGGGAGAAAGATGGCACAGGTTTTTGATTTGAAGGGCTCTCTTAGGAATCGGAAT GTAAAAACTGACACTGGAAAAGAGAGTTGTGATGTGGTCCTGCTAGATGAAAATCTCCTA AAGATGGTTCGAGACAACCCTCTATATATTCGTTCTCATTCCAAAGCTGTGCTGAGAACC TCGATCCATAGTGACTCCCATTTCCTTTCTAGCCACCTCATTATAGATTATTCTTTGCTG GTTGGGCGAGATGATACTAGCAATGAGCTAGTAGTTGGAATTATAGATTATATTCGAACA TTTACATGGGACAAAAAGCTTGAGATGGTTGTGAAATCAACAGGAATTTTAGGTGGACAA GGTAAAATGCCAACAGTGGTGTCTCCGGAGTTGTACAGGACTAGGTTTTGTGAGGCAATG GACAAGTATTTCCTAATGGTACCAGACCACTGGACAGGCTTGGGTCTGAATTGCTGA Clone variation with respect to NM_015040.3 2087 g=>a;2106 c=>t;2795 t=>c;2984 a=>t;2993 c=>g;3547 c=>a;3564 t=>c;5334 g=>a;5397 a=>g;5526 a=>g >OriGene 5' read for NM_015040 unedited
GGGTGCAAAATTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCTT CCTGCCGCAACCGTCCGCGGCCTGAGGAGCCCACCGCCGCTCTCGGGGGCCGACTTCCGG GGGCTGAGCCGTTGAAGCGGAGGCTGGGGCGGGGGGCAGCCGGCGCGGCCGGGGCAGGAG GCGCAGACTCATGAAATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAAT GATTTGCCTCGATCTCCTACTAGTCCTTCTCATCTCACACACTTTAAACCTTTGACTCCT GATCAAGATGAGCCCCCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCTCTTTCGTTTT AACAAAGAGAGAGCAGAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGGAAGTTGGACC AGCCCTCAGCTCCCTTCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAAAGCAG CTTAATGAGGAACTCCAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCT ACCTTTGGAGGTCATGACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTA AAACGCCTCAAGGAAATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAAACTGGA TGCCAGATAGCCAATGTAAAGAGTGCTATGACTGTAGTGAGAAATTACAACCTTTTAGCG CAGACACCATTGCCGACTATGTGGGCAGATTNTCTGCAGTCGTTGCTGTAATCAAGAAAT CCCCTGGAAAATTATGGGCTATACANGAGACCTNCGAGCTGCACATATTGTAGAAAATAG CCTTAGTATGCTCATTCCACAGACAGTATCTATGGGGGAAGACTGATGCTCTTCAGATCT GCTGCTCTNGGCTGGCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015040 |
| Insert Size | 6300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015040.2, NP_055855.1 |
| RefSeq Size | 9908 bp |
| RefSeq ORF | 6297 bp |
| Locus ID | 200576 |
| UniProt ID | Q9Y2I7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton |
| Gene Summary | Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010] Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). |
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